TY  - JOUR
A1  - Lützow, Karola
A1  - Hommes-Schattmann, Paul J.
A1  - Neffe, Axel T.
A1  - Ahmad, Bilal
A1  - Williams, Gareth R.
A1  - Lendlein, Andreas
T1  - Perfluorophenyl azide functionalization of electrospun poly(para-dioxanone)
JF  - Polymers for advanced technologies
N2  - Strategies to surface-functionalize scaffolds by covalent binding of biologically active compounds are of fundamental interest to control the interactions between scaffolds and biomolecules or cells. Poly(para-dioxanone) (PPDO) is a clinically established polymer that has shown potential as temporary implant, eg, for the reconstruction of the inferior vena cava, as a nonwoven fiber mesh. However, PPDO lacks suitable chemical groups for covalent functionalization. Furthermore, PPDO is highly sensitive to hydrolysis, reflected by short in vivo half-life times and degradation during storage. Establishing a method for covalent functionalization without degradation of this hydrolyzable polymer is therefore important to enable the surface tailoring for tissue engineering applications. It was hypothesized that treatment of PPDO with an N-hydroxysuccinimide ester group bearing perfluorophenyl azide (PFPA) under UV irradiation would allow efficient surface functionalization of the scaffold. X-ray photoelectron spectroscopy and attenuated total reflectance Fourier-transformed infrared spectroscopy investigation revealed the successful binding, while a gel permeation chromatography study showed that degradation did not occur under these conditions. Coupling of a rhodamine dye to the N-hydroxysuccinimide esters on the surface of a PFPA-functionalized scaffold via its amine linker showed a homogenous staining of the PPDO in laser confocal microscopy. The PFPA method is therefore applicable even to the surface functionalization of hydrolytically labile polymers, and it was demonstrated that PFPA chemistry may serve as a versatile tool for the (bio-)functionalization of PPDO scaffolds.
KW  - biological applications of polymers
KW  - fibers
KW  - functionalization of polymers
KW  - microstructure
Y1  - 2018
U6  - https://doi.org/10.1002/pat.4331
SN  - 1042-7147
SN  - 1099-1581
VL  - 30
IS  - 5
SP  - 1165
EP  - 1172
PB  - Wiley
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Kedracki, Dawid
A1  - Filippov, Sergey K.
A1  - Gour, Nidhi
A1  - Schlaad, Helmut
A1  - Nardin, Corinne
T1  - Formation of DNA-Copolymer Fibrils Through an Amyloid-Like Nucleation Polymerization Mechanism
JF  - Macromolecular rapid communications
N2  - Conjugation of a hydrophobic poly(2-oxazoline) bearing tertiary amide groups along its backbone with a short single stranded nucleotide sequence results in an amphiphilic comb/graft copolymer, which organizes in fibrils upon direct dissolution in water. Supported by circular dichroism, atomic force microscopy, transmission electron microscopy, and scattering data, fibrils are formed through inter- and intramolecular hydrogen bonding between hydrogen accepting amide groups along the polymer backbone and hydrogen donating nucleic acid grafts leading to the formation of hollow tubes.
KW  - DNA copolymers
KW  - fibers
KW  - hydrogen bonding
KW  - nucleation polymerization
KW  - self-assembly
Y1  - 2015
U6  - https://doi.org/10.1002/marc.201400728
SN  - 1022-1336
SN  - 1521-3927
VL  - 36
IS  - 8
SP  - 768
EP  - 773
PB  - Wiley-VCH
CY  - Weinheim
ER  -