TY  - JOUR
A1  - Wawrzinek, Robert
A1  - Wessig, Pablo
A1  - Möllnitz, Kristian
A1  - Nikolaus, Joerg
A1  - Schwarzer, Roland
A1  - Müller, Peter
A1  - Herrmann, Andreas
T1  - DBD dyes as fluorescent probes for sensing lipophilic environments
JF  - Bioorganic & medicinal chemistry letters : a Tetrahedron publication for rapid dissemination of preliminary communications on all aspects of bioorganic chemistry, medicinal chemistry and related disciplines
N2  - Small fluorescent organic molecules based on [1,3]dioxolo[4,5-f][1,3]benzodioxole (DBD) could be used as probes for lipophillic microenvironments in aqueous solutions by indicating the critical micelles concentration of detergents and staining cell organelles. Their fluorescence lifetime decreases drastically by the amount of water in their direct environment. Therefore they are potential probes for fluorescence lifetime imaging microscopy (FLIM).
KW  - Fluorescence lifetime probes
KW  - FLIM
KW  - Cell staining
KW  - Lysotrackers
KW  - Detergents
Y1  - 2012
U6  - https://doi.org/10.1016/j.bmcl.2012.07.056
SN  - 0960-894X
VL  - 22
IS  - 17
SP  - 5367
EP  - 5371
PB  - Elsevier
CY  - Oxford
ER  - 
TY  - CHAP
A1  - Haralampiev, Ivan
A1  - Mertens, Monique
A1  - Schwarzer, Roland
A1  - Herrmann, Andreas
A1  - Volkmer, Rudolf
A1  - Wessig, Pablo
A1  - Müller, Peter
T1  - A palmitic acid functionalized with a maleimide group is used to recruit SH-containing peptides to lipid and biological membranes
T2  - The FEBS journal
Y1  - 2015
SN  - 1742-464X
SN  - 1742-4658
VL  - 282
SP  - 204
EP  - 204
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Haralampiev, Ivan
A1  - Mertens, Monique
A1  - Schwarzer, Roland
A1  - Herrmann, Andreas
A1  - Volkmer, Rudolf
A1  - Wessig, Pablo
A1  - Mueller, Peter
T1  - Recruitment of SH-Containing peptides to lipid and biological membranes through the use of a palmitic acid functionalized with a Maleimide Group
JF  - Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition
N2  - This study presents a novel and easily applicable approach to recruit sulfhydryl-containing biomolecules to membranes by using a palmitic acid which is functionalized with a maleimide group. Notably, this strategy can also be employed with preformed (biological) membranes. The applicability of the assay is demonstrated by characterizing the binding of a Rhodamine-labeled peptide to lipid and cellular membranes using methods of fluorescence spectroscopy, lifetime measurement, and microscopy. Our approach offers new possibilities for preparing biologically active liposomes and manipulating living cells.
KW  - liposomes
KW  - maleimide
KW  - membranes
KW  - palmitic acid
KW  - palmitoylation
KW  - peptides
Y1  - 2015
U6  - https://doi.org/10.1002/anie.201408089
SN  - 1433-7851
SN  - 1521-3773
VL  - 54
IS  - 1
SP  - 323
EP  - 326
PB  - Wiley-VCH
CY  - Weinheim
ER  -