TY  - GEN
A1  - Naolou, Toufik
A1  - Rühl, Eckart
A1  - Lendlein, Andreas
T1  - Nanocarriers
BT  - Architecture, transport, and topical application of drugs for therapeutic use
T2  - European Journal of Pharmaceutics and Biopharmaceutics
Y1  - 2017
U6  - https://doi.org/10.1016/j.ejpb.2017.03.004
SN  - 0939-6411
SN  - 1873-3441
VL  - 116
SP  - 1
EP  - 3
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Wanjiku, Barbara
A1  - Yamamoto, Kenji
A1  - Klossek, Andre
A1  - Schumacher, Fabian
A1  - Pischon, Hannah
A1  - Mundhenk, Lars
A1  - Rancan, Fiorenza
A1  - Judd, Martyna M.
A1  - Ahmed, Muniruddin
A1  - Zoschke, Christian
A1  - Kleuser, Burkhard
A1  - Rühl, Eckart
A1  - Schäfer-Korting, Monika
T1  - Qualifying X-ray and Stimulated Raman Spectromicroscopy for Mapping Cutaneous Drug Penetration
JF  - Analytical chemistry
N2  - Research on topical drug delivery relies on reconstructed human skin (RHS) in addition to ex vivo human and animal skin, each with specific physiological features. Here, we compared the penetration of dexamethasone from an ethanolic hydroxyethyl cellulose gel into ex vivo human skin, murine skin, and RHS. For comprehensive insights into skin morphology and penetration enhancing mechanisms, scanning transmission X-ray microscopy (STXM), liquid chromatography tandem mass spectrometry (LC-MS/MS), and stimulated Raman spectromicroscopy (SRS) were combined. STXM offers high spatial resolution with label-free drug detection and is therefore sensitive to tissue damage. Despite differences in sample preparation and data analysis, the amounts of dexamethasone in RHS, detected and quantified by STXM and LC-MS/MS, were very similar and increased during the first 100 min of exposure. SRS revealed interactions between the gel and the stratum corneum or, more specifically, its protein and lipid structures. Similar to both types of ex vivo skin, higher protein-to-lipid ratios within the stratum corneum of RHS indicated reduced lipid amounts after 30 min of ethanol exposure. Extended ethanol exposure led to a continued reduction of lipids in the ex vivo matrixes, while protein integrity appeared to be compromised in RHS, which led to declining protein signals. In conclusion, LC-MS/MS proved the predictive capability of STXM for label-free drug detection. Combining STXM with SRS precisely dissected the penetration enhancing effects of ethanol. Further studies on topical drug delivery should consider the potential of these complementary techniques.
Y1  - 2019
U6  - https://doi.org/10.1021/acs.analchem.9b00519
SN  - 0003-2700
SN  - 1520-6882
VL  - 91
IS  - 11
SP  - 7208
EP  - 7214
PB  - American Chemical Society
CY  - Washington
ER  - 
TY  - CHAP
A1  - Frombach, Janna
A1  - Rancan, Fiorenza
A1  - Fleige, Emanuel
A1  - Haag, Rainer
A1  - Schumacher, Frank
A1  - Kleuser, Burkhard
A1  - Yamamoto, Kenji
A1  - Rühl, Eckart
A1  - Blume-Peytavi, Ulrike
A1  - Vogt, Annika
T1  - Skin penetration and dexamethasone release from polymer nanoparticles in ex vivo human skin
T2  - The journal of investigative dermatology
Y1  - 2015
SN  - 0022-202X
SN  - 1523-1747
VL  - 135
SP  - S52
EP  - S52
PB  - Nature Publ. Group
CY  - New York
ER  -