TY  - JOUR
A1  - Dai, Xiaolin
A1  - Mate, Diana M.
A1  - Glebe, Ulrich
A1  - Garakani, Tayebeh Mirzaei
A1  - Körner, Andrea
A1  - Schwaneberg, Ulrich
A1  - Böker, Alexander
T1  - Sortase-mediated ligation of purely artificial building blocks
JF  - Polymers
N2  - Sortase A (SrtA) from Staphylococcus aureus has been often used for ligating a protein with other natural or synthetic compounds in recent years. Here we show that SrtA-mediated ligation (SML) is universally applicable for the linkage of two purely artificial building blocks. Silica nanoparticles (NPs), poly(ethylene glycol) and poly(N-isopropyl acrylamide) are chosen as synthetic building blocks. As a proof of concept, NP-polymer, NP-NP, and polymer-polymer structures are formed by SrtA catalysis. Therefore, the building blocks are equipped with the recognition sequence needed for SrtA reaction-the conserved peptide LPETG-and a pentaglycine motif. The successful formation of the reaction products is shown by means of transmission electron microscopy (TEM), matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS), and dynamic light scattering (DLS). The sortase catalyzed linkage of artificial building blocks sets the stage for the development of a new approach to link synthetic structures in cases where their synthesis by established chemical methods is complicated.
KW  - sortase-mediated ligation
KW  - enzymes
KW  - block copolymers
KW  - nanoparticles
Y1  - 2018
U6  - https://doi.org/10.3390/polym10020151
SN  - 2073-4360
VL  - 10
IS  - 2
PB  - MDPI
CY  - Basel
ER  -