TY  - JOUR
A1  - Gräf, Ralph
A1  - Batsios, Petros
A1  - Meyer, Irene
T1  - Evolution of centrosomes and the nuclear lamina: Amoebozoan assets
JF  - European journal of cell biology
N2  - The current eukaryotic tree of life groups most eukaryotes into one of five supergroups, the Opisthokonta, Amoebozoa, Archaeplastida, Excavata and SAR (Stramenopile, Alveolata, Rhizaria). Molecular and comparative morphological analyses revealed that the last eukaryotic common ancestor (LECA) already contained a rather sophisticated equipment of organelles including a mitochondrion, an endomembrane system, a nucleus with a lamina, a microtubule-organizing center (MTOC), and a flagellar apparatus. Recent studies of MTOCs, basal bodies/centrioles, and nuclear envelope organization of organisms in different supergroups have clarified our picture of how the nucleus and MTOCs co-evolved from LECA to extant eukaryotes. In this review we summarize these findings with special emphasis on valuable contributions of research on a lamin-like protein, nuclear envelope proteins, and the MTOC in the amoebozoan model organism Dictyostelium discoideum. (C) 2015 Elsevier GmbH. All rights reserved.
KW  - LINC complex
KW  - Sun1
KW  - Nuclear lamina
KW  - Lamin
KW  - Nuclear envelope
KW  - Centrosome
KW  - Basal body
KW  - Centriole
KW  - LEM-domain
Y1  - 2015
U6  - https://doi.org/10.1016/j.ejcb.2015.04.004
SN  - 0171-9335
SN  - 1618-1298
VL  - 94
IS  - 6
SP  - 249
EP  - 256
PB  - Elsevier
CY  - Jena
ER  - 
TY  - JOUR
A1  - Schweigel, Ulrike
A1  - Batsios, Petros
A1  - Müller-Taubenberger, Annette
A1  - Gräf, Ralph
A1  - Grafe, Marianne
T1  - Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis
JF  - Nucleus
N2  - Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates to the cytoplasmic side of the nucleus, the central spindle disassembles and the nuclear fenestrae become closed. Our data indicate that Dictyostelium spastin (DdSpastin) is a microtubule-binding and severing type I membrane protein that plays a role in this process. Its mitotic localization is in agreement with a requirement for the removal of microtubules that would hinder closure of the fenestrae. Furthermore, DdSpastin interacts with the HeH/ LEM-family protein Src1 in BioID analyses as well as the inner nuclear membrane protein Sun1, and shows subcellular co-localizations with Src1, Sun1, the ESCRT component CHMP7 and the IST1-like protein filactin, suggesting that the principal pathway of mitotic nuclear envelope remodeling is conserved between animals and Dictyostelium amoebae.
KW  - Spastin
KW  - LEM-domain
KW  - ESCRT
KW  - sun1
KW  - dictyostelium
KW  - nuclear envelope
KW  - mitosis
Y1  - 2022
U6  - https://doi.org/10.1080/19491034.2022.2047289
SN  - 1949-1034
SN  - 1949-1042
VL  - 13
IS  - 1
SP  - 144
EP  - 154
PB  - Taylor & Francis Group
CY  - Philadelphia
ER  -