@article{BalintMartonSchatzetal.2018, author = {Balint, Miklos and Marton, Orsolya and Schatz, Marlene and D{\"u}ring, Rolf-Alexander and Grossart, Hans-Peter}, title = {Proper experimental design requires randomization/balancing of molecular ecology experiments}, series = {Ecology and evolution}, volume = {8}, journal = {Ecology and evolution}, number = {3}, publisher = {Wiley}, address = {Hoboken}, issn = {2045-7758}, doi = {10.1002/ece3.3687}, pages = {1786 -- 1793}, year = {2018}, abstract = {Properly designed (randomized and/or balanced) experiments are standard in ecological research. Molecular methods are increasingly used in ecology, but studies generally do not report the detailed design of sample processing in the laboratory. This may strongly influence the interpretability of results if the laboratory procedures do not account for the confounding effects of unexpected laboratory events. We demonstrate this with a simple experiment where unexpected differences in laboratory processing of samples would have biased results if randomization in DNA extraction and PCR steps do not provide safeguards. We emphasize the need for proper experimental design and reporting of the laboratory phase of molecular ecology research to ensure the reliability and interpretability of results.}, language = {en} } @article{BizicIonescuIonescuGrossart2018, author = {Bizic-Ionescu, Mina and Ionescu, Danny and Grossart, Hans-Peter}, title = {Organic Particles: Heterogeneous Hubs for Microbial Interactions in Aquatic Ecosystems}, series = {Frontiers in microbiology}, volume = {9}, journal = {Frontiers in microbiology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-302X}, doi = {10.3389/fmicb.2018.02569}, pages = {15}, year = {2018}, abstract = {The dynamics and activities of microbes colonizing organic particles (hereafter particles) greatly determine the efficiency of the aquatic carbon pump. Current understanding is that particle composition, structure and surface properties, determined mostly by the forming organisms and organic matter, dictate initial microbial colonization and the subsequent rapid succession events taking place as organic matter lability and nutrient content change with microbial degradation. We applied a transcriptomic approach to assess the role of stochastic events on initial microbial colonization of particles. Furthermore, we asked whether gene expression corroborates rapid changes in carbon-quality. Commonly used size fractionated filtration averages thousands of particles of different sizes, sources, and ages. To overcome this drawback, we used replicate samples consisting each of 3-4 particles of identical source and age and further evaluated the consequences of averaging 10-1000s of particles. Using flow-through rolling tanks we conducted long-term experiments at near in situ conditions minimizing the biasing effects of closed incubation approaches often referred to as "the bottle-effect." In our open flow-through rolling tank system, however, active microbial communities were highly heterogeneous despite an identical particle source, suggesting random initial colonization. Contrasting previous reports using closed incubation systems, expression of carbon utilization genes didn't change after 1 week of incubation. Consequently, we suggest that in nature, changes in particle-associated community related to carbon availability are much slower (days to weeks) due to constant supply of labile, easily degradable organic matter. Initial, random particle colonization seems to be subsequently altered by multiple organismic interactions shaping microbial community interactions and functional dynamics. Comparative analysis of thousands particles pooled togethers as well as pooled samples suggests that mechanistic studies of microbial dynamics should be done on single particles. The observed microbial heterogeneity and inter-organismic interactions may have important implications for evolution and biogeochemistry in aquatic systems.}, language = {en} } @article{WurzbacherFuchsAttermeyeretal.2017, author = {Wurzbacher, Christian and Fuchs, Andrea and Attermeyer, Katrin and Frindte, Katharina and Grossart, Hans-Peter and Hupfer, Michael and Casper, Peter and Monaghan, Michael T.}, title = {Shifts among Eukaryota, Bacteria, and Archaea define the vertical organization of a lake sediment}, series = {Microbiome}, volume = {5}, journal = {Microbiome}, publisher = {BioMed Central}, address = {London}, issn = {2049-2618}, doi = {10.1186/s40168-017-0255-9}, pages = {16}, year = {2017}, abstract = {Background: Lake sediments harbor diverse microbial communities that cycle carbon and nutrients while being constantly colonized and potentially buried by organic matter sinking from the water column. The interaction of activity and burial remained largely unexplored in aquatic sediments. We aimed to relate taxonomic composition to sediment biogeochemical parameters, test whether community turnover with depth resulted from taxonomic replacement or from richness effects, and to provide a basic model for the vertical community structure in sediments. Methods: We analyzed four replicate sediment cores taken from 30-m depth in oligo-mesotrophic Lake Stechlin in northern Germany. Each 30-cm core spanned ca. 170 years of sediment accumulation according to Cs-137 dating and was sectioned into layers 1-4 cm thick. We examined a full suite of biogeochemical parameters and used DNA metabarcoding to examine community composition of microbial Archaea, Bacteria, and Eukaryota. Results: Community beta-diversity indicated nearly complete turnover within the uppermost 30 cm. We observed a pronounced shift from Eukaryota- and Bacteria-dominated upper layers (<5 cm) to Bacteria-dominated intermediate layers (5-14 cm) and to deep layers (>14 cm) dominated by enigmatic Archaea that typically occur in deep-sea sediments. Taxonomic replacement was the prevalent mechanism in structuring the community composition and was linked to parameters indicative of microbial activity (e.g., CO2 and CH4 concentration, bacterial protein production). Richness loss played a lesser role but was linked to conservative parameters (e.g., C, N, P) indicative of past conditions. Conclusions: By including all three domains, we were able to directly link the exponential decay of eukaryotes with the active sediment microbial community. The dominance of Archaea in deeper layers confirms earlier findings from marine systems and establishes freshwater sediments as a potential low-energy environment, similar to deep sea sediments. We propose a general model of sediment structure and function based on microbial characteristics and burial processes. An upper "replacement horizon" is dominated by rapid taxonomic turnover with depth, high microbial activity, and biotic interactions. A lower "depauperate horizon" is characterized by low taxonomic richness, more stable "low-energy" conditions, and a dominance of enigmatic Archaea.}, language = {en} } @article{GoeritzBergerGegeetal.2018, author = {G{\"o}ritz, Anna and Berger, Stella A. and Gege, Peter and Grossart, Hans-Peter and Nejstgaard, Jens C. and Riedel, Sebastian and R{\"o}ttgers, R{\"u}diger and Utschig, Christian}, title = {Retrieval of water constituents from hyperspectral in-situ measurements under variable cloud cover}, series = {Remote sensing / Molecular Diversity Preservation International (MDPI)}, volume = {10}, journal = {Remote sensing / Molecular Diversity Preservation International (MDPI)}, number = {2}, publisher = {MDPI}, address = {Basel}, issn = {2072-4292}, doi = {10.3390/rs10020181}, pages = {19}, year = {2018}, abstract = {Remote sensing and field spectroscopy of natural waters is typically performed under clear skies, low wind speeds and low solar zenith angles. Such measurements can also be made, in principle, under clouds and mixed skies using airborne or in-situ measurements; however, variable illumination conditions pose a challenge to data analysis. In the present case study, we evaluated the inversion of hyperspectral in-situ measurements for water constituent retrieval acquired under variable cloud cover. First, we studied the retrieval of Chlorophyll-a (Chl-a) concentration and colored dissolved organic matter (CDOM) absorption from in-water irradiance measurements. Then, we evaluated the errors in the retrievals of the concentration of total suspended matter (TSM), Chl-a and the absorption coefficient of CDOM from above-water reflectance measurements due to highly variable reflections at the water surface. In order to approximate cloud reflections, we extended a recent three-component surface reflectance model for cloudless atmospheres by a constant offset and compared different surface reflectance correction procedures. Our findings suggest that in-water irradiance measurements may be used for the analysis of absorbing compounds even under highly variable weather conditions. The extended surface reflectance model proved to contribute to the analysis of above-water reflectance measurements with respect to Chl-a and TSM. Results indicate the potential of this approach for all-weather monitoring.}, language = {en} } @article{CuadratIonescuDavilaetal.2018, author = {Cuadrat, Rafael R. C. and Ionescu, Danny and Davila, Alberto M. R. and Grossart, Hans-Peter}, title = {Recovering genomics clusters of secondary metabolites from lakes using genome-resolved metagenomics}, series = {Frontiers in microbiology}, volume = {9}, journal = {Frontiers in microbiology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-302X}, doi = {10.3389/fmicb.2018.00251}, pages = {13}, year = {2018}, abstract = {Metagenomic approaches became increasingly popular in the past decades due to decreasing costs of DNA sequencing and bioinformatics development. So far, however, the recovery of long genes coding for secondary metabolites still represents a big challenge. Often, the quality of metagenome assemblies is poor, especially in environments with a high microbial diversity where sequence coverage is low and complexity of natural communities high. Recently, new and improved algorithms for binning environmental reads and contigs have been developed to overcome such limitations. Some of these algorithms use a similarity detection approach to classify the obtained reads into taxonomical units and to assemble draft genomes. This approach, however, is quite limited since it can classify exclusively sequences similar to those available (and well classified) in the databases. In this work, we used draft genomes from Lake Stechlin, north-eastern Germany, recovered by MetaBat, an efficient binning tool that integrates empirical probabilistic distances of genome abundance, and tetranucleotide frequency for accurate metagenome binning. These genomes were screened for secondary metabolism genes, such as polyketide synthases (PKS) and non-ribosomal peptide synthases (NRPS), using the Anti-SMASH and NAPDOS workflows. With this approach we were able to identify 243 secondary metabolite clusters from 121 genomes recovered from our lake samples. A total of 18 NRPS, 19 PKS, and 3 hybrid PKS/NRPS clusters were found. In addition, it was possible to predict the partial structure of several secondary metabolite clusters allowing for taxonomical classifications and phylogenetic inferences. Our approach revealed a high potential to recover and study secondary metabolites genes from any aquatic ecosystem.}, language = {en} } @article{XiaoLiuWangetal.2020, author = {Xiao, Shangbin and Liu, Liu and Wang, Wei and Lorke, Andreas and Woodhouse, Jason Nicholas and Grossart, Hans-Peter}, title = {A Fast-Response Automated Gas Equilibrator (FaRAGE) for continuous in situ measurement of CH4 and CO2 dissolved in water}, series = {Hydrology and earth system sciences : HESS}, volume = {24}, journal = {Hydrology and earth system sciences : HESS}, number = {7}, publisher = {European Geosciences Union (EGU) ; Copernicus}, address = {Munich}, issn = {1027-5606}, doi = {10.5194/hess-24-3871-2020}, pages = {3871 -- 3880}, year = {2020}, abstract = {Biogenic greenhouse gas emissions, e.g., of methane (CH4) and carbon dioxide (CO2) from inland waters, contribute substantially to global warming. In aquatic systems, dissolved greenhouse gases are highly heterogeneous in both space and time. To better understand the biological and physical processes that affect sources and sinks of both CH4 and CO2, their dissolved concentrations need to be measured with high spatial and temporal resolution. To achieve this goal, we developed the Fast-Response Automated Gas Equilibrator (FaRAGE) for real-time in situ measurement of dissolved CH4 and CO2 concentrations at the water surface and in the water column. FaRAGE can achieve an exceptionally short response time (t(95\%) = 12 s when including the response time of the gas analyzer) while retaining an equilibration ratio of 62.6\% and a measurement accuracy of 0.5\% for CH4. A similar performance was observed for dissolved CO2 (t(95\%) = 10 s, equilibration ratio 67.1 \%). An equilibration ratio as high as 91.8\% can be reached at the cost of a slightly increased response time (16 s). The FaRAGE is capable of continuously measuring dissolved CO2 and CH4 concentrations in the nM-to-submM (10(-9)-10(-3) mol L-1) range with a detection limit of subnM (10(-10) mol L-1), when coupling with a cavity ring-down greenhouse gas analyzer (Picarro GasScouter). FaRAGE allows for the possibility of mapping dissolved concentration in a "quasi" three-dimensional manner in lakes and provides an inexpensive alternative to other commercial gas equilibrators. It is simple to operate and suitable for continuous monitoring with a strong tolerance for suspended particles. While the FaRAGE is developed for inland waters, it can be also applied to ocean waters by tuning the gas-water mixing ratio. The FaRAGE is easily adapted to suit other gas analyzers expanding the range of potential applications, including nitrous oxide and isotopic composition of the gases.}, language = {en} } @article{HokeWoodhouseZoccaratoetal.2020, author = {Hoke, Alexa and Woodhouse, Jason Nicholas and Zoccarato, Luca and McCarthy, Valerie and de Eyto, Elvira and Caldero-Pascual, Maria and Geffroy, Ewan and Dillane, Mary and Grossart, Hans-Peter and Jennings, Eleanor}, title = {Impacts of extreme weather events on bacterial community composition of a temperate humic lake}, series = {Water}, volume = {12}, journal = {Water}, number = {10}, publisher = {MDPI}, address = {Basel}, issn = {2073-4441}, doi = {10.3390/w12102757}, pages = {19}, year = {2020}, abstract = {Extreme weather events are projected to increase in frequency and intensity as climate change continues. Heterotrophic bacteria play a critical role in lake ecosystems, yet little research has been done to determine how they are affected by such extremes. The purpose of this study was to use high-throughput sequencing to explore the bacterial community composition of a humic oligotrophic lake on the North Atlantic Irish coast and to assess the impacts on composition dynamics related to extreme weather events. Samples for sequencing were collected from Lough Feeagh on a fortnightly basis from April to November 2018. Filtration was used to separate free-living and particle-associated bacterial communities and amplicon sequencing was performed for the 16S rRNA V4 region. Two named storms, six high discharge events, and one drought period occurred during the sampling period. These events had variable, context-dependent effects on bacterial communities in Lough Feeagh. The particle-associated community was found to be more likely to respond to physical changes, such as mixing, while the free-living population responded to changes in nutrient and carbon concentrations. Generally, however, the high stability of the bacterial community observed in Lough Feeagh suggests that the bacterial community is relatively resilient to extreme weather events.}, language = {en} } @article{SchellenbergReichertHardtetal.2020, author = {Schellenberg, Johannes and Reichert, Jessica and Hardt, Martin and Klingelh{\"o}fer, Ines and Morlock, Gertrud and Schubert, Patrick and Bižić, Mina and Grossart, Hans-Peter and K{\"a}mpfer, Peter and Wilke, Thomas and Glaeser, Stefanie P.}, title = {The bacterial microbiome of the long-term aquarium cultured high-microbial abundance sponge Haliclona cnidata}, series = {Frontiers in Marine Science}, volume = {7}, journal = {Frontiers in Marine Science}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2296-7745}, doi = {10.3389/fmars.2020.00266}, pages = {20}, year = {2020}, abstract = {Marine sponges host highly diverse but specific bacterial communities that provide essential functions for the sponge holobiont, including antimicrobial defense. Here, we characterized the bacterial microbiome of the marine sponge Haliclona cnidata that has been in culture in an artificial marine aquarium system. We tested the hypotheses (1) that the long-term aquarium cultured sponge H. cnidata is tightly associated with a typical sponge bacterial microbiota and (2) that the symbiotic Bacteria sustain bioactivity under harmful environmental conditions to facilitate holobiont survival by preventing pathogen invasion. Microscopic and phylogenetic analyses of the bacterial microbiota revealed that H. cnidata represents a high microbial abundance (HMA) sponge with a temporally stable bacterial community that significantly shifts with changing aquarium conditions. A 4-week incubation experiment was performed in small closed aquarium systems with antibiotic and/or light exclusion treatments to reduce the total bacterial and photosynthetically active sponge-associated microbiota to a treatment-specific resilient community. While the holobiont was severely affected by the experimental treatment (i.e., bleaching of the sponge, reduced bacterial abundance, shifted bacterial community composition), the biological defense and bacterial community interactions (i.e., quorum sensing activity) remained intact. 16S rRNA gene amplicon sequencing revealed a resilient community of 105 bacterial taxa, which remained in the treated sponges. These 105 taxa accounted for a relative abundance of 72-83\% of the bacterial sponge microbiota of non-treated sponge fragments that have been cultured under the same conditions. We conclude that a sponge-specific resilient community stays biologically active under harmful environmental conditions, facilitating the resilience of the holobiont. In H. cnidata, bacteria are located in bacteriocytes, which may have contributed to the observed phenomenon.}, language = {en} } @article{HuangQiaoXuetal.2021, author = {Huang, Lixing and Qiao, Ying and Xu, Wei and Gong, Linfeng and He, Rongchao and Qi, Weilu and Gao, Qiancheng and Cai, Hongyan and Grossart, Hans-Peter and Yan, Qingpi}, title = {Full-length transcriptome}, series = {Frontiers in immunology}, volume = {12}, journal = {Frontiers in immunology}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {1664-3224}, doi = {10.3389/fimmu.2021.737332}, pages = {18}, year = {2021}, abstract = {Fish is considered as a supreme model for clarifying the evolution and regulatory mechanism of vertebrate immunity. However, the knowledge of distinct immune cell populations in fish is still limited, and further development of techniques advancing the identification of fish immune cell populations and their functions are required. Single cell RNA-seq (scRNA-seq) has provided a new approach for effective in-depth identification and characterization of cell subpopulations. Current approaches for scRNA-seq data analysis usually rely on comparison with a reference genome and hence are not suited for samples without any reference genome, which is currently very common in fish research. Here, we present an alternative, i.e. scRNA-seq data analysis with a full-length transcriptome as a reference, and evaluate this approach on samples from Epinephelus coioides-a teleost without any published genome. We show that it reconstructs well most of the present transcripts in the scRNA-seq data achieving a sensitivity equivalent to approaches relying on genome alignments of related species. Based on cell heterogeneity and known markers, we characterized four cell types: T cells, B cells, monocytes/macrophages (Mo/M phi) and NCC (non-specific cytotoxic cells). Further analysis indicated the presence of two subsets of Mo/M phi including M1 and M2 type, as well as four subsets in B cells, i.e. mature B cells, immature B cells, pre B cells and early-pre B cells. Our research will provide new clues for understanding biological characteristics, development and function of immune cell populations of teleost. Furthermore, our approach provides a reliable alternative for scRNA-seq data analysis in teleost for which no reference genome is currently available.}, language = {en} } @article{AichnerDubbertKieletal.2022, author = {Aichner, Bernhard and Dubbert, David and Kiel, Christine and Kohnert, Katrin and Ogashawara, Igor and Jechow, Andreas and Harpenslager, Sarah-Faye and H{\"o}lker, Franz and Nejstgaard, Jens Christian and Grossart, Hans-Peter and Singer, Gabriel and Wollrab, Sabine and Berger, Stella Angela}, title = {Spatial and seasonal patterns of water isotopes in northeastern German lakes}, series = {Earth system science data : ESSD}, volume = {14}, journal = {Earth system science data : ESSD}, number = {4}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1866-3508}, doi = {10.5194/essd-14-1857-2022}, pages = {1857 -- 1867}, year = {2022}, abstract = {Water stable isotopes (delta O-18 and delta H-2) were analyzed in samples collected in lakes, associated with riverine systems in northeastern Germany, throughout 2020. The dataset (Aichner et al., 2021; https://doi.org/10.1594/PANGAEA.935633) is derived from water samples collected at (a) lake shores (sampled in March and July 2020), (b) buoys which were temporarily installed in deep parts of the lake (sampled monthly from March to October 2020), (c) multiple spatially distributed spots in four selected lakes (in September 2020), and (d) the outflow of Muggelsee (sampled biweekly from March 2020 to January 2021). At shores, water was sampled with a pipette from 40-60 cm below the water surface and directly transferred into a measurement vial, while at buoys a Limnos water sampler was used to obtain samples from 1 m below the surface. Isotope analysis was conducted at IGB Berlin, using a Picarro L2130-i cavity ring-down spectrometer, with a measurement uncertainty of < 0.15 parts per thousand (delta O-18) and < 0.0 parts per thousand (delta H-2). The data give information about the vegetation period and the full seasonal isotope amplitude in the sampled lakes and about spatial isotope variability in different branches of the associated riverine systems.}, language = {en} } @article{IlicicGrossart2022, author = {Ilicic, Doris and Grossart, Hans-Peter}, title = {Basal parasitic fungi in marine food webs-a mystery yet to unravel}, series = {Journal of Fungi}, volume = {8}, journal = {Journal of Fungi}, number = {2}, publisher = {MDPI}, address = {Basel}, issn = {2309-608X}, doi = {10.3390/jof8020114}, pages = {16}, year = {2022}, abstract = {Although aquatic and parasitic fungi have been well known for more than 100 years, they have only recently received increased awareness due to their key roles in microbial food webs and biogeochemical cycles. There is growing evidence indicating that fungi inhabit a wide range of marine habitats, from the deep sea all the way to surface waters, and recent advances in molecular tools, in particular metagenome approaches, reveal that their diversity is much greater and their ecological roles more important than previously considered. Parasitism constitutes one of the most widespread ecological interactions in nature, occurring in almost all environments. Despite that, the diversity of fungal parasites, their ecological functions, and, in particular their interactions with other microorganisms remain largely speculative, unexplored and are often missing from current theoretical concepts in marine ecology and biogeochemistry. In this review, we summarize and discuss recent research avenues on parasitic fungi and their ecological potential in marine ecosystems, e.g., the fungal shunt, and emphasize the need for further research.}, language = {en} } @article{RiemannRahavPassowetal.2022, author = {Riemann, Lasse and Rahav, Eyal and Passow, Uta and Grossart, Hans-Peter and de Beer, Dirk and Klawonn, Isabell and Eichner, Meri and Benavides, Mar and Bar-Zeev, Edo}, title = {Planktonic aggregates as hotspots for heterotrophic diazotrophy: the plot thickens}, series = {Frontiers in microbiology}, volume = {13}, journal = {Frontiers in microbiology}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {1664-302X}, doi = {10.3389/fmicb.2022.875050}, pages = {9}, year = {2022}, abstract = {Biological dinitrogen (N-2) fixation is performed solely by specialized bacteria and archaea termed diazotrophs, introducing new reactive nitrogen into aquatic environments. Conventionally, phototrophic cyanobacteria are considered the major diazotrophs in aquatic environments. However, accumulating evidence indicates that diverse non-cyanobacterial diazotrophs (NCDs) inhabit a wide range of aquatic ecosystems, including temperate and polar latitudes, coastal environments and the deep ocean. NCDs are thus suspected to impact global nitrogen cycling decisively, yet their ecological and quantitative importance remain unknown. Here we review recent molecular and biogeochemical evidence demonstrating that pelagic NCDs inhabit and thrive especially on aggregates in diverse aquatic ecosystems. Aggregates are characterized by reduced-oxygen microzones, high C:N ratio (above Redfield) and high availability of labile carbon as compared to the ambient water. We argue that planktonic aggregates are important loci for energetically-expensive N-2 fixation by NCDs and propose a conceptual framework for aggregate-associated N-2 fixation. Future studies on aggregate-associated diazotrophy, using novel methodological approaches, are encouraged to address the ecological relevance of NCDs for nitrogen cycling in aquatic environments.}, language = {en} } @article{ZoccaratoSherMikietal.2022, author = {Zoccarato, Luca and Sher, Daniel and Miki, Takeshi and Segre, Daniel and Grossart, Hans-Peter}, title = {A comparative whole-genome approach identifies bacterial traits for marine microbial interactions}, series = {Communications biology}, volume = {5}, journal = {Communications biology}, number = {1}, publisher = {Springer Nature}, address = {Berlin}, issn = {2399-3642}, doi = {10.1038/s42003-022-03184-4}, pages = {13}, year = {2022}, abstract = {Luca Zoccarato, Daniel Sher et al. leverage publicly available bacterial genomes from marine and other environments to examine traits underlying microbial interactions. Their results provide a valuable resource to investigate clusters of functional and linked traits to better understand marine bacteria community assembly and dynamics. Microbial interactions shape the structure and function of microbial communities with profound consequences for biogeochemical cycles and ecosystem health. Yet, most interaction mechanisms are studied only in model systems and their prevalence is unknown. To systematically explore the functional and interaction potential of sequenced marine bacteria, we developed a trait-based approach, and applied it to 473 complete genomes (248 genera), representing a substantial fraction of marine microbial communities. We identified genome functional clusters (GFCs) which group bacterial taxa with common ecology and life history. Most GFCs revealed unique combinations of interaction traits, including the production of siderophores (10\% of genomes), phytohormones (3-8\%) and different B vitamins (57-70\%). Specific GFCs, comprising Alpha- and Gammaproteobacteria, displayed more interaction traits than expected by chance, and are thus predicted to preferentially interact synergistically and/or antagonistically with bacteria and phytoplankton. Linked trait clusters (LTCs) identify traits that may have evolved to act together (e.g., secretion systems, nitrogen metabolism regulation and B vitamin transporters), providing testable hypotheses for complex mechanisms of microbial interactions. Our approach translates multidimensional genomic information into an atlas of marine bacteria and their putative functions, relevant for understanding the fundamental rules that govern community assembly and dynamics.}, language = {en} } @misc{WachsWrightSittichaietal.2021, author = {Wachs, Sebastian and Wright, Michelle F. and Sittichai, Ruthaychonnee and Singh, Ritu and Biswal, Ramakrishna and Kim, Eun-mee and Yang, Soeun and G{\´a}mez-Guadix, Manuel and Almendros, Carmen and Flora, Katerina and Daskalou, Vassiliki and Maziridou, Evdoxia}, title = {Correction: Associations between witnessing and perpetrating online hate in eight countries: The Buffering Effects of Problem-Focused Coping.}, series = {International Journal Environmental Research and Public Health}, volume = {18}, journal = {International Journal Environmental Research and Public Health}, number = {3992}, publisher = {MDPI}, address = {Basel}, issn = {1660-4601}, doi = {10.3390/ijerph18052609}, pages = {1 -- 2}, year = {2021}, language = {en} } @misc{SanchezSanzLaudenbachWeissetal.2023, author = {Sanchez Sanz, Arturo and Laudenbach, Beno{\^i}t and Weiß, Adrian and Werner, Eva and Stachon, Markus and Anders, Friedrich and Barthel, Christian and Berrens, Dominik and Avalli, Andrea and Vandewalle, Alexander and Ferrara, Pasquale and Pohl, Patrik}, title = {Spring Issue}, series = {thersites}, volume = {2023}, journal = {thersites}, number = {16}, editor = {Amb{\"u}hl, Annemarie and Carl{\`a}-Uhink, Filippo and Rollinger, Christian and Walde, Christine}, issn = {2364-7612}, doi = {10.34679/thersites.vol16}, year = {2023}, language = {en} } @misc{KnoblochKraftPaessleretal.2023, author = {Knobloch, Eberhard and Kraft, Tobias and P{\"a}ßler, Ulrich and Ette, Ottmar and G{\"o}tz, Carmen and Schwarz, Ingo}, title = {HiN : Alexander von Humboldt im Netz}, volume = {XXIV}, number = {47}, editor = {Ette, Ottmar and Knobloch, Eberhard}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {2568-3543}, doi = {doi.org/10.18443/hinvol24iss472023}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-611678}, pages = {122}, year = {2023}, abstract = {-Eberhard Knobloch: Alexander von Humboldts unbekannter Briefwechsel mit Ludwig August von Buch -Tobias Kraft und Ulrich P{\"a}ßler: Das Ganze erfassen. Dem Alexander-von-Humboldt-Forscher Eberhard Knobloch zum 80. Geburtstag -Ottmar Ette: Vor und nach der „gl{\"u}cklichen Revolution". Langsdorff, die Berliner Debatte um die Neue Welt und ihre Folgen f{\"u}r die wissenschaftlichen Expeditionen -Carmen G{\"o}tz: Die (Un-)Ordnung des Schreibens. Der Index g{\´e}n{\´e}ral und die Amerikanischen Reisetageb{\"u}cher -Ingo Schwarz: „Jedes ernste wissenschaftliche Streben ist ehrenwert." Carl Friedrich von Kl{\"o}dens Rezension des Kosmos von Alexander von Humboldt (1845) -Tobias Kraft und Ulrich P{\"a}ßler: Schriftenverzeichnis: Publikationen von Eberhard Knobloch zu Alexander von Humboldt -Eberhard Knobloch: Alexander von Humboldts Naturgem{\"a}lde der Anden}, language = {de} } @article{InceogluShpritsHeinemannetal.2022, author = {Inceoglu, Fadil and Shprits, Yuri and Heinemann, Stephan G. and Bianco, Stefano}, title = {Identification of coronal holes on AIA/SDO images using unsupervised machine learning}, series = {The astrophysical journal : an international review of spectroscopy and astronomical physics}, volume = {930}, journal = {The astrophysical journal : an international review of spectroscopy and astronomical physics}, number = {2}, publisher = {IOP Publ. Ltd.}, address = {Bristol}, issn = {1538-4357}, doi = {10.3847/1538-4357/ac5f43}, pages = {11}, year = {2022}, abstract = {Through its magnetic activity, the Sun governs the conditions in Earth's vicinity, creating space weather events, which have drastic effects on our space- and ground-based technology. One of the most important solar magnetic features creating the space weather is the solar wind that originates from the coronal holes (CHs). The identification of the CHs on the Sun as one of the source regions of the solar wind is therefore crucial to achieve predictive capabilities. In this study, we used an unsupervised machine-learning method, k-means, to pixel-wise cluster the passband images of the Sun taken by the Atmospheric Imaging Assembly on the Solar Dynamics Observatory in 171, 193, and 211 angstrom in different combinations. Our results show that the pixel-wise k-means clustering together with systematic pre- and postprocessing steps provides compatible results with those from complex methods, such as convolutional neural networks. More importantly, our study shows that there is a need for a CH database where a consensus about the CH boundaries is reached by observers independently. This database then can be used as the "ground truth," when using a supervised method or just to evaluate the goodness of the models.}, language = {en} } @article{SmirnovKronbergDalyetal.2020, author = {Smirnov, Artem G. and Kronberg, Elena A. and Daly, Patrick W. and Aseev, Nikita and Shprits, Yuri and Kellerman, Adam C.}, title = {Adiabatic Invariants Calculations for Cluster Mission: A Long-Term Product for Radiation Belts Studies}, series = {Journal of Geophysical Research: Space Physics}, volume = {125}, journal = {Journal of Geophysical Research: Space Physics}, number = {2}, publisher = {John Wiley \& Sons, Inc.}, address = {New Jersey}, pages = {12}, year = {2020}, abstract = {The Cluster mission has produced a large data set of electron flux measurements in the Earth's magnetosphere since its launch in late 2000. Electron fluxes are measured using Research with Adaptive Particle Imaging Detector (RAPID)/Imaging Electron Spectrometer (IES) detector as a function of energy, pitch angle, spacecraft position, and time. However, no adiabatic invariants have been calculated for Cluster so far. In this paper we present a step-by-step guide to calculations of adiabatic invariants and conversion of the electron flux to phase space density (PSD) in these coordinates. The electron flux is measured in two RAPID/IES energy channels providing pitch angle distribution at energies 39.2-50.5 and 68.1-94.5 keV in nominal mode since 2004. A fitting method allows to expand the conversion of the differential fluxes to the range from 40 to 150 keV. Best data coverage for phase space density in adiabatic invariant coordinates can be obtained for values of second adiabatic invariant, K, similar to 10(2), and values of the first adiabatic invariant mu in the range approximate to 5-20 MeV/G. Furthermore, we describe the production of a new data product "LSTAR," equivalent to the third adiabatic invariant, available through the Cluster Science Archive for years 2001-2018 with 1-min resolution. The produced data set adds to the availability of observations in Earth's radiation belts region and can be used for long-term statistical purposes.}, language = {en} } @article{Kuhlman2024, author = {Kuhlman, Sabine}, title = {Back to bureaucracy?}, series = {Journal of policy studies}, volume = {39}, journal = {Journal of policy studies}, number = {2}, publisher = {Graduate School of Public Administration}, address = {Seoul}, issn = {2799-9130}, doi = {10.52372/jps39202}, pages = {11 -- 44}, year = {2024}, abstract = {In this contribution, the emergence of the neo-Weberian state (NWS) is analyzed with regard to German public administration. Drawing on the concept of a governance space, which consists of a hierarchy, markets, and networks, we distinguish between four empirical manifestations of the NWS, namely, the NWS as (1) come back of the public/ re-municipalization; (2) re-hierarchization; (3) de-agencification; (4) de-escalation in performance management. These movements can, on the one hand, be interpreted as a (partial) reversal of New Public Management (NPM) approaches and a "swinging back of the pendulum" (see Kuhlmann \& Wollmann, 2019) toward public and classical Weberian principles (e.g., hierarchy, regulation, institutional re-aggregation). This reversal re-strengthened the hierarchy within the overall governance space to the detriment of, but without completely replacing, market mechanisms and networks. NPM's failure to deliver what it promised and its inappropriateness as a response to more recent challenges connected to crises and wicked problems have engendered a partial return of the public and a move away from the economization logic of NPM. On the other hand, post-NPM reversals and managerial de-escalation gave rise to hybrid models that merge NPM and classic Weberian administration. While some well-functioning combinations of NPM and Weberianism exist, the hybridization of "old" and "neo" elements has also provoked ambivalent and negative assessments regarding the actual functioning of the NWS in Germany. Our analysis suggests that the NWS is only partially suitable as a model for reform and future administrative modernization, largely depending on the context surrounding reform and implementation practices.}, language = {en} } @article{AsanteOseiAbdulSamedetal.2022, author = {Asante, Drusilla Obenewaa and Osei, Francis and Abdul-Samed, Fridaus and Nanevie, Victoria Dzifa}, title = {Knowledge and participation in exercise and physical activity among pregnant women in Ho, Ghana}, series = {Frontiers in Public Health}, volume = {10}, journal = {Frontiers in Public Health}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2296-2565}, doi = {10.3389/fpubh.2022.927191}, pages = {9}, year = {2022}, abstract = {Background Physical activity (PA) and exercise have been identified to improve the general fitness and health. Although, the Pregnancy Physical Activity Questionnaire (PPAQ) has been validated for use in assessing PA in pregnant women. However, understanding the knowledge and participation levels of PA in pregnant women in the underdeveloped regions of Ghana is of clinical relevance to foster education and promotion of PA. In Ghana, pregnant women believe the "myth" (mostly in rural areas and underdeveloped regions) that exercising in the first trimester might lead to miscarriage. Thus, the main objective of this study was to investigate the extent of knowledge and participation levels in PA among pregnant women in Ho, Ghana using a self-developed questionnaire which consisted of some questions adapted from the PPAQ. MethodsSeventy-seven (n = 77) pregnant women between the ages of 18-50 years were recruited from three hospitals across the Ho municipality of Ghana. A self-developed questionnaire which consisted of some questions taken from the PPAQ was administered to participants under the researchers' supervision. Spearman's correlation analysis was used to find the association between the level of participation in PA, knowledge of PA and gestational age among pregnant women. Results From the total participants (n = 77) recruited, 57 (74\%) of the participants scored high in PA knowledge. Most of the participants 48 (62.3\%) answered that PA promotes healthy pregnancy. Participants who reported barriers to PA during pregnancy were no exercise habits 51 (66.2\%), having no time 17 (22.1\%) and fear of miscarriage 9 (11.7\%). There was a significant (p < 0.05) association between the level of participation and gestational age. No significant (p > 0.05) association between the level of participation and knowledge of PA was observed. Conclusion There is a high level of knowledge of PA among pregnant women in Ho, Ghana. However, most pregnant women rather engage in PA as their gestational age increases. Thus, to foster sustainable exercise participation during pregnancy, all healthcare providers saddled with the responsibility of providing maternal healthcare must strengthen the education and promotion of exercise and PA among pregnant women in Ho, Ghana.}, language = {en} } @article{vonSteinkellerGrosse2022, author = {von Steinkeller, Annika and Grosse, Gerlind}, title = {Children are more social when playing analog games together than digital games}, series = {Computers in Human Behavior Reports}, volume = {6}, journal = {Computers in Human Behavior Reports}, publisher = {Elsevier}, address = {Amsterdam}, issn = {2451-9588}, doi = {10.1016/j.chbr.2022.100195}, pages = {10}, year = {2022}, abstract = {Digital media are being used more and more frequently by children and for a wide variety of functions. However, there are no studies to date that examine the effect of such use on peer interactions and the occurrence of prosocial behavior in peers. For parents, it has been found that when using digital media only few parents respond responsively to their children's attempts at interaction and also very rarely, they communicate with them verbally and nonverbally. In the present study, we investigated how playing a game in a digital versus analog form influences in-teractions (especially prosocial behavior) of peers. We used an experimental situation, where 24 dyads of 4-10-year-old children were examined. Each of the dyads was randomly assigned to a condition where they played either a digital or analog game together. Various interaction parameters and prosocial behavior during and after the game were analyzed. Results show that children in the analog condition communicated verbally with each other more often, responded more often to interaction attempts of their partners and showed less often negative forms of inter-action and more often positive forms of interaction than children in the digital condition. However, the type of medium had no influence on prosocial behavior after the game situation. These results suggest that the format of a game (digital vs. analog) has a decisive influence on peer interactions concerning their communication during but not their prosocial behavior after the game situation.}, language = {en} } @article{WinkelbeinerWandtEbertetal.2020, author = {Winkelbeiner, Nicola Lisa and Wandt, Viktoria Klara Veronika and Ebert, Franziska and Lossow, Kristina and Bankoglu, Ezgi E. and Martin, Maximilian and Mangerich, Aswin and Stopper, Helga and Bornhorst, Julia and Kipp, Anna Patricia and Schwerdtle, Tanja}, title = {A Multi-Endpoint Approach to Base Excision Repair Incision Activity Augmented by PARylation and DNA Damage Levels in Mice}, series = {International Journal of Molecular Sciences}, volume = {21}, journal = {International Journal of Molecular Sciences}, number = {18}, publisher = {Molecular Diversity Preservation International}, address = {Basel}, issn = {1422-0067}, doi = {10.3390/ijms21186600}, pages = {19}, year = {2020}, abstract = {Investigation of processes that contribute to the maintenance of genomic stability is one crucial factor in the attempt to understand mechanisms that facilitate ageing. The DNA damage response (DDR) and DNA repair mechanisms are crucial to safeguard the integrity of DNA and to prevent accumulation of persistent DNA damage. Among them, base excision repair (BER) plays a decisive role. BER is the major repair pathway for small oxidative base modifications and apurinic/apyrimidinic (AP) sites. We established a highly sensitive non-radioactive assay to measure BER incision activity in murine liver samples. Incision activity can be assessed towards the three DNA lesions 8-oxo-2'-deoxyguanosine (8-oxodG), 5-hydroxy-2'-deoxyuracil (5-OHdU), and an AP site analogue. We applied the established assay to murine livers of adult and old mice of both sexes. Furthermore, poly(ADP-ribosyl)ation (PARylation) was assessed, which is an important determinant in DDR and BER. Additionally, DNA damage levels were measured to examine the overall damage levels. No impact of ageing on the investigated endpoints in liver tissue were found. However, animal sex seems to be a significant impact factor, as evident by sex-dependent alterations in all endpoints investigated. Moreover, our results revealed interrelationships between the investigated endpoints indicative for the synergetic mode of action of the cellular DNA integrity maintaining machinery.}, language = {en} } @article{SchmidtFelisattiAsteretal.2021, author = {Schmidt, Hendrikje and Felisatti, Arianna and Aster, Michael von and Wilbert, J{\"u}rgen and Moers, Arpad von and Fischer, Martin H.}, title = {Neuromuscular diseases affect number representation and processing}, series = {Frontiers in psychology / Frontiers Research Foundation}, volume = {12}, journal = {Frontiers in psychology / Frontiers Research Foundation}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2021.697881}, pages = {13}, year = {2021}, abstract = {Spinal muscular atrophy (SMA) and Duchenne muscular dystrophy (DMD) both are rare genetic neuromuscular diseases with progressive loss of motor ability. The neuromotor developmental course of those diseases is well documented. In contrast, there is only little evidence about characteristics of general and specific cognitive development. In both conditions the final motor outcome is characterized by an inability to move autonomously: children with SMA never accomplish independent motoric exploration of their environment, while children with DMD do but later lose this ability again. These profound differences in developmental pathways might affect cognitive development of SMA vs. DMD children, as cognition is shaped by individual motor experiences. DMD patients show impaired executive functions, working memory, and verbal IQ, whereas only motor ability seems to be impaired in SMA. Advanced cognitive capacity in SMA may serve as a compensatory mechanism for achieving in education, career progression, and social satisfaction. This study aimed to relate differences in basic numerical concepts and arithmetic achievement in SMA and DMD patients to differences in their motor development and resulting sensorimotor and environmental experiences. Horizontal and vertical spatial-numerical associations were explored in SMA/DMD children ranging between 6 and 12 years through the random number generation task. Furthermore, arithmetic skills as well as general cognitive ability were assessed. Groups differed in spatial number processing as well as in arithmetic and domain-general cognitive functions. Children with SMA showed no horizontal and even reversed vertical spatial-numerical associations. Children with DMD on the other hand revealed patterns in spatial numerical associations comparable to healthy developing children. From the embodied Cognition perspective, early sensorimotor experience does play a role in development of mental number representations. However, it remains open whether and how this becomes relevant for the acquisition of higher order cognitive and arithmetic skills.}, language = {en} } @article{RichlyBrauerSchlosser2020, author = {Richly, Keven and Brauer, Janos and Schlosser, Rainer}, title = {Predicting location probabilities of drivers to improved dispatch decisions of transportation network companies based on trajectory data}, series = {Proceedings of the 9th International Conference on Operations Research and Enterprise Systems - ICORES}, journal = {Proceedings of the 9th International Conference on Operations Research and Enterprise Systems - ICORES}, publisher = {Springer}, address = {Berlin}, pages = {12}, year = {2020}, abstract = {The demand for peer-to-peer ridesharing services increased over the last years rapidly. To cost-efficiently dispatch orders and communicate accurate pick-up times is challenging as the current location of each available driver is not exactly known since observed locations can be outdated for several seconds. The developed trajectory visualization tool enables transportation network companies to analyze dispatch processes and determine the causes of unexpected delays. As dispatching algorithms are based on the accuracy of arrival time predictions, we account for factors like noise, sample rate, technical and economic limitations as well as the duration of the entire process as they have an impact on the accuracy of spatio-temporal data. To improve dispatching strategies, we propose a prediction approach that provides a probability distribution for a driver's future locations based on patterns observed in past trajectories. We demonstrate the capabilities of our prediction results to ( i) avoid critical delays, (ii) to estimate waiting times with higher confidence, and (iii) to enable risk considerations in dispatching strategies.}, language = {en} } @article{WagnerRosenbergHofmannetal.2020, author = {Wagner, Birgit and Rosenberg, Nicole and Hofmann, Laura and Maaß, Ulrike}, title = {Web-based bereavement care}, series = {Frontiers in psychiatry}, volume = {11}, journal = {Frontiers in psychiatry}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {1664-0640}, doi = {10.3389/fpsyt.2020.00525}, pages = {13}, year = {2020}, abstract = {Background: Web-based interventions have been introduced as novel and effective treatments for mental disorders and, in recent years, specifically for the bereaved. However, a systematic summary of the effectiveness of online interventions for people experiencing bereavement is still missing. Objective: A systematic literature search was conducted by four reviewers who reviewed and meta-analytically summarized the evidence for web-based interventions for bereaved people. Methods: Systematic searches (PubMed, Web of Science, PsycInfo, PsycArticles, Medline, and CINAHL) resulted in seven randomized controlled trials (N= 1,257) that addressed adults having experienced bereavement using internet-based interventions. We used random effects models to summarize treatment effects for between-group comparisons (treatmentvs.control at post) and stability over time (postvs.follow-up). Results: All web-based interventions were based on cognitive behavioral therapy (CBT). In comparison with control groups, the interventions showed moderate (g= .54) to large effects (g= .86) for symptoms of grief and posttraumatic stress disorder (PTSD), respectively. The effect for depression was small (g= .44). All effects were stable over time. A higher number of treatment sessions achieved higher effects for grief symptoms and more individual feedback increased effects for depression. Other moderators (i.e.dropout rate, time since loss, exposure) did not significantly reduce moderate degrees of heterogeneity between the studies. Limitations: The number of includable studies was low in this review resulting to lower power for moderator analyses in particular. Conclusions: Overall, the results of web-based bereavement interventions are promising, and its low-threshold approach might reduce barriers to bereavement care. Nonetheless, future research should further examine potential moderators and specific treatment components (e.g.exposure, feedback) and compare interventions with active controls.}, language = {en} } @article{WagnerHofmannMaass2020, author = {Wagner, Birgit and Hofmann, Laura and Maaß, Ulrike}, title = {Online-group intervention after suicide bereavement through the use of webinars}, series = {Trials}, volume = {21}, journal = {Trials}, number = {1}, publisher = {BioMed Central}, address = {London}, issn = {1468-6694}, doi = {10.1186/s13063-019-3891-5}, pages = {13}, year = {2020}, abstract = {Introduction: The death of a significant person through suicide is a very difficult experience and can have long-term impact on an individual's psychosocial and physical functioning. However, there are only few studies that have examined the effects of interventions in suicide survivors. In the present study, we examine an online-group intervention for people bereaved by suicide using a group-webinar. Methods: The intervention was developed based on focus groups with the target group. The cognitive-behavioral 12-module webinar-based group intervention focuses on suicide bereavement-related themes such as feelings of guilt, stigmatization, meaning reconstruction and the relationship to the deceased. Further, the webinar includes testimonial videos and psychoeducation. The suicide survivors are randomized to the intervention or the waiting list in a group-cluster randomized controlled trial. Primary outcomes are suicidality (Beck Scale for Suicide Ideation) and depression (Beck Depression Inventory-II) and secondary outcomes are symptoms of prolonged grief disorder (Inventory of Complicated Grief-German Version ), posttraumatic stress disorder ( Revised Impact of Event Scale ), stigmatization (Stigma of Suicide and Suicide Survivor ) and posttraumatic cognitions (Posttraumatic Cognitions Inventory). Discussion: Previous studies of Internet-based interventions for the bereaved were based on writing interventions showing large treatment effects. Little is known about the use of webinars as group interventions. Advantages and challenges of this novel approach of psychological interventions will be discussed.}, language = {en} } @article{MaassKuehneHeinzeetal.2022, author = {Maaß, Ulrike and K{\"u}hne, Franziska and Heinze, Peter Eric and Ay-Bryson, Destina Sevde and Weck, Florian}, title = {The concise measurement of clinical communication skills}, series = {Frontiers in Psychiatry}, volume = {13}, journal = {Frontiers in Psychiatry}, publisher = {Frontiers}, address = {Lausanne, Schweiz}, issn = {1664-0640}, doi = {10.3389/fpsyt.2022.977324}, pages = {10}, year = {2022}, abstract = {Objective: There is a lack of brief rating scales for the reliable assessment of psychotherapeutic skills, which do not require intensive rater training and/or a high level of expertise. Thus, the objective is to validate a 14-item version of the Clinical Communication Skills Scale (CCSS-S). Methods: Using a sample of N = 690 video-based ratings of role-plays with simulated patients, we calculated a confirmatory factor analysis and an exploratory structural equation modeling (ESEM), assessed convergent validities, determined inter-rater reliabilities and compared these with those who were either psychology students, advanced psychotherapy trainees, or experts. Results: Correlations with other competence rating scales were high (rs > 0.86-0.89). The intraclass correlations ranged between moderate and good [ICC(2,2) = 0.65-0.80], with student raters yielding the lowest scores. The one-factor model only marginally replicated the data, but the internal consistencies were excellent (α = 0.91-95). The ESEM yielded a two-factor solution (Collaboration and Structuring and Exploration Skills). Conclusion: The CCSS-S is a brief and valid rating scale that reliably assesses basic communication skills, which is particularly useful for psychotherapy training using standardized role-plays. To ensure good inter-rater reliabilities, it is still advisable to employ raters with at least some clinical experience. Future studies should further investigate the one- or two-factor structure of the instrument.}, language = {en} } @article{FelisattiAagtenMurphyLaubrocketal.2020, author = {Felisatti, Arianna and Aagten-Murphy, David and Laubrock, Jochen and Shaki, Samuel and Fischer, Martin H.}, title = {The brain's asymmetric frequency tuning}, series = {Symmetry / Molecular Diversity Preservation International (MDPI)}, volume = {12}, journal = {Symmetry / Molecular Diversity Preservation International (MDPI)}, number = {12}, publisher = {MDPI}, address = {Basel}, issn = {2073-8994}, doi = {10.3390/sym12122083}, pages = {25}, year = {2020}, abstract = {To construct a coherent multi-modal percept, vertebrate brains extract low-level features (such as spatial and temporal frequencies) from incoming sensory signals. However, because frequency processing is lateralized with the right hemisphere favouring low frequencies while the left favours higher frequencies, this introduces asymmetries between the hemispheres. Here, we describe how this lateralization shapes the development of several cognitive domains, ranging from visuo-spatial and numerical cognition to language, social cognition, and even aesthetic appreciation, and leads to the emergence of asymmetries in behaviour. We discuss the neuropsychological and educational implications of these emergent asymmetries and suggest future research approaches.}, language = {en} } @article{HeistermannFranckeScheiffeleetal.2023, author = {Heistermann, Maik and Francke, Till and Scheiffele, Lena and Petrova, Katya Dimitrova and Budach, Christian and Schr{\"o}n, Martin and Trost, Benjamin and Rasche, Daniel and G{\"u}ntner, Andreas and Doepper, Veronika and F{\"o}rster, Michael and K{\"o}hli, Markus and Angermann, Lisa and Antonoglou, Nikolaos and Zude, Manuela and Oswald, Sascha}, title = {Three years of soil moisture observations by a dense cosmic-ray neutron sensing cluster at an agricultural research site in north-east Germany}, series = {Earth system science data : ESSD}, volume = {15}, journal = {Earth system science data : ESSD}, number = {7}, publisher = {Copernics Publications}, address = {Katlenburg-Lindau}, issn = {1866-3508}, doi = {10.5194/essd-15-3243-2023}, pages = {3243 -- 3262}, year = {2023}, abstract = {Cosmic-ray neutron sensing (CRNS) allows for the estimation of root-zone soil water content (SWC) at the scale of several hectares. In this paper, we present the data recorded by a dense CRNS network operated from 2019 to 2022 at an agricultural research site in Marquardt, Germany - the first multi-year CRNS cluster. Consisting, at its core, of eight permanently installed CRNS sensors, the cluster was supplemented by a wealth of complementary measurements: data from seven additional temporary CRNS sensors, partly co-located with the permanent ones; 27 SWC profiles (mostly permanent); two groundwater observation wells; meteorological records; and Global Navigation Satellite System reflectometry (GNSS-R). Complementary to these continuous measurements, numerous campaign-based activities provided data by mobile CRNS roving, hyperspectral im-agery via UASs, intensive manual sampling of soil properties (SWC, bulk density, organic matter, texture, soil hydraulic properties), and observations of biomass and snow (cover, depth, and density). The unique temporal coverage of 3 years entails a broad spectrum of hydro-meteorological conditions, including exceptional drought periods and extreme rainfall but also episodes of snow coverage, as well as a dedicated irrigation experiment. Apart from serving to advance CRNS-related retrieval methods, this data set is expected to be useful for vari-ous disciplines, for example, soil and groundwater hydrology, agriculture, or remote sensing. Hence, we show exemplary features of the data set in order to highlight the potential for such subsequent studies. The data are available at doi.org/10.23728/b2share.551095325d74431881185fba1eb09c95 (Heistermann et al., 2022b).}, language = {en} } @book{PuergstallerNeuber2024, author = {P{\"u}rgstaller, Esther and Neuber, Nils}, title = {T{\"a}nzerischer Kreativit{\"a}tstest f{\"u}r 8- bis 12-J{\"a}hrige}, editor = {P{\"u}rgstaller, Esther and Neuber, Nils}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, doi = {10.25932/publishup-62704}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-627040}, publisher = {Universit{\"a}t Potsdam}, pages = {83}, year = {2024}, abstract = {Der t{\"a}nzerische Kreativit{\"a}tstest stellt ein valides Instrumentarium dar, welches auf tanzspezifischen Aufgaben basiert und f{\"u}r die differenzierte und standardisierte Erfassung der t{\"a}nzerischen Kreativit{\"a}t bei Kindern im Alter von 8 bis 12 Jahren konzipiert ist. Mit dem t{\"a}nzerischen Kreativit{\"a}tstest k{\"o}nnen nicht nur Fragestellungen zum Stand sowie zur Entwicklung t{\"a}nzerisch-kreativer F{\"a}higkeiten im Kindesalter bearbeitet werden, sondern er liefert auch wertvolle Informationen f{\"u}r die Optimierung von Trainings-, F{\"o}rder- und Vermittlungsmaßnahmen. Erfasst werden folgende t{\"a}nzerisch-kreativen F{\"a}higkeiten: 1) Vielfalt und Originalit{\"a}t in der Fortbewegung und in K{\"o}rperpositionen sowie 2) Ideenreichtum, Vielfalt und Originalit{\"a}t in der Gestaltung von Bewegungspatterns und -kompositionen. Dieser Test l{\"a}sst sich mit gr{\"o}ßeren Gruppen und minimalem materiellen Aufwand durchf{\"u}hren, ist zeitlich unbeschr{\"a}nkt und erm{\"o}glicht es, unterschiedliche Leistungsniveaus zu identifizieren. Der t{\"a}nzerische Kreativit{\"a}tstest bietet Forschenden und Lehrkr{\"a}ften eine wertvolle M{\"o}glichkeit, die t{\"a}nzerisch-kreativen F{\"a}higkeiten von Kindern zu analysieren und zu f{\"o}rdern.}, language = {de} } @article{SmirnovShpritsAllisonetal.2022, author = {Smirnov, Artem and Shprits, Yuri and Allison, Hayley and Aseev, Nikita and Drozdov, Alexander and Kollmann, Peter and Wang, Dedong and Saikin, Anthony}, title = {Storm-Time evolution of the Equatorial Electron Pitch Angle Distributions in Earth's Outer Radiation Belt}, series = {Frontiers in astronomy and space sciences}, volume = {9}, journal = {Frontiers in astronomy and space sciences}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2296-987X}, doi = {10.3389/fspas.2022.836811}, pages = {15}, year = {2022}, abstract = {In this study we analyze the storm-time evolution of equatorial electron pitch angle distributions (PADs) in the outer radiation belt region using observations from the Magnetic Electron Ion Spectrometer (MagEIS) instrument aboard the Van Allen Probes in 2012-2019. The PADs are approximated using a sum of the first, third and fifth sine harmonics. Different combinations of the respective coefficients refer to the main PAD shapes within the outer radiation belt, namely the pancake, flat-top, butterfly and cap PADs. We conduct a superposed epoch analysis of 129 geomagnetic storms and analyze the PAD evolution for day and night MLT sectors. PAD shapes exhibit a strong energy-dependent response. At energies of tens of keV, the PADs exhibit little variation throughout geomagnetic storms. Cap PADs are mainly observed at energies < 300 keV, and their extent in L shrinks with increasing energy. The cap distributions transform into the pancake PADs around the main phase of the storm on the nightside, and then come back to their original shapes during the recovery phase. At higher energies on the dayside, the PADs are mainly pancake during pre-storm conditions and become more anisotropic during the main phase. The quiet-time butterfly PADs can be observed on the nightside at L> 5.6. During the main phase, butterfly PADs have stronger 90 degrees-minima and can be observed at lower L-shells (down to L = 5), then transitioning into flat-top PADs at L similar to 4.5 - 5 and pancake PADs at L < 4.5. The resulting PAD coefficients for different energies, locations and storm epochs can be used to test the wave models and physics-based radiation belt codes in terms of pitch angle distributions.}, language = {en} } @misc{DuevelEhmigMcCalletal.2024, author = {D{\"u}vel, Pia and Ehmig, Ulrike and McCall, Jeremiah and Unceta G{\´o}mez, Luis and Bakogianni, Anastasia and Fischer, Jens and Serrano Lozano, David and Amb{\"u}hl, Annemarie and Matz, Alicia and Brinker, Wolfram and Mach, Jonas Konstantin and Mancini, Mattia and Werner, Eva}, title = {Spring Issue}, series = {thersites}, volume = {2024}, journal = {thersites}, number = {18}, editor = {Amb{\"u}hl, Annemarie and Carl{\`a}-Uhink, Filippo and Rollinger, Christian and Walde, Christine}, issn = {2364-7612}, doi = {10.34679/thersites.vol18}, year = {2024}, language = {en} } @article{ShpritsMeniettiDrozdovetal.2018, author = {Shprits, Yuri and Menietti, J. D. and Drozdov, Alexander and Horne, Richard B. and Woodfield, Emma E. and Groene, J. B. and de Soria-Santacruz, M. and Averkamp, T. F. and Garrett, H. and Paranicas, C. and Gurnett, Don A.}, title = {Strong whistler mode waves observed in the vicinity of Jupiter's moons}, series = {Nature Communications}, volume = {9}, journal = {Nature Communications}, publisher = {Nature Publ. Group}, address = {London}, issn = {2041-1723}, doi = {10.1038/s41467-018-05431-x}, pages = {6}, year = {2018}, abstract = {Understanding of wave environments is critical for the understanding of how particles are accelerated and lost in space. This study shows that in the vicinity of Europa and Ganymede, that respectively have induced and internal magnetic fields, chorus wave power is significantly increased. The observed enhancements are persistent and exceed median values of wave activity by up to 6 orders of magnitude for Ganymede. Produced waves may have a pronounced effect on the acceleration and loss of particles in the Jovian magnetosphere and other astrophysical objects. The generated waves are capable of significantly modifying the energetic particle environment, accelerating particles to very high energies, or producing depletions in phase space density. Observations of Jupiter's magnetosphere provide a unique opportunity to observe how objects with an internal magnetic field can interact with particles trapped in magnetic fields of larger scale objects.}, language = {en} } @article{AdolfsHoqueShprits2022, author = {Adolfs, Marjolijn and Hoque, Mohammed Mainul and Shprits, Yuri}, title = {Storm-time relative total electron content modelling using machine learning techniques}, series = {Remote sensing}, volume = {14}, journal = {Remote sensing}, number = {23}, publisher = {MDPI}, address = {Basel}, issn = {2072-4292}, doi = {10.3390/rs14236155}, pages = {17}, year = {2022}, abstract = {Accurately predicting total electron content (TEC) during geomagnetic storms is still a challenging task for ionospheric models. In this work, a neural-network (NN)-based model is proposed which predicts relative TEC with respect to the preceding 27-day median TEC, during storm time for the European region (with longitudes 30 degrees W-50 degrees E and latitudes 32.5 degrees N-70 degrees N). The 27-day median TEC (referred to as median TEC), latitude, longitude, universal time, storm time, solar radio flux index F10.7, global storm index SYM-H and geomagnetic activity index Hp30 are used as inputs and the output of the network is the relative TEC. The relative TEC can be converted to the actual TEC knowing the median TEC. The median TEC is calculated at each grid point over the European region considering data from the last 27 days before the storm using global ionosphere maps (GIMs) from international GNSS service (IGS) sources. A storm event is defined when the storm time disturbance index Dst drops below 50 nanotesla. The model was trained with storm-time relative TEC data from the time period of 1998 until 2019 (2015 is excluded) and contains 365 storms. Unseen storm data from 33 storm events during 2015 and 2020 were used to test the model. The UQRG GIMs were used because of their high temporal resolution (15 min) compared to other products from different analysis centers. The NN-based model predictions show the seasonal behavior of the storms including positive and negative storm phases during winter and summer, respectively, and show a mixture of both phases during equinoxes. The model's performance was also compared with the Neustrelitz TEC model (NTCM) and the NN-based quiet-time TEC model, both developed at the German Aerospace Agency (DLR). The storm model has a root mean squared error (RMSE) of 3.38 TEC units (TECU), which is an improvement by 1.87 TECU compared to the NTCM, where an RMSE of 5.25 TECU was found. This improvement corresponds to a performance increase by 35.6\%. The storm-time model outperforms the quiet-time model by 1.34 TECU, which corresponds to a performance increase by 28.4\% from 4.72 to 3.38 TECU. The quiet-time model was trained with Carrington averaged TEC and, therefore, is ideal to be used as an input instead of the GIM derived 27-day median. We found an improvement by 0.8 TECU which corresponds to a performance increase by 17\% from 4.72 to 3.92 TECU for the storm-time model using the quiet-time-model predicted TEC as an input compared to solely using the quiet-time model.}, language = {en} } @book{GarusSawahnWankeetal.2023, author = {Garus, Marcel and Sawahn, Rohan and Wanke, Jonas and Tiedt, Clemens and Granzow, Clara and Kuffner, Tim and Rosenbaum, Jannis and Hagemann, Linus and Wollnik, Tom and Woth, Lorenz and Auringer, Felix and Kantusch, Tobias and Roth, Felix and Hanff, Konrad and Schilli, Niklas and Seibold, Leonard and Lindner, Marc Fabian and Raschack, Selina}, title = {Operating systems II - student projects}, number = {142}, editor = {Grapentin, Andreas and Tiedt, Clemens and Polze, Andreas}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-524-8}, issn = {1613-5652}, doi = {10.25932/publishup-52636}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-526363}, publisher = {Universit{\"a}t Potsdam}, pages = {ix, 114}, year = {2023}, abstract = {This technical report presents the results of student projects which were prepared during the lecture "Operating Systems II" offered by the "Operating Systems and Middleware" group at HPI in the Summer term of 2020. The lecture covered ad- vanced aspects of operating system implementation and architecture on topics such as Virtualization, File Systems and Input/Output Systems. In addition to attending the lecture, the participating students were encouraged to gather practical experience by completing a project on a closely related topic over the course of the semester. The results of 10 selected exceptional projects are covered in this report. The students have completed hands-on projects on the topics of Operating System Design Concepts and Implementation, Hardware/Software Co-Design, Reverse Engineering, Quantum Computing, Static Source-Code Analysis, Operating Systems History, Application Binary Formats and more. It should be recognized that over the course of the semester all of these projects have achieved outstanding results which went far beyond the scope and the expec- tations of the lecture, and we would like to thank all participating students for their commitment and their effort in completing their respective projects, as well as their work on compiling this report.}, language = {en} } @book{MeinelGalbasHageboelling2023, author = {Meinel, Christoph and Galbas, Michael and Hageb{\"o}lling, David}, title = {Digitale Souver{\"a}nit{\"a}t: Erkenntnisse aus dem deutschen Bildungssektor}, number = {156}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-560-6}, issn = {1613-5652}, doi = {10.25932/publishup-59513}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-595138}, publisher = {Universit{\"a}t Potsdam}, pages = {1 -- 29}, year = {2023}, abstract = {Digitale Technologien bieten erhebliche politische, wirtschaftliche und gesellschaftliche Chancen. Zugleich ist der Begriff digitale Souver{\"a}nit{\"a}t zu einem Leitmotiv im deutschen Diskurs {\"u}ber digitale Technologien geworden: das heißt, die F{\"a}higkeit des Staates, seine Verantwortung wahrzunehmen und die Bef{\"a}higung der Gesellschaft - und des Einzelnen - sicherzustellen, die digitale Transformation selbstbestimmt zu gestalten. Exemplarisch f{\"u}r die Herausforderung in Deutschland und Europa, die Vorteile digitaler Technologien zu nutzen und gleichzeitig Souver{\"a}nit{\"a}tsbedenken zu ber{\"u}cksichtigen, steht der Bildungssektor. Er umfasst Bildung als zentrales {\"o}ffentliches Gut, ein schnell aufkommendes Gesch{\"a}ftsfeld und wachsende Best{\"a}nde an hochsensiblen personenbezogenen Daten. Davon ausgehend beschreibt der Bericht Wege zur Entsch{\"a}rfung des Spannungsverh{\"a}ltnisses zwischen Digitalisierung und Souver{\"a}nit{\"a}t auf drei verschiedenen Ebenen - Staat, Wirtschaft und Individuum - anhand konkreter technischer Projekte im Bildungsbereich: die HPI Schul-Cloud (staatliche Souver{\"a}nit{\"a}t), die MERLOT-Datenr{\"a}ume (wirtschaftliche Souver{\"a}nit{\"a}t) und die openHPI-Plattform (individuelle Souver{\"a}nit{\"a}t).}, language = {de} } @book{MeinelGalbasHageboelling2023, author = {Meinel, Christoph and Galbas, Michael and Hageb{\"o}lling, David}, title = {Digital sovereignty: insights from Germany's education sector}, number = {157}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-561-3}, issn = {1613-5652}, doi = {10.25932/publishup-59772}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-597723}, publisher = {Universit{\"a}t Potsdam}, pages = {1 -- 27}, year = {2023}, abstract = {Digital technology offers significant political, economic, and societal opportunities. At the same time, the notion of digital sovereignty has become a leitmotif in German discourse: the state's capacity to assume its responsibilities and safeguard society's - and individuals' - ability to shape the digital transformation in a self-determined way. The education sector is exemplary for the challenge faced by Germany, and indeed Europe, of harnessing the benefits of digital technology while navigating concerns around sovereignty. It encompasses education as a core public good, a rapidly growing field of business, and growing pools of highly sensitive personal data. The report describes pathways to mitigating the tension between digitalization and sovereignty at three different levels - state, economy, and individual - through the lens of concrete technical projects in the education sector: the HPI Schul-Cloud (state sovereignty), the MERLOT data spaces (economic sovereignty), and the openHPI platform (individual sovereignty).}, language = {en} } @article{ZwickelKahlRychliketal.2018, author = {Zwickel, Theresa and Kahl, Sandra and Rychlik, Michael and M{\"u}ller, Marina E. H.}, title = {Chemotaxonomy of Mycotoxigenic Small-Spored Alternaria Fungi}, series = {Frontiers in microbiology}, volume = {9}, journal = {Frontiers in microbiology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-302X}, doi = {10.3389/fmicb.2018.01368}, pages = {20}, year = {2018}, abstract = {Necrotrophic as well as saprophytic small-spored Altemaria (A.) species are annually responsible for major losses of agricultural products, such as cereal crops, associated with the contamination of food and feedstuff with potential health-endangering Altemaria toxins. Knowledge of the metabolic capabilities of different species-groups to form mycotoxins is of importance for a reliable risk assessment. 93 Altemaria strains belonging to the four species groups Alternaria tenuissima, A. arborescens, A. altemata, and A. infectoria were isolated from winter wheat kernels harvested from fields in Germany and Russia and incubated under equal conditions. Chemical analysis by means of an HPLC-MS/MS multi-Alternaria-toxin-method showed that 95\% of all strains were able to form at least one of the targeted 17 non-host specific Altemaria toxins. Simultaneous production of up to 15 (modified) Altemaria toxins by members of the A. tenuissima, A. arborescens, A. altemata species-groups and up to seven toxins by A. infectoria strains was demonstrated. Overall tenuazonic acid was the most extensively formed mycotoxin followed by alternariol and alternariol mono methylether, whereas altertoxin I was the most frequently detected toxin. Sulfoconjugated modifications of alternariol, alternariol mono methylether, altenuisol and altenuene were frequently determined. Unknown perylene quinone derivatives were additionally detected. Strains of the species-group A. infectoria could be segregated from strains of the other three species-groups due to significantly lower toxin levels and the specific production of infectopyrone. Apart from infectopyrone, alterperylenol was also frequently produced by 95\% of the A. infectoria strains. Neither by the concentration nor by the composition of the targeted Altemaria toxins a differentiation between the species-groups A. altemata, A. tenuissima and A. arborescens was possible.}, language = {en} } @misc{EtteSchwarzGaoetal.2024, author = {Ette, Ottmar and Schwarz, Ingo and Gao, Hong and Kraft, Tobias and Kutzinski, Vera and Zielnica, Krzysztof}, title = {HiN : Alexander von Humboldt im Netz}, volume = {XXV}, number = {48}, editor = {Ette, Ottmar and Knobloch, Eberhard}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {2568-3543}, doi = {10.18443/hinvol25iss482024}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-633524}, pages = {100}, year = {2024}, abstract = {-Ottmar Ette, Ingo Schwarz: „Ein junges, neues Geschlecht wird besseres liefern als das alte". Ein Empfehlungsbrief Alexander von Humboldts f{\"u}r Carl Ludwig -GAO Hong: Nachgedanken zur {\"U}bersetzung des ersten Bandes von Humboldts Kosmos -Tobias Kraft: Neue Quellen zu Humboldts Kuba-Forschung. Das „Digitale Dossier" des Proyecto Humboldt Digital (2019 - 2023) -Vera Kutzinski: Off-Road Adventures: Reading Statistics in Alexander von Humboldt's Political Essay on the Kingdom of New Spain -Krzysztof Zielnica: Alexander von Humboldt und Polen - zum 150. Jahrestag seiner Reise nach Warschau. Mit einleitenden Worten von Ingo Schwarz}, language = {de} } @article{LandisSaikinZhelavskayaetal.2022, author = {Landis, Daji August and Saikin, Anthony and Zhelavskaya, Irina and Drozdov, Alexander and Aseev, Nikita and Shprits, Yuri and Pfitzer, Maximilian F. and Smirnov, Artem G.}, title = {NARX Neural Network Derivations of the Outer Boundary Radiation Belt Electron Flux}, series = {Space Weather: the international journal of research and applications}, volume = {20}, journal = {Space Weather: the international journal of research and applications}, number = {5}, publisher = {American Geophysical Union}, address = {Washington}, issn = {1542-7390}, doi = {10.1029/2021SW002774}, pages = {18}, year = {2022}, abstract = {We present two new empirical models of radiation belt electron flux at geostationary orbit. GOES-15 measurements of 0.8 MeV electrons were used to train a Nonlinear Autoregressive with Exogenous input (NARX) neural network for both modeling GOES-15 flux values and an upper boundary condition scaling factor (BF). The GOES-15 flux model utilizes an input and feedback delay of 2 and 2 time steps (i.e., 5 min time steps) with the most efficient number of hidden layers set to 10. Magnetic local time, Dst, Kp, solar wind dynamic pressure, AE, and solar wind velocity were found to perform as predicative indicators of GOES-15 flux and therefore were used as the exogenous inputs. The NARX-derived upper boundary condition scaling factor was used in conjunction with the Versatile Electron Radiation Belt (VERB) code to produce reconstructions of the radiation belts during the period of July-November 1990, independent of in-situ observations. Here, Kp was chosen as the sole exogenous input to be more compatible with the VERB code. This Combined Release and Radiation Effects Satellite-era reconstruction showcases the potential to use these neural network-derived boundary conditions as a method of hindcasting the historical radiation belts. This study serves as a companion paper to another recently published study on reconstructing the radiation belts during Solar Cycles 17-24 (Saikin et al., 2021, ), for which the results featured in this paper were used.}, language = {en} } @article{ProlSmirnovHoqueetal.2022, author = {Prol, Fabricio S. and Smirnov, Artem G. and Hoque, M. Mainul and Shprits, Yuri}, title = {Combined model of topside ionosphere and plasmasphere derived from radio-occultation and Van Allen Probes data}, series = {Scientific reports}, volume = {12}, journal = {Scientific reports}, number = {1}, publisher = {Macmillan Publishers Limited, part of Springer Nature}, address = {London}, issn = {2045-2322}, doi = {10.1038/s41598-022-13302-1}, pages = {11}, year = {2022}, abstract = {In the last years, electron density profile functions characterized by a linear dependence on the scale height showed good results when approximating the topside ionosphere. The performance above 800 km, however, is not yet well investigated. This study investigates the capability of the semi-Epstein functions to represent electron density profiles from the peak height up to 20,000 km. Electron density observations recorded by the Van Allen Probes were used to resolve the scale height dependence in the plasmasphere. It was found that the linear dependence of the scale height in the topside ionosphere cannot be directly used to extrapolate profiles above 800 km. We find that the dependence of scale heights on altitude is quadratic in the plasmasphere. A statistical model of the scale heights is therefore proposed. After combining the topside ionosphere and plasmasphere by a unified model, we have obtained good estimations not only in the profile shapes, but also in the Total Electron Content magnitude and distributions when compared to actual measurements from 2013, 2014, 2016 and 2017. Our investigation shows that Van Allen Probes can be merged to radio-occultation data to properly represent the upper ionosphere and plasmasphere by means of a semi-Epstein function.}, language = {en} } @article{HaasShpritsAllisonetal.2022, author = {Haas, Bernhard and Shprits, Yuri and Allison, Hayley and Wutzig, Michael and Wang, Dedong}, title = {Which parameter controls ring current electron dynamics}, series = {Frontiers in astronomy and space sciences}, volume = {9}, journal = {Frontiers in astronomy and space sciences}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2296-987X}, doi = {10.3389/fspas.2022.911002}, pages = {11}, year = {2022}, abstract = {Predicting the electron population of Earth's ring current during geomagnetic storms still remains a challenging task. In this work, we investigate the sensitivity of 10 keV ring current electrons to different driving processes, parameterised by the Kp index, during several moderate and intense storms. Results are validated against measurements from the Van Allen Probes satellites. Perturbing the Kp index allows us to identify the most dominant processes for moderate and intense storms respectively. We find that during moderate storms (Kp < 6) the drift velocities mostly control the behaviour of low energy electrons, while loss from wave-particle interactions is the most critical parameter for quantifying the evolution of intense storms (Kp > 6). Perturbations of the Kp index used to drive the boundary conditions at GEO and set the plasmapause location only show a minimal effect on simulation results over a limited L range. It is further shown that the flux at L \& SIM; 3 is more sensitive to changes in the Kp index compared to higher L shells, making it a good proxy for validating the source-loss balance of a ring current model.}, language = {en} } @article{KuehnePaunovWeck2021, author = {K{\"u}hne, Franziska and Paunov, Tatjana and Weck, Florian}, title = {Recognizing obsessive-compulsive disorder}, series = {BMC psychiatry}, volume = {21}, journal = {BMC psychiatry}, publisher = {Springer Nature}, address = {London}, issn = {1471-244X}, doi = {10.1186/s12888-021-03458-x}, pages = {7}, year = {2021}, abstract = {Background Despite the prevalence of obsessive-compulsive disorder (OCD), its precise identification remains challenging. With the Zohar-Fineberg Obsessive-Compulsive Screen (ZF-OCS; 5 or 6 items), a brief instrument is widely available mainly in English. As there is a lack of empirical studies on the ZF-OCS, the aim of the present study was to translate the items into German and investigate the instrument in a nonclinical sample. Methods In two consecutive online surveys, n = 304 and n = 51 students participated. Besides the ZF-OCS, they answered established measures on OCD, depression, health anxiety, general anxiety and health-related well-being. Results Whereas internal consistency was low (α = .53-.72; ω = .55-.69), retest reliability (rt1,t2 = .89) at two weeks was high. As expected, we found high correlations with other OCD instruments (r > .61; convergent validity), and significantly weaker correlations with measures of depression (r = .39), health anxiety (r = .29), and health-related well-being (r = -.28, divergent validity). Nonetheless, the correlations with general anxiety were somewhere in between (r = .52). Conclusions Due to heterogeneous OCD subtypes, the ZF-OCS asks diverse questions which probably resulted in the present internal consistency. Nevertheless, the results on retest reliability and validity were promising. As for other OCD instruments, divergent validity regarding general anxiety seems problematic to establish. Even so, the ZF-OCS seems valuable for screening purposes, as it is short and easy to administer, and may facilitate initiating subsequent clinical assessment. Further studies should determine the instrument's diagnostic accuracy.}, language = {en} } @article{BornhorstSeyfried2021, author = {Bornhorst, Dorothee and Seyfried, Salim}, title = {Strong as a hippo's heart}, series = {Frontiers in cell and developmental biology}, volume = {9}, journal = {Frontiers in cell and developmental biology}, publisher = {Frontiers Media}, address = {Lausanne, Schweiz}, issn = {2296-634X}, doi = {10.3389/fcell.2021.731101}, pages = {1 -- 10}, year = {2021}, abstract = {The heart is comprised of multiple tissues that contribute to its physiological functions. During development, the growth of myocardium and endocardium is coupled and morphogenetic processes within these separate tissue layers are integrated. Here, we discuss the roles of mechanosensitive Hippo signaling in growth and morphogenesis of the zebrafish heart. Hippo signaling is involved in defining numbers of cardiac progenitor cells derived from the secondary heart field, in restricting the growth of the epicardium, and in guiding trabeculation and outflow tract formation. Recent work also shows that myocardial chamber dimensions serve as a blueprint for Hippo signaling-dependent growth of the endocardium. Evidently, Hippo pathway components act at the crossroads of various signaling pathways involved in embryonic zebrafish heart development. Elucidating how biomechanical Hippo signaling guides heart morphogenesis has direct implications for our understanding of cardiac physiology and pathophysiology.}, language = {en} } @article{SzangoliesRohwaederJeltsch2022, author = {Szangolies, Leonna and Rohw{\"a}der, Marie-Sophie and Jeltsch, Florian}, title = {Single large AND several small habitat patches}, series = {Basic and applied ecology : Journal of the Gesellschaft f{\"u}r {\"O}kologie}, volume = {65}, journal = {Basic and applied ecology : Journal of the Gesellschaft f{\"u}r {\"O}kologie}, publisher = {Elsevier}, address = {M{\"u}nchen}, issn = {1439-1791}, doi = {10.1016/j.baae.2022.09.004}, pages = {16 -- 27}, year = {2022}, abstract = {The debate whether single large or several small (SLOSS) patches benefit biodiversity has existed for decades, but recent literature provides increasing evidence for the importance of small habitats. Possible beneficial mechanisms include reduced presence of preda-tors and competitors in small habitat areas or specific functions such as stepping stones for dispersal. Given the increasing amount of studies highlighting individual behavioral differences that may influence these functions, we hypothesize that the advantage of small versus large habitat patches not only depends on patch functionality but also on the presence of animal personalities (i.e., risk-tolerant vs. risk-averse). Using an individual-based, spatially-explicit community model, we analyzed the diversity of mammal communities in landscapes consisting of a few large habitat islands interspersed with different amounts and sizes of small habitat patches. Within these heterogeneous environments, individuals compete for resources and form home-ranges, with only risk-tolerant individuals using habitat edges. Results show that when risk-tolerant individuals exist, small patches increase species diversity. A strong peak occurs at approximately 20\% habitat cover in small patches when those small habitats are only used for foraging but not for breeding and home-range core position. Additional usage as stepping stones for juvenile dispersal further increases species persistence. Over-all, our results reveal that a combination of a few large and several small habitat patches promotes biodiversity by enhancing land-scape heterogeneity. Here, heterogeneity is created by pronounced differences in habitat functionality, increasing edge density, and variability in habitat use by different behavioral types. The finding that a combination of single large AND several small (SLASS) patches is needed for effective biodiversity preservation has implications for advancing landscape conservation. Particularly in struc-turally poor agricultural areas, modern technology enables precise management with the opportunity to create small foraging habitats by excluding less profitable agricultural land from cultivation.}, language = {en} } @article{KuekenTrevesNikoloski2023, author = {K{\"u}ken, Anika and Treves, Haim and Nikoloski, Zoran}, title = {A simulation-free constrained regression approach for flux estimation in isotopically nonstationary metabolic flux analysis with applications in microalgae}, series = {Frontiers in plant science : FPLS}, volume = {14}, journal = {Frontiers in plant science : FPLS}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {1664-462X}, doi = {10.3389/fpls.2023.1140829}, pages = {12}, year = {2023}, abstract = {Introduction Flux phenotypes from different organisms and growth conditions allow better understanding of differential metabolic networks functions. Fluxes of metabolic reactions represent the integrated outcome of transcription, translation, and post-translational modifications, and directly affect growth and fitness. However, fluxes of intracellular metabolic reactions cannot be directly measured, but are estimated via metabolic flux analysis (MFA) that integrates data on isotope labeling patterns of metabolites with metabolic models. While the application of metabolomics technologies in photosynthetic organisms have resulted in unprecedented data from 13CO2-labeling experiments, the bottleneck in flux estimation remains the application of isotopically nonstationary MFA (INST-MFA). INST-MFA entails fitting a (large) system of coupled ordinary differential equations, with metabolite pools and reaction fluxes as parameters. Here, we focus on the Calvin-Benson cycle (CBC) as a key pathway for carbon fixation in photosynthesizing organisms and ask if approaches other than classical INST-MFA can provide reliable estimation of fluxes for reactions comprising this pathway. Methods First, we show that flux estimation with the labeling patterns of all CBC intermediates can be formulated as a single constrained regression problem, avoiding the need for repeated simulation of time-resolved labeling patterns. Results We then compare the flux estimates of the simulation-free constrained regression approach with those obtained from the classical INST-MFA based on labeling patterns of metabolites from the microalgae Chlamydomonas reinhardtii, Chlorella sorokiniana and Chlorella ohadii under different growth conditions. Discussion Our findings indicate that, in data-rich scenarios, simulation-free regression-based approaches provide a suitable alternative for flux estimation from classical INST-MFA since we observe a high qualitative agreement (rs=0.89) to predictions obtained from INCA, a state-of-the-art tool for INST-MFA.}, language = {en} } @article{PuchkovMuellerLehmannetal.2023, author = {Puchkov, Dmytro and M{\"u}ller, Paul Markus and Lehmann, Martin and Matth{\"a}us, Claudia}, title = {Analyzing the cellular plasma membrane by fast and efficient correlative STED and platinum replica EM}, series = {Frontiers in cell and developmental biology}, volume = {11}, journal = {Frontiers in cell and developmental biology}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2296-634X}, doi = {10.3389/fcell.2023.1305680}, pages = {15}, year = {2023}, abstract = {The plasma membrane of mammalian cells links transmembrane receptors, various structural components, and membrane-binding proteins to subcellular processes, allowing inter- and intracellular communication. Therefore, membrane-binding proteins, together with structural components such as actin filaments, modulate the cell membrane in their flexibility, stiffness, and curvature. Investigating membrane components and curvature in cells remains challenging due to the diffraction limit in light microscopy. Preparation of 5-15-nm-thin plasma membrane sheets and subsequent inspection by metal replica transmission electron microscopy (TEM) reveal detailed information about the cellular membrane topology, including the structure and curvature. However, electron microscopy cannot identify proteins associated with specific plasma membrane domains. Here, we describe a novel adaptation of correlative super-resolution light microscopy and platinum replica TEM (CLEM-PREM), allowing the analysis of plasma membrane sheets with respect to their structural details, curvature, and associated protein composition. We suggest a number of shortcuts and troubleshooting solutions to contemporary PREM protocols. Thus, implementation of super-resolution stimulated emission depletion (STED) microscopy offers significant reduction in sample preparation time and reduced technical challenges for imaging and analysis. Additionally, highly technical challenges associated with replica preparation and transfer on a TEM grid can be overcome by scanning electron microscopy (SEM) imaging. The combination of STED microscopy and platinum replica SEM or TEM provides the highest spatial resolution of plasma membrane proteins and their underlying membrane and is, therefore, a suitable method to study cellular events like endocytosis, membrane trafficking, or membrane tension adaptations.}, language = {en} } @article{WojcikCeulemansGaedke2021, author = {Wojcik, Laurie Anne Myriam and Ceulemans, Ruben and Gaedke, Ursula}, title = {Functional diversity buffers the effects of a pulse perturbation on the dynamics of tritrophic food webs}, series = {Ecology and Evolution}, volume = {11}, journal = {Ecology and Evolution}, number = {22}, publisher = {John Wiley \& Sons, Inc.}, address = {Hoboken (New Jersey)}, issn = {2045-7758}, doi = {10.1002/ece3.8214}, pages = {15639 -- 15663}, year = {2021}, abstract = {Biodiversity decline causes a loss of functional diversity, which threatens ecosystems through a dangerous feedback loop: This loss may hamper ecosystems' ability to buffer environmental changes, leading to further biodiversity losses. In this context, the increasing frequency of human-induced excessive loading of nutrients causes major problems in aquatic systems. Previous studies investigating how functional diversity influences the response of food webs to disturbances have mainly considered systems with at most two functionally diverse trophic levels. We investigated the effects of functional diversity on the robustness, that is, resistance, resilience, and elasticity, using a tritrophic—and thus more realistic—plankton food web model. We compared a non-adaptive food chain with no diversity within the individual trophic levels to a more diverse food web with three adaptive trophic levels. The species fitness differences were balanced through trade-offs between defense/growth rate for prey and selectivity/half-saturation constant for predators. We showed that the resistance, resilience, and elasticity of tritrophic food webs decreased with larger perturbation sizes and depended on the state of the system when the perturbation occurred. Importantly, we found that a more diverse food web was generally more resistant and resilient but its elasticity was context-dependent. Particularly, functional diversity reduced the probability of a regime shift toward a non-desirable alternative state. The basal-intermediate interaction consistently determined the robustness against a nutrient pulse despite the complex influence of the shape and type of the dynamical attractors. This relationship was strongly influenced by the diversity present and the third trophic level. Overall, using a food web model of realistic complexity, this study confirms the destructive potential of the positive feedback loop between biodiversity loss and robustness, by uncovering mechanisms leading to a decrease in resistance, resilience, and potentially elasticity as functional diversity declines.}, language = {en} } @misc{Krause2023, author = {Krause, Werner}, title = {Die Macht der Sonntagsfrage}, series = {Verfassungsblog : on matters constitutional}, journal = {Verfassungsblog : on matters constitutional}, publisher = {Max Steinbeis Verfassungsblog gGmbH}, address = {Berlin}, issn = {2366-7044}, doi = {10.17176/20231222-111226-0}, pages = {7}, year = {2023}, abstract = {F{\"u}r das Jahr 2024 sind entscheidende Wahlen geplant - unter ihnen die US-Pr{\"a}sidentschaftswahl und die Wahlen zum Europ{\"a}ischen Parlament. In Deutschland werden in Brandenburg, Sachsen und Th{\"u}ringen die Landtage gew{\"a}hlt. Wahlumfragen, insbesondere die Sonntagsfrage, sind zu einem integralen Bestandteil von Wahlk{\"a}mpfen geworden; gleichzeitig steht auch deren Zuverl{\"a}ssigkeit im Zentrum medialer Aufmerksamkeit. Eine Debatte {\"u}ber die Kommunikation und Darstellung von Meinungsumfragen ist in Deutschland dringend notwendig. Eine bindende Selbstverpflichtung der Umfrageinstitute und Medienh{\"a}user w{\"a}re eine vielversprechende L{\"o}sung.}, language = {de} } @inproceedings{Schladebach2022, author = {Schladebach, Marcus}, title = {Satelliten-Megakonstellationen im Weltraumrecht}, series = {Tagungsband zur Sommerkonferenz 2022 : Telemedicus - Recht der Informationsgesellschaft}, volume = {6}, booktitle = {Tagungsband zur Sommerkonferenz 2022 : Telemedicus - Recht der Informationsgesellschaft}, editor = {Gr{\"a}fe, Hans-Christian}, publisher = {Fachmedien Recht und Wirtschaft, dfv Mediengruppe}, address = {Frankfurt am Main}, isbn = {978-3-8005-1857-9}, pages = {68 -- 75}, year = {2022}, language = {de} }