TY  - JOUR
A1  - Moradian, Hanieh
A1  - Gossen, Manfred
A1  - Lendlein, Andreas
T1  - Co-delivery of genes can be confounded by bicistronic vector design
JF  - MRS Communications
N2  - Maximizing the efficiency of nanocarrier-mediated co-delivery of genes for co-expression in the same cell is critical for many applications. Strategies to maximize co-delivery of nucleic acids (NA) focused largely on carrier systems, with little attention towards payload composition itself. Here, we investigated the effects of different payload designs: co-delivery of two individual "monocistronic" NAs versus a single bicistronic NA comprising two genes separated by a 2A self-cleavage site. Unexpectedly, co-delivery via the monocistronic design resulted in a higher percentage of co-expressing cells, while predictive co-expression via the bicistronic design remained elusive. Our results will aid the application-dependent selection of the optimal methodology for co-delivery of genes.
KW  - Molecular
KW  - Packaging
KW  - Protein
Y1  - 2022
U6  - https://doi.org/10.1557/s43579-021-00128-7
SN  - 2159-6859
SN  - 2159-6867
VL  - 12
IS  - 2
SP  - 145
EP  - 153
PB  - Springer
CY  - Heidelberg
ER  -