TY  - JOUR
A1  - Baessler, Olivia Y.
A1  - Weiss, Julia
A1  - Wienkoop, Stefanie
A1  - Lehmann, Karola
A1  - Scheler, Christian
A1  - Doelle, Sabine
A1  - Schwarz, Dietmar
A1  - Franken, Philipp
A1  - George, Eckhard
A1  - Worm, Margitta
A1  - Weckwerth, Wolfram
T1  - Evidence for novel tomato seed allergens : IgE-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling
N2  - Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomato-sensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins.
Y1  - 2009
UR  - http://pubs.acs.org/journal/jprobs
U6  - https://doi.org/10.1021/Pr800186d
SN  - 1535-3893
ER  - 
TY  - JOUR
A1  - Garcia, Ada
A1  - Rühl, Ralph
A1  - Herz, U.
A1  - Koebnick, Corinna
A1  - Schweigert, Florian J.
A1  - Worm, Margitta
T1  - Retinoid- and carotenoid-enriched diets influence the ontogenesis of the immune system in mice
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Koch, Christin
A1  - Brovkina, Lioudmila
A1  - Rühl, Ralph
A1  - Worm, Margitta
T1  - Immunomodulation by dietary n3-polyunsaturated fatty acids (PUFA) in vitro
Y1  - 2006
SN  - 0091-6749
ER  - 
TY  - JOUR
A1  - Rühl, Ralph
A1  - Garcia, Ada
A1  - Schweigert, Florian J.
A1  - Worm, Margitta
T1  - Modulation of cytokine production by low and high retinoid diet in ovalbumin-sensitized mice
N2  - Retinoids modulate many physiological processes such as the differentiation and growth of different cell types. including cells from the immune system. We have previously shown that retinoids modulate IgE production in vitro and in vivo. In the present study we investigated the effects of retinoids in non-sensitized and ovalbumin-sensitized mice that were fed for 11 weeks with three different vitamin A (VA) diets: a) VA-deficiency diet, b) base diet, and c) base diet supplemented with 0.5% all-trans-retinoic acid (ATRA). Phorbol-myristate-acetate (PMA)/ionomycin-stimulated SMC (splenic mononuclear cells) from mice fed with ATRA and the vitamin A-deficient diet group showed increased interleukin-4 (IL-4) responses in non-sensitized mice. After ovalbumin sensitization in the VA-deficient and the ATRA supplementation diet groups, no significant effects on IL-4 production were observed. By contrast, gamma interferon (IFN-gamma production from PMA/ionomycin-stimulated SMC was enhanced in the VA-deficient diet group in ovalbumin-sensitized mice, and also in non-sensitized mice compared to the base and the ATRA-supplemented diet group. The data indicate that VA and retinoid content in a diet influences the cytokine response in non-sensitized and also ovalbumin-sensitized mice. Therefore these molecules may serve as active modulators of cytokine production in vivo that are responsible for the induction and persistence of atopic diseases
Y1  - 2004
ER  -