TY  - JOUR
A1  - Liu, Qi
A1  - Adler, Karsten
A1  - Lipus, Daniel
A1  - Kämpf, Horst
A1  - Bussert, Robert
A1  - Plessen, Birgit
A1  - Schulz, Hans-Martin
A1  - Krauze, Patryk
A1  - Horn, Fabian
A1  - Wagner, Dirk
A1  - Mangelsdorf, Kai
A1  - Alawi, Mashal
T1  - Microbial signatures in deep CO2-saturated miocene sediments of the active Hartousov mofette system (NW Czech Republic)
JF  - Frontiers in microbiology
N2  - The Hartousov mofette system is a natural CO2 degassing site in the central Cheb Basin (Eger Rift, Central Europe). In early 2016 a 108 m deep core was obtained from this system to investigate the impact of ascending mantle-derived CO2 on indigenous deep microbial communities and their surrounding life habitat. During drilling, a CO2 blow out occurred at a depth of 78.5 meter below surface (mbs) suggesting a CO2 reservoir associated with a deep low-permeable CO2-saturated saline aquifer at the transition from Early Miocene terrestrial to lacustrine sediments. Past microbial communities were investigated by hopanoids and glycerol dialkyl glycerol tetraethers (GDGTs) reflecting the environmental conditions during the time of deposition rather than showing a signal of the current deep biosphere. The composition and distribution of the deep microbial community potentially stimulated by the upward migration of CO2 starting during Mid Pleistocene time was investigated by intact polar lipids (IPLs), quantitative polymerase chain reaction (qPCR), and deoxyribonucleic acid (DNA) analysis. The deep biosphere is characterized by microorganisms that are linked to the distribution and migration of the ascending CO2-saturated groundwater and the availability of organic matter instead of being linked to single lithological units of the investigated rock profile. Our findings revealed high relative abundances of common soil and water bacteria, in particular the facultative, anaerobic and potential iron-oxidizing Acidovorax and other members of the family Comamonadaceae across the whole recovered core. The results also highlighted the frequent detection of the putative sulfate-oxidizing and CO2-fixating genus Sulfuricurvum at certain depths. A set of new IPLs are suggested to be indicative for microorganisms associated to CO2 accumulation in the mofette system.
KW  - geo-bio interaction
KW  - CO2
KW  - mofette systems
KW  - Eger Rift
KW  - microbial lipid
KW  - biomarker
KW  - microbial diversity
KW  - deep biosphere
KW  - saline groundwater
Y1  - 2020
U6  - https://doi.org/10.3389/fmicb.2020.543260
SN  - 1664-302X
VL  - 11
PB  - Frontiers Media
CY  - Lausanne
ER  -