TY - JOUR A1 - Baritello, Omar A1 - Khajooei, Mina A1 - Engel, Tilman A1 - Kopinski, Stephan A1 - Quarmby, Andrew James A1 - Müller, Steffen A1 - Mayer, Frank T1 - Neuromuscular shoulder activity during exercises with different combinations of stable and unstable weight mass JF - BMC sports science, medicine and rehabilitation N2 - Background Recent shoulder injury prevention programs have utilized resistance exercises combined with different forms of instability, with the goal of eliciting functional adaptations and thereby reducing the risk of injury. However, it is still unknown how an unstable weight mass (UWM) affects the muscular activity of the shoulder stabilizers. Aim of the study was to assess neuromuscular activity of dynamic shoulder stabilizers under four conditions of stable and UWM during three shoulder exercises. It was hypothesized that a combined condition of weight with UWM would elicit greater activation due to the increased stabilization demand. Methods Sixteen participants (7 m/9 f) were included in this cross-sectional study and prepared with an EMG-setup for the: Mm. upper/lower trapezius (U.TA/L.TA), lateral deltoid (DE), latissimus dorsi (LD), serratus anterior (SA) and pectoralis major (PE). A maximal voluntary isometric contraction test (MVIC; 5 s.) was performed on an isokinetic dynamometer. Next, internal/external rotation (In/Ex), abduction/adduction (Ab/Ad) and diagonal flexion/extension (F/E) exercises (5 reps.) were performed with four custom-made-pipes representing different exercise conditions. First, the empty-pipe (P; 0.5 kg) and then, randomly ordered, water-filled-pipe (PW; 1 kg), weight-pipe (PG; 4.5 kg) and weight + water-filled-pipe (PWG; 4.5 kg), while EMG was recorded. Raw root-mean-square values (RMS) were normalized to MVIC (%MVIC). Differences between conditions for RMS%MVIC, scapular stabilizer (SR: U.TA/L.TA; U.TA/SA) and contraction (CR: concentric/eccentric) ratios were analyzed (paired t-test; p <= 0.05; Bonferroni adjusted alpha = 0.008). Results PWG showed significantly greater muscle activity for all exercises and all muscles except for PE compared to P and PW. Condition PG elicited muscular activity comparable to PWG (p > 0.008) with significantly lower activation of L.TA and SA in the In/Ex rotation. The SR ratio was significantly higher in PWG compared to P and PW. No significant differences were found for the CR ratio in all exercises and for all muscles. Conclusion Higher weight generated greater muscle activation whereas an UWM raised the neuromuscular activity, increasing the stabilization demands. Especially in the In/Ex rotation, an UWM increased the RMS%MVIC and SR ratio. This might improve training effects in shoulder prevention and rehabilitation programs. KW - EMG KW - instability KW - overhead athlete KW - unstable resistance training KW - water KW - pipe KW - rotator cuff Y1 - 2020 U6 - https://doi.org/10.1186/s13102-020-00168-x SN - 2052-1847 VL - 12 IS - 1 PB - BioMed Central CY - London ER - TY - JOUR A1 - Lehr, Christian A1 - Lischeid, Gunnar T1 - Efficient screening of groundwater head monitoring data for anthropogenic effects and measurement errors JF - Hydrology and Earth System Sciences N2 - Groundwater levels are monitored by environmental agencies to support the sustainable use of groundwater resources. For this purpose continuous and spatially comprehensive monitoring in high spatial and temporal resolution is desired. This leads to large datasets that have to be checked for quality and analysed to distinguish local anthropogenic influences from natural variability of the groundwater level dynamics at each well. Both technical problems with the measurements as well as local anthropogenic influences can lead to local anomalies in the hydrographs. We suggest a fast and efficient screening method for the identification of well-specific peculiarities in hydrographs of groundwater head monitoring networks. The only information required is a set of time series of groundwater heads all measured at the same instants of time. For each well of the monitoring network a reference hydrograph is calculated, describing expected "normal" behaviour at the respective well as is typical for the monitored region. The reference hydrograph is calculated by multiple linear regression of the observed hydrograph with the "stable" principal components (PCs) of a principal component analysis of all groundwater head series of the network as predictor variables. The stable PCs are those PCs which were found in a random subsampling procedure to be rather insensitive to the specific selection of the analysed observation wells, i.e. complete series, and to the specific selection of measurement dates. Hence they can be considered to be representative for the monitored region in the respective period. The residuals of the reference hydrograph describe local deviations from the normal behaviour. Peculiarities in the residuals allow the data to be checked for measurement errors and the wells with a possible anthropogenic influence to be identified. The approach was tested with 141 groundwater head time series from the state authority groundwater monitoring network in northeastern Germany covering the period from 1993 to 2013 at an approximately weekly frequency of measurement. KW - streamflow variability KW - principal components KW - united states KW - time-seriesa KW - water KW - network KW - nonstationarity KW - fluctuations KW - rotation Y1 - 2020 U6 - https://doi.org/10.5194/hess-24-501-2020 SN - 1027-5606 SN - 1607-7938 VL - 24 IS - 2 SP - 501 EP - 513 PB - Copernicus CY - Göttingen ER - TY - GEN A1 - Block, Inga A1 - Günter, Christina A1 - Rodrigues, Alysson Duarte A1 - Paasch, Silvia A1 - Hesemann, Peter A1 - Taubert, Andreas T1 - Carbon Adsorbents from Spent Coffee for Removal of Methylene Blue and Methyl Orange from Water T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Activated carbons (ACs) were prepared from dried spent coffee (SCD), a biological waste product, to produce adsorbents for methylene blue (MB) and methyl orange (MO) from aqueous solution. Pre-pyrolysis activation of SCD was achieved via treatment of the SCD with aqueous sodium hydroxide solutions at 90 °C. Pyrolysis of the pretreated SCD at 500 °C for 1 h produced powders with typical characteristics of AC suitable and effective for dye adsorption. As an alternative to the rather harsh base treatment, calcium carbonate powder, a very common and abundant resource, was also studied as an activator. Mixtures of SCD and CaCO3 (1:1 w/w) yielded effective ACs for MO and MB removal upon pyrolysis needing only small amounts of AC to clear the solutions. A selectivity of the adsorption process toward anionic (MO) or cationic (MB) dyes was not observed. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1155 KW - water KW - spent coffee KW - dye adsorption KW - methylene blue KW - methyl orange KW - calcium carbonate KW - activated carbon KW - water treatment KW - dye removal Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-521653 SN - 1866-8372 IS - 14 ER - TY - JOUR A1 - Block, Inga A1 - Günter, Christina A1 - Rodrigues, Alysson Duarte A1 - Paasch, Silvia A1 - Hesemann, Peter A1 - Taubert, Andreas T1 - Carbon Adsorbents from Spent Coffee for Removal of Methylene Blue and Methyl Orange from Water JF - Materials N2 - Activated carbons (ACs) were prepared from dried spent coffee (SCD), a biological waste product, to produce adsorbents for methylene blue (MB) and methyl orange (MO) from aqueous solution. Pre-pyrolysis activation of SCD was achieved via treatment of the SCD with aqueous sodium hydroxide solutions at 90 °C. Pyrolysis of the pretreated SCD at 500 °C for 1 h produced powders with typical characteristics of AC suitable and effective for dye adsorption. As an alternative to the rather harsh base treatment, calcium carbonate powder, a very common and abundant resource, was also studied as an activator. Mixtures of SCD and CaCO3 (1:1 w/w) yielded effective ACs for MO and MB removal upon pyrolysis needing only small amounts of AC to clear the solutions. A selectivity of the adsorption process toward anionic (MO) or cationic (MB) dyes was not observed. KW - water KW - spent coffee KW - dye adsorption KW - methylene blue KW - methyl orange KW - calcium carbonate KW - activated carbon KW - water treatment KW - dye removal Y1 - 2021 U6 - https://doi.org/10.3390/ma14143996 SN - 1996-1944 VL - 14 IS - 14 PB - MDPI CY - Basel ER - TY - JOUR A1 - Ugwuja, Chidinma G. A1 - Adelowo, Olawale O. A1 - Ogunlaja, Aemere A1 - Omorogie, Martins O. A1 - Olukanni, Olumide D. A1 - Ikhimiukor, Odion O. A1 - Iermak, Ievgeniia A1 - Kolawole, Gabriel A. A1 - Günter, Christina A1 - Taubert, Andreas A1 - Bodede, Olusola A1 - Moodley, Roshila A1 - Inada, Natalia M. A1 - Camargo, Andrea S.S. de A1 - Unuabonah, Emmanuel Iyayi T1 - Visible-Light-Mediated Photodynamic Water Disinfection @ Bimetallic-Doped Hybrid Clay Nanocomposites JF - ACS applied materials & interfaces N2 - This study reports a new class of photocatalytic hybrid clay nanocomposites prepared from low-cost sources (kaolinite clay and Carica papaya seeds) doped with Zn and Cu salts via a solvothermal process. X-ray diffraction analysis suggests that Cu-doping and Cu/Zn-doping introduce new phases into the crystalline structure of Kaolinite clay, which is linked to the reduced band gap of kaolinite from typically between 4.9 and 8.2 eV to 2.69 eV for Cu-doped and 1.5 eV for Cu/Zn hybrid clay nanocomposites (Nisar, J.; Arhammar, C.; Jamstorp, E.; Ahuja, R. Phys. Rev. B 2011, 84, 075120). In the presence of solar light irradiation, Cu- and Cu/Zn-doped nanocomposites facilitate the electron hole pair separation. This promotes the generation of singlet oxygen which in turn improves the water disinfection efficiencies of these novel nanocomposite materials. The nanocomposite materials were further characterized using high-resolution scanning electron microscopy, fluorimetry, therrnogravimetric analysis, and Raman spectroscopy. The breakthrough times of the nanocomposites for a fixed bed mode of disinfection of water contaminated with 2.32 x 10(7) cfu/mL E. coli ATCC 25922 under solar light irradiation are 25 h for Zn-doped, 30 h for Cu-doped, and 35 h for Cu/Zn-doped nanocomposites. In the presence of multidrug and multimetal resistant strains of E. coli, the breakthrough time decreases significantly. Zn-only doped nanocomposites are not photocatalytically active. In the absence of light, the nanocomposites are still effective in decontaminating water, although less efficient than under solar light irradiation. Electrostatic interaction, metal toxicity, and release of singlet oxygen (only in the Cu-doped and Cu/Zn-doped nanocomposites) are the three disinfection mechanisms by which these nanocomposites disinfect water. A regrowth study indicates the absence of any living E. coli cells in treated water even after 4 days. These data and the long hydraulic times (under gravity) exhibited by these nanocomposites during photodisinfection of water indicate an unusually high potential of these nanocomposites as efficient, affordable, and sustainable point-of-use systems for the disinfection of water in developing countries. KW - disinfection KW - nanocomposite material KW - multidrug-resistant Escherichia coli KW - water KW - reactive oxygen species Y1 - 2019 U6 - https://doi.org/10.1021/acsami.9b01212 SN - 1944-8244 SN - 1944-8252 VL - 11 IS - 28 SP - 25483 EP - 25494 PB - American Chemical Society CY - Washington, DC ER - TY - GEN A1 - Lara, Mark J. A1 - Nitze, Ingmar A1 - Große, Guido A1 - McGuire, David T1 - Tundra landform and vegetation productivity trend maps for the Arctic Coastal Plain of northern Alaska T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Arctic tundra landscapes are composed of a complex mosaic of patterned ground features, varying in soil moisture, vegetation composition, and surface hydrology over small spatial scales (10-100 m). The importance of microtopography and associated geomorphic landforms in influencing ecosystem structure and function is well founded, however, spatial data products describing local to regional scale distribution of patterned ground or polygonal tundra geomorphology are largely unavailable. Thus, our understanding of local impacts on regional scale processes (e.g., carbon dynamics) may be limited. We produced two key spatiotemporal datasets spanning the Arctic Coastal Plain of northern Alaska (similar to 60,000 km(2)) to evaluate climate-geomorphological controls on arctic tundra productivity change, using (1) a novel 30m classification of polygonal tundra geomorphology and (2) decadal-trends in surface greenness using the Landsat archive (1999-2014). These datasets can be easily integrated and adapted in an array of local to regional applications such as (1) upscaling plot-level measurements (e.g., carbon/energy fluxes), (2) mapping of soils, vegetation, or permafrost, and/or (3) initializing ecosystem biogeochemistry, hydrology, and/or habitat modeling. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1035 KW - spatial-distribution KW - lake basins KW - microtopography KW - water KW - ice KW - accumulation KW - degradation KW - permafrost KW - dynamics KW - barrow Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-459875 SN - 1866-8372 IS - 1035 ER - TY - GEN A1 - Duy, Nguyen Le A1 - Heidbüchel, Ingo A1 - Meyer, Hanno A1 - Merz, Bruno A1 - Apel, Heiko T1 - What controls the stable isotope composition of precipitation in the Mekong Delta? BT - a model-based statistical approach T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - This study analyzes the influence of local and regional climatic factors on the stable isotopic composition of rainfall in the Vietnamese Mekong Delta (VMD) as part of the Asian monsoon region. It is based on 1.5 years of weekly rainfall samples. In the first step, the isotopic composition of the samples is analyzed by local meteoric water lines (LMWLs) and single-factor linear correlations. Additionally, the contribution of several regional and local factors is quantified by multiple linear regression (MLR) of all possible factor combinations and by relative importance analysis. This approach is novel for the interpretation of isotopic records and enables an objective quantification of the explained variance in isotopic records for individual factors. In this study, the local factors are extracted from local climate records, while the regional factors are derived from atmospheric backward trajectories of water particles. The regional factors, i.e., precipitation, temperature, relative humidity and the length of backward trajectories, are combined with equivalent local climatic parameters to explain the response variables delta O-18, delta H-2, and d-excess of precipitation at the station of measurement. The results indicate that (i) MLR can better explain the isotopic variation in precipitation (R-2 = 0.8) compared to single-factor linear regression (R-2 = 0.3); (ii) the isotopic variation in precipitation is controlled dominantly by regional moisture regimes (similar to 70 %) compared to local climatic conditions (similar to 30 %); (iii) the most important climatic parameter during the rainy season is the precipitation amount along the trajectories of air mass movement; (iv) the influence of local precipitation amount and temperature is not sig-nificant during the rainy season, unlike the regional precipitation amount effect; (v) secondary fractionation processes (e.g., sub-cloud evaporation) can be identified through the d-excess and take place mainly in the dry season, either locally for delta O-18 and delta H-2, or along the air mass trajectories for d-excess. The analysis shows that regional and local factors vary in importance over the seasons and that the source regions and transport pathways, and particularly the climatic conditions along the pathways, have a large influence on the isotopic composition of rainfall. Although the general results have been reported qualitatively in previous studies (proving the validity of the approach), the proposed method provides quantitative estimates of the controlling factors, both for the whole data set and for distinct seasons. Therefore, it is argued that the approach constitutes an advancement in the statistical analysis of isotopic records in rainfall that can supplement or precede more complex studies utilizing atmospheric models. Due to its relative simplicity, the method can be easily transferred to other regions, or extended with other factors. The results illustrate that the interpretation of the isotopic composition of precipitation as a recorder of local climatic conditions, as for example performed for paleorecords of water isotopes, may not be adequate in the southern part of the Indochinese Peninsula, and likely neither in other regions affected by monsoon processes. However, the presented approach could open a pathway towards better and seasonally differentiated reconstruction of paleoclimates based on isotopic records. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 927 KW - Asian summer monsoon KW - interannuel variability KW - climate controls KW - deuterium excess KW - oxygen isotopes KW - paleoclimate KW - water KW - system KW - trajectories KW - circulation Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-445743 SN - 1866-8372 IS - 927 SP - 1239 EP - 1262 ER - TY - JOUR A1 - Neill, Christopher A1 - Jankowski, KathiJo A1 - Brando, Paulo M. A1 - Coe, Michael T. A1 - Deegan, Linda A. A1 - Macedo, Marcia N. A1 - Riskin, Shelby H. A1 - Porder, Stephen A1 - Elsenbeer, Helmut A1 - Krusche, Alex V. T1 - Surprisingly Modest Water Quality Impacts From Expansion and Intensification of Large-Sscale Commercial Agriculture in the Brazilian Amazon-Cerrado Region JF - Tropical conservation science N2 - Large-scale commercial cropping of soybeans expanded in the tropical Amazon and Cerrado biomes of Brazil after 1990. More recently, cropping intensified from single-cropping of soybeans to double-cropping of soybeans with corn or cotton. Cropland expansion and intensification, and the accompanying use of mineral fertilizers, raise concerns about whether nutrient runoff and impacts to surface waters will be similar to those experienced in commercial cropland regions at temperate latitudes. We quantified water infiltration through soils, water yield, and streamwater chemistry in watersheds draining native tropical forest and single-and double-cropped areas on the level, deep, highly weathered soils where cropland expansion and intensification typically occurs. Although water yield increased four-fold from croplands, streamwater chemistry remained largely unchanged. Soil characteristics exerted important control over the movement of nitrogen (N) and phosphorus (P) into streams. High soil infiltration rates prevented surface erosion and movement of particulate P, while P fixation in surface soils restricted P movement to deeper soil layers. Nitrogen retention in deep soils, likely by anion exchange, also appeared to limit N leaching and export in streamwater from both single-and double-cropped watersheds that received nitrogen fertilizer. These mechanisms led to lower streamwater P and N concentrations and lower watershed N and P export than would be expected, based on studies from temperate croplands with similar cropping and fertilizer application practices. KW - water KW - quality KW - agriculture KW - intensification KW - impact Y1 - 2017 U6 - https://doi.org/10.1177/1940082917720669 SN - 1940-0829 VL - 10 PB - Sage Publ. CY - Thousand Oaks ER - TY - GEN A1 - Fondell, Mattis A1 - Eckert, Sebastian A1 - Jay, Raphael Martin A1 - Weniger, Christian A1 - Quevedo, Wilson A1 - Niskanen, Johannes A1 - Kennedy, Brian A1 - Sorgenfrei, Nomi A1 - Schick, Daniel A1 - Giangrisostomi, Erika A1 - Ovsyannikov, Ruslan A1 - Adamczyk, Katrin A1 - Huse, Nils A1 - Wernet, Philippe A1 - Mitzner, Rolf A1 - Föhlisch, Alexander T1 - Time-resolved soft X-ray absorption spectroscopy in transmission mode on liquids at MHz repetition rates T2 - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe N2 - We present a setup combining a liquid flatjet sample delivery and a MHz laser system for time-resolved soft X-ray absorption measurements of liquid samples at the high brilliance undulator beamline UE52-SGM at Bessy II yielding unprecedented statistics in this spectral range. We demonstrate that the efficient detection of transient absorption changes in transmission mode enables the identification of photoexcited species in dilute samples. With iron(II)-trisbipyridine in aqueous solution as a benchmark system, we present absorption measurements at various edges in the soft X-ray regime. In combination with the wavelength tunability of the laser system, the set-up opens up opportunities to study the photochemistry of many systems at low concentrations, relevant to materials sciences, chemistry, and biology. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 780 KW - l-edge xas KW - electronic-structure KW - molecular-structure KW - spin-state KW - dynamics KW - complexes KW - probe KW - water KW - iron(II) KW - spectra Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-437529 SN - 1866-8372 IS - 780 ER - TY - THES A1 - Numberger, Daniela T1 - Urban wastewater and lakes as habitats for bacteria and potential vectors for pathogens T1 - Urbane Abwässer und Seen als Habitat für Bakterien und potentielle Vektoren für Krankheitserreger N2 - Wasser ist lebensnotwendig und somit eine essentielle Ressource. Jedoch sind unsere Süßwasser-Ressourcen begrenzt und ihre Erhaltung daher besonders wichtig. Verschmutzungen mit Chemikalien und Krankheitserregern, die mit einer wachsenden Bevölkerung und Urbanisierung einhergehen, verschlechtern die Qualität unseres Süßwassers. Außerdem kann Wasser als Übertragungsvektor für Krankheitserreger dienen und daher wasserbürtige Krankheiten verursachen. Der Leibniz-Forschungsverbund INFECTIONS‘21 untersuchte innerhalb der interdisziplinären Forschungsgruppe III - „Wasser", Gewässer als zentralen Mittelpunkt für Krankheiterreger. Dabei konzentrierte man sich auf Clostridioides difficile sowie aviäre Influenza A-Viren, von denen angenommen wird, dass sie in die Gewässer ausgeschieden werden. Ein weiteres Ziel bestand darin, die bakterielle Gemeinschaften eines Klärwerkes der deutschen Hauptstadt Berlin zu charakterisieren, um anschließend eine Bewertung des potentiellen Gesundheitsrisikos geben zu können. Bakterielle Gemeinschaften des Roh- und Klarwassers aus dem Klärwerk unterschieden sich signifikant voneinander. Der Anteil an Darm-/Fäkalbakterien war relativ niedrig und potentielle Darmpathogene wurden größtenteils aus dem Rohwasser entfernt. Ein potentielles Gesundheitsrisiko konnte allerdings von potentiell pathogenen Legionellen wie L. lytica festgestellt werden, deren relative Abundanz im Klarwasser höher war als im Rohwasser. Es wurden außerdem drei C. difficile-Isolate aus den Klärwerk-Rohwasser und einem städtischen Badesee in Berlin (Weisser See) gewonnen und sequenziert. Die beiden Isolate aus dem Klärwerk tragen keine Toxin-Gene, wohingegen das Isolat aus dem See Toxin-Gene besitzt. Alle drei Isolate sind sehr nah mit humanen Stämmen verwandt. Dies deutet auf ein potentielles, wenn auch sporadisches Gesundheitsrisiko hin. (Aviäre) Influenza A-Viren wurden in 38.8% der untersuchten Sedimentproben mittels PCR detektiert, aber die Virusisolierung schlug fehl. Ein Experiment mit beimpften Wasser- und Sedimentproben zeigte, dass für die Isolierung aus Sedimentproben eine relativ hohe Viruskonzentration nötig ist. In Wasserproben ist jedoch ein niedriger Titer an Influenza A-Viren ausreichend, um eine Infektion auszulösen. Es konnte zudem auch festgestellt werden, dass sich „Madin-Darby Canine Kidney (MDCK)―-Zellkulturen im Gegensatz zu embryonierten Hühnereiern besser eignen, um Influenza A-Viren aus Sediment zu isolieren. Zusammenfassend lässt sich sagen, dass diese Arbeit mögliche Gesundheitsrisiken aufgedeckt hat, wie etwa durch Legionellen im untersuchten Berliner Klärwerk, deren relative Abundanz in geklärtem Abwasser höher ist als im Rohwasser. Desweiteren wird indiziert, dass Abwasser und Gewässer als Reservoir und Vektor für pathogene Organismen dienen können, selbst für nicht-typische Wasser-Pathogene wie C. difficile. N2 - Water is essential to life and thus, an essential resource. However, freshwater resources are limited and their maintenance is crucial. Pollution with chemicals and pathogens through urbanization and a growing population impair the quality of freshwater. Furthermore, water can serve as vector for the transmission of pathogens resulting in water-borne illness. The Interdisciplinary Research Group III – "Water" of the Leibniz alliance project INFECTIONS‘21 investigated water as a hub for pathogens focusing on Clostridioides difficile and avian influenza A viruses that may be shed into the water. Another aim of this study was to characterize the bacterial communities in a wastewater treatment plant (WWTP) of the capital Berlin, Germany to further assess potential health risks associated with wastewater management practices. Bacterial communities of WWTP inflow and effluent differed significantly. The proportion of fecal/enteric bacteria was relatively low and OTUs related to potential enteric pathogens were largely removed from inflow to effluent. However, a health risk might exist as an increased relative abundance of potential pathogenic Legionella spp. such as L. lytica was observed. Three Clostridioides difficile isolates from wastewater inflow and an urban bathing lake in Berlin (‗Weisser See‘) were obtained and sequenced. The two isolates from the wastewater did not carry toxin genes, whereas the isolate from the lake was positive for the toxin genes. All three isolates were closely related to human strains. This indicates a potential, but rather sporadic health risk. Avian influenza A viruses were detected in 38.8% of sediment samples by PCR, but virus isolation failed. An experiment with inoculated freshwater and sediment samples showed that virus isolation from sediment requires relatively high virus concentrations and worked much better in Madin-Darby Canine Kidney (MDCK) cell cultures than in embryonated chicken eggs, but low titre of influenza contamination in freshwater samples was sufficient to recover virus. In conclusion, this work revealed potential health risks coming from bacterial groups with pathogenic potential such as Legionella spp. whose relative abundance is higher in the released effluent than in the inflow of the investigated WWTP. It further indicates that water bodies such as wastewater and lake sediments can serve as reservoir and vector, even for non-typical water-borne or water-transmitted pathogens such as C. difficile. KW - water KW - Wasser KW - bacteria KW - Bakterien KW - influenza A viruses KW - Influenza A Viren KW - pathogens KW - Krankheitserreger Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-437095 ER -