TY  - JOUR
A1  - Wang, Weiwei
A1  - Naolou, Toufik
A1  - Ma, Nan
A1  - Deng, Zijun
A1  - Xu, Xun
A1  - Mansfeld, Ulrich
A1  - Wischke, Christian
A1  - Gossen, Manfred
A1  - Neffe, Axel T.
A1  - Lendlein, Andreas
T1  - Polydepsipeptide Block-Stabilized Polyplexes for Efficient Transfection of Primary Human Cells
JF  - Biomacromolecules : an interdisciplinary journal focused at the interface of polymer science and the biological sciences
N2  - The rational design of a polyplex gene carrier aims to balance maximal effectiveness of nucleic acid transfection into cells with minimal adverse effects. Depsipeptide blocks with an M (n) similar to 5 kDa exhibiting strong physical interactions were conjugated with PEI moieties (2.5 or 10 kDa) to di- and triblock copolymers. Upon nanoparticle formation and complexation with DNA, the resulting polyplexes (sizes typically 60-150 nm) showed remarkable stability compared to PEI-only or lipoplex and facilitated efficient gene delivery. Intracellular trafficking was visualized by observing fluorescence-labeled pDNA and highlighted the effective cytoplasmic uptake of polyplexes and release of DNA to the perinuclear space. Specifically, a triblock copolymer with a middle depsipeptide block and two 10 kDa PEI swallowtail structures mediated the highest levels of transgenic VEGF secretion in mesenchymal stem cells with low cytotoxicity. These nanocarriers form the basis for a delivery platform technology, especially for gene transfer to primary human cells.
Y1  - 2017
U6  - https://doi.org/10.1021/acs.biomac.7b01034
SN  - 1525-7797
SN  - 1526-4602
VL  - 18
SP  - 3819
EP  - 3833
PB  - American Chemical Society
CY  - Washington
ER  -