Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Abteilungen OPUS4-47918 Dissertation Wenk, Sebastian Engineering formatotrophic growth in Escherichia coli To meet the demands of a growing world population while reducing carbon dioxide (CO2) emissions, it is necessary to capture CO2 and convert it into value-added compounds. In recent years, metabolic engineering of microbes has gained strong momentum as a strategy for the production of valuable chemicals. As common microbial feedstocks like glucose directly compete with human consumption, the one carbon (C1) compound formate was suggested as an alternative feedstock. Formate can be easily produced by various means including electrochemical reduction of CO2 and could serve as a feedstock for microbial production, hence presenting a novel entry point for CO2 to the biosphere and a storage option for excess electricity. Compared to the gaseous molecule CO2, formate is a highly soluble compound that can be easily handled and stored. It can serve as a carbon and energy source for natural formatotrophs, but these microbes are difficult to cultivate and engineer. In this work, I present the results of several projects that aim to establish efficient formatotrophic growth of E. coli - which cannot naturally grow on formate - via synthetic formate assimilation pathways. In the first study, I establish a workflow for growth-coupled metabolic engineering of E. coli. I demonstrate this approach by presenting an engineering scheme for the PFL-threonine cycle, a synthetic pathway for anaerobic formate assimilation in E. coli. The described methods are intended to create a standardized toolbox for engineers that aim to establish novel metabolic routes in E. coli and related organisms. The second chapter presents a study on the catalytic efficiency of C1-oxidizing enzymes in vivo. As formatotrophic growth requires generation of both energy and biomass from formate, the engineered E. coli strains need to be equipped with a highly efficient formate dehydrogenase, which provides reduction equivalents and ATP for formate assimilation. I engineered a strain that cannot generate reducing power and energy for cellular growth, when fed on acetate. Under this condition, the strain depends on the introduction of an enzymatic system for NADH regeneration, which could further produce ATP via oxidative phosphorylation. I show that the strain presents a valuable testing platform for C1-oxidizing enzymes by testing different NAD-dependent formate and methanol dehydrogenases in the energy auxotroph strain. Using this platform, several candidate enzymes with high in vivo activity, were identified and characterized as potential energy-generating systems for synthetic formatotrophic or methylotrophic growth in E. coli.   In the third chapter, I present the establishment of the serine threonine cycle (STC) - a synthetic formate assimilation pathway - in E. coli. In this pathway, formate is assimilated via formate tetrahydrofolate ligase (FtfL) from Methylobacterium extorquens (M. extorquens). The carbon from formate is attached to glycine to produce serine, which is converted into pyruvate entering central metabolism. Via the natural threonine synthesis and cleavage route, glycine is regenerated and acetyl-CoA is produced as the pathway product. I engineered several selection strains that depend on different STC modules for growth and determined key enzymes that enable high flux through threonine synthesis and cleavage. I could show that expression of an auxiliary formate dehydrogenase was required to achieve growth via threonine synthesis and cleavage on pyruvate. By overexpressing most of the pathway enzymes from the genome, and applying adaptive laboratory evolution, growth on glycine and formate was achieved, indicating the activity of the complete cycle. The fourth chapter shows the establishment of the reductive glycine pathway (rGP) - a short, linear formate assimilation route - in E. coli. As in the STC, formate is assimilated via M. extorquens FtfL. The C1 from formate is condensed with CO2 via the reverse reaction of the glycine cleavage system to produce glycine. Another carbon from formate is attached to glycine to form serine, which is assimilated into central metabolism via pyruvate. The engineered E. coli strain, expressing most of the pathway genes from the genome, can grow via the rGP with formate or methanol as a sole carbon and energy source. 2020 V, 107 Institut für Biochemie und Biologie OPUS4-47019 Dissertation Schimpf, Stefan Herkunft und Ablagerungsmilieu quartärer Sedimente im Einzugsgebiet des Heihe, NW China Der zentralasiatische Naturraum, wie er sich uns heute präsentiert, ist das Ergebnis eines Zusammenwirkens vieler verschiedener Faktoren über Jahrmillionen hinweg. Im aktuellen Kontext des Klimawandels zeigt sich jedoch, wie stark sich Stoffflüsse auch kurzfristig ändern und dabei das Gesicht der Landschaft verwandeln können. Die Gobi-Wüste in der Inneren Mongolei (China), als Teil der gleichnamigen Trockenregionen Nordwestchinas, ist aufgrund der Ausgestaltung ihrer landschaftsprägenden Elemente sowie ihrer Landschaftsdynamik, im Zusammenhang mit der Lage zum Tibet-Plateau, in den Fokus der klimageschichtlichen Grundlagenforschung gerückt. Als großes Langzeitarchiv unterschiedlichster fluvialer, lakustriner und äolischer Sedimente stellt sie eine bedeutende Lokalität zur Rekonstruktion von lokalen und regionalen Stoffflüssen dar.. Andererseits ist die Gobi-Wüste zugleich auch eine bedeutende Quelle für den überregionalen Staubtransport, da sie aufgrund der klimatischen Bedingungen insbesondere der Erosion durch Ausblasung preisgegeben wird. Vor diesem Hintergrund erfolgten zwischen 2011 und 2014, im Rahmen des BMBF-Verbundprogramms WTZ Zentralasien - Monsundynamik & Geoökosysteme (Förderkennzeichen 03G0814), mehrere deutsch-chinesische Expeditionen in das Ejina-Becken (Innere Mongolei) und das Qilian Shan-Vorland. Im Zuge dieser Expeditionen wurden für eine Bestimmung potenzieller Sedimentquellen erstmals zahlreiche Oberflächenproben aus dem gesamten Einzugsgebiet des Heihe (schwarzer Fluss) gesammelt. Zudem wurden mit zwei Bohrungen im inneren des Ejina-Beckens, ergänzende Sedimentbohrkerne zum bestehenden Bohrkern D100 (siehe Wünnemann (2005)) abgeteuft, um weit reichende, ergänzende Informationen zur Landschaftsgeschichte und zum überregionalen Sedimenttransfer zu erhalten. Gegenstand und Ziel der vorliegenden Doktorarbeit ist die sedimentologisch-mineralogische Charakterisierung des Untersuchungsgebietes in Bezug auf potenzielle Sedimentquellen und Stoffflüsse des Ejina-Beckens sowie die Rekonstruktion der Ablagerungsgeschichte eines dort erbohrten, 19m langen Sedimentbohrkerns (GN100). Schwerpunkt ist hierbei die Klärung der Sedimentherkunft innerhalb des Bohrkerns sowie die Ausweisung von Herkunftssignalen und möglichen Sedimentquellen bzw. Sedimenttransportpfaden. Die methodische Herangehensweise basiert auf einem Multi-Proxy-Ansatz zur Charakterisierung der klastischen Sedimentfazies anhand von Geländebeobachtungen, lithologisch-granulometrischen und mineralogisch-geochemischen Analysen sowie statistischen Verfahren. Für die mineralogischen Untersuchungen der Sedimente wurde eine neue, rasterelektronenmikroskopische Methode zur automatisierten Partikelanalyse genutzt und den traditionellen Methoden gegenübergestellt. Die synoptische Betrachtung der granulometrischen, geochemischen und mineralogischen Befunde der Oberflächensedimente ergibt für das Untersuchungsgebiet ein logisches Kaskadenmodell mit immer wiederkehrenden Prozessbereichen und ähnlichen Prozesssignalen. Die umfangreichen granulometrischen Analysen deuten dabei auf abnehmende Korngrößen mit zunehmender Entfernung vom Qilian Shan hin und ermöglichen die Identifizierung von vier texturellen Signalen: den fluvialen Sanden, den Dünensanden, den Stillwassersedimenten und Stäuben. Diese Ergebnisse können als Interpretationsgrundlage für die Korngrößenanalysen des Bohrkerns genutzt werden. Somit ist es möglich, die Ablagerungsgeschichte der Bohrkernsedimente zu rekonstruieren und in Verbindung mit eigenen und literaturbasierten Datierungen in einen Gesamtkontext einzuhängen. Für das Untersuchungsgebiet werden somit vier Ablagerungsphasen ausgewiesen, die bis in die Zeit des letzten glazialen Maximums (LGM) zurückreichen. Während dieser Ablagerungsphasen kam es im Zuge unterschiedlicher Aktivitäts- und Stabilitätsphasen zu einer kontinuierlichen Progradation und Überprägung des Schwemmfächers. Eine besonders aktive Phase kann zwischen 8 ka und 4 ka BP festgestellt werden, während der es aufgrund zunehmender fluvialer Aktivitäten zu einer deutlich verstärkten Schwemmfächerdynamik gekommen zu sein scheint. In den Abschnitten davor und danach waren es vor allem äolische Prozesse, die zu einer Überprägung des Schwemmfächers geführt haben. Hinsichtlich der mineralogischen Herkunftssignale gibt es eine große Variabilität. Dies spiegelt die enorme Heterogenität der Geologie des Untersuchungsgebietes wider, wodurch die räumlichen Signale nicht sehr stark ausgeprägt sind. Dennoch, können für das Einzugsgebiet drei größere Bereiche deklariert werden, die als Herkunftsgebiet in Frage kommen. Das östliche Qilian Shan Vorland zeichnet sich dabei durch deutlich höhere Chloritgehalte als primäre Quelle für die Sedimente im Ejina-Becken aus. Sie unterscheiden sich insbesondere durch stark divergierende Chloritgehalte in der Tonmineral- und Gesamtmineralfraktion, was das östliche Qilian Shan Vorland als primäre Quelle für die Sedimente im Ejina-Becken auszeichnet. Dies steht in Zusammenhang mit den Grünschiefern, Ophioliten und Serpentiniten in diesem Bereich. Geochemisch deutet vor allem das Cr/Rb-Verhältnis eine große Variabilität innerhalb des Einzugsgebietes an. Auch hier ist es das östliche Vorland, welches aufgrund seines hohen Anteils an mafischen Gesteinen reich an Chromiten und Spinellen ist und sich somit vom restlichen Untersuchungsgebiet abhebt. Die zeitliche aber auch die generelle Variabilität der Sedimentherkunft lässt sich in den Bohrkernsedimenten nicht so deutlich nachzeichnen. Die mineralogisch-sedimentologischen Eigenschaften der erbohrten klastischen Sedimente zeugen zwar von zwischenzeitlichen Änderungen bei der Sedimentherkunft, diese sind jedoch nicht so deutlich ausgeprägt, wie es die Quellsignale in den Oberflächensedimenten vermuten lassen. Ein Grund dafür scheint die starke Vermischung unterschiedlichster Sedimente während des Transportes zu sein. Die Kombination der Korngrößenergebnisse mit den Befunden der Gesamt- und Schwermineralogie deuten darauf hin, dass es zwischenzeitlich eine Phase mit überwiegend äolischen Prozessen gegeben hat, die mit einem Sedimenteintrag aus dem westlichen Bei Shan in Verbindung stehen. Neben der Zunahme ultrastabiler Schwerminerale wie Zirkon und Granat und der Abnahme opaker Schwerminerale, weisen vor allem die heutigen Verhältnisse darauf hin. Der Vergleich der traditionellen Schwermineralanalyse mit der Computer-Controlled-Scanning-Electron-Microscopy (kurz: CCSEM), die eine automatisierte Partikelauswertung der Proben ermöglicht, zeigt den deutlichen Vorteil der modernen Analysemethode. Neben einem zeitlichen Vorteil, den man durch die automatisierte Abarbeitung der vorbereiteten Proben erlangen kann, steht vor allem die deutlich größere statistische Signifikanz des Ergebnisses im Vordergrund. Zudem können mit dieser Methode auch chemische Varietäten einiger Schwerminerale bestimmt werden, die eine noch feinere Klassifizierung und sicherere Aussagen zu einer möglichen Sedimentherkunft ermöglichen. Damit ergeben sich außerdem verbesserte Aussagen zu Zusammensetzungen und Entstehungsprozessen der abgelagerten Sedimente. Die Studie verdeutlicht, dass die Sedimentherkunft innerhalb des Untersuchungsgebietes sowie die ablaufenden Prozesse zum Teil stark von lokalen Gegebenheiten abhängen. Die Heterogenität der Geologie und die Größe des Einzugsgebietes sowie die daraus resultierende Komplexität der Sedimentgenese, machen exakte Zuordnungen zu klar definierten Sedimentquellen sehr schwer. Dennoch zeigen die Ergebnisse, dass die Sedimentzufuhr in das Ejina-Becken in erster Linie durch fluviale klastische Sedimente des Heihe aus dem Qilian Shan erfolgt sein muss. Die Untersuchungsergebnisse zeigen jedoch ebenso die Notwendigkeit einer ergänzenden Bearbeitung angrenzender Untersuchungsgebiete, wie beispielsweise den Gobi-Altai im Norden oder den Beishan im Westen, sowie die Verdichtung der Oberflächenbeprobung zur feineren Auflösung von lokalen Sedimentquellen. 2020 xi, 186 Institut für Geowissenschaften OPUS4-57286 Wissenschaftlicher Artikel Tofelde, Stefanie; Bufe, Aaron; Turowski, Jens M. Hillslope Sediment Supply Limits Alluvial Valley Width River-valley morphology preserves information on tectonic and climatic conditions that shape landscapes. Observations suggest that river discharge and valley-wall lithology are the main controls on valley width. Yet, current models based on these observations fail to explain the full range of cross-sectional valley shapes in nature, suggesting hitherto unquantified controls on valley width. In particular, current models cannot explain the existence of paired terrace sequences that form under cyclic climate forcing. Paired river terraces are staircases of abandoned floodplains on both valley sides, and hence preserve past valley widths. Their formation requires alternating phases of predominantly river incision and predominantly lateral planation, plus progressive valley narrowing. While cyclic Quaternary climate changes can explain shifts between incision and lateral erosion, the driving mechanism of valley narrowing is unknown. Here, we extract valley geometries from climatically formed, alluvial river-terrace sequences and show that across our dataset, the total cumulative terrace height (here: total valley height) explains 90%-99% of the variance in valley width at the terrace sites. This finding suggests that valley height, or a parameter that scales linearly with valley height, controls valley width in addition to river discharge and lithology. To explain this valley-width-height relationship, we reformulate existing valley-width models and suggest that, when adjusting to new boundary conditions, alluvial valleys evolve to a width at which sediment removal from valley walls matches lateral sediment supply from hillslope erosion. Such a hillslope-channel coupling is not captured in current valley-evolution models. Our model can explain the existence of paired terrace sequences under cyclic climate forcing and relates valley width to measurable field parameters. Therefore, it facilitates the reconstruction of past climatic and tectonic conditions from valley topography. Hoboken, New Jersey, USA American Geophysical Union (AGU); Wiley 2022 20 AGU Advances 10.1029/2021AV000641 Institut für Geowissenschaften OPUS4-57831 Wissenschaftlicher Artikel Miklashevsky, Alex; Fischer, Martin H.; Lindemann, Oliver Spatial-numerical associations without a motor response? Grip force says 'Yes' In numerical processing, the functional role of Spatial-Numerical Associations (SNAs, such as the association of smaller numbers with left space and larger numbers with right space, the Mental Number Line hypothesis) is debated. Most studies demonstrate SNAs with lateralized responses, and there is little evidence that SNAs appear when no response is required. We recorded passive holding grip forces in no-go trials during number processing. In Experiment 1, participants performed a surface numerical decision task ("Is it a number or a letter?"). In Experiment 2, we used a deeper semantic task ("Is this number larger or smaller than five?"). Despite instruction to keep their grip force constant, participants' spontaneous grip force changed in both experiments: Smaller numbers led to larger force increase in the left than in the right hand in the numerical decision task (500-700 ms after stimulus onset). In the semantic task, smaller numbers again led to larger force increase in the left hand, and larger numbers increased the right-hand holding force. This effect appeared earlier (180 ms) and lasted longer (until 580 ms after stimulus onset). This is the first demonstration of SNAs with passive holding force. Our result suggests that (1) explicit motor response is not a prerequisite for SNAs to appear, and (2) the timing and strength of SNAs are task-dependent. (216 words). Amsterdam Elsevier 2022 17 Acta Psychologica 231 1 17 10.1016/j.actpsy.2022.103791 Extern OPUS4-57675 Arbeitspapier Kienbaum, Janna; Fischer, Patryk; Passmann, Sven Forschungsdatenmanagement bei personenbezogenen Daten Das Dokument "Forschungsdatenmanagement bei personenbezogenen Daten - eine Handreichung" versammelt zentrale Inhalte, Verweise und Vorgehensweisen für Forscher*innen, die in einer Studie personenbezogene Daten erheben und diese verarbeiten, archivieren oder veröffentlichen wollen. Die Handreichung verweist an den entsprechenden Abschnitten auf weiterführende Materialien wie insbesondere die Handreichung „Datenschutz" des Rats für die Sozial-, Verhaltens-, Bildungs- und Wirtschaftswissenschaften (RatSWD). 2023 18 10.5281/zenodo.7428524 Extern OPUS4-57673 Arbeitspapier Kienbaum, Janna; Würfl, Katja; Favella, Gianpiero Leitfaden für einen qualitativen Methoden- und Feldbericht Der Leitfaden für einen qualitativen Methoden- und Feldbericht ist im Rahmen des DFG-Verbundprojektes "FDNext" (2020-2023) entstanden und dient als Orientierungshilfe für die Dokumentation erhobener Daten mit dem Schwerpunkt auf die qualitative Bildungsforschung. 2022 12 10.5281/zenodo.7247993 Extern OPUS4-58128 Wissenschaftlicher Artikel Lindner, Nadja; Moeller, Korbinian; Hildebrandt, Frauke; Hasselhorn, Marcus; Lonnemann, Jan Children's use of egocentric reference frames in spatial language is related to their numerical magnitude understanding Numerical magnitude information is assumed to be spatially represented in the form of a mental number line defined with respect to a body-centred, egocentric frame of reference. In this context, spatial language skills such as mastery of verbal descriptions of spatial position (e.g., in front of, behind, to the right/left) have been proposed to be relevant for grasping spatial relations between numerical magnitudes on the mental number line. We examined 4- to 5-year-old's spatial language skills in tasks that allow responses in egocentric and allocentric frames of reference, as well as their relative understanding of numerical magnitude (assessed by a number word comparison task). In addition, we evaluated influences of children's absolute understanding of numerical magnitude assessed by their number word comprehension (montring different numbers using their fingers) and of their knowledge on numerical sequences (determining predecessors and successors as well as identifying missing dice patterns of a series). Results indicated that when considering responses that corresponded to the egocentric perspective, children's spatial language was associated significantly with their relative numerical magnitude understanding, even after controlling for covariates, such as children's SES, mental rotation skills, and also absolute magnitude understanding or knowledge on numerical sequences. This suggests that the use of egocentric reference frames in spatial language may facilitate spatial representation of numbers along a mental number line and thus seem important for preschoolers' relative understanding of numerical magnitude. Lausanne, Schweiz Frontiers 2022 13 Frontiers in Psychology 1 13 10.3389/fpsyg.2022.943191 Extern OPUS4-58100 Wissenschaftlicher Artikel Hecker, Pascal; Steckhan, Nico; Eyben, Florian; Schuller, Björn Wolfgang; Arnrich, Bert Voice Analysis for Neurological Disorder Recognition - A Systematic Review and Perspective on Emerging Trends Quantifying neurological disorders from voice is a rapidly growing field of research and holds promise for unobtrusive and large-scale disorder monitoring. The data recording setup and data analysis pipelines are both crucial aspects to effectively obtain relevant information from participants. Therefore, we performed a systematic review to provide a high-level overview of practices across various neurological disorders and highlight emerging trends. PRISMA-based literature searches were conducted through PubMed, Web of Science, and IEEE Xplore to identify publications in which original (i.e., newly recorded) datasets were collected. Disorders of interest were psychiatric as well as neurodegenerative disorders, such as bipolar disorder, depression, and stress, as well as amyotrophic lateral sclerosis amyotrophic lateral sclerosis, Alzheimer's, and Parkinson's disease, and speech impairments (aphasia, dysarthria, and dysphonia). Of the 43 retrieved studies, Parkinson's disease is represented most prominently with 19 discovered datasets. Free speech and read speech tasks are most commonly used across disorders. Besides popular feature extraction toolkits, many studies utilise custom-built feature sets. Correlations of acoustic features with psychiatric and neurodegenerative disorders are presented. In terms of analysis, statistical analysis for significance of individual features is commonly used, as well as predictive modeling approaches, especially with support vector machines and a small number of artificial neural networks. An emerging trend and recommendation for future studies is to collect data in everyday life to facilitate longitudinal data collection and to capture the behavior of participants more naturally. Another emerging trend is to record additional modalities to voice, which can potentially increase analytical performance. Lausanne, Schweiz Frontiers Media SA 2022 16 Frontiers in Digital Health 10.3389/fdgth.2022.842301 Extern OPUS4-7032 Konferenzveröffentlichung Curzon, Paul; Kalas, Ivan; Schubert, Sigrid; Schaper, Niclas; Barnes, Jan; Kennewell, Steve; Bröker, Kathrin; Kastens, Uwe; Magenheim, Johannes; Dagiene, Valentina; Stupuriene, Gabriele; Ellis, Jason Brent; Abreu-Ellis, Carla Reis; Grillenberger, Andreas; Romeike, Ralf; Haugsbakken, Halvdan; Jones, Anthony; Lewin, Cathy; McNicol, Sarah; Nelles, Wolfgang; Neugebauer, Jonas; Ohrndorf, Laura; Schaper, Niclas; Schubert, Sigrid; Opel, Simone; Kramer, Matthias; Trommen, Michael; Pottbäcker, Florian; Ilaghef, Youssef; Passig, David; Tzuriel, David; Kedmi, Ganit Eshel; Saito, Toshinori; Webb, Mary; Weigend, Michael; Bottino, Rosa; Chioccariello, Augusto; Christensen, Rhonda; Knezek, Gerald; Gioko, Anthony Maina; Angondi, Enos Kiforo; Waga, Rosemary; Ohrndorf, Laura; Or-Bach, Rachel; Preston, Christina; Younie, Sarah; Przybylla, Mareen; Romeike, Ralf; Reynolds, Nicholas; Swainston, Andrew; Bendrups, Faye; Sysło, Maciej M.; Kwiatkowska, Anna Beata; Zieris, Holger; Gerstberger, Herbert; Müller, Wolfgang; Büchner, Steffen; Opel, Simone; Schiller, Thomas; Wegner, Christian; Zender, Raphael; Lucke, Ulrike; Diethelm, Ira; Syrbe, Jörn; Lai, Kwok-Wing; Davis, Niki; Eickelmann, Birgit; Erstad, Ola; Fisser, Petra; Gibson, David; Khaddage, Ferial; Knezek, Gerald; Micheuz, Peter; Kloos, Carlos Delgado Brinda, Torsten; Reynolds, Nicholas; Romeike, Ralf; Schwill, Andreas KEYCIT 2014 In our rapidly changing world it is increasingly important not only to be an expert in a chosen field of study but also to be able to respond to developments, master new approaches to solving problems, and fulfil changing requirements in the modern world and in the job market. In response to these needs key competencies in understanding, developing and using new digital technologies are being brought into focus in school and university programmes. The IFIP TC3 conference "KEYCIT - Key Competences in Informatics and ICT (KEYCIT 2014)" was held at the University of Potsdam in Germany from July 1st to 4th, 2014 and addressed the combination of key competencies, Informatics and ICT in detail. The conference was organized into strands focusing on secondary education, university education and teacher education (organized by IFIP WGs 3.1 and 3.3) and provided a forum to present and to discuss research, case studies, positions, and national perspectives in this field. Potsdam Universitätsverlag Potsdam 2015 438 978-3-86956-292-6 urn:nbn:de:kobv:517-opus4-70325 Institut für Informatik und Computational Science OPUS4-57959 Wissenschaftlicher Artikel Ogunkola, Moses Olalekan; Guiraudie-Capraz, Gaelle; Féron, François; Leimkühler, Silke The Human Mercaptopyruvate Sulfurtransferase TUM1 Is Involved in Moco Biosynthesis, Cytosolic tRNA Thiolation and Cellular Bioenergetics in Human Embryonic Kidney Cells Sulfur is an important element that is incorporated into many biomolecules in humans. The incorporation and transfer of sulfur into biomolecules is, however, facilitated by a series of different sulfurtransferases. Among these sulfurtransferases is the human mercaptopyruvate sulfurtransferase (MPST) also designated as tRNA thiouridine modification protein (TUM1). The role of the human TUM1 protein has been suggested in a wide range of physiological processes in the cell among which are but not limited to involvement in Molybdenum cofactor (Moco) biosynthesis, cytosolic tRNA thiolation and generation of H2S as signaling molecule both in mitochondria and the cytosol. Previous interaction studies showed that TUM1 interacts with the L-cysteine desulfurase NFS1 and the Molybdenum cofactor biosynthesis protein 3 (MOCS3). Here, we show the roles of TUM1 in human cells using CRISPR/Cas9 genetically modified Human Embryonic Kidney cells. Here, we show that TUM1 is involved in the sulfur transfer for Molybdenum cofactor synthesis and tRNA thiomodification by spectrophotometric measurement of the activity of sulfite oxidase and liquid chromatography quantification of the level of sulfur-modified tRNA. Further, we show that TUM1 has a role in hydrogen sulfide production and cellular bioenergetics. 1 Basel, Schweiz MDPI 2023 23 Biomolecules 13 1 23 10.3390/biom13010144 Institut für Biochemie und Biologie OPUS4-57835 Wissenschaftlicher Artikel Göldel, Julia M.; Kamrath, Clemens; Minden, Kirsten; Wiegand, Susanna; Lanzinger, Stefanie; Sengler, Claudia; Weihrauch-Blüher, Susann; Holl, Reinhard W.; Tittel, Sascha René; Warschburger, Petra Access to Healthcare for Children and Adolescents with a Chronic Health Condition during the COVID-19 Pandemic: First Results from the KICK-COVID Study in Germany This study examines the access to healthcare for children and adolescents with three common chronic diseases (type-1 diabetes (T1D), obesity, or juvenile idiopathic arthritis (JIA)) within the 4th (Delta), 5th (Omicron), and beginning of the 6th (Omicron) wave (June 2021 until July 2022) of the COVID-19 pandemic in Germany in a cross-sectional study using three national patient registries. A paper-and-pencil questionnaire was given to parents of pediatric patients (<21 years) during the routine check-ups. The questionnaire contains self-constructed items assessing the frequency of healthcare appointments and cancellations, remote healthcare, and satisfaction with healthcare. In total, 905 parents participated in the T1D-sample, 175 in the obesity-sample, and 786 in the JIA-sample. In general, satisfaction with healthcare (scale: 0-10; 10 reflecting the highest satisfaction) was quite high (median values: T1D 10, JIA 10, obesity 8.5). The proportion of children and adolescents with canceled appointments was relatively small (T1D 14.1%, JIA 11.1%, obesity 20%), with a median of 1 missed appointment, respectively. Only a few parents (T1D 8.6%; obesity 13.1%; JIA 5%) reported obstacles regarding health services during the pandemic. To conclude, it seems that access to healthcare was largely preserved for children and adolescents with chronic health conditions during the COVID-19 pandemic in Germany. 1 Basel, Schweiz MDPI 2022 11 Children 10 1 11 10.3390/children10010010 Extern OPUS4-57956 Wissenschaftlicher Artikel Marggraf, Lara Christin; Lindecke, Oliver; Voigt, Christian C.; Pētersons, Gunārs; Voigt-Heucke, Silke Luise Nathusius' bats, Pipistrellus nathusii, bypass mating opportunities of their own species, but respond to foraging heterospecifics on migratory transit flights In late summer, migratory bats of the temperate zone face the challenge of accomplishing two energy-demanding tasks almost at the same time: migration and mating. Both require information and involve search efforts, such as localizing prey or finding potential mates. In non-migrating bat species, playback studies showed that listening to vocalizations of other bats, both con-and heterospecifics, may help a recipient bat to find foraging patches and mating sites. However, we are still unaware of the degree to which migrating bats depend on con-or heterospecific vocalizations for identifying potential feeding or mating opportunities during nightly transit flights. Here, we investigated the vocal responses of Nathusius' pipistrelle bats, Pipistrellus nathusii, to simulated feeding and courtship aggregations at a coastal migration corridor. We presented migrating bats either feeding buzzes or courtship calls of their own or a heterospecific migratory species, the common noctule, Nyctalus noctula. We expected that during migratory transit flights, simulated feeding opportunities would be particularly attractive to bats, as well as simulated mating opportunities which may indicate suitable roosts for a stopover. However, we found that when compared to the natural silence of both pre-and post-playback phases, bats called indifferently during the playback of conspecific feeding sounds, whereas P. nathusii echolocation call activity increased during simulated feeding of N. noctula. In contrast, the call activity of P. nathusii decreased during the playback of conspecific courtship calls, while no response could be detected when heterospecific call types were broadcasted. Our results suggest that while on migratory transits, P. nathusii circumnavigate conspecific mating aggregations, possibly to save time or to reduce the risks associated with social interactions where aggression due to territoriality might be expected. This avoidance behavior could be a result of optimization strategies by P. nathusii when performing long-distance migratory flights, and it could also explain the lack of a response to simulated conspecific feeding. However, the observed increase of activity in response to simulated feeding of N. noctula, suggests that P. nathusii individuals may be eavesdropping on other aerial hawking insectivorous species during migration, especially if these occupy a slightly different foraging niche. Lausanne, Schweiz Frontiers 2023 10 Frontiers in Ecology and Evolution 1 10 10.3389/fevo.2022.908560 Institut für Biochemie und Biologie OPUS4-2942 Wissenschaftlicher Artikel Dörfler, Thomas Geographie und Dekonstruktion Inhalt: - Der Ort - Das Symptom - Der Jargon des Vermeintlichen, oder: vermeintliche Philosophie - Was ist: Dekonstruktion? - Zur Genese - Dekonstruktion und Geographie - Grenz-Philosophie - Die Welt als Text - Versuch über die Grenze, in geographischer Absicht 2005 18 Geographische Revue : Zeitschrift für Literatur und Diskussion 7 1/2 67 85 urn:nbn:de:kobv:517-opus-31098 Extern OPUS4-58229 Wissenschaftlicher Artikel Kühne, Franziska; Fauth, Henriette; Destina Sevde, Ay-Bryson; Visser, Leonie N.C.; Weck, Florian Communicating the diagnosis of cancer or depression: Results of a randomized controlled online study using video vignettes Background Communicating a diagnosis is highly important, yet complex, especially in the context of cancer and mental disorders. The aim was to explore the communication style of an oncologist vs. psychotherapist in an online study. Methods Patients (N = 136: 65 cancer, 71 depression) were randomly assigned to watch a standardized video vignette with one of two communication styles (empathic vs. unempathic). Outcome measures of affectivity, information recall, communication skills, empathy and trust were applied. Results Regardless of diagnosis, empathic communication was associated with the perception of a significantly more empathic (p < 0.001, η2partial = 0.08) and trustworthy practitioner (p = 0.014, η2partial = 0.04) with better communication skills (p = 0.013, η2partial = 0.05). Cancer patients reported a larger decrease in positive affect (p < 0.001, η2partial = 0.15) and a larger increase in negative affect (p < 0.001, η2partial = 0.14) from pre- to post-video than depressive patients. Highly relevant information was recalled better in both groups (p < 0.001, d = 0.61-1.06). Conclusions The results highlight the importance of empathy while communicating both a diagnosis of cancer and a mental disorder. Further research should focus on the communication of a mental disorder in association with cancer. 24 Hoboken, New Jersey, USA Wiley 2021 10 Cancer Medicine 10 9012 9021 10.1002/cam4.4396 Extern OPUS4-1201 Buch (Monographie) Ceglarek, Petra; Wulsch, Iris; Frenzel, Tom; Ihle, Wolfgang Gesundheitsförderung in Brandenburger Kindertagesstätten : eine Bestandsaufnahme Theoretischer Hintergrund: Eine frühzeitige Gesundheitsförderung wird als wesentliches Bestimmungsstück zur Prävention körperlicher und psychischer Erkrankungen angesehen. Als ideales Setting für eine gezielte Gesundheitsförderung wird der Lebensraum Kita angesehen. Fragestellungen: Welche Aktivitäten zur Gesundheitsförderung gibt es bisher in den Brandenburger Kitas? Spielt hier die Bildungsnähe/-ferne der betreuten Kinder eine Rolle? In welchen Bereichen gibt es den größten Fort- und Weiterbildungsbedarf der Mitarbeiterinnen? Methode: Untersuchung einer Stichprobe von 269 Brandenburger Kitas mit insgesamt 21 653 betreuten Kindern. Erfassung struktureller Rahmenbedingungen, pädagogischer Konzepte, Aktivitäten zur Gesundheitsförderung, Partizipation und Fortbildungsbedarf. Ergebnisse: Es zeigte sich eine nachhaltige Umsetzung von Aktivitäten und große Kreativität. Dabei standen vor allem Angebote der Bereiche Bewegung und Ernährung im Zentrum. Psychosoziale Themen wurden seltener als Schwerpunkt benannt, wohingegen hierfür der größte Fortbildungbedarf (Ranking der Fortbildungsthemen: 1. Psychosoziale Entwicklung, 2. Psychische Probleme, 3. Stressbewältigung) vorlag. Potsdam Universitätsverlag Potsdam 2006 112 978-3-939469-50-6 urn:nbn:de:kobv:517-opus-13105 Extern OPUS4-57079 Wissenschaftlicher Artikel Reeg, Jette; Strigl, Lea; Jeltsch, Florian Agricultural buffer zone thresholds to safeguard functional bee diversity Wild bee species are important pollinators in agricultural landscapes. However, population decline was reported over the last decades and is still ongoing. While agricultural intensification is a major driver of the rapid loss of pollinating species, transition zones between arable fields and forest or grassland patches, i.e., agricultural buffer zones, are frequently mentioned as suitable mitigation measures to support wild bee populations and other pollinator species. Despite the reported general positive effect, it remains unclear which amount of buffer zones is needed to ensure a sustainable and permanent impact for enhancing bee diversity and abundance. To address this question at a pollinator community level, we implemented a process-based, spatially explicit simulation model of functional bee diversity dynamics in an agricultural landscape. More specifically, we introduced a variable amount of agricultural buffer zones (ABZs) at the transition of arable to grassland, or arable to forest patches to analyze the impact on bee functional diversity and functional richness. We focused our study on solitary bees in a typical agricultural area in the Northeast of Germany. Our results showed positive effects with at least 25% of virtually implemented agricultural buffer zones. However, higher amounts of ABZs of at least 75% should be considered to ensure a sufficient increase in Shannon diversity and decrease in quasi-extinction risks. These high amounts of ABZs represent effective conservation measures to safeguard the stability of pollination services provided by solitary bee species. As the model structure can be easily adapted to other mobile species in agricultural landscapes, our community approach offers the chance to compare the effectiveness of conservation measures also for other pollinator communities in future. 3 Hoboken, New Jersey, USA Wiley Online Library 2022 17 Ecology and Evolution 12 1 17 10.1002/ece3.8748 Institut für Biochemie und Biologie OPUS4-58133 Wissenschaftlicher Artikel Ziegler, Joceline; Pfitzner, Bjarne; Schulz, Heinrich; Saalbach, Axel; Arnrich, Bert Defending against Reconstruction Attacks through Differentially Private Federated Learning for Classification of Heterogeneous Chest X-ray Data Privacy regulations and the physical distribution of heterogeneous data are often primary concerns for the development of deep learning models in a medical context. This paper evaluates the feasibility of differentially private federated learning for chest X-ray classification as a defense against data privacy attacks. To the best of our knowledge, we are the first to directly compare the impact of differentially private training on two different neural network architectures, DenseNet121 and ResNet50. Extending the federated learning environments previously analyzed in terms of privacy, we simulated a heterogeneous and imbalanced federated setting by distributing images from the public CheXpert and Mendeley chest X-ray datasets unevenly among 36 clients. Both non-private baseline models achieved an area under the receiver operating characteristic curve (AUC) of 0.940.94 on the binary classification task of detecting the presence of a medical finding. We demonstrate that both model architectures are vulnerable to privacy violation by applying image reconstruction attacks to local model updates from individual clients. The attack was particularly successful during later training stages. To mitigate the risk of a privacy breach, we integrated Rényi differential privacy with a Gaussian noise mechanism into local model training. We evaluate model performance and attack vulnerability for privacy budgets ε∈{1,3,6,10}�∈{1,3,6,10}. The DenseNet121 achieved the best utility-privacy trade-off with an AUC of 0.940.94 for ε=6�=6. Model performance deteriorated slightly for individual clients compared to the non-private baseline. The ResNet50 only reached an AUC of 0.760.76 in the same privacy setting. Its performance was inferior to that of the DenseNet121 for all considered privacy constraints, suggesting that the DenseNet121 architecture is more robust to differentially private training. 14 Basel, Schweiz MDPI 2022 25 Sensors 22 10.3390/s22145195 Extern OPUS4-58178 Wissenschaftlicher Artikel Liebe, Thomas; Dordevic, Milos; Kaufmann, Jörn; Avetisyan, Araks; Skalej, Martin; Müller, Notger Germar Investigation of the functional pathogenesis of mild cognitive impairment by localisation-based locus coeruleus resting-state fMRI Dementia as one of the most prevalent diseases urges for a better understanding of the central mechanisms responsible for clinical symptoms, and necessitates improvement of actual diagnostic capabilities. The brainstem nucleus locus coeruleus (LC) is a promising target for early diagnosis because of its early structural alterations and its relationship to the functional disturbances in the patients. In this study, we applied our improved method of localisation-based LC resting-state fMRI to investigate the differences in central sensory signal processing when comparing functional connectivity (fc) of a patient group with mild cognitive impairment (MCI, n = 28) and an age-matched healthy control group (n = 29). MCI and control participants could be differentiated in their Mini-Mental-State-Examination (MMSE) scores (p < .001) and LC intensity ratio (p = .010). In the fMRI, LC fc to anterior cingulate cortex (FDR p < .001) and left anterior insula (FDR p = .012) was elevated, and LC fc to right temporoparietal junction (rTPJ, FDR p = .012) and posterior cingulate cortex (PCC, FDR p = .021) was decreased in the patient group. Importantly, LC to rTPJ connectivity was also positively correlated to MMSE scores in MCI patients (p = .017). Furthermore, we found a hyperactivation of the left-insula salience network in the MCI patients. Our results and our proposed disease model shed new light on the functional pathogenesis of MCI by directing to attentional network disturbances, which could aid new therapeutic strategies and provide a marker for diagnosis and prediction of disease progression. 18 New York, NY, USA Wiley 2022 13 Human Brain Mapping 43 5630 5642 10.1002/hbm.26039 Extern OPUS4-56169 Wissenschaftlicher Artikel Hauffe, Robert; Rath, Michaela; Agyapong, Wilson; Jonas, Wenke; Vogel, Heike; Schulz, Tim Julius; Schwarz, Maria; Kipp, Anna Patricia; Blüher, Matthias; Kleinridders, André Obesity Hinders the Protective Effect of Selenite Supplementation on Insulin Signaling The intake of high-fat diets (HFDs) containing large amounts of saturated long-chain fatty acids leads to obesity, oxidative stress, inflammation, and insulin resistance. The trace element selenium, as a crucial part of antioxidative selenoproteins, can protect against the development of diet-induced insulin resistance in white adipose tissue (WAT) by increasing glutathione peroxidase 3 (GPx3) and insulin receptor (IR) expression. Whether selenite (Se) can attenuate insulin resistance in established lipotoxic and obese conditions is unclear. We confirm that GPX3 mRNA expression in adipose tissue correlates with BMI in humans. Cultivating 3T3-L1 pre-adipocytes in palmitate-containing medium followed by Se treatment attenuates insulin resistance with enhanced GPx3 and IR expression and adipocyte differentiation. However, feeding obese mice a selenium-enriched high-fat diet (SRHFD) only resulted in a modest increase in overall selenoprotein gene expression in WAT in mice with unaltered body weight development, glucose tolerance, and insulin resistance. While Se supplementation improved adipocyte morphology, it did not alter WAT insulin sensitivity. However, mice fed a SRHFD exhibited increased insulin content in the pancreas. Overall, while selenite protects against palmitate-induced insulin resistance in vitro, obesity impedes the effect of selenite on insulin action and adipose tissue metabolism in vivo. 5 Basel, Schweiz MDPI 2022 16 Antioxidants 11 1 16 10.3390/antiox11050862 Institut für Ernährungswissenschaft OPUS4-53848 Wissenschaftlicher Artikel Wright, Michelle F.; Wachs, Sebastian; Huang, Zheng Adolescents' Popularity-Motivated Aggression and Prosocial Behaviors: The Roles of Callous-Unemotional Traits and Social Status Insecurity As competition over peer status becomes intense during adolescence, some adolescents develop insecure feelings regarding their social standing among their peers (i.e., social status insecurity). These adolescents sometimes use aggression to defend or promote their status. The aim of this study was to examine the relationships among social status insecurity, callous-unemotional (CU) traits, and popularity-motivated aggression and prosocial behaviors among adolescents, while controlling for gender. Another purpose was to examine the potential moderating role of CU traits in these relationships. Participants were 1,047 (49.2% girls; Mage = 12.44 years; age range from 11 to 14 years) in the 7th or 8th grades from a large Midwestern city. They completed questionnaires on social status insecurity, CU traits, and popularity-motivated relational aggression, physical aggression, cyberaggression, and prosocial behaviors. A structural regression model was conducted, with gender as a covariate. The model had adequate fit. Social status insecurity was associated positively with callousness, unemotional, and popularity-motivated aggression and related negatively to popularity-motivated prosocial behaviors. High social status insecurity was related to greater popularity-motivated aggression when adolescents had high callousness traits. The findings have implications for understanding the individual characteristics associated with social status insecurity. Lausanne, Schweiz Frontiers 2020 8 Frontiers in Psychology 1 8 10.3389/fpsyg.2021.606865 Extern OPUS4-56688 Wissenschaftlicher Artikel Kayhan, Ezgi; Matthes, Daniel; Marriott Haresign, Ira; Bánki, Anna; Michel, Christine; Langeloh, Miriam; Wass, Sam; Hoehl, Stefanie DEEP: A dual EEG pipeline for developmental hyperscanning studies Cutting-edge hyperscanning methods led to a paradigm shift in social neuroscience. It allowed researchers to measure dynamic mutual alignment of neural processes between two or more individuals in naturalistic contexts. The ever-growing interest in hyperscanning research calls for the development of transparent and validated data analysis methods to further advance the field. We have developed and tested a dual electroencephalography (EEG) analysis pipeline, namely DEEP. Following the preprocessing of the data, DEEP allows users to calculate Phase Locking Values (PLVs) and cross-frequency PLVs as indices of inter-brain phase alignment of dyads as well as time-frequency responses and EEG power for each participant. The pipeline also includes scripts to control for spurious correlations. Our goal is to contribute to open and reproducible science practices by making DEEP publicly available together with an example mother-infant EEG hyperscanning dataset. Amsterdam, Niederlande Elsevier 2022 11 Developmental Cognitive Neuroscience 54 1 11 10.1016/j.dcn.2022.101104 Extern OPUS4-56171 Wissenschaftlicher Artikel Tiedemann, Kim; Iobbi-Nivol, Chantal; Leimkühler, Silke The Role of the Nucleotides in the Insertion of the bis-Molybdopterin Guanine Dinucleotide Cofactor into apo-Molybdoenzymes The role of the GMP nucleotides of the bis-molybdopterin guanine dinucleotide (bis-MGD) cofactor of the DMSO reductase family has long been a subject of discussion. The recent characterization of the bis-molybdopterin (bis-Mo-MPT) cofactor present in the E. coli YdhV protein, which differs from bis-MGD solely by the absence of the nucleotides, now enables studying the role of the nucleotides of bis-MGD and bis-MPT cofactors in Moco insertion and the activity of molybdoenzymes in direct comparison. Using the well-known E. coli TMAO reductase TorA as a model enzyme for cofactor insertion, we were able to show that the GMP nucleotides of bis-MGD are crucial for the insertion of the bis-MGD cofactor into apo-TorA. 9 Basel, Schweiz MDPI 2022 15 Molecules 27 1 15 10.3390/molecules27092993 Institut für Biochemie und Biologie OPUS4-54897 Wissenschaftlicher Artikel Schaefer, Laura V.; Dech, Silas; Aehle, Markus; Bittmann, Frank Disgusting odours affect the characteristics of the adaptive force in contrast to neutral and pleasant odours The olfactomotor system is especially investigated by examining the sniffing in reaction to olfactory stimuli. The motor output of respiratory-independent muscles was seldomly considered regarding possible influences of smells. The Adaptive Force (AF) characterizes the capability of the neuromuscular system to adapt to external forces in a holding manner and was suggested to be more vulnerable to possible interfering stimuli due to the underlying complex control processes. The aim of this pilot study was to measure the effects of olfactory inputs on the AF of the hip and elbow flexors, respectively. The AF of 10 subjects was examined manually by experienced testers while smelling at sniffing sticks with neutral, pleasant or disgusting odours. The reaction force and the limb position were recorded by a handheld device. The results show, inter alia, a significantly lower maximal isometric AF and a significantly higher AF at the onset of oscillations by perceiving disgusting odours compared to pleasant or neutral odours (p < 0.001). The adaptive holding capacity seems to reflect the functionality of the neuromuscular control, which can be impaired by disgusting olfactory inputs. An undisturbed functioning neuromuscular system appears to be characterized by a proper length tension control and by an earlier onset of mutual oscillations during an external force increase. This highlights the strong connection of olfaction and motor control also regarding respiratory-independent muscles. London Springer Nature 2021 16 Scientific Reports 11 1 16 10.1038/s41598-021-95759-0 Extern OPUS4-329 Teil eines Buches Lück, Erika; Balderjahn, Ingo; Kamm, Birgit; Greil, Holle; Wallschläger, Hans-Dieter; Jessel, Beate; Böckmann, Christine; Oberhänsli, Roland; Soyez, Konrad; Schmeer, Ernst; Blumenstein, Oswald; Berndt, Klaus-Peter; Edeling, Thomas; Friedrich, Sabine; Kaden, Klaus; Scheller, Frieder W.; Petersen, Hans-Georg; Asche, Hartmut; Bronstert, Axel; Giest, Hartmut; Gaedke, Ursula; Löhmannsröben, Hans-Gerd; Jeltsch, Florian; Jänkel, Ralph; Gzik, Axel; Bork, Hans-Rudolf; Bork, Hans-Rudolf Umweltforschung für das Land Brandenburg : Arbeitsgruppen und Professuren 2000 urn:nbn:de:kobv:517-opus-3797 Extern OPUS4-1651 Konferenzveröffentlichung Reimer, O.; Aharonian, Felix A.; Hinton, J.; Hofmann, W.; Hoppe, S.; Raue, M.; Reimer, A. VHE gamma-rays from Westerlund 2 and implications for the inferred energetics The H.E.S.S. collaboration recently reported the discovery of VHE γ-ray emission coincident with the young stellar cluster Westerlund 2. This system is known to host a population of hot, massive stars, and, most particularly, the WR binary WR 20a. Particle acceleration to TeV energies in Westerlund 2 can be accomplished in several alternative scenarios, therefore we only discuss energetic constraints based on the total available kinetic energy in the system, the actual mass loss rates of respective cluster members, and implied gamma-ray production from processes such as inverse Compton scattering or neutral pion decay. From the inferred gammaray luminosity of the order of 1035erg/s, implications for the efficiency of converting available kinetic energy into non-thermal radiation associated with stellar winds in the Westerlund 2 cluster are discussed under consideration of either the presence or absence of wind clumping. 2007 urn:nbn:de:kobv:517-opus-18172 Extern OPUS4-56993 Wissenschaftlicher Artikel Weithoff, Guntram; Bell, Elanor Margaret Complex Trophic Interactions in an Acidophilic Microbial Community Extreme habitats often harbor specific communities that differ substantially from non-extreme habitats. In many cases, these communities are characterized by archaea, bacteria and protists, whereas the number of species of metazoa and higher plants is relatively low. In extremely acidic habitats, mostly prokaryotes and protists thrive, and only very few metazoa thrive, for example, rotifers. Since many studies have investigated the physiology and ecology of individual species, there is still a gap in research on direct, trophic interactions among extremophiles. To fill this gap, we experimentally studied the trophic interactions between a predatory protist (Actinophrys sol, Heliozoa) and its prey, the rotifers Elosa woralli and Cephalodella sp., the ciliate Urosomoida sp. and the mixotrophic protist Chlamydomonas acidophila (a green phytoflagellate, Chlorophyta). We found substantial predation pressure on all animal prey. High densities of Chlamydomonas acidophila reduced the predation impact on the rotifers by interfering with the feeding behaviour of A. sol. These trophic relations represent a natural case of intraguild predation, with Chlamydomonas acidophila being the common prey and the rotifers/ciliate and A. sol being the intraguild prey and predator, respectively. We further studied this intraguild predation along a resource gradient using Cephalodella sp. as the intraguild prey. The interactions among the three species led to an increase in relative rotifer abundance with increasing resource (Chlamydomonas) densities. By applying a series of laboratory experiments, we revealed the complexity of trophic interactions within a natural extremophilic community. 7 Basel, Schweiz MDPI 2022 10 Microorganisms 10 1 10 10.3390/microorganisms10071340 Institut für Biochemie und Biologie OPUS4-43171 Buch (Monographie) Weiß, Norman; Verlaan, Stephanie; Vasquez Carruthers, Juan Francisco; Mair, Theresa; Conner, Sean; Maaser, Lucas; Röthlisberger, Livia Weiß, Norman Transitional Justice This publication deals with the topic of transitional justice. In six case studies, the authors link theoretical and practical implications in order to develop some innovative approaches. Their proposals might help to deal more effectively with the transition of societies, legal orders and political systems. Young academics from various backgrounds provide fresh insights and demonstrate the relevance of the topic. The chapters analyse transitions and conflicts in Sierra Leone, Argentina, Nicaragua, Nepal, and South Sudan as well as Germany's colonial genocide in Namibia. Thus, the book provides the reader with new insights and contributes to the ongoing debate about transitional justice. Potsdam Universitätsverlag Potsdam 2022 194 Potsdamer Studien zu Staat, Recht und Politik 978-3-86956-473-9 7 urn:nbn:de:kobv:517-opus4-431711 10.25932/publishup-43171 Öffentliches Recht OPUS4-57733 Wissenschaftlicher Artikel Prasse, Paul; Iversen, Pascal; Lienhard, Matthias; Thedinga, Kristina; Herwig, Ralf; Scheffer, Tobias Pre-Training on In Vitro and Fine-Tuning on Patient-Derived Data Improves Deep Neural Networks for Anti-Cancer Drug-Sensitivity Prediction Large-scale databases that report the inhibitory capacities of many combinations of candidate drug compounds and cultivated cancer cell lines have driven the development of preclinical drug-sensitivity models based on machine learning. However, cultivated cell lines have devolved from human cancer cells over years or even decades under selective pressure in culture conditions. Moreover, models that have been trained on in vitro data cannot account for interactions with other types of cells. Drug-response data that are based on patient-derived cell cultures, xenografts, and organoids, on the other hand, are not available in the quantities that are needed to train high-capacity machine-learning models. We found that pre-training deep neural network models of drug sensitivity on in vitro drug-sensitivity databases before fine-tuning the model parameters on patient-derived data improves the models' accuracy and improves the biological plausibility of the features, compared to training only on patient-derived data. From our experiments, we can conclude that pre-trained models outperform models that have been trained on the target domains in the vast majority of cases. 16 Basel, Schweiz MDPI 2022 14 MDPI 14 1 14 10.3390/cancers14163950 Institut für Informatik und Computational Science OPUS4-53685 Wissenschaftlicher Artikel Omane, Paul Okyere; Höhle, Barbara Acquiring syntactic variability This paper investigates the predictions of the Derivational Complexity Hypothesis by studying the acquisition of wh-questions in 4- and 5-year-old Akan-speaking children in an experimental approach using an elicited production and an elicited imitation task. Akan has two types of wh-question structures (wh-in-situ and wh-ex-situ questions), which allows an investigation of children's acquisition of these two question structures and their preferences for one or the other. Our results show that adults prefer to use wh-ex-situ questions over wh-in-situ questions. The results from the children show that both age groups have the two question structures in their linguistic repertoire. However, they differ in their preferences in usage in the elicited production task: while the 5-year-olds preferred the wh-in-situ structure over the wh-ex-situ structure, the 4-year-olds showed a selective preference for the wh-in-situ structure in who-questions. These findings suggest a developmental change in wh-question preferences in Akan-learning children between 4 and 5 years of age with a so far unobserved u-shaped developmental pattern. In the elicited imitation task, all groups showed a strong tendency to maintain the structure of in-situ and ex-situ questions in repeating grammatical questions. When repairing ungrammatical ex-situ questions, structural changes to grammatical in-situ questions were hardly observed but the insertion of missing morphemes while keeping the ex-situ structure. Together, our findings provide only partial support for the Derivational Complexity Hypothesis. Lausanne, Schweiz Frontiers Media 2020 15 Frontiers in communication 2021 10.3389/fcomm.2021.604951 Extern OPUS4-1709 Dissertation Müller, Melanie J. I. Bidirectional transport by molecular motors In biological cells, the long-range intracellular traffic is powered by molecular motors which transport various cargos along microtubule filaments. The microtubules possess an intrinsic direction, having a 'plus' and a 'minus' end. Some molecular motors such as cytoplasmic dynein walk to the minus end, while others such as conventional kinesin walk to the plus end. Cells typically have an isopolar microtubule network. This is most pronounced in neuronal axons or fungal hyphae. In these long and thin tubular protrusions, the microtubules are arranged parallel to the tube axis with the minus ends pointing to the cell body and the plus ends pointing to the tip. In such a tubular compartment, transport by only one motor type leads to 'motor traffic jams'. Kinesin-driven cargos accumulate at the tip, while dynein-driven cargos accumulate near the cell body. We identify the relevant length scales and characterize the jamming behaviour in these tube geometries by using both Monte Carlo simulations and analytical calculations. A possible solution to this jamming problem is to transport cargos with a team of plus and a team of minus motors simultaneously, so that they can travel bidirectionally, as observed in cells. The presumably simplest mechanism for such bidirectional transport is provided by a 'tug-of-war' between the two motor teams which is governed by mechanical motor interactions only. We develop a stochastic tug-of-war model and study it with numerical and analytical calculations. We find a surprisingly complex cooperative motility behaviour. We compare our results to the available experimental data, which we reproduce qualitatively and quantitatively. 2008 urn:nbn:de:kobv:517-opus-18715 Institut für Physik und Astronomie OPUS4-54871 Wissenschaftlicher Artikel Rose, Robert; Groeger, Lars; Hölzle, Katharina The Emergence of Shared Leadership in Innovation Labs Implementing innovation laboratories to leverage intrapreneurship are an increasingly popular organizational practice. A typical feature in these creative environments are semi-autonomous teams in which multiple members collectively exert leadership influence, thereby challenging traditional command-and-control conceptions of leadership. An extensive body of research on the team-centric concept of shared leadership has recognized the potential for pluralized leadership structures in enhancing team effectiveness; however, little empirical work has been conducted in organizational contexts in which creativity is key. This study set out to explore antecedents of shared leadership and its influence on team creativity in an innovation lab. Building on extant shared leadership and innovation research, we propose antecedents customary to creative teamwork, that is, experimental culture, task reflexivity, and voice. Multisource data were collected from 104 team members and 49 evaluations of 29 coaches nested in 21 teams working in a prototypical innovation lab. We identify factors specific to creative teamwork that facilitate the emergence of shared leadership by providing room for experimentation, encouraging team members to speak up in the creative process, and cultivating a reflective application of entrepreneurial thinking. We provide specific exemplary activities for innovation lab teams to increase levels of shared leadership. Lausanne, Schweiz Frontiers in psychology 2021 13 Frontiers in Psychology 12 1 13 10.3389/fpsyg.2021.685167 Extern OPUS4-56555 Wissenschaftlicher Artikel Dordevic, Milos; Hölzer, Sonja; Russo, Augusta; García Alanis, José Carlos; Müller, Notger Germar The Role of the Precuneus in Human Spatial Updating in a Real Environment Setting—A cTBS Study As we move through an environment, we update positions of our body relative to other objects, even when some objects temporarily or permanently leave our field of view—this ability is termed egocentric spatial updating and plays an important role in everyday life. Still, our knowledge about its representation in the brain is still scarce, with previous studies using virtual movements in virtual environments or patients with brain lesions suggesting that the precuneus might play an important role. However, whether this assumption is also true when healthy humans move in real environments where full body-based cues are available in addition to the visual cues typically used in many VR studies is unclear. Therefore, in this study we investigated the role of the precuneus in egocentric spatial updating in a real environment setting in 20 healthy young participants who underwent two conditions in a cross-over design: (a) stimulation, achieved through applying continuous theta-burst stimulation (cTBS) to inhibit the precuneus and (b) sham condition (activated coil turned upside down). In both conditions, participants had to walk back with blindfolded eyes to objects they had previously memorized while walking with open eyes. Simplified trials (without spatial updating) were used as control condition, to make sure the participants were not affected by factors such as walking blindfolded, vestibular or working memory deficits. A significant interaction was found, with participants performing better in the sham condition compared to real stimulation, showing smaller errors both in distance and angle. The results of our study reveal evidence of an important role of the precuneus in a real-environment egocentric spatial updating; studies on larger samples are necessary to confirm and further investigate this finding. 8 Basel, Schweiz MDPI 2022 13 Life 12 1 13 10.3390/life12081239 Extern OPUS4-56553 Wissenschaftlicher Artikel Westphal, Andrea; Kalinowski, Eva; Hoferichter, Clara Josepha; Vock, Miriam K−12 teachers' stress and burnout during the COVID-19 pandemic: A systematic review We present the first systematic literature review on stress and burnout in K−12 teachers during the COVID-19 pandemic. Based on a systematic literature search, we identified 17 studies that included 9,874 K−12 teachers from around the world. These studies showed some indication that burnout did increase during the COVID-19 pandemic. There were, however, almost no differences in the levels of stress and burnout experienced by K−12 teachers compared to individuals employed in other occupational fields. School principals' leadership styles emerged as an organizational characteristic that is highly relevant for K−12 teachers' levels of stress and burnout. Individual teacher characteristics associated with burnout were K−12 teachers' personality, self-efficacy in online teaching, and perceived vulnerability to COVID-19. In order to reduce stress, there was an indication that stress-management training in combination with training in technology use for teaching may be superior to stress-management training alone. Future research needs to adopt more longitudinal designs and examine the interplay between individual and organizational characteristics in the development of teacher stress and burnout during the COVID-19 pandemic and beyond. Lausanne, Schweiz Frontiers 2022 29 Frontiers in Psychology 1 29 10.3389/fpsyg.2022.920326 Extern OPUS4-54887 Wissenschaftlicher Artikel Spikes, Montrai; Rodríguez-Silva, Rodet; Bennett, Kerri-Ann; Bräger, Stefan; Josaphat, James; Torres-Pineda, Patricia; Ernst, Anja; Havenstein, Katja; Schlupp, Ingo; Tiedemann, Ralph A phylogeny of the genus Limia (Teleostei: Poeciliidae) suggests a single-lake radiation nested in a Caribbean-wide allopatric speciation scenario Objective The Caribbean is an important global biodiversity hotspot. Adaptive radiations there lead to many speciation events within a limited period and hence are particularly prominent biodiversity generators. A prime example are freshwater fish of the genus Limia, endemic to the Greater Antilles. Within Hispaniola, nine species have been described from a single isolated site, Lake Miragoâne, pointing towards extraordinary sympatric speciation. This study examines the evolutionary history of the Limia species in Lake Miragoâne, relative to their congeners throughout the Caribbean. Results For 12 Limia species, we obtained almost complete sequences of the mitochondrial cytochrome b gene, a well-established marker for lower-level taxonomic relationships. We included sequences of six further Limia species from GenBank (total N  = 18 species). Our phylogenies are in concordance with other published phylogenies of Limia. There is strong support that the species found in Lake Miragoâne in Haiti are monophyletic, confirming a recent local radiation. Within Lake Miragoâne, speciation is likely extremely recent, leading to incomplete lineage sorting in the mtDNA. Future studies using multiple unlinked genetic markers are needed to disentangle the relationships within the Lake Miragoâne clade. London BMC Research Notes / Biomed Central 2021 8 BMC Research Notes 14 1 8 10.1186/s13104-021-05843-x Institut für Biochemie und Biologie OPUS4-55006 Wissenschaftlicher Artikel Krüger, Johanna; Foerster, Verena Elisabeth; Trauth, Martin H.; Hofreiter, Michael; Tiedemann, Ralph Exploring the Past Biosphere of Chew Bahir/Southern Ethiopia: Cross-Species Hybridization Capture of Ancient Sedimentary DNA from a Deep Drill Core Eastern Africa has been a prime target for scientific drilling because it is rich in key paleoanthropological sites as well as in paleolakes, containing valuable paleoclimatic information on evolutionary time scales. The Hominin Sites and Paleolakes Drilling Project (HSPDP) explores these paleolakes with the aim of reconstructing environmental conditions around critical episodes of hominin evolution. Identification of biological taxa based on their sedimentary ancient DNA (sedaDNA) traces can contribute to understand past ecological and climatological conditions of the living environment of our ancestors. However, sedaDNA recovery from tropical environments is challenging because high temperatures, UV irradiation, and desiccation result in highly degraded DNA. Consequently, most of the DNA fragments in tropical sediments are too short for PCR amplification. We analyzed sedaDNA in the upper 70 m of the composite sediment core of the HSPDP drill site at Chew Bahir for eukaryotic remnants. We first tested shotgun high throughput sequencing which leads to metagenomes dominated by bacterial DNA of the deep biosphere, while only a small fraction was derived from eukaryotic, and thus probably ancient, DNA. Subsequently, we performed cross-species hybridization capture of sedaDNA to enrich ancient DNA (aDNA) from eukaryotic remnants for paleoenvironmental analysis, using established barcoding genes (cox1 and rbcL for animals and plants, respectively) from 199 species that may have had relatives in the past biosphere at Chew Bahir. Metagenomes yielded after hybridization capture are richer in reads with similarity to cox1 and rbcL in comparison to metagenomes without prior hybridization capture. Taxonomic assignments of the reads from these hybridization capture metagenomes also yielded larger fractions of the eukaryotic domain. For reads assigned to cox1, inferred wet periods were associated with high inferred relative abundances of putative limnic organisms (gastropods, green algae), while inferred dry periods showed increased relative abundances for insects. These findings indicate that cross-species hybridization capture can be an effective approach to enhance the information content of sedaDNA in order to explore biosphere changes associated with past environmental conditions, enabling such analyses even under tropical conditions. Lausanne, Schweiz Frontiers in Earth Science 2021 20 Frontiers in Earth Science 1 20 10.3389/feart.2021.683010 Institut für Biochemie und Biologie OPUS4-3967 Konferenzveröffentlichung Banda, Gourinath; Gallagher, John P. Constraint-based abstraction of a model checker for infinite state systems Abstract interpretation-based model checking provides an approach to verifying properties of infinite-state systems. In practice, most previous work on abstract model checking is either restricted to verifying universal properties, or develops special techniques for temporal logics such as modal transition systems or other dual transition systems. By contrast we apply completely standard techniques for constructing abstract interpretations to the abstraction of a CTL semantic function, without restricting the kind of properties that can be verified. Furthermore we show that this leads directly to implementation of abstract model checking algorithms for abstract domains based on constraints, making use of an SMT solver. 2010 urn:nbn:de:kobv:517-opus-41516 Extern OPUS4-5224 Wissenschaftlicher Artikel Kohnen, Saskia Vernachlässigte Dyslexien 2011 6 Spektrum Patholinguistik 4 75 81 urn:nbn:de:kobv:517-opus-54226 Extern OPUS4-5226 Wissenschaftlicher Artikel Bethmann, Anja; Scheich, Henning; Brechmann, André Produktion von Eigennamen 2011 21 Spektrum Patholinguistik 4 95 116 urn:nbn:de:kobv:517-opus-54246 Extern OPUS4-5231 Wissenschaftlicher Artikel Meinusch, Miriam; Neumann, Sandra Systematisches Review zur Ermittlung wissenschaftlicher Evidenzen der Dysphagie bei LKGS-Fehlbildungen 2011 21 Spektrum Patholinguistik 4 149 170 urn:nbn:de:kobv:517-opus-54291 Extern OPUS4-5234 Wissenschaftlicher Artikel Puritz, Caroline; Seidl, Rainer Ottis; Frank, Ulrike Die Auswirkungen des Lee Silverman Voice Treatments (LSVT) auf die kortikalen Repräsentationen der Schluckmuskulatur bei Patienten mit Morbus Parkinson 2011 2 Spektrum Patholinguistik 4 189 191 urn:nbn:de:kobv:517-opus-54322 Extern OPUS4-5235 Wissenschaftlicher Artikel Etzien, Maria; Machleb, Franziska; Lorenz, Antje Semantische versus wortform-spezifische Merkmalsanalyse in der Behandlung von Wortabrufstörungen bei Aphasie 2011 4 Spektrum Patholinguistik 4 193 197 urn:nbn:de:kobv:517-opus-54337 Extern OPUS4-5238 Wissenschaftlicher Artikel Tagoe, Tanja Sprache und Emotion bei Kindern und Jugendlichen mit Williams-Beuren-Syndrom 2011 19 Spektrum Patholinguistik 4 217 236 urn:nbn:de:kobv:517-opus-54367 Extern OPUS4-5239 Wissenschaftlicher Artikel Watermeyer, Melanie; Höhle, Barbara; Kauschke, Christina Ausagieren von Sätzen versus Satz-Bild-Zuordnung 2011 9 Spektrum Patholinguistik 4 237 246 urn:nbn:de:kobv:517-opus-54374 Extern OPUS4-5557 Buch (Monographie) Peters, Friedrich Ernst Die dröge Trina : Geschichte einer "Poahr Dangoaß" "Die dröge Trina" erzählt eine von F. E. Peters in hochdeutscher Sprache veröffentlichte Geschichte aus der "Baasdörper Krönk", der bedeutenden niederdeutschen Dorfchronik desselben Autors. Der Roman, 1946 erschienen, beschreibt, wie die Bauerntochter Trina Dührsen, raffgierige Anbeterin des "Taler-Gottes", durch ihre Habgier das Leben ihres Bruders und ihres Ehemannes zerstört und versucht, ihren Neffen Wilhelm um sein Erbe zu bringen. Der Roman findet dennoch ein versöhnliches Ende, als Trina inmitten der Inflation der Jahre 1922/1923 die Entwertung ihres Geldes und damit die Vergänglichkeit irdischer Güter erlebt, gleichzeitig aber menschliche Werte schätzen lernt . Der eigenwillige Untertitel "Geschichte einer Poahr Dangoaß" bezieht sich auf den Spitz- und Schimpfnamen Trinas, eine Verballhornung des französischen Begriffs "poire d'angoisse" (für das Folterinstrument der Mundbirne), der ihr in Anspielung an ihren schlechten Charakter und die schönen, aber wenig schmackhaften Birnen ihres Hofes, die "Gurgelschnürer", verliehen wurde. "Die dröge Trina" schildert mit freundlich-ironischer Empathie mentalitätsgeschichtlich aufschlussreiche Verhaltensweisen, Sitten und Bräuche der norddeutschen Provinz im Zeitalter der Industrialisierung. Berühmtheit hat die Beschreibung eines Schweinewiegens erlangt. Die dröge Trina ist 1945 als Fortsetzungsroman unter dem Titel "Trina Dührsen" in den Flensburger Nachrichten erschienen. 2012 urn:nbn:de:kobv:517-opus-57245 Extern OPUS4-5558 Buch (Monographie) Peters, Friedrich Ernst Die schmale Brücke "Die schmale Brücke" ist eine Dreiecksgeschichte, die das Ringen zweier ungleicher Männer um eine junge Frau schildert. Der fleißige und solide Mittelschulrektor Peter Hansen, der unter der Kinderlosigkeit und Kälte seiner Frau leidet, trifft nach dem Ersten Weltkrieg seinen "Jugendfeind", den redegewandten, bei den Damen hoch beliebten Werner Hagemann wieder, welcher im Zug die Lehrerin Ursula Wüstenhagen kennengelernt hat, eine Kollegin Peter Hansens, in die dieser sich verliebt hat. In schwüler Gesprächsatmosphäre entwickelt sich eine Rivalität, die zunehmend bedrohliche Züge annimmt. Mordgedanken keimen auf. Schließlich fordert Peter Hansen, auf ein Gottesurteil hoffend, Werner Hagemann zu einem lebensgefährlichen Kräftemessen auf einer Brücke auf. Die Erzählung ist das einzige Werk von F. E. Peters, das im Lehrermilieu spielt. Verwoben mit der spannungsreichen Dreiecksbeziehung sind Themen der Frauenemanzipation und modernen Pädagogik sowie zahlreiche literarische Reminiszenzen an den "Zauberberg" oder auch an Goethe und Hölderlin, insbesondere in der zentralen Frage der "Schicksalswürdigkeit". Der ursprünglich von Peters gewählte Titel ("Der Schicksalswürdige") wurde auf Wunsch des Verlages in "Die schmale Brücke" geändert. 2012 urn:nbn:de:kobv:517-opus-57258 Extern OPUS4-5559 Buch (Monographie) Peters, Friedrich Ernst Der heilsame Umweg „Der heilsame Umweg", 1939 mit dem Schleswig-Holsteinischen Literaturpreis ausgezeichnet, gehört zu der Gattung der deutsch-französischen Verständigungsromane, die in den dreißiger Jahren des 20. Jahrhunderts sehr erfolgreich waren. Der Roman beschreibt das Leben französischer Kriegsgefangener in einem holsteinischen Dorf während des Ersten Weltkriegs und ist das fiktionale Pendant zu den Kriegserinnerungen von F.E. Peters, der selbst von 1914 bis 1920 Gefangener in Frankreich war und dort als Dolmetscher eingesetzt wurde. Im Mittelpunkt der Handlung steht die junge Elisabeth Tormählen, die sich von der weltfremden Schülerin eines Mädchenpensionats zu einer tüchtigen Bäuerin und reifen Ehefrau entwickelt. Während ihr Mann an der Front ist, entspinnt sich eine kurze harmlose Romanze zwischen ihr und dem französischen Dolmetscher Gaston Marzais, die schnell beendet ist, als Elisabeth endgültig zu ihrem Mann findet und dieser sich nach Kriegsende von ihrer Treue überzeugen kann. Das vielschichtige Werk beschreibt unter Verzicht auf die gängigen nationalen Stereotypen oder mittels deren Demontage Deutsche und Franzosen als in ihrer Unterschiedlichkeit sehr ähnlich. Durch zahlreiche Symmetrie-Effekte und konsequent durchgeführte Perspektivwechsel, die sowohl die deutsche als auch die französische Sicht zu berücksichtigen suchen, wird das Gleiche im Fremden hervorgehoben. Gleicher Patriotismus und gleiche Opferbereitschaft auf beiden Seiten, aber auch gleiche Friedenssehnsüchte und gleiches Leiden unter den Grausamkeiten des Krieges sowie gleiche fachliche Kompetenz - denn auch die gefangenen Franzosen sind erfahrene Bauern und bewähren sich im täglichen Zusammenleben. Fanatiker wie die verbitterte Soldatenwitwe Greta Harders oder der hasserfüllte Brandstifter Maurice Thaudière werden als lebensuntüchtig dargestellt und begehen Selbstmord. Den ideologischen Grundtenor des Werkes betonen zum Schluss noch einmal die Intellektuellen: Gaston Marzais und sein deutscher Freund, der Jurist Georg Schmitt, beide erfüllt von dem Wissen um und dem Respekt vor der Kultur des anderen. Gemeinsam wollen sie nach der deutschen Niederlage weitere deutsch-französische Kriege verhindern und beschwören die pazifistische Vision eines Europas der Toleranz und der Aufklärung. „Der heilsame Umweg" ist darüber hinaus eine Hymne an bäuerliche Kultur und familiäre Werte. So bewundert Marzais Interieur und Kleidung der alten Bäuerin Abel Schierholdt. Auch ein traditionelles norddeutsches Begräbnisritual wird detailliert beschrieben. Frankreichs Landleben ist präsent durch nostalgische Evozierungen der Gefangenen und deutliche Anklänge an den am Anfang des letzten Jahrhunderts sehr beliebten Roman von Henry Bordeaux „Les Roquevillard" (1906), den F.E.Peters während seiner Kriegsgefangenschaft übersetzt hat. Schließlich reflektiert der Roman Macht und Ohnmacht von Sprache sowie die autobiographische Dimension literarischer Produktions- und Rezeptionsprozesse. Seine Heldin - und mit ihr der Leser - erkennt, "dass Dichtung nur echt ist als erlittenes Leben und dass sie nur aus der Erfahrung des Leides verstanden wird." 2012 urn:nbn:de:kobv:517-opus-57261 Extern OPUS4-5613 Wissenschaftlicher Artikel Peters, Friedrich Ernst "Ulenspegel un Jan Dood" Charakterisierung des literarischen Schaffens von Moritz Jahn. Seine Bedeutung für das Plattdeutsche. F. E. Peters beschreibt, Moritz Jahn humorvoll folgend, "polare Spannungen" in Niederdeutschland: "Es gibt Niederdeutsche, die ihrem Lande nie so ganz trauen, die daheim in einer immerwährenden leisen Unruhe hinleben und die aufatmen, wenn sie in gebirgiger Gegend das nackte Gestein unter ihren Füßen fühlen. Dann wird klar, was sie sich bis dahin mühsam verhehlt haben: dass sie nämlich in einem geheimen Winkel ihrer Seele glauben, ihr Land sei vom Meere unterspült, könne an besonders dünnen Stellen durchbrechen, vom festen Kern der Erde losgerissen werden und in die Nebel der Unendlichkeit hinaustreiben. Auf felsigem Grund werden sie dann die neue und wohltuende Sicherheit mit großer Freude so lange genießen, bis das unwiderstehliche Heimweh sie zurücktreibt in die Unsicherheit." Potsdam Universitätsverlag Potsdam 2012 Digitale Edition : Friedrich Peters urn:nbn:de:kobv:517-opus-58086 Extern OPUS4-5605 Dissertation Krause, Jette An expert-based Bayesian investigation of greenhouse gas emission reduction options for German passenger vehicles until 2030 The present thesis introduces an iterative expert-based Bayesian approach for assessing greenhouse gas (GHG) emissions from the 2030 German new vehicle fleet and quantifying the impacts of their main drivers. A first set of expert interviews has been carried out in order to identify technologies which may help to lower car GHG emissions and to quantify their emission reduction potentials. Moreover, experts were asked for their probability assessments that the different technologies will be widely adopted, as well as for important prerequisites that could foster or hamper their adoption. Drawing on the results of these expert interviews, a Bayesian Belief Network has been built which explicitly models three vehicle types: Internal Combustion Engine Vehicles (which include mild and full Hybrid Electric Vehicles), Plug-In Hybrid Electric Vehicles, and Battery Electric Vehicles. The conditional dependencies of twelve central variables within the BBN - battery energy, fuel and electricity consumption, relative costs, and sales shares of the vehicle types - have been quantified by experts from German car manufacturers in a second series of interviews. For each of the seven second-round interviews, an expert's individually specified BBN results. The BBN have been run for different hypothetical 2030 scenarios which differ, e.g., in regard to battery development, regulation, and fuel and electricity GHG intensities. The present thesis delivers results both in regard to the subject of the investigation and in regard to its method. On the subject level, it has been found that the different experts expect 2030 German new car fleet emission to be at 50 to 65% of 2008 new fleet emissions under the baseline scenario. They can be further reduced to 40 to 50% of the emissions of the 2008 fleet though a combination of a higher share of renewables in the electricity mix, a larger share of biofuels in the fuel mix, and a stricter regulation of car CO$_2$ emissions in the European Union. Technically, 2030 German new car fleet GHG emissions can be reduced to a minimum of 18 to 44% of 2008 emissions, a development which can not be triggered by any combination of measures modeled in the BBN alone but needs further commitment. Out of a wealth of existing BBN, few have been specified by individual experts through elicitation, and to my knowledge, none of them has been employed for analyzing perspectives for the future. On the level of methods, this work shows that expert-based BBN are a valuable tool for making experts' expectations for the future explicit and amenable to the analysis of different hypothetical scenarios. BBN can also be employed for quantifying the impacts of main drivers. They have been demonstrated to be a valuable tool for iterative stakeholder-based science approaches. 2011 urn:nbn:de:kobv:517-opus-57671 Wirtschaftswissenschaften OPUS4-5607 Wissenschaftlicher Artikel Peters, Friedrich Ernst Meissenheim Beschreibung einer Reise durch das Elsass verflochten mit Zitaten aus F. Hölderlins Gedicht „Der Rhein" (1801) und Entdeckung des Friederike-Brion-Grabes in Meißenheim. Peters entzaubert den Friederike-Mythos mit einer empathisch-kritischen Frage, die literarische Überhöhung und Lebenswirklichkeit kontrastiert: „Dem Mädchen aus Sesenheim geschah Leid von dem Halbgott. Leid war der Preis für das Licht der Unsterblichkeit,… War der Preis zu hoch?" 2012 Digitale Edition : Friedrich Peters urn:nbn:de:kobv:517-opus-57979 Extern OPUS4-5608 Wissenschaftlicher Artikel Peters, Friedrich Ernst Timm Kröger Der norddeutsche Schriftsteller Timm Kröger ist in Haale (Kreis Rendsburg-Eckernförde) geboren, einem Dorf, das in nächster Nähe von Luhnstedt, dem Heimatdorf von F.E. Peters, liegt. Peters und Kröger sind sich nie persönlich begegnet, hatten aber gemeinsame Bekannte. „Timm Kröger - der Dichter unserer Heimat" stammt aus dem Nachlass von F.E. Peters, der ein Bewunderer der Kunst Timm Krögers war und den „besonderen lyrischen Schimmer" seiner Erzählkunst geliebt hat. Die Rede ist anlässlich des 110. Geburtstages von Timm Kröger entstanden. Peters nimmt das Jubiläum zum Anlass, anhand von Zitaten aus Krögers Vorwort zu einer Gesamtausgabe seiner Werke die Gattung Heimatdichtung kritisch zu beleuchten und zu unterscheiden zwischen einer ideologisch gefärbten schablonenhaften Heimatdichtung und einer Dichtung, welche die Landschaften der Heimat in den Mittelpunkt stellt, das Provinzielle jedoch überwindet: „Es sei zugegeben, dass von sogenannten Heimatdichtern viel plattes Zeug geliefert wird. Darum spreche ich heute von dem Dichter unserer Heimat, nicht von dem Heimatdichter Timm Kröger." Potsdam Universitätsverlag Potsdam 2012 Digitale Edition : Friedrich Peters urn:nbn:de:kobv:517-opus-57984 Extern