TY  - JOUR
A1  - Frasca, Stefano
A1  - Richter, Claudia
A1  - von Graberg, Till
A1  - Smarsly, Bernd M.
A1  - Wollenberger, Ursula
T1  - Electrochemical switchable protein-based optical device
JF  - Engineering in life sciences : Industry, Environment, Plant, Food
N2  - The present work contributes to the development of reusable sensing systems with a visual evaluation of the detection process related to an analyte. An electrochemical switchable protein-based optical device was designed with the core part composed of cytochrome c immobilized in a mesoporous indium tin oxide film. A color-developing redox-sensitive dye was used as switchable component of the system. The cytochrome c-catalyzed oxidation of the dye by hydrogen peroxide is spectroscopically investigated. When the dye is co-immobilized with the protein, its redox state is easily controlled by application of an electrical potential at the supporting material. This enables to electrochemically reset the system to the initial state and repetitive signal generation. The implemented reset function of the color forming reaction will make calibration of small test devices possible. The principle can be extended to other color forming redox reactions and to coupled enzyme systems, such as rapid food testing and indication of critical concentrations of metabolites for health care.
KW  - Cytochrome c
KW  - Electrochemical switch
KW  - Indium tin oxide
KW  - mesoporous materials
KW  - Optical device
Y1  - 2011
U6  - https://doi.org/10.1002/elsc.201100079
SN  - 1618-0240
VL  - 11
IS  - 6
SP  - 554
EP  - 558
PB  - Wiley-Blackwell
CY  - Malden
ER  -