@article{Goychuk2018, author = {Goychuk, Igor}, title = {Sensing magnetic fields with magnetosensitive ion channels}, series = {Sensors}, volume = {18}, journal = {Sensors}, number = {3}, publisher = {MDPI}, address = {Basel}, issn = {1424-8220}, doi = {10.3390/s18030728}, pages = {19}, year = {2018}, abstract = {Magnetic nanoparticles are met across many biological species ranging from magnetosensitive bacteria, fishes, bees, bats, rats, birds, to humans. They can be both of biogenetic origin and due to environmental contamination, being either in paramagnetic or ferromagnetic state. The energy of such naturally occurring single-domain magnetic nanoparticles can reach up to 10-20 room k(B)T in the magnetic field of the Earth, which naturally led to supposition that they can serve as sensory elements in various animals. This work explores within a stochastic modeling framework a fascinating hypothesis of magnetosensitive ion channels with magnetic nanoparticles serving as sensory elements, especially, how realistic it is given a highly dissipative viscoelastic interior of living cells and typical sizes of nanoparticles possibly involved.}, language = {en} } @misc{Kleuser2018, author = {Kleuser, Burkhard}, title = {Divergent role of sphingosine 1-phosphate in liver health and disease}, series = {International journal of molecular sciences}, volume = {19}, journal = {International journal of molecular sciences}, number = {3}, publisher = {MDPI}, address = {Basel}, issn = {1422-0067}, doi = {10.3390/ijms19030722}, pages = {18}, year = {2018}, abstract = {Two decades ago, sphingosine 1-phosphate (S1P) was discovered as a novel bioactive molecule that regulates a variety of cellular functions. The plethora of S1P-mediated effects is due to the fact that the sphingolipid not only modulates intracellular functions but also acts as a ligand of G protein-coupled receptors after secretion into the extracellular environment. In the plasma, S1P is found in high concentrations, modulating immune cell trafficking and vascular endothelial integrity. The liver is engaged in modulating the plasma S1P content, as it produces apolipoprotein M, which is a chaperone for the S1P transport. Moreover, the liver plays a substantial role in glucose and lipid homeostasis. A dysfunction of glucose and lipid metabolism is connected with the development of liver diseases such as hepatic insulin resistance, non-alcoholic fatty liver disease, or liver fibrosis. Recent studies indicate that S1P is involved in liver pathophysiology and contributes to the development of liver diseases. In this review, the current state of knowledge about S1P and its signaling in the liver is summarized with a specific focus on the dysregulation of S1P signaling in obesity-mediated liver diseases. Thus, the modulation of S1P signaling can be considered as a potential therapeutic target for the treatment of hepatic diseases.}, language = {en} } @article{FuchsGrosseStraussetal.2018, author = {Fuchs, Matthias and Grosse, Guido and Strauss, Jens and G{\"u}nther, Frank and Grigoriev, Mikhail N. and Maximov, Georgy M. and Hugelius, Gustaf}, title = {Carbon and nitrogen pools in thermokarst-affected permafrost landscapes in Arctic Siberia}, series = {Biogeosciences}, volume = {15}, journal = {Biogeosciences}, number = {3}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1726-4170}, doi = {10.5194/bg-15-953-2018}, pages = {953 -- 971}, year = {2018}, abstract = {Ice-rich yedoma-dominated landscapes store considerable amounts of organic carbon (C) and nitrogen (N) and are vulnerable to degradation under climate warming. We investigate the C and N pools in two thermokarst-affected yedoma landscapes - on Sobo-Sise Island and on Bykovsky Peninsula in the north of eastern Siberia. Soil cores up to 3m depth were collected along geomorphic gradients and analysed for organic C and N contents. A high vertical sampling density in the profiles allowed the calculation of C and N stocks for short soil column intervals and enhanced understanding of within-core parameter variability. Profile-level C and N stocks were scaled to the landscape level based on landform classifications from 5 m resolution, multispectral RapidEye satellite imagery. Mean landscape C and N storage in the first metre of soil for Sobo-Sise Island is estimated to be 20.2 kg C m(-2) and 1.8 kg N m(-2) and for Bykovsky Peninsula 25.9 kg C m(-2) and 2.2 kg N m(-2). Radiocarbon dating demonstrates the Holocene age of thermokarst basin deposits but also suggests the presence of thick Holoceneage cover layers which can reach up to 2 m on top of intact yedoma landforms. Reconstructed sedimentation rates of 0.10-0.57 mm yr(-1) suggest sustained mineral soil accumulation across all investigated landforms. Both yedoma and thermokarst landforms are characterized by limited accumulation of organic soil layers (peat). We further estimate that an active layer deepening of about 100 cm will increase organic C availability in a seasonally thawed state in the two study areas by similar to 5.8 Tg (13.2 kg C m(-2)). Our study demonstrates the importance of increasing the number of C and N storage inventories in ice-rich yedoma and thermokarst environments in order to account for high variability of permafrost and thermokarst environments in pan-permafrost soil C and N pool estimates.}, language = {en} } @article{SauermannHoehle2018, author = {Sauermann, Antje and H{\"o}hle, Barbara}, title = {Word order in German child language and child-directed speech}, series = {Glossa: A Journal of General Linguistics}, volume = {3}, journal = {Glossa: A Journal of General Linguistics}, number = {1}, publisher = {Ubiquity Press LTD}, address = {London}, issn = {2397-1835}, doi = {10.5334/gjgl.281}, pages = {32}, year = {2018}, abstract = {We report two corpus analyses to examine the impact of animacy, definiteness, givenness and type of referring expression on the ordering of double objects in the spontaneous speech of German-speaking two- to four-year-old children and the child-directed speech of their mothers. The first corpus analysis revealed that definiteness, givenness and type of referring expression influenced word order variation in child language and child-directed speech when the type of referring expression distinguished between pronouns and lexical noun phrases. These results correspond to previous child language studies in English (e.g., de Marneffe et al. 2012). Extending the scope of previous studies, our second corpus analysis examined the role of different pronoun types on word order. It revealed that word order in child language and child-directed speech was predictable from the types of pronouns used. Different types of pronouns were associated with different sentence positions but also showed a strong correlation to givenness and definiteness. Yet, the distinction between pronoun types diminished the effects of givenness so that givenness had an independent impact on word order only in child-directed speech but not in child language. Our results support a multi-factorial approach to word order in German. Moreover, they underline the strong impact of the type of referring expression on word order and suggest that it plays a crucial role in the acquisition of the factors influencing word order variation.}, language = {en} } @article{TaffarelloSrinivasanSamprognaMohoretal.2018, author = {Taffarello, Denise and Srinivasan, Raghavan and Samprogna Mohor, Guilherme and Bittencourt Guimaraes, Joao Luis and Calijuri, Maria do Carmo and Mendiondo, Eduardo Mario}, title = {Modeling freshwater quality scenarios with ecosystem-based adaptation in the headwaters of the Cantareira system, Brazil}, series = {Hydrology and earth system sciences : HESS}, volume = {22}, journal = {Hydrology and earth system sciences : HESS}, number = {9}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1027-5606}, doi = {10.5194/hess-22-4699-2018}, pages = {4699 -- 4723}, year = {2018}, abstract = {Although hydrologic models provide hypothesis testing of complex dynamics occurring at catchments, fresh-water quality modeling is still incipient at many subtropical headwaters. In Brazil, a few modeling studies assess freshwater nutrients, limiting policies on hydrologic ecosystem services. This paper aims to compare freshwater quality scenarios under different land-use and land-cover (LULC) change, one of them related to ecosystem-based adaptation (EbA), in Brazilian headwaters. Using the spatially semi-distributed Soil and Water Assessment Tool (SWAT) model, nitrate, total phosphorous (TP) and sediment were modeled in catchments ranging from 7.2 to 1037 km(2). These head-waters were eligible areas of the Brazilian payment for ecosystem services (PES) projects in the Cantareira water supply system, which had supplied water to 9 million people in the Sao Paulo metropolitan region (SPMR). We considered SWAT modeling of three LULC scenarios: (i) recent past scenario (S1), with historical LULC in 1990; (ii) current land-use scenario (S2), with LULC for the period 2010-2015 with field validation; and (iii) future land-use scenario with PES (S2 + EbA). This latter scenario proposed forest cover restoration through EbA following the river basin plan by 2035. These three LULC scenarios were tested with a selected record of rainfall and evapotranspiration observed in 2006-2014, with the occurrence of extreme droughts. To assess hydrologic services, we proposed the hydrologic service index (HSI), as a new composite metric comparing water pollution levels (WPL) for reference catchments, related to the grey water footprint (greyWF) and water yield. On the one hand, water quality simulations allowed for the regionalization of greyWF at spatial scales under LULC scenarios. According to the critical threshold, HSI identified areas as less or more sustainable catchments. On the other hand, conservation practices simulated through the S2 + EbA scenario envisaged not only additional and viable best management practices (BMP), but also preventive decision-making at the headwaters of water supply systems.}, language = {en} } @article{HeidlerSalzwedelJoebgesetal.2018, author = {Heidler, Maria-Dorothea and Salzwedel, Annett and J{\"o}bges, Michael and L{\"u}ck, Olaf and Dohle, Christian and Seifert, Michael and von Helden, Andrea and Hollweg, Wibke and V{\"o}ller, Heinz}, title = {Decannulation of tracheotomized patients after long-term mechanical ventilation - results of a prospective multicentric study in German neurological early rehabilitation hospitals}, series = {BMC Anesthesiology}, volume = {18}, journal = {BMC Anesthesiology}, publisher = {BMC}, address = {London}, issn = {1471-2253}, doi = {10.1186/s12871-018-0527-3}, pages = {7}, year = {2018}, abstract = {Background: In the course of neurological early rehabilitation, decannulation is attempted in tracheotomized patients after weaning due to its considerable prognostic significance. We aimed to identify predictors of a successful tracheostomy decannulation. Methods: From 09/2014 to 03/2016, 831 tracheotomized and weaned patients (65.4 +/- 12.9 years, 68\% male) were included consecutively in a prospective multicentric observation study. At admission, sociodemographic and clinical data (e.g. relevant neurological and internistic diseases, duration of mechanical ventilation, tracheotomy technique, and nutrition) as well as functional assessments (Coma Recovery Scale-Revised (CRS-R), Early Rehabilitation Barthel Index, Bogenhausener Dysphagia Score) were collected. Complications and the success of the decannulation procedure were documented at discharge. Results: Four hundred seventy patients (57\%) were decannulated. The probability of decannulation was significantly negatively associated with increasing age (OR 0.68 per SD = 12.9 years, p < 0.001), prolonged duration of mechanical ventilation (OR 0.57 per 33.2 days, p < 0.001) and complications. An oral diet (OR 3.80; p < 0.001) and a higher alertness at admission (OR 3.07 per 7.18 CRS-R points; p < 0.001) were positively associated. Conclusions: This study identified practically measurable predictors of decannulation, which in the future can be used for a decannulation prognosis and supply optimization at admission in the neurological early rehabilitation clinic.}, language = {en} } @article{SenczukHavensteinMilanaetal.2018, author = {Senczuk, Gabriele and Havenstein, Katja and Milana, Valentina and Ripa, Chiara and De Simone, Emanuela and Tiedemann, Ralph and Castiglia, Riccardo}, title = {Spotlight on islands}, series = {Scientific reports}, volume = {8}, journal = {Scientific reports}, publisher = {Nature Publ. Group}, address = {London}, issn = {2045-2322}, doi = {10.1038/s41598-018-33326-w}, pages = {12}, year = {2018}, abstract = {Groups of proximate continental islands may conceal more tangled phylogeographic patterns than oceanic archipelagos as a consequence of repeated sea level changes, which allow populations to experience gene flow during periods of low sea level stands and isolation by vicariant mechanisms during periods of high sea level stands. Here, we describe for the first time an ancient and diverging lineage of the Italian wall lizard Podarcis siculus from the western Pontine Islands. We used nuclear and mitochondrial DNA sequences of 156 individuals with the aim of unraveling their phylogenetic position, while microsatellite loci were used to test several a priori insular biogeographic models of migration with empirical data. Our results suggest that the western Pontine populations colonized the islands early during their Pliocene volcanic formation, while populations from the eastern Pontine Islands seem to have been introduced recently. The inter-island genetic makeup indicates an important role of historical migration, probably due to glacial land bridges connecting islands followed by a recent vicariant mechanism of isolation. Moreover, the most supported migration model predicted higher gene flow among islands which are geographically arranged in parallel. Considering the threatened status of small insular endemic populations, we suggest this new evolutionarily independent unit be given priority in conservation efforts.}, language = {en} } @misc{MotaLeckeltGeukesetal.2018, author = {Mota, Simon and Leckelt, Marius and Geukes, Katharina and Nestler, Steffen and Humberg, Sarah and Schr{\"o}der-Abe, Michela and Schmukle, Stefan C. and Back, Mitja D.}, title = {A comprehensive examination of narcissists' self-perceived and actual socioemotional cognition ability}, series = {Collabra: Psychology}, volume = {5}, journal = {Collabra: Psychology}, number = {1}, publisher = {University of California Press}, address = {Oakland}, issn = {2474-7394}, doi = {10.1525/collabra.174}, pages = {25}, year = {2018}, abstract = {Narcissists are assumed to lack the motivation and ability to share and understand the mental states of others. Prior empirical research, however, has yielded inconclusive findings and has differed with respect to the specific aspects of narcissism and socioemotional cognition that have been examined. Here, we propose a differentiated facet approach that can be applied across research traditions and that distinguishes between facets of narcissism (agentic vs. antagonistic) on the one hand, and facets of socioemotional cognition ability (SECA; self-perceived vs. actual) on the other. Using five nonclinical samples in two studies (total N = 602), we investigated the effect of facets of grandiose narcissism on aspects of socioemotional cognition across measures of affective and cognitive empathy, Theory of Mind, and emotional intelligence, while also controlling for general reasoning ability. Across both studies, agentic facets of narcissism were found to be positively related to perceived SECA, whereas antagonistic facets of narcissism were found to be negatively related to perceived SECA. However, both narcissism facets were negatively related to actual SECA. Exploratory condition-based regression analyses further showed that agentic narcissists had a higher directed discrepancy between perceived and actual SECA: They self-enhanced their socio-emotional capacities. Implications of these results for the multifaceted theoretical understanding of the narcissism-SECA link are discussed.}, language = {en} } @article{VidalGarciaBandaraKeogh2018, author = {Vidal-Garcia, Marta and Bandara, Lashi and Keogh, J. Scott}, title = {ShapeRotator}, series = {Ecology and evolution}, volume = {8}, journal = {Ecology and evolution}, number = {9}, publisher = {Wiley}, address = {Hoboken}, issn = {2045-7758}, doi = {10.1002/ece3.4018}, pages = {4669 -- 4675}, year = {2018}, abstract = {The quantification of complex morphological patterns typically involves comprehensive shape and size analyses, usually obtained by gathering morphological data from all the structures that capture the phenotypic diversity of an organism or object. Articulated structures are a critical component of overall phenotypic diversity, but data gathered from these structures are difficult to incorporate into modern analyses because of the complexities associated with jointly quantifying 3D shape in multiple structures. While there are existing methods for analyzing shape variation in articulated structures in two-dimensional (2D) space, these methods do not work in 3D, a rapidly growing area of capability and research. Here, we describe a simple geometric rigid rotation approach that removes the effect of random translation and rotation, enabling the morphological analysis of 3D articulated structures. Our method is based on Cartesian coordinates in 3D space, so it can be applied to any morphometric problem that also uses 3D coordinates (e.g., spherical harmonics). We demonstrate the method by applying it to a landmark-based dataset for analyzing shape variation using geometric morphometrics. We have developed an R tool (ShapeRotator) so that the method can be easily implemented in the commonly used R package geomorph and MorphoJ software. This method will be a valuable tool for 3D morphological analyses in articulated structures by allowing an exhaustive examination of shape and size diversity.}, language = {en} } @article{TutuSteinbergerSobolevetal.2018, author = {Tutu, Anthony Osei and Steinberger, Bernhard and Sobolev, Stephan Vladimir and Rogozhina, Irina and Popov, Anton A.}, title = {Effects of upper mantle heterogeneities on the lithospheric stress field and dynamic topography}, series = {Solid earth}, volume = {9}, journal = {Solid earth}, number = {3}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1869-9510}, doi = {10.5194/se-9-649-2018}, pages = {649 -- 668}, year = {2018}, abstract = {The orientation and tectonic regime of the observed crustal/lithospheric stress field contribute to our knowledge of different deformation processes occurring within the Earth's crust and lithosphere. In this study, we analyze the influence of the thermal and density structure of the upper mantle on the lithospheric stress field and topography. We use a 3-D lithosphere-asthenosphere numerical model with power-law rheology, coupled to a spectral mantle flow code at 300 km depth. Our results are validated against the World Stress Map 2016 (WSM2016) and the observation-based residual topography. We derive the upper mantle thermal structure from either a heat flow model combined with a seafloor age model (TM1) or a global S-wave velocity model (TM2). We show that lateral density heterogeneities in the upper 300 km have a limited influence on the modeled horizontal stress field as opposed to the resulting dynamic topography that appears more sensitive to such heterogeneities. The modeled stress field directions, using only the mantle heterogeneities below 300 km, are not perturbed much when the effects of lithosphere and crust above 300 km are added. In contrast, modeled stress magnitudes and dynamic topography are to a greater extent controlled by the upper mantle density structure. After correction for the chemical depletion of continents, the TM2 model leads to a much better fit with the observed residual topography giving a good correlation of 0.51 in continents, but this correction leads to no significant improvement of the fit between the WSM2016 and the resulting lithosphere stresses. In continental regions with abundant heat flow data, TM1 results in relatively small angular misfits. For example, in western Europe the misfit between the modeled and observation-based stress is 18.3°. Our findings emphasize that the relative contributions coming from shallow and deep mantle dynamic forces are quite different for the lithospheric stress field and dynamic topography.}, language = {en} } @article{PitzenAskarzadaGraefetal.2018, author = {Pitzen, Valentin and Askarzada, Sophie and Gr{\"a}f, Ralph and Meyer, Irene}, title = {CDK5RAP2 Is an Essential Scaffolding Protein of the Corona of the Dictyostelium Centrosome}, series = {Cells}, volume = {7}, journal = {Cells}, number = {4}, publisher = {MDPI}, address = {Basel}, issn = {2073-4409}, doi = {10.3390/cells7040032}, pages = {17}, year = {2018}, abstract = {Dictyostelium centrosomes consist of a nucleus-associated cylindrical, three-layered core structure surrounded by a corona consisting of microtubule-nucleation complexes embedded in a scaffold of large coiled-coil proteins. One of them is the conserved CDK5RAP2 protein. Here we focus on the role of Dictyostelium CDK5RAP2 for maintenance of centrosome integrity, its interaction partners and its dynamic behavior during interphase and mitosis. GFP-CDK5RAP2 is present at the centrosome during the entire cell cycle except from a short period during prophase, correlating with the normal dissociation of the corona at this stage. RNAi depletion of CDK5RAP2 results in complete disorganization of centrosomes and microtubules suggesting that CDK5RAP2 is required for organization of the corona and its association to the core structure. This is in line with the observation that overexpressed GFP-CDK5RAP2 elicited supernumerary cytosolic MTOCs. The phenotype of CDK5RAP2 depletion was very reminiscent of that observed upon depletion of CP148, another scaffolding protein of the corona. BioID interaction assays revealed an interaction of CDK5RAP2 not only with the corona markers CP148, gamma-tubulin, and CP248, but also with the core components Cep192, CP75, and CP91. Furthermore, protein localization studies in both depletion strains revealed that CP148 and CDK5RAP2 cooperate in corona organization.}, language = {en} } @misc{Graef2018, author = {Gr{\"a}f, Ralph}, title = {Comparative Biology of Centrosomal Structures in Eukaryotes}, series = {Cells}, volume = {7}, journal = {Cells}, number = {11}, publisher = {MDPI}, address = {Basel}, issn = {2073-4409}, doi = {10.3390/cells7110202}, pages = {9}, year = {2018}, abstract = {The centrosome is not only the largest and most sophisticated protein complex within a eukaryotic cell, in the light of evolution, it is also one of its most ancient organelles. This special issue of "Cells" features representatives of three main, structurally divergent centrosome types, i.e., centriole-containing centrosomes, yeast spindle pole bodies (SPBs), and amoebozoan nucleus-associated bodies (NABs). Here, I discuss their evolution and their key-functions in microtubule organization, mitosis, and cytokinesis. Furthermore, I provide a brief history of centrosome research and highlight recently emerged topics, such as the role of centrioles in ciliogenesis, the relationship of centrosomes and centriolar satellites, the integration of centrosomal structures into the nuclear envelope and the involvement of centrosomal components in non-centrosomal microtubule organization.}, language = {en} } @article{KrasotkinaGoetzHoehleetal.2018, author = {Krasotkina, Anna and G{\"o}tz, Antonia and H{\"o}hle, Barbara and Schwarzer, Gudrun}, title = {Perceptual narrowing in speech and face recognition}, series = {Frontiers in psychology}, volume = {9}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2018.01711}, pages = {5}, year = {2018}, abstract = {During the first year of life, infants undergo perceptual narrowing in the domains of speech and face perception. This is typically characterized by improvements in infants' abilities in discriminating among stimuli of familiar types, such as native speech tones and same-race faces. Simultaneously, infants begin to decline in their ability to discriminate among stimuli of types with which they have little experience, such as nonnative tones and other-race faces. The similarity in time-frames during which perceptual narrowing seems to occur in the domains of speech and face perception has led some researchers to hypothesize that the perceptual narrowing in these domains could be driven by shared domain-general processes. To explore this hypothesis, we tested 53 Caucasian 9-month-old infants from monolingual German households on their ability to discriminate among non-native Cantonese speech tones, as well among same-race German faces and other-race Chinese faces. We tested the infants using an infant-controlled habituation-dishabituation paradigm, with infants' preferences for looking at novel stimuli versus the habituated stimuli (dishabituation scores) acting as indicators of discrimination ability. As expected for their age, infants were able to discriminate between same-race faces, but not between other-race faces or non-native speech tones. Most interestingly, we found that infants' dishabituation scores for the non-native speech tones and other-race faces showed significant positive correlations, while the dishabituation scores for non-native speech tones and same-race faces did not. These results therefore support the hypothesis that shared domain-general mechanisms may drive perceptual narrowing in the domains of speech and face perception.}, language = {en} } @article{vanVelzenGaedke2018, author = {van Velzen, Ellen and Gaedke, Ursula}, title = {Reversed predator-prey cycles are driven by the amplitude of prey oscillations}, series = {Ecology and evolution}, volume = {8}, journal = {Ecology and evolution}, number = {12}, publisher = {Wiley}, address = {Hoboken}, issn = {2045-7758}, doi = {10.1002/ece3.4184}, pages = {6317 -- 6329}, year = {2018}, abstract = {Ecoevolutionary feedbacks in predator-prey systems have been shown to qualitatively alter predator-prey dynamics. As a striking example, defense-offense coevolution can reverse predator-prey cycles, so predator peaks precede prey peaks rather than vice versa. However, this has only rarely been shown in either model studies or empirical systems. Here, we investigate whether this rarity is a fundamental feature of reversed cycles by exploring under which conditions they should be found. For this, we first identify potential conditions and parameter ranges most likely to result in reversed cycles by developing a new measure, the effective prey biomass, which combines prey biomass with prey and predator traits, and represents the prey biomass as perceived by the predator. We show that predator dynamics always follow the dynamics of the effective prey biomass with a classic 1/4-phase lag. From this key insight, it follows that in reversed cycles (i.e., -lag), the dynamics of the actual and the effective prey biomass must be in antiphase with each other, that is, the effective prey biomass must be highest when actual prey biomass is lowest, and vice versa. Based on this, we predict that reversed cycles should be found mainly when oscillations in actual prey biomass are small and thus have limited impact on the dynamics of the effective prey biomass, which are mainly driven by trait changes. We then confirm this prediction using numerical simulations of a coevolutionary predator-prey system, varying the amplitude of the oscillations in prey biomass: Reversed cycles are consistently associated with regions of parameter space leading to small-amplitude prey oscillations, offering a specific and highly testable prediction for conditions under which reversed cycles should occur in natural systems.}, language = {en} } @article{SchoenemannLaschewskyRosenhahn2018, author = {Sch{\"o}nemann, Eric and Laschewsky, Andre and Rosenhahn, Axel}, title = {Exploring the long-term hydrolytic behavior of zwitterionic polymethacrylates and polymethacrylamides}, series = {Polymers}, volume = {10}, journal = {Polymers}, number = {6}, publisher = {MDPI}, address = {Basel}, issn = {2073-4360}, doi = {10.3390/polym10060639}, pages = {23}, year = {2018}, abstract = {The hydrolytic stability of polymers to be used for coatings in aqueous environments, for example, to confer anti-fouling properties, is crucial. However, long-term exposure studies on such polymers are virtually missing. In this context, we synthesized a set of nine polymers that are typically used for low-fouling coatings, comprising the well-established poly(oligoethylene glycol methylether methacrylate), poly(3-(N-2-methacryloylethyl-N,N-dimethyl) ammoniopropanesulfonate) (sulfobetaine methacrylate), and poly(3-(N-3-methacryamidopropyl-N,N-dimethyl)ammoniopropanesulfonate) (sulfobetaine methacrylamide) as well as a series of hitherto rarely studied polysulfabetaines, which had been suggested to be particularly hydrolysis-stable. Hydrolysis resistance upon extended storage in aqueous solution is followed by H-1 NMR at ambient temperature in various pH regimes. Whereas the monomers suffered slow (in PBS) to very fast hydrolysis (in 1 M NaOH), the polymers, including the polymethacrylates, proved to be highly stable. No degradation of the carboxyl ester or amide was observed after one year in PBS, 1 M HCl, or in sodium carbonate buffer of pH 10. This demonstrates their basic suitability for anti-fouling applications. Poly(sulfobetaine methacrylamide) proved even to be stable for one year in 1 M NaOH without any signs of degradation. The stability is ascribed to a steric shielding effect. The hemisulfate group in the polysulfabetaines, however, was found to be partially labile.}, language = {en} } @article{EhrlichGaedke2018, author = {Ehrlich, Elias and Gaedke, Ursula}, title = {Not attackable or not crackable}, series = {Ecology and evolution}, volume = {8}, journal = {Ecology and evolution}, number = {13}, publisher = {Wiley}, address = {Hoboken}, issn = {2045-7758}, doi = {10.1002/ece3.4145}, pages = {6625 -- 6637}, year = {2018}, abstract = {It is well-known that prey species often face trade-offs between defense against predation and competitiveness, enabling predator-mediated coexistence. However, we lack an understanding of how the large variety of different defense traits with different competition costs affects coexistence and population dynamics. Our study focusses on two general defense mechanisms, that is, pre-attack (e.g., camouflage) and post-attack defenses (e.g., weaponry) that act at different phases of the predator—prey interaction. We consider a food web model with one predator, two prey types and one resource. One prey type is undefended, while the other one is pre- or post-attack defended paying costs either by a higher half-saturation constant for resource uptake or a lower maximum growth rate. We show that post-attack defenses promote prey coexistence and stabilize the population dynamics more strongly than pre-attack defenses by interfering with the predator's functional response: Because the predator spends time handling "noncrackable" prey, the undefended prey is indirectly facilitated. A high half-saturation constant as defense costs promotes coexistence more and stabilizes the dynamics less than a low maximum growth rate. The former imposes high costs at low resource concentrations but allows for temporally high growth rates at predator-induced resource peaks preventing the extinction of the defended prey. We evaluate the effects of the different defense mechanisms and costs on coexistence under different enrichment levels in order to vary the importance of bottom-up and top-down control of the prey community.}, language = {en} } @article{PoltrockChenKwoketal.2018, author = {Poltrock, Silvana and Chen, Hui and Kwok, Celia and Cheung, Hintat and Nazzi, Thierry}, title = {Adult learning of novel words in a non-native language}, series = {Frontiers in psychology}, volume = {9}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2018.01211}, pages = {15}, year = {2018}, language = {en} } @misc{KewenigZhouFischer2018, author = {Kewenig, Viktor and Zhou, Yuefang and Fischer, Martin H.}, title = {Commentary: Robots as intentional agents}, series = {Frontiers in psychology}, volume = {9}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2018.01131}, pages = {2}, year = {2018}, language = {en} } @article{GeorgievGrafmuellerBlegeretal.2018, author = {Georgiev, Vasil N. and Grafm{\"u}ller, Andrea and Bl{\´e}ger, David and Hecht, Stefan and Kunstmann, Sonja and Barbirz, Stefanie and Lipowsky, Reinhard and Dimova, Rumiana}, title = {Area increase and budding in giant vesicles triggered by light}, series = {Advanced science}, volume = {5}, journal = {Advanced science}, number = {8}, publisher = {Wiley}, address = {Hoboken}, issn = {2198-3844}, doi = {10.1002/advs.201800432}, pages = {9}, year = {2018}, abstract = {Biomembranes are constantly remodeled and in cells, these processes are controlled and modulated by an assortment of membrane proteins. Here, it is shown that such remodeling can also be induced by photoresponsive molecules. The morphological control of giant vesicles in the presence of a water-soluble ortho-tetrafluoroazobenzene photoswitch (F-azo) is demonstrated and it is shown that the shape transformations are based on an increase in membrane area and generation of spontaneous curvature. The vesicles exhibit budding and the buds can be retracted by using light of a different wavelength. In the presence of F-azo, the membrane area can increase by more than 5\% as assessed from vesicle electrodeformation. To elucidate the underlying molecular mechanism and the partitioning of F-azo in the membrane, molecular dynamics simulations are employed. Comparison with theoretically calculated shapes reveals that the budded shapes are governed by curvature elasticity, that the spontaneous curvature can be decomposed into a local and a nonlocal contribution, and that the local spontaneous curvature is about 1/(2.5 mu m). The results show that exo- and endocytotic events can be controlled by light and that these photoinduced processes provide an attractive method to change membrane area and morphology.}, language = {en} } @article{ClahsenPaulmannBuddetal.2018, author = {Clahsen, Harald and Paulmann, Silke and Budd, Mary-Jane and Barry, Christopher}, title = {Morphological encoding beyond slots and fillers}, series = {PLoS one}, volume = {13}, journal = {PLoS one}, number = {7}, publisher = {PLoS}, address = {San Fransisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0199897}, pages = {16}, year = {2018}, abstract = {One important organizational property of morphology is competition. Different means of expression are in conflict with each other for encoding the same grammatical function. In the current study, we examined the nature of this control mechanism by testing the formation of comparative adjectives in English during language production. Event-related brain potentials (ERPs) were recorded during cued silent production, the first study of this kind for comparative adjective formation. We specifically examined the ERP correlates of producing synthetic relative to analytic comparatives, e.g. angriervs. more angry. A frontal, bilaterally distributed, enhanced negative-going waveform for analytic comparatives (vis-a-vis synthetic ones) emerged approximately 300ms after the (silent) production cue. We argue that this ERP effect reflects a control mechanism that constrains grammatically-based computational processes (viz. more comparative formation). We also address the possibility that this particular ERP effect may belong to a family of previously observed negativities reflecting cognitive control monitoring, rather than morphological encoding processes per se.}, language = {en} } @misc{LazurasBarkoukisLoukovitisetal.2018, author = {Lazuras, Lambros and Barkoukis, Vassilis and Loukovitis, Andreas and Brand, Ralf and Hudson, Andy and Mallia, Luca and Michaelides, Michalis and Muzi, Milena and Petroczi, Andrea and Zelli, Arnaldo}, title = {Corrigendum: "I Want It All, and I Want It Now": Lifetime Prevalence and Reasons for Using and Abstaining from Controlled Performance and Appearance Enhancing Substances (PAES) among Young Exercisers and Amateur Athletes in Five European Countries (Frontiers in psychology. - 8 (2017), 717.)}, series = {Frontiers in psychology}, volume = {9}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2018.01162}, pages = {4}, year = {2018}, language = {en} } @article{FilipovicGerkeFilipovicetal.2018, author = {Filipovic, Vilim and Gerke, Horst H. and Filipovic, Lana and Sommer, Michael}, title = {Quantifying subsurface lateral flow along sloping horizon boundaries in soil profiles of a hummocky ground moraine}, series = {Vadose zone journal}, volume = {17}, journal = {Vadose zone journal}, number = {1}, publisher = {Soil Science Society of America}, address = {Madison}, issn = {1539-1663}, doi = {10.2136/vzj2017.05.0106}, pages = {12}, year = {2018}, abstract = {Subsurface lateral flow in hillslope soils depends on lower permeability or texture-contrasting soil horizons. In the arable hummocky soil landscape, erosion processes caused glacial till appearance closer to the soil surface at upslope positions. The objective of this work was to quantify the potential for subsurface lateral flow depending on the erosion-affected spatial hydropedological complexity. The eroded Haplic Luvisol profile was studied due to the presence of a relatively dense C horizon that varied in depth, thickness, and sloping angle. A two-dimensional numerical modeling and sensitivity analysis for the saturated hydraulic conductivity (K-s) of the C horizon and the depth to C horizon (i.e., soil solum thickness) was performed for rainstorms in 2011 and 2012 using HYDRUS-2D. A K-s value of <2.5 cm d(-1) for the C horizon was required for lateral flow initiation. Lateral flow was (i) increasing with decreasing solum thickness, indicating an erosion-induced feedback on subsurface lateral flow, and (ii) dependent on the soil moisture regime prior to rainstorms. The effect of lateral flow on the movement of a conservative tracer was simulated in the form of a "virtual experiment". Simulation scenarios revealed only a relatively small lateral shift of the tracer plume caused by a local decoupling of water (already lateral) from subsequent tracer movement (still vertical). Longer term simulations suggested that, for the present conditions, lateral flow was limited mostly to occasional summer storm events. Even without considering preferential flow contribution to lateral flow, highly complex hydropedologic interactions are present in erosion-affected heterogeneous soil profiles.}, language = {en} } @article{TotzLoeberTotzetal.2018, author = {Totz, Sonja Juliana and L{\"o}ber, Jakob and Totz, Jan Frederik and Engel, Harald}, title = {Control of transversal instabilities in reaction-diffusion systems}, series = {New journal of physics : the open-access journal for physics}, volume = {20}, journal = {New journal of physics : the open-access journal for physics}, publisher = {IOP Publ. Ltd.}, address = {Bristol}, issn = {1367-2630}, doi = {10.1088/1367-2630/aabce5}, pages = {16}, year = {2018}, abstract = {In two-dimensional reaction-diffusion systems, local curvature perturbations on traveling waves are typically damped out and vanish. However, if the inhibitor diffuses much faster than the activator, transversal instabilities can arise, leading from flat to folded, spatio-temporally modulated waves and to spreading spiral turbulence. Here, we propose a scheme to induce or inhibit these instabilities via a spatio-temporal feedback loop. In a piecewise-linear version of the FitzHugh-Nagumo model, transversal instabilities and spiral turbulence in the uncontrolled system are shown to be suppressed in the presence of control, thereby stabilizing plane wave propagation. Conversely, in numerical simulations with the modified Oregonator model for the photosensitive Belousov-Zhabotinsky reaction, which does not exhibit transversal instabilities on its own, we demonstrate the feasibility of inducing transversal instabilities and study the emerging wave patterns in a well-controlled manner.}, language = {en} } @article{KwanbunjanPanprathipPhosatetal.2018, author = {Kwanbunjan, Karunee and Panprathip, Pornpimol and Phosat, Chanchira and Chumpathat, Noppanath and Wechjakwen, Naruemon and Puduang, Somchai and Auyyuenyong, Ratchada and Henkel, Ina and Schweigert, Florian J.}, title = {Association of retinol binding protein 4 and transthyretin with triglyceride levels and insulin resistance in rural thais with high type 2 diabetes risk}, series = {BMC Endocrine Disorders}, volume = {18}, journal = {BMC Endocrine Disorders}, publisher = {BMC}, address = {London}, issn = {1472-6823}, doi = {10.1186/s12902-018-0254-2}, pages = {7}, year = {2018}, abstract = {Background: Retinol binding protein 4 (RBP4), a protein secreted by adipocytes and bound in plasma to transthyretin (TTR), has been associated with obesity, the early phase of insulin resistance, metabolic syndrome, and type 2 diabetes mellitus. The objective of this study was to elucidate the relationship between RBP4, TTR, triglyceride (TG) and type 2 diabetes risk in rural Thailand. Results: RBP4 and TTR levels, as well as homeostatic model assessment of insulin resistance (HOMA-IR) values, were significantly elevated among subjects with high triglyceride levels (p < 0.01, p < 0.05, p < 0.05, respectively). Triglyceride levels correlated with RBP4 (r = 0.34, p < 0.001) and TTR (r= 0.26, p < 0.01) levels, as well as HOMA-IR values (r= 0.16, p < 0.05). After adjustment for age and gender, the risk of hypertriglyceridemia was 3.7 times greater (95\% Cl =1.42 -9.73, p = 0.008) in the highest RBP4 tertile as compared to the lowest tertile. Similarly, the highest TTR and HOMA-IR tertiles had greater risk of hypertriglyceridemia at 3.5 (95\% Cl = 1.30-9.20, p = 0.01) and 3.6 (95\% CI = 1.33- 9.58, p = 0.01) times higher than the respective lowest tertiles. The correlation between TTR and blood glucose was statistically significant (r 0.18, p < 0.05), but not found this relationship in RBP4. Conclusions: The associations of RBP4 and TTR with hypertriglyceridemia and insulin resistance may have important implications for the risk of heart disease and stroke.}, language = {en} } @article{CasadoLandaisPicardetal.2018, author = {Casado, Mathieu and Landais, Amaelle and Picard, Ghislain and M{\"u}nch, Thomas and Laepple, Thomas and Stenni, Barbara and Dreossi, Giuliano and Ekaykin, Alexey and Arnaud, Laurent and Genthon, Christophe and Touzeau, Alexandra and Masson-Delmotte, Valerie and Jouzel, Jean}, title = {Archival processes of the water stable isotope signal in East Antarctic ice cores}, series = {The Cryosphere : TC ; an interactive open access journal of the European Geosciences Union}, volume = {12}, journal = {The Cryosphere : TC ; an interactive open access journal of the European Geosciences Union}, number = {5}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1994-0416}, doi = {10.5194/tc-12-1745-2018}, pages = {1745 -- 1766}, year = {2018}, abstract = {The oldest ice core records are obtained from the East Antarctic Plateau. Water isotopes are key proxies to reconstructing past climatic conditions over the ice sheet and at the evaporation source. The accuracy of climate reconstructions depends on knowledge of all processes affecting water vapour, precipitation and snow isotopic compositions. Fractionation processes are well understood and can be integrated in trajectory-based Rayleigh distillation and isotope-enabled climate models. However, a quantitative understanding of processes potentially altering snow isotopic composition after deposition is still missing. In low-accumulation sites, such as those found in East Antarctica, these poorly constrained processes are likely to play a significant role and limit the interpretability of an ice core's isotopic composition. By combining observations of isotopic composition in vapour, precipitation, surface snow and buried snow from Dome C, a deep ice core site on the East Antarctic Plateau, we found indications of a seasonal impact of metamorphism on the surface snow isotopic signal when compared to the initial precipitation. Particularly in summer, exchanges of water molecules between vapour and snow are driven by the diurnal sublimation-condensation cycles. Overall, we observe in between precipitation events modification of the surface snow isotopic composition. Using high-resolution water isotopic composition profiles from snow pits at five Antarctic sites with different accumulation rates, we identified common patterns which cannot be attributed to the seasonal variability of precipitation. These differences in the precipitation, surface snow and buried snow isotopic composition provide evidence of post-deposition processes affecting ice core records in low-accumulation areas.}, language = {en} } @article{HodgkinsRichardsonDommainetal.2018, author = {Hodgkins, Suzanne B. and Richardson, Curtis J. and Dommain, Rene and Wang, Hongjun and Glaser, Paul H. and Verbeke, Brittany and Winkler, B. Rose and Cobb, Alexander R. and Rich, Virginia I. and Missilmani, Malak and Flanagan, Neal and Ho, Mengchi and Hoyt, Alison M. and Harvey, Charles F. and Vining, S. Rose and Hough, Moira A. and Moore, Tim R. and Richard, Pierre J. H. and De la Cruz, Florentino B. and Toufaily, Joumana and Hamdan, Rasha and Cooper, William T. and Chanton, Jeffrey P.}, title = {Tropical peatland carbon storage linked to global latitudinal trends in peat recalcitrance}, series = {Nature Communications}, volume = {9}, journal = {Nature Communications}, publisher = {Nature Publ. Group}, address = {London}, issn = {2041-1723}, doi = {10.1038/s41467-018-06050-2}, pages = {13}, year = {2018}, abstract = {Peatlands represent large terrestrial carbon banks. Given that most peat accumulates in boreal regions, where low temperatures and water saturation preserve organic matter, the existence of peat in (sub)tropical regions remains enigmatic. Here we examined peat and plant chemistry across a latitudinal transect from the Arctic to the tropics. Near-surface low-latitude peat has lower carbohydrate and greater aromatic content than near-surface high-latitude peat, creating a reduced oxidation state and resulting recalcitrance. This recalcitrance allows peat to persist in the (sub)tropics despite warm temperatures. Because we observed similar declines in carbohydrate content with depth in high-latitude peat, our data explain recent field-scale deep peat warming experiments in which catotelm (deeper) peat remained stable despite temperature increases up to 9 degrees C. We suggest that high-latitude deep peat reservoirs may be stabilized in the face of climate change by their ultimately lower carbohydrate and higher aromatic composition, similar to tropical peats.}, language = {en} } @article{RonasiFischerZimmermann2018, author = {Ronasi, Golnoush and Fischer, Martin H. and Zimmermann, Malte}, title = {Language and Arithmetic}, series = {Frontiers in psychology}, volume = {9}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2018.01524}, pages = {12}, year = {2018}, abstract = {We examined cross-domain semantic priming effects between arithmetic and language. We paired subtractions with their linguistic equivalent, exception phrases (EPs) with positive quantifiers (e.g., "everybody except John") while pairing additions with their own linguistic equivalent, EPs with negative quantifiers (e.g., "nobody except John"; Moltmann, 1995). We hypothesized that EPs with positive quantifiers prime subtractions and inhibit additions while EPs with negative quantifiers prime additions and inhibit subtractions. Furthermore, we expected similar priming and inhibition effects from arithmetic into semantics. Our design allowed for a bidirectional analysis by using one trial's target as the prime for the next trial. Two experiments failed to show significant priming effects in either direction. Implications and possible shortcomings are explored in the general discussion.}, language = {en} } @misc{PostolicaIorgaSavinetal.2018, author = {Postolica, Roxana and Iorga, Magdalena and Savin, Mihaela and Azoicai, Doina and Enea, Violeta}, title = {The utility of Leventhal's model in the analysis of the psycho-behavioral implications of familial cancer}, series = {Archives of Medical Science}, volume = {14}, journal = {Archives of Medical Science}, number = {5}, publisher = {Termedia publishing house LTD}, address = {Poznan}, issn = {1734-1922}, doi = {10.5114/aoms.2016.63149}, pages = {1144 -- 1154}, year = {2018}, abstract = {Introduction: We aim to highlight the utility of this model in the analysis of the psycho-behavioral implications of family cancer, presenting the scientific literature that used Leventhal's model as the theoretical framework of approach. Material and methods: A systematic search was performed in six databases (EBSCO, ScienceDirect, PubMed Central, ProQuest, Scopus, and Web of Science) with empirical studies published between 2006 and 2015 in English with regard to the Common Sense Model of Self-Regulation (CSMR) and familial/hereditary cancer. The key words used were: illness representations, common sense model, self regulatory model, familial/hereditary/genetic cancer, genetic cancer counseling. The selection of studies followed the PRISMA-P guidelines (Moher et al., 2009; Shamseer et al., 2015), which suggest a three-stage procedure. Results: Individuals create their own cognitive and emotional representation of the disease when their health is threatened, being influenced by the presence of a family history of cancer, causing them to adopt or not a salutogenetic behavior. Disease representations, particularly the cognitive ones, can be predictors of responses to health threats that determine different health behaviors. Age, family history of cancer, and worrying about the disease are factors associated with undergoing screening. No consensus has been reached as to which factors act as predictors of compliance with cancer screening programs. Conclusions: This model can generate interventions that are conceptually clear as well as useful in regulating the individuals' behaviors by reducing the risk of developing the disease and by managing as favorably as possible health and/or disease.}, language = {en} } @article{CestnikRosenblum2018, author = {Cestnik, Rok and Rosenblum, Michael}, title = {Inferring the phase response curve from observation of a continuously perturbed oscillator}, series = {Scientific reports}, volume = {8}, journal = {Scientific reports}, publisher = {Nature Publ. Group}, address = {London}, issn = {2045-2322}, doi = {10.1038/s41598-018-32069-y}, pages = {10}, year = {2018}, abstract = {Phase response curves are important for analysis and modeling of oscillatory dynamics in various applications, particularly in neuroscience. Standard experimental technique for determining them requires isolation of the system and application of a specifically designed input. However, isolation is not always feasible and we are compelled to observe the system in its natural environment under free-running conditions. To that end we propose an approach relying only on passive observations of the system and its input. We illustrate it with simulation results of an oscillator driven by a stochastic force.}, language = {en} } @misc{GianelliGentilucci2018, author = {Gianelli, Claudia and Gentilucci, Maurizio}, title = {Editorial: Reaching to Grasp Cognition: Analyzing Motor Behavior to Investigate Social Interactions}, series = {Frontiers in psychology}, volume = {9}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2018.01236}, pages = {2}, year = {2018}, language = {en} } @article{LehrDannowskiKalettkaetal.2018, author = {Lehr, Christian and Dannowski, Ralf and Kalettka, Thomas and Merz, Christoph and Schr{\"o}der, Boris and Steidl, J{\"o}rg and Lischeid, Gunnar}, title = {Detecting dominant changes in irregularly sampled multivariate water quality data sets}, series = {Hydrology and earth system sciences : HESS}, volume = {22}, journal = {Hydrology and earth system sciences : HESS}, number = {8}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1027-5606}, doi = {10.5194/hess-22-4401-2018}, pages = {4401 -- 4424}, year = {2018}, abstract = {Time series of groundwater and stream water quality often exhibit substantial temporal and spatial variability, whereas typical existing monitoring data sets, e.g. from environmental agencies, are usually characterized by relatively low sampling frequency and irregular sampling in space and/or time. This complicates the differentiation between anthropogenic influence and natural variability as well as the detection of changes in water quality which indicate changes in single drivers. We suggest the new term "dominant changes" for changes in multivariate water quality data which concern (1) multiple variables, (2) multiple sites and (3) long-term patterns and present an exploratory framework for the detection of such dominant changes in data sets with irregular sampling in space and time. Firstly, a non-linear dimension-reduction technique was used to summarize the dominant spatiotemporal dynamics in the multivariate water quality data set in a few components. Those were used to derive hypotheses on the dominant drivers influencing water quality. Secondly, different sampling sites were compared with respect to median component values. Thirdly, time series of the components at single sites were analysed for long-term patterns. We tested the approach with a joint stream water and groundwater data set quality consisting of 1572 samples, each comprising sixteen variables, sampled with a spatially and temporally irregular sampling scheme at 29 sites in northeast Germany from 1998 to 2009. The first four components were interpreted as (1) an agriculturally induced enhancement of the natural background level of solute concentration, (2) a redox sequence from reducing conditions in deep groundwater to post-oxic conditions in shallow groundwater and oxic conditions in stream water, (3) a mixing ratio of deep and shallow groundwater to the streamflow and (4) sporadic events of slurry application in the agricultural practice. Dominant changes were observed for the first two components. The changing intensity of the first component was interpreted as response to the temporal variability of the thickness of the unsaturated zone. A steady increase in the second component at most stream water sites pointed towards progressing depletion of the denitrification capacity of the deep aquifer.}, language = {en} } @article{KrupkovaSadowskaKamedaetal.2018, author = {Krupkova, Olga and Sadowska, Aleksandra and Kameda, Takuya and Hitzl, Wolfgang and Hausmann, Oliver Nic and Klasen, J{\"u}rgen and Wuertz-Kozak, Karin}, title = {p38 MaPK Facilitates crosstalk Between endoplasmic reticulum stress and IL-6 release in the intervertebral Disc}, series = {Frontiers in Immunology}, volume = {9}, journal = {Frontiers in Immunology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-3224}, doi = {10.3389/fimmu.2018.01706}, pages = {14}, year = {2018}, abstract = {Degenerative disc disease is associated with increased expression of pro-inflammatory cytokines in the intervertebral disc (IVD). However, it is not completely clear how inflammation arises in the IVD and which cellular compartments are involved in this process. Recently, the endoplasmic reticulum (ER) has emerged as a possible modulator of inflammation in age-related disorders. In addition, ER stress has been associated with the microenvironment of degenerated IVDs. Therefore, the aim of this study was to analyze the effects of ER stress on inflammatory responses in degenerated human IVDs and associated molecular mechanisms. Gene expression of ER stress marker GRP78 and pro-inflammatory cytokines IL-6, IL-8, IL-1 beta, and TNF-alpha was analyzed in human surgical IVD samples (n = 51, Pfirrmann grade 2-5). The expression of GRP78 positively correlated with the degeneration grade in lumbar IVDs and IL-6, but not with IL-1 beta and TNF-alpha. Another set of human surgical IVD samples (n = 25) was used to prepare primary cell cultures. ER stress inducer thapsigargin (Tg, 100 and 500 nM) activated gene and protein expression of IL-6 and induced phosphorylation of p38 MAPK. Both inhibition of p38 MAPK by SB203580 (10 mu M) and knockdown of ER stress effector CCAAT-enhancer-binding protein homologous protein (CHOP) reduced gene and protein expression of IL-6 in Tg-treated cells. Furthermore, the effects of an inflammatory microenvironment on ER stress were tested. TNF-alpha (5 and 10 ng/mL) did not activate ER stress, while IL-1 beta (5 and 10 ng/mL) activated gene and protein expression of GRP78, but did not influence [Ca2+](i) flux and expression of CHOP, indicating that pro-inflammatory cytokines alone may not induce ER stress in vivo. This study showed that IL-6 release in the IVD can be initiated following ER stress and that ER stress mediates IL-6 release through p38 MAPK and CHOP. Therapeutic targeting of ER stress response may reduce the consequences of the harsh microenvironment in degenerated IVD.}, language = {en} } @article{MuenchLaepple2018, author = {M{\"u}nch, Thomas and Laepple, Thomas}, title = {What climate signal is contained in decadal- to centennial-scale isotope variations from Antarctic ice cores?}, series = {Climate of the past : CP}, volume = {14}, journal = {Climate of the past : CP}, number = {12}, publisher = {Copernicus Gesellschaft mbH}, address = {G{\"o}ttingen}, issn = {1814-9324}, doi = {10.5194/cp-14-2053-2018}, pages = {2053 -- 2070}, year = {2018}, abstract = {Ice-core-based records of isotopic composition are a proxy for past temperatures and can thus provide information on polar climate variability over a large range of timescales. However, individual isotope records are affected by a multitude of processes that may mask the true temperature variability. The relative magnitude of climate and non-climate contributions is expected to vary as a function of timescale, and thus it is crucial to determine those temporal scales on which the actual signal dominates the noise. At present, there are no reliable estimates of this timescale dependence of the signal-to-noise ratio (SNR). Here, we present a simple method that applies spectral analyses to stable-isotope data from multiple cores to estimate the SNR, and the signal and noise variability, as a function of timescale. The method builds on separating the contributions from a common signal and from local variations and includes a correction for the effects of diffusion and time uncertainty. We apply our approach to firn-core arrays from Dronning Maud Land (DML) in East Antarctica and from the West Antarctic Ice Sheet (WAIS). For DML and decadal to multi-centennial timescales, we find an increase in the SNR by nearly 1 order of magnitude (similar to 0.2 at decadal and similar to 1.0 at multi-centennial scales). The estimated spectrum of climate variability also shows increasing variability towards longer timescales, contrary to what is traditionally inferred from single records in this region. In contrast, the inferred variability spectrum for WAIS stays close to constant over decadal to centennial timescales, and the results even suggest a decrease in SNR over this range of timescales. We speculate that these differences between DML and WAIS are related to differences in the spatial and temporal scales of the isotope signal, highlighting the potentially more homogeneous atmospheric conditions on the Antarctic Plateau in contrast to the marine-influenced conditions on WAIS. In general, our approach provides a methodological basis for separating local proxy variability from coherent climate variations, which is applicable to a large set of palaeoclimate records.}, language = {en} } @article{GrafMorenodelasHerasRuizetal.2018, author = {Graf, Lukas and Moreno-de-las-Heras, Mariano and Ruiz, Maurici and Calsamiglia, Aleix and Garc{\´i}a-Comendador, Juli{\´a}n and Fortesa, Josep and L{\´o}pez-Taraz{\´o}n, Jos{\´e} A. and Estrany, Joan}, title = {Accuracy assessment of digital terrain model dataset sources for hydrogeomorphological modelling in small mediterranean catchments}, series = {Remote sensing}, volume = {10}, journal = {Remote sensing}, number = {12}, publisher = {MDPI}, address = {Basel}, issn = {2072-4292}, doi = {10.3390/rs10122014}, pages = {26}, year = {2018}, abstract = {Digital terrain models (DTMs) are a fundamental source of information in Earth sciences. DTM-based studies, however, can contain remarkable biases if limitations and inaccuracies in these models are disregarded. In this work, four freely available datasets, including Shuttle Radar Topography Mission C-Band Synthetic Aperture Radar (SRTM C-SAR V3 DEM), Advanced Spaceborne Thermal Emission and Reflection Radiometer Global Digital Elevation Map (ASTER GDEM V2), and two nationwide airborne light detection and ranging (LiDAR)-derived DTMs (at 5-m and 1-m spatial resolution, respectively) were analysed in three geomorphologically contrasting, small (3-5 km2) catchments located in Mediterranean landscapes under intensive human influence (Mallorca Island, Spain). Vertical accuracy as well as the influence of each dataset's characteristics on hydrological and geomorphological modelling applicability were assessed by using ground-truth data, classic geometric and morphometric parameters, and a recently proposed index of sediment connectivity. Overall vertical accuracy—expressed as the root mean squared error (RMSE) and normalised median deviation (NMAD)—revealed the highest accuracy for the 1-m (RMSE = 1.55 m; NMAD = 0.44 m) and 5-m LiDAR DTMs (RMSE = 1.73 m; NMAD = 0.84 m). Vertical accuracy of the SRTM data was lower (RMSE = 6.98 m; NMAD = 5.27 m), but considerably higher than for the ASTER data (RMSE = 16.10 m; NMAD = 11.23 m). All datasets were affected by systematic distortions. Propagation of these errors and coarse horizontal resolution caused negative impacts on flow routing, stream network, and catchment delineation, and to a lower extent, on the distribution of slope values. These limitations should be carefully considered when applying DTMs for catchment hydrogeomorphological modelling.}, language = {en} } @article{BruneWilliamsMueller2018, author = {Brune, Sascha and Williams, Simon E. and M{\"u}ller, R. Dietmar}, title = {Oblique rifting: the rule, not the exception}, series = {Solid earth}, volume = {9}, journal = {Solid earth}, number = {5}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1869-9510}, doi = {10.5194/se-9-1187-2018}, pages = {1187 -- 1206}, year = {2018}, abstract = {Movements of tectonic plates often induce oblique deformation at divergent plate boundaries. This is in striking contrast with traditional conceptual models of rifting and rifted margin formation, which often assume 2-D deformation where the rift velocity is oriented perpendicular to the plate boundary. Here we quantify the validity of this assumption by analysing the kinematics of major continent-scale rift systems in a global plate tectonic reconstruction from the onset of Pangea breakup until the present day. We evaluate rift obliquity by joint examination of relative extension velocity and local rift trend using the script-based plate reconstruction software pyGPlates. Our results show that the global mean rift obliquity since 230 Ma amounts to 34 degrees with a standard deviation of 24 degrees, using the convention that the angle of obliquity is spanned by extension direction and rift trend normal. We find that more than similar to 70 \% of all rift segments exceeded an obliquity of 20 degrees demonstrating that oblique rifting should be considered the rule, not the exception. In many cases, rift obliquity and extension velocity increase during rift evolution (e.g. Australia-Antarctica, Gulf of California, South Atlantic, India-Antarctica), which suggests an underlying geodynamic correlation via obliquity-dependent rift strength. Oblique rifting produces 3-D stress and strain fields that cannot be accounted for in simplified 2-D plane strain analysis. We therefore highlight the importance of 3-D approaches in modelling, surveying, and interpretation of most rift segments on Earth where oblique rifting is the dominant mode of deformation.}, language = {en} } @misc{RandallJuengelRimannetal.2018, author = {Randall, Matthew J. and J{\"u}ngel, Astrid and Rimann, Markus and Wuertz-Kozak, Karin}, title = {Advances in the biofabrication of 3D Skin in vitro}, series = {Frontiers in Bioengineeringand Biotechnology}, volume = {6}, journal = {Frontiers in Bioengineeringand Biotechnology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {2296-4185}, doi = {10.3389/fbioe.2018.00154}, pages = {12}, year = {2018}, abstract = {The relevance for in vitro three-dimensional (3D) tissue culture of skin has been present for almost a century. From using skin biopsies in organ culture, to vascularized organotypic full-thickness reconstructed human skin equivalents, in vitro tissue regeneration of 3D skin has reached a golden era. However, the reconstruction of 3D skin still has room to grow and develop. The need for reproducible methodology, physiological structures and tissue architecture, and perfusable vasculature are only recently becoming a reality, though the addition of more complex structures such as glands and tactile corpuscles require advanced technologies. In this review, we will discuss the current methodology for biofabrication of 3D skin models and highlight the advantages and disadvantages of the existing systems as well as emphasize how new techniques can aid in the production of a truly physiologically relevant skin construct for preclinical innovation.}, language = {en} } @article{SkinnerCoulthardSchwanghartetal.2018, author = {Skinner, Christopher J. and Coulthard, Tom J. and Schwanghart, Wolfgang and Van De Wiel, Marco J. and Hancock, Greg}, title = {Global sensitivity analysis of parameter uncertainty in landscape evolution models}, series = {Geoscientific model development : an interactive open access journal of the European Geosciences Union}, volume = {11}, journal = {Geoscientific model development : an interactive open access journal of the European Geosciences Union}, number = {12}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1991-959X}, doi = {10.5194/gmd-11-4873-2018}, pages = {4873 -- 4888}, year = {2018}, abstract = {The evaluation and verification of landscape evolution models (LEMs) has long been limited by a lack of suitable observational data and statistical measures which can fully capture the complexity of landscape changes. This lack of data limits the use of objective function based evaluation prolific in other modelling fields, and restricts the application of sensitivity analyses in the models and the consequent assessment of model uncertainties. To overcome this deficiency, a novel model function approach has been developed, with each model function representing an aspect of model behaviour, which allows for the application of sensitivity analyses. The model function approach is used to assess the relative sensitivity of the CAESAR-Lisflood LEM to a set of model parameters by applying the Morris method sensitivity analysis for two contrasting catchments. The test revealed that the model was most sensitive to the choice of the sediment transport formula for both catchments, and that each parameter influenced model behaviours differently, with model functions relating to internal geomorphic changes responding in a different way to those relating to the sediment yields from the catchment outlet. The model functions proved useful for providing a way of evaluating the sensitivity of LEMs in the absence of data and methods for an objective function approach.}, language = {en} } @article{CoesfeldAndersonBaughetal.2018, author = {Coesfeld, Jacqueline and Anderson, Sharolyn J. and Baugh, Kimberly and Elvidge, Christopher D. and Schernthanner, Harald and Kyba, Christopher C. M.}, title = {Variation of Individual Location Radiance in VIIRS DNB Monthly Composite Images}, series = {Remote sensing}, volume = {10}, journal = {Remote sensing}, number = {12}, publisher = {MDPI}, address = {Basel}, issn = {2072-4292}, doi = {10.3390/rs10121964}, pages = {17}, year = {2018}, abstract = {With the growing size and use of night light time series from the Visible Infrared Imaging Radiometer Suite Day/Night Band (DNB), it is important to understand the stability of the dataset. All satellites observe differences in pixel values during repeat observations. In the case of night light data, these changes can be due to both environmental effects and changes in light emission. Here we examine the stability of individual locations of particular large scale light sources (e.g., airports and prisons) in the monthly composites of DNB data from April 2012 to September 2017. The radiances for individual pixels of most large light emitters are approximately normally distributed, with a standard deviation of typically 15-20\% of the mean. Greenhouses and flares, however, are not stable sources. We observe geospatial autocorrelation in the monthly variations for nearby sites, while the correlation for sites separated by large distances is small. This suggests that local factors contribute most to the variation in the pixel radiances and furthermore that averaging radiances over large areas will reduce the total variation. A better understanding of the causes of temporal variation would improve the sensitivity of DNB to lighting changes.}, language = {en} } @article{SchultzeSchmidt2018, author = {Schultze, Christiane and Schmidt, Bernd}, title = {Ring-closing-metathesis-based synthesis of annellated coumarins from 8-allylcoumarins}, series = {Beilstein journal of organic chemistry}, volume = {14}, journal = {Beilstein journal of organic chemistry}, publisher = {Beilstein-Institut zur F{\"o}rderung der Chemischen Wissenschaften}, address = {Frankfurt, Main}, issn = {1860-5397}, doi = {10.3762/bjoc.14.278}, pages = {2991 -- 2998}, year = {2018}, abstract = {8-Allylcoumarins are conveniently accessible through a microwave-promoted tandem Claisen rearrangement/Wittig olefination/cyclization sequence. They serve as a versatile platform for the annellation of five- to seven-membered rings using ring-closing olefin metathesis (RCM). Furano-, pyrano-, oxepino- and azepinocoumarins were synthesized from the same set of precursors using Ru-catalyzed double bond isomerizations and RCM in a defined order. One class of products, pyrano[2,3-f]chromene-2,8-diones, were inaccessible through direct RCM of an acrylate, but became available from the analogous allyl ether via an assisted tandem catalytic RCM/allylic oxidation sequence.}, language = {en} } @article{FischerShaki2018, author = {Fischer, Martin H. and Shaki, Samuel}, title = {Repeating Numbers Reduces Results: Violations of the Identity Axiom in Mental Arithmetic}, series = {Frontiers in psychology}, volume = {9}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2018.02453}, pages = {9}, year = {2018}, abstract = {Even simple mental arithmetic is fraught with cognitive biases. For example, adding repeated numbers (so-called tie problems, e.g., 2 + 2) not only has a speed and accuracy advantage over adding different numbers (e.g., 1 + 3) but may also lead to under-representation of the result relative to a standard value (Charras et al., 2012, 2014). Does the tie advantage merely reflect easier encoding or retrieval compared to non-ties, or also a distorted result representation? To answer this question, 47 healthy adults performed two tasks, both of which indicated under-representation of tie results: In a result-to-position pointing task (Experiment 1) we measured the spatial mapping of numbers and found a left-bias for tie compared to non-tie problems. In a result-to-line-length production task (Experiment 2) we measured the underlying magnitude representation directly and obtained shorter lines for tie-compared to non-tie problems. These observations suggest that the processing benefit of tie problems comes at the cost of representational reduction of result meaning. This conclusion is discussed in the context of a recent model of arithmetic heuristics and biases.}, language = {en} } @article{MarrucciZeilingerRibolinietal.2018, author = {Marrucci, Monica and Zeilinger, Gerold and Ribolini, Adriano and Schwanghart, Wolfgang}, title = {Origin of Knickpoints in an Alpine Context Subject to Different Perturbing Factors, Stura Valley, Maritime Alps (North-Western Italy)}, series = {Geosciences}, volume = {8}, journal = {Geosciences}, number = {12}, publisher = {MDPI}, address = {Basel}, issn = {2076-3263}, doi = {10.3390/geosciences8120443}, pages = {20}, year = {2018}, abstract = {Natural catchments are likely to show the existence of knickpoints in their river networks. The origin and genesis of the knickpoints can be manifold, considering that the present morphology is the result of the interactions of different factors such as tectonic movements, quaternary glaciations, river captures, variable lithology, and base-level changes. We analyzed the longitudinal profiles of the river channels in the Stura di Demonte Valley (Maritime Alps) to identify the knickpoints of such an alpine setting and to characterize their origins. The distribution and the geometry of stream profiles were used to identify the possible causes of the changes in stream gradients and to define zones with genetically linked knickpoints. Knickpoints are key geomorphological features for reconstructing the evolution of fluvial dissected basins, when the different perturbing factors affecting the ideally graded fluvial system have been detected. This study shows that even in a regionally small area, perturbations of river profiles are caused by multiple factors. Thus, attributing (automatically)-extracted knickpoints solely to one factor, can potentially lead to incomplete interpretations of catchment evolution.}, language = {en} } @article{BormanndeBritoCharchousietal.2018, author = {Bormann, Helge and de Brito, Mariana Madruga and Charchousi, Despoina and Chatzistratis, Dimitris and David, Amrei and Grosser, Paula Farina and Kebschull, Jenny and Konis, Alexandros and Koutalakis, Paschalis and Korali, Alkistis and Krauzig, Naomi and Meier, Jessica and Meliadou, Varvara and Meinhardt, Markus and Munnelly, Kieran and Stephan, Christiane and de Vos, Leon Frederik and Dietrich, J{\"o}rg and Tzoraki, Ourania}, title = {Impact of Hydrological Modellers' Decisions and Attitude on the Performance of a Calibrated Conceptual Catchment Model}, series = {Hydrology}, volume = {5}, journal = {Hydrology}, number = {4}, publisher = {MDPI}, address = {Basel}, issn = {2306-5338}, doi = {10.3390/hydrology5040064}, pages = {13}, year = {2018}, abstract = {In this study, 17 hydrologists with different experience in hydrological modelling applied the same conceptual catchment model (HBV) to a Greek catchment, using identical data and model code. Calibration was performed manually. Subsequently, the modellers were asked for their experience, their calibration strategy, and whether they enjoyed the exercise. The exercise revealed that there is considerable modellers' uncertainty even among the experienced modellers. It seemed to be equally important whether the modellers followed a good calibration strategy, and whether they enjoyed modelling. The exercise confirmed previous studies about the benefit of model ensembles: Different combinations of the simulation results (median, mean) outperformed the individual model simulations, while filtering the simulations even improved the quality of the model ensembles. Modellers' experience, decisions, and attitude, therefore, have an impact on the hydrological model application and should be considered as part of hydrological modelling uncertainty.}, language = {en} } @article{PuppeWannerSommer2018, author = {Puppe, Daniel and Wanner, Manfred and Sommer, Michael}, title = {Data on euglyphid testate amoeba densities, corresponding protozoic silicon pools, and selected soil parameters of initial and forested biogeosystems}, series = {Data in brief}, volume = {21}, journal = {Data in brief}, publisher = {Elsevier}, address = {Amsterdam}, issn = {2352-3409}, doi = {10.1016/j.dib.2018.10.164}, pages = {1697 -- 1703}, year = {2018}, abstract = {The dataset in the present article provides information on protozoic silicon (Si) pools represented by euglyphid testate amoebae (TA) in soils of initial and forested biogeosystems. Protozoic Si pools were calculated from densities of euglyphid TA shells and corresponding Si contents. The article also includes data on potential annual biosilicification rates of euglyphid TA at the examined sites. Furthermore, data on selected soil parameters (e.g., readily-available Si, soil pH) and site characteristics (e.g., soil groups, climate data) can be found. The data might be interesting for researchers focusing on biological processes in Si cycling in general and euglyphid TA and corresponding protozoic Si pools in particular.}, language = {en} } @article{LuReichetzederPrehnetal.2018, author = {Lu, Yong-Ping and Reichetzeder, Christoph and Prehn, Cornelia and von Websky, Karoline and Slowinski, Torsten and Chen, You-Peng and Yin, Liang-Hong and Kleuser, Burkhard and Yang, Xue-Song and Adamski, Jerzy and Hocher, Berthold}, title = {Fetal serum metabolites are independently associated with Gestational diabetes mellitus}, series = {Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry and pharmacology}, volume = {45}, journal = {Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry and pharmacology}, number = {2}, publisher = {Karger}, address = {Basel}, issn = {1015-8987}, doi = {10.1159/000487119}, pages = {625 -- 638}, year = {2018}, abstract = {Background/Aims: Gestational diabetes (GDM) might be associated with alterations in the metabolomic profile of affected mothers and their offspring. Until now, there is a paucity of studies that investigated both, the maternal and the fetal serum metabolome in the setting of GDM. Mounting evidence suggests that the fetus is not just passively affected by gestational disease but might play an active role in it. Metabolomic studies performed in maternal blood and fetal cord blood could help to better discern distinct fetal from maternal disease interactions. Methods: At the time of birth, serum samples from mothers and newborns (cord blood samples) were collected and screened for 163 metabolites utilizing tandem mass spectrometry. The cohort consisted of 412 mother/child pairs, including 31 cases of maternal GDM. Results: An initial non-adjusted analysis showed that eight metabolites in the maternal blood and 54 metabolites in the cord blood were associated with GDM. After Benjamini-Hochberg (BH) procedure and adjustment for confounding factors for GDM, fetal phosphatidylcholine acyl-alkyl C 32:1 and proline still showed an independent association with GDM. Conclusions: This study found metabolites in cord blood which were associated with GDM, even after adjustment for established risk factors of GDM. To the best of our knowledge, this is the first study demonstrating an independent association between fetal serum metabolites and maternal GDM. Our findings might suggest a potential effect of the fetal metabolome on maternal GDM. (c) 2018 The Author(s) Published by S. Karger AG, Basel}, language = {en} } @article{LukanMachensColletal.2018, author = {Lukan, Tjaša and Machens, Fabian and Coll, Anna and Baebler, Špela and Messerschmidt, Katrin and Gruden, Kristina}, title = {Plant X-tender}, series = {PLOS ONE}, volume = {13}, journal = {PLOS ONE}, number = {1}, publisher = {Public Library of Science}, address = {San Fransisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0190526}, pages = {19}, year = {2018}, abstract = {Cloning multiple DNA fragments for delivery of several genes of interest into the plant genome is one of the main technological challenges in plant synthetic biology. Despite several modular assembly methods developed in recent years, the plant biotechnology community has not widely adopted them yet, probably due to the lack of appropriate vectors and software tools. Here we present Plant X-tender, an extension of the highly efficient, scar-free and sequence-independent multigene assembly strategy AssemblX, based on overlap-depended cloning methods and rare-cutting restriction enzymes. Plant X-tender consists of a set of plant expression vectors and the protocols for most efficient cloning into the novel vector set needed for plant expression and thus introduces advantages of AssemblX into plant synthetic biology. The novel vector set covers different backbones and selection markers to allow full design flexibility. We have included ccdB counterselection, thereby allowing the transfer of multigene constructs into the novel vector set in a straightforward and highly efficient way. Vectors are available as empty backbones and are fully flexible regarding the orientation of expression cassettes and addition of linkers between them, if required. We optimised the assembly and subcloning protocol by testing different scar-less assembly approaches: the noncommercial SLiCE and TAR methods and the commercial Gibson assembly and NEBuilder HiFi DNA assembly kits. Plant X-tender was applicable even in combination with low efficient homemade chemically competent or electrocompetent Escherichia coli. We have further validated the developed procedure for plant protein expression by cloning two cassettes into the newly developed vectors and subsequently transferred them to Nicotiana benthamiana in a transient expression setup. Thereby we show that multigene constructs can be delivered into plant cells in a streamlined and highly efficient way. Our results will support faster introduction of synthetic biology into plant science.}, language = {en} } @misc{AyzelIzhitskiy2018, author = {Ayzel, Georgy and Izhitskiy, Alexander}, title = {Coupling physically based and data-driven models for assessing freshwater inflow into the Small Aral Sea}, series = {Innovative Water Resources Management in a Changing Environment - Understanding and Balancing Interactions between Humankind and Nature}, volume = {379}, journal = {Innovative Water Resources Management in a Changing Environment - Understanding and Balancing Interactions between Humankind and Nature}, editor = {Xu, Z Peng}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {2199-899X}, doi = {10.5194/piahs-379-151-2018}, pages = {151 -- 158}, year = {2018}, abstract = {The Aral Sea desiccation and related changes in hydroclimatic conditions on a regional level is a hot topic for past decades. The key problem of scientific research projects devoted to an investigation of modern Aral Sea basin hydrological regime is its discontinuous nature - the only limited amount of papers takes into account the complex runoff formation system entirely. Addressing this challenge we have developed a continuous prediction system for assessing freshwater inflow into the Small Aral Sea based on coupling stack of hydrological and data-driven models. Results show a good prediction skill and approve the possibility to develop a valuable water assessment tool which utilizes the power of classical physically based and modern machine learning models both for territories with complex water management system and strong water-related data scarcity. The source code and data of the proposed system is available on a Github page (https://github.com/SMASHIproject/IWRM2018).}, language = {en} } @article{GiulbudagianYeallandHoenzkeetal.2018, author = {Giulbudagian, Michael and Yealland, Guy and H{\"o}nzke, S. and Edlich, A. and Geisend{\"o}rfer, Birte and Kleuser, Burkhard and Hedtrich, Sarah and Calderon, Marcelo}, title = {Breaking the Barrier}, series = {Theranostics}, volume = {8}, journal = {Theranostics}, number = {2}, publisher = {Ivyspring International Publisher}, address = {Lake haven}, issn = {1838-7640}, doi = {10.7150/thno.21668}, pages = {450 -- 463}, year = {2018}, abstract = {Topical administration permits targeted, sustained delivery of therapeutics to human skin. Delivery to the skin, however, is typically limited to lipophilic molecules with molecular weight of < 500 Da, capable of crossing the stratum corneum. Nevertheless, there are indications protein delivery may be possible in barrier deficient skin, a condition found in several inflammatory skin diseases such as psoriasis, using novel nanocarrier systems. Methods: Water in water thermo-nanoprecipitation; dynamic light scattering; zeta potential measurement; nanoparticle tracking analysis; atomic force microscopy; cryogenic transmission electron microscopy; UV absorption; centrifugal separation membranes; bicinchoninic acid assay; circular dichroism; TNF alpha binding ELISA; inflammatory skin equivalent construction; human skin biopsies; immunohistochemistry; fluorescence microscopy; western blot; monocyte derived Langerhans cells; ELISA Results: Here, we report the novel synthesis of thermoresponsive nanogels (tNG) and the stable encapsulation of the anti-TNFa fusion protein etanercept (ETR) (similar to 150 kDa) without alteration to its structure, as well as temperature triggered release from the tNGs. Novel tNG synthesis without the use of organic solvents was conducted, permitting in situ encapsulation of protein during assembly, something that holds great promise for easy manufacture and storage. Topical application of ETR loaded tNGs to inflammatory skin equivalents or tape striped human skin resulted in efficient ETR delivery throughout the SC and into the viable epidermis that correlated with clear anti-inflammatory effects. Notably, effective ETR delivery depended on temperature triggered release following topical application. Conclusion: Together these results indicate tNGs hold promise as a biocompatible and easy to manufacture vehicle for stable protein encapsulation and topical delivery into barrier-deficient skin.}, language = {en} } @article{BlanchardHoffmannReiss2018, author = {Blanchard, Gilles and Hoffmann, Marc and Reiss, Markus}, title = {Early stopping for statistical inverse problems via truncated SVD estimation}, series = {Electronic journal of statistics}, volume = {12}, journal = {Electronic journal of statistics}, number = {2}, publisher = {Institute of Mathematical Statistics}, address = {Cleveland}, issn = {1935-7524}, doi = {10.1214/18-EJS1482}, pages = {3204 -- 3231}, year = {2018}, abstract = {We consider truncated SVD (or spectral cut-off, projection) estimators for a prototypical statistical inverse problem in dimension D. Since calculating the singular value decomposition (SVD) only for the largest singular values is much less costly than the full SVD, our aim is to select a data-driven truncation level (m) over cap is an element of {1, . . . , D} only based on the knowledge of the first (m) over cap singular values and vectors. We analyse in detail whether sequential early stopping rules of this type can preserve statistical optimality. Information-constrained lower bounds and matching upper bounds for a residual based stopping rule are provided, which give a clear picture in which situation optimal sequential adaptation is feasible. Finally, a hybrid two-step approach is proposed which allows for classical oracle inequalities while considerably reducing numerical complexity.}, language = {en} } @article{BlanchardCarpentierGutzeit2018, author = {Blanchard, Gilles and Carpentier, Alexandra and Gutzeit, Maurilio}, title = {Minimax Euclidean separation rates for testing convex hypotheses in R-d}, series = {Electronic journal of statistics}, volume = {12}, journal = {Electronic journal of statistics}, number = {2}, publisher = {Institute of Mathematical Statistics}, address = {Cleveland}, issn = {1935-7524}, doi = {10.1214/18-EJS1472}, pages = {3713 -- 3735}, year = {2018}, abstract = {We consider composite-composite testing problems for the expectation in the Gaussian sequence model where the null hypothesis corresponds to a closed convex subset C of R-d. We adopt a minimax point of view and our primary objective is to describe the smallest Euclidean distance between the null and alternative hypotheses such that there is a test with small total error probability. In particular, we focus on the dependence of this distance on the dimension d and variance 1/n giving rise to the minimax separation rate. In this paper we discuss lower and upper bounds on this rate for different smooth and non-smooth choices for C.}, language = {en} } @article{KoloraWeigertSaffarietal.2018, author = {Kolora, Sree Rohit Raj and Weigert, Anne and Saffari, Amin and Kehr, Stephanie and Walter Costa, Maria Beatriz and Spr{\"o}er, Cathrin and Indrischek, Henrike and Chintalapati, Manjusha and Lohse, Konrad and Doose, Gero and Overmann, J{\"o}rg and Bunk, Boyke and Bleidorn, Christoph and Grimm-Seyfarth, Annegret and Henle, Klaus and Nowick, Katja and Faria, Rui and Stadler, Peter F. and Schlegel, Martin}, title = {Divergent evolution in the genomes of closely related lacertids, Lacerta viridis and L. bilineata, and implications for speciation}, series = {GigaScience}, volume = {8}, journal = {GigaScience}, number = {2}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {2047-217X}, doi = {10.1093/gigascience/giy160}, pages = {15}, year = {2018}, abstract = {Background Lacerta viridis and Lacerta bilineata are sister species of European green lizards (eastern and western clades, respectively) that, until recently, were grouped together as the L. viridis complex. Genetic incompatibilities were observed between lacertid populations through crossing experiments, which led to the delineation of two separate species within the L. viridis complex. The population history of these sister species and processes driving divergence are unknown. We constructed the first high-quality de novo genome assemblies for both L. viridis and L. bilineata through Illumina and PacBio sequencing, with annotation support provided from transcriptome sequencing of several tissues. To estimate gene flow between the two species and identify factors involved in reproductive isolation, we studied their evolutionary history, identified genomic rearrangements, detected signatures of selection on non-coding RNA, and on protein-coding genes. Findings Here we show that gene flow was primarily unidirectional from L. bilineata to L. viridis after their split at least 1.15 million years ago. We detected positive selection of the non-coding repertoire; mutations in transcription factors; accumulation of divergence through inversions; selection on genes involved in neural development, reproduction, and behavior, as well as in ultraviolet-response, possibly driven by sexual selection, whose contribution to reproductive isolation between these lacertid species needs to be further evaluated. Conclusion The combination of short and long sequence reads resulted in one of the most complete lizard genome assemblies. The characterization of a diverse array of genomic features provided valuable insights into the demographic history of divergence among European green lizards, as well as key species differences, some of which are candidates that could have played a role in speciation. In addition, our study generated valuable genomic resources that can be used to address conservation-related issues in lacertids.}, language = {en} } @article{HellwigTattiSartorietal.2018, author = {Hellwig, Niels and Tatti, Dylan and Sartori, Giacomo and Anschlag, Kerstin and Graefe, Ulfert and Egli, Markus and Gobat, Jean-Michel and Broll, Gabriele}, title = {Modeling spatial patterns of humus forms in montane and subalpine forests}, series = {Sustainability}, volume = {11}, journal = {Sustainability}, number = {1}, publisher = {MDPI}, address = {Basel}, issn = {2071-1050}, doi = {10.3390/su11010048}, pages = {15}, year = {2018}, abstract = {Humus forms are a distinctive morphological indicator of soil organic matter decomposition. The spatial distribution of humus forms depends on environmental factors such as topography, climate and vegetation. In montane and subalpine forests, environmental influences show a high spatial heterogeneity, which is reflected by a high spatial variability of humus forms. This study aims at examining spatial patterns of humus forms and their dependence on the spatial scale in a high mountain forest environment (Val di Sole/Val di Rabbi, Trentino, Italian Alps). On the basis of the distributions of environmental covariates across the study area, we described humus forms at the local scale (six sampling sites), slope scale (60 sampling sites) and landscape scale (30 additional sampling sites). The local variability of humus forms was analyzed with regard to the ground cover type. At the slope and landscape scale, spatial patterns of humus forms were modeled applying random forests and ordinary kriging of the model residuals. The results indicate that the occurrence of the humus form classes Mull, Mullmoder, Moder, Amphi and Eroded Moder generally depends on the topographical position. Local-scale patterns are mostly related to micro-topography (local accumulation and erosion sites) and ground cover, whereas slope-scale patterns are mainly connected with slope exposure and elevation. Patterns at the landscape scale show a rather irregular distribution, as spatial models at this scale do not account for local to slope-scale variations of humus forms. Moreover, models at the slope scale perform distinctly better than at the landscape scale. In conclusion, the results of this study highlight that landscape-scale predictions of humus forms should be accompanied by local- and slope-scale studies in order to enhance the general understanding of humus form patterns.}, language = {en} } @article{HeinBarnickel2018, author = {Hein, Johannes and Barnickel, Katja}, title = {Replication of R-pronouns in German dialects}, series = {Zeitschrift f{\"u}r Sprachwissenschaft : ZS}, volume = {37}, journal = {Zeitschrift f{\"u}r Sprachwissenschaft : ZS}, number = {2}, publisher = {De Gruyter Mouton}, address = {Berlin}, issn = {0721-9067}, doi = {10.1515/zfs-2018-0009}, pages = {171 -- 204}, year = {2018}, abstract = {A considerable number of German dialects exhibit doubled R-pronouns with pronominal adverbs (dadamit, dadafur, dadagegen). At first sight, this type of in situ replication seems to be completely redundant since its occurrence is independent of R-pronoun extraction/movement. The main purpose of this paper is to account for (i) the difference between dialects with regard to replication of R-pronouns and (ii) why an (apparently redundant) process of replication occurs. Following Muller (2000a), who considers R-pronouns to be a repair phenomenon, we present an analysis in the framework of Optimality Theory. We argue that replication of R-pronouns is a consequence of different rankings of universal requirements like e.g. the Inclusiveness Condition, the Lexical Integrity Hypothesis and Antilocality and that the interaction of these constraints results in the occurrence of replication.}, language = {en} } @misc{BoehneKreitz2018, author = {B{\"o}hne, Sebastian and Kreitz, Christoph}, title = {Learning how to prove}, series = {Electronic proceedings in theoretical computer science}, journal = {Electronic proceedings in theoretical computer science}, number = {267}, publisher = {Open Publishing Association}, address = {Sydney}, issn = {2075-2180}, doi = {10.4204/EPTCS.267.1}, pages = {1 -- 18}, year = {2018}, abstract = {We have developed an alternative approach to teaching computer science students how to prove. First, students are taught how to prove theorems with the Coq proof assistant. In a second, more difficult, step students will transfer their acquired skills to the area of textbook proofs. In this article we present a realisation of the second step. Proofs in Coq have a high degree of formality while textbook proofs have only a medium one. Therefore our key idea is to reduce the degree of formality from the level of Coq to textbook proofs in several small steps. For that purpose we introduce three proof styles between Coq and textbook proofs, called line by line comments, weakened line by line comments, and structure faithful proofs. While this article is mostly conceptional we also report on experiences with putting our approach into practise.}, language = {en} } @article{BachocBlanchardNeuvial2018, author = {Bachoc, Francois and Blanchard, Gilles and Neuvial, Pierre}, title = {On the post selection inference constant under restricted isometry properties}, series = {Electronic journal of statistics}, volume = {12}, journal = {Electronic journal of statistics}, number = {2}, publisher = {Institute of Mathematical Statistics}, address = {Cleveland}, issn = {1935-7524}, doi = {10.1214/18-EJS1490}, pages = {3736 -- 3757}, year = {2018}, abstract = {Uniformly valid confidence intervals post model selection in regression can be constructed based on Post-Selection Inference (PoSI) constants. PoSI constants are minimal for orthogonal design matrices, and can be upper bounded in function of the sparsity of the set of models under consideration, for generic design matrices. In order to improve on these generic sparse upper bounds, we consider design matrices satisfying a Restricted Isometry Property (RIP) condition. We provide a new upper bound on the PoSI constant in this setting. This upper bound is an explicit function of the RIP constant of the design matrix, thereby giving an interpolation between the orthogonal setting and the generic sparse setting. We show that this upper bound is asymptotically optimal in many settings by constructing a matching lower bound.}, language = {en} } @article{RusakTanentzapKlugetal.2018, author = {Rusak, James A. and Tanentzap, Andrew J. and Klug, Jennifer L. and Rose, Kevin C. and Hendricks, Susan P. and Jennings, Eleanor and Laas, Alo and Pierson, Donald C. and Ryder, Elizabeth and Smyth, Robyn L. and White, D. S. and Winslow, Luke A. and Adrian, Rita and Arvola, Lauri and de Eyto, Elvira and Feuchtmayr, Heidrun and Honti, Mark and Istvanovics, Vera and Jones, Ian D. and McBride, Chris G. and Schmidt, Silke Regina and Seekell, David and Staehr, Peter A. and Guangwei, Zhu}, title = {Wind and trophic status explain within and among-lake variability of algal biomass}, series = {Limnology and oceanography letters / ASLO, Association for the Sciences of Limnology and Oceanography}, volume = {3}, journal = {Limnology and oceanography letters / ASLO, Association for the Sciences of Limnology and Oceanography}, number = {6}, publisher = {Wiley}, address = {Hoboken}, issn = {2378-2242}, doi = {10.1002/lol2.10093}, pages = {409 -- 418}, year = {2018}, abstract = {Phytoplankton biomass and production regulates key aspects of freshwater ecosystems yet its variability and subsequent predictability is poorly understood. We estimated within-lake variation in biomass using high-frequency chlorophyll fluorescence data from 18 globally distributed lakes. We tested how variation in fluorescence at monthly, daily, and hourly scales was related to high-frequency variability of wind, water temperature, and radiation within lakes as well as productivity and physical attributes among lakes. Within lakes, monthly variation dominated, but combined daily and hourly variation were equivalent to that expressed monthly. Among lakes, biomass variability increased with trophic status while, within-lake biomass variation increased with increasing variability in wind speed. Our results highlight the benefits of high-frequency chlorophyll monitoring and suggest that predicted changes associated with climate, as well as ongoing cultural eutrophication, are likely to substantially increase the temporal variability of algal biomass and thus the predictability of the services it provides.}, language = {en} } @article{BeermannWestburyHofreiteretal.2018, author = {Beermann, Jan and Westbury, Michael V. and Hofreiter, Michael and Hilgers, Leon and Deister, Fabian and Neumann, Hermann and Raupach, Michael J.}, title = {Cryptic species in a well-known habitat}, series = {Scientific reports}, volume = {8}, journal = {Scientific reports}, publisher = {Nature Publ. Group}, address = {London}, issn = {2045-2322}, doi = {10.1038/s41598-018-25225-x}, pages = {26}, year = {2018}, abstract = {Taxonomy plays a central role in biological sciences. It provides a communication system for scientists as it aims to enable correct identification of the studied organisms. As a consequence, species descriptions should seek to include as much available information as possible at species level to follow an integrative concept of 'taxonomics'. Here, we describe the cryptic species Epimeria frankei sp. nov. from the North Sea, and also redescribe its sister species, Epimeria cornigera. The morphological information obtained is substantiated by DNA barcodes and complete nuclear 18S rRNA gene sequences. In addition, we provide, for the first time, full mitochondrial genome data as part of a metazoan species description for a holotype, as well as the neotype. This study represents the first successful implementation of the recently proposed concept of taxonomics, using data from high-throughput technologies for integrative taxonomic studies, allowing the highest level of confidence for both biodiversity and ecological research.}, language = {en} } @misc{Roschinsky2018, type = {Master Thesis}, author = {Roschinsky, Diane}, title = {Thomas Morus: Utopia}, series = {Copia - Potsdamer Anregungen f{\"u}r den Lateinunterricht}, volume = {2021}, journal = {Copia - Potsdamer Anregungen f{\"u}r den Lateinunterricht}, number = {1}, editor = {Forst, Alexandra}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {2748-6621}, doi = {10.25932/publishup-50468}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-504686}, school = {Universit{\"a}t Potsdam}, pages = {160}, year = {2018}, abstract = {In Thomas Morus' Utopia wird intensiv {\"u}ber die in einem idealen Staat herrschenden Verh{\"a}ltnisse nachgedacht. F{\"u}r den Lateinunterricht empfiehlt sich dieses neulateinische Werk, weil Sch{\"u}ler durch seine Lekt{\"u}re zum einen erkennen, dass die lateinische Sprache nach dem Untergang des r{\"o}mischen Reiches fortlebte, und weil sie zum anderen zu allgemeinen Reflexionen {\"u}ber vorbildhafte Gesellschaftsordnungen angeregt und f{\"u}r die dabei zu ber{\"u}cksichtigenden Aspekte sensibilisiert werden. So entsteht in ihnen ein Bewusstsein f{\"u}r die Grundfesten eines harmonischen Zusammenlebens. Das vorliegende Lekt{\"u}reheft bietet umfangreiches, didaktisch aufbereitetes Material, das Lateinsch{\"u}lern echtes Lesevergn{\"u}gen bereitet und das Lehrkr{\"a}fte ohne großen Aufwand im Unterricht einsetzen k{\"o}nnen. Diese Publikation schließt damit eine f{\"u}r die Utopia bislang bestehende L{\"u}cke und l{\"a}sst hoffen, dass das Werk k{\"u}nftig einen festen Platz im Lateinunterricht erh{\"a}lt.}, language = {de} } @inproceedings{Petsche2018, author = {Petsche, Hans-Joachim}, title = {In honour of Seymour Papert}, doi = {10.25932/publishup-49730}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-497302}, pages = {17}, year = {2018}, abstract = {Forth is nice and flexible but to a philosopher and teacher educator Logo is the more impressing language. Both are relatives of Lisp, but Forth has a reverse Polish notation where as Logo has an infix notation. Logo allows top down programming, Forth only bottom up. Logo enables recursive programming, Forth does not. Logo includes turtle graphics, Forth has nothing comparable. So what to do if you can't get Logo and have no information about its inner architecture? This should be a case of "empirical modelling": How can you model observable results of the behaviour of Logo in terms of Forth? The main steps to solve this problem are shown in the first part of the paper. The second part of the paper discusses the problem of modelling and shows that the modelling of making and the modelling of recognition have the same mathematical structure. So "empirical modelling" can also serve for modelling desired behaviour of technical systems. The last part of the paper will show that the heuristic potential of a problem which should be modeled is more important than the programming language. The Picasso construal shows, in a very simple way, how children of different ages can model emotional relations in human behaviour with a simple Logo system.}, language = {en} } @article{MummGodinaKozieletal.2018, author = {Mumm, Rebekka and Godina, Elena and Koziel, Slawomir and Musalek, Martin and Sedlak, Petr and Wittwer-Backofen, Ursula and Hess, Volker and Dasgupta, Parasmani and Henneberg, Maciej and Scheffler, Christiane}, title = {External skeletal robusticity of children and adolescents}, series = {Journal of biological and clinical anthropology}, volume = {74}, journal = {Journal of biological and clinical anthropology}, number = {5}, publisher = {Schweizerbart}, address = {Stuttgart}, issn = {0003-5548}, doi = {10.1127/anthranz/2018/0826}, pages = {383 -- 391}, year = {2018}, abstract = {Background: In our modern world, the way of life in nutritional and activity behaviour has changed. As a consequence, parallel trends of an epidemic of overweight and a decline in external skeletal robusticity are observed in children and adolescents. Aim: We aim to develop reference centiles for external skeletal robusticity of European girls and boys aged 0 to 18 years using the Frame Index as an indicator and identify population specific age-related patterns. Methods: We analysed cross-sectional \& longitudinal data on body height and elbow breadth of boys and girls from Europe (0-18 years, n = 41.679), India (7-18 years, n = 3.297) and South Africa (3-18 years, n = 4.346). As an indicator of external skeletal robusticity Frame Index after Frisancho (1990) was used. We developed centiles for boys and girls using the LMS-method and its extension. Results: Boys have greater external skeletal robusticity than girls. Whereas in girls Frame Index decreases continuously during growth, an increase of Frame Index from 12 to 16 years in European boys can be observed. Indian and South African boys are almost similar in Frame Index to European boys. In girls, the pattern is slightly different. Whereas South African girls are similar to European girls, Indian girls show a lesser external skeletal robusticity. Conclusion: Accurate references for external skeletal robusticity are needed to evaluate if skeletal development is adequate per age. They should be used to monitor effects of changes in way of life and physical activity levels in children and adolescents to avoid negative health outcomes like osteoporosis and arthrosis.}, language = {en} } @article{WetzelKempkaKuehn2018, author = {Wetzel, Maria and Kempka, Thomas and K{\"u}hn, Michael}, title = {Quantifying rock weakening due to decreasing calcite mineral content by numerical simulations}, series = {Materials}, volume = {11}, journal = {Materials}, number = {4}, publisher = {MDPI}, address = {Basel}, issn = {1996-1944}, doi = {10.3390/ma11040542}, pages = {19}, year = {2018}, abstract = {The quantification of changes in geomechanical properties due to chemical reactions is of paramount importance for geological subsurface utilisation, since mineral dissolution generally reduces rock stiffness. In the present study, the effective elastic moduli of two digital rock samples, the Fontainebleau and Bentheim sandstones, are numerically determined based on micro-CT images. Reduction in rock stiffness due to the dissolution of 10\% calcite cement by volume out of the pore network is quantified for three synthetic spatial calcite distributions (coating, partial filling and random) using representative sub-cubes derived from the digital rock samples. Due to the reduced calcite content, bulk and shear moduli decrease by 34\% and 38\% in maximum, respectively. Total porosity is clearly the dominant parameter, while spatial calcite distribution has a minor impact, except for a randomly chosen cement distribution within the pore network. Moreover, applying an initial stiffness reduced by 47\% for the calcite cement results only in a slightly weaker mechanical behaviour. Using the quantitative approach introduced here substantially improves the accuracy of predictions in elastic rock properties compared to general analytical methods, and further enables quantification of uncertainties related to spatial variations in porosity and mineral distribution.}, language = {en} } @article{ZemellaThoringHoffmeisteretal.2018, author = {Zemella, Anne and Thoring, Lena and Hoffmeister, Christian and Samalikova, Maria and Ehren, Patricia and W{\"u}stenhagen, Doreen Anja and Kubick, Stefan}, title = {Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin}, series = {Scientific reports}, volume = {8}, journal = {Scientific reports}, publisher = {Nature Publ. Group}, address = {London}, issn = {2045-2322}, doi = {10.1038/s41598-018-26936-x}, pages = {12}, year = {2018}, abstract = {As one of the most complex post-translational modification, glycosylation is widely involved in cell adhesion, cell proliferation and immune response. Nevertheless glycoproteins with an identical polypeptide backbone mostly differ in their glycosylation patterns. Due to this heterogeneity, the mapping of different glycosylation patterns to their associated function is nearly impossible. In the last years, glycoengineering tools including cell line engineering, chemoenzymatic remodeling and site-specific glycosylation have attracted increasing interest. The therapeutic hormone erythropoietin (EPO) has been investigated in particular by various groups to establish a production process resulting in a defined glycosylation pattern. However commercially available recombinant human EPO shows batch-to-batch variations in its glycoforms. Therefore we present an alternative method for the synthesis of active glycosylated EPO with an engineered O-glycosylation site by combining eukaryotic cell-free protein synthesis and site-directed incorporation of non-canonical amino acids with subsequent chemoselective modifications.}, language = {en} } @article{SallenHirschmannHerrmann2018, author = {Sallen, Jeffrey and Hirschmann, Florian and Herrmann, Christian}, title = {Evaluation and Adaption of the Trier Inventory for Chronic Stress (TICS) for Assessment in Competitive Sports}, series = {Frontiers in psychology}, volume = {9}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2018.00308}, pages = {12}, year = {2018}, abstract = {The demands of a career in competitive sports can lead to chronic stress perception among athletes if there is a non-conformity of requirements and available coping resources. The Trier Inventory for Chronic Stress (TICS) (Schulz et al., 2004) is said to be thoroughly validated. Nevertheless, it has not yet been subjected to a confirmatory factor analysis. The present study aims (1) to evaluate the factorial validity of the TICS within the context of competitive sports and (2) to adapt a short version (TICS-36). The total sample consisted of 564 athletes (age in years: M = 19.1, SD = 3.70). The factor structure of the original TICS did not adequately fit the present data, whereas the short version presented a satisfactory fit. The results indicate that the TICS-36 is an economical instrument for gathering interpretable information about chronic stress. For assessment in competitive sports with TICS-36, we generated overall and gender-specific norm values.}, language = {en} } @article{DonatLourencoPaolinietal.2018, author = {Donat, Stefan and Lourenco, Marta Sofia Rocha and Paolini, Alessio and Otten, Cecile and Renz, Marc and Abdelilah-Seyfried, Salim}, title = {Heg1 and Ccm1/2 proteins control endocardial mechanosensitivity during zebrafish valvulogenesis}, series = {eLife}, volume = {7}, journal = {eLife}, publisher = {eLife Sciences Publications}, address = {Cambridge}, issn = {2050-084X}, doi = {10.7554/eLife.28939}, pages = {22}, year = {2018}, abstract = {Endothelial cells respond to different levels of fluid shear stress through adaptations of their mechanosensitivity. Currently, we lack a good understanding of how this contributes to sculpting of the cardiovascular system. Cerebral cavernous malformation (CCM) is an inherited vascular disease that occurs when a second somatic mutation causes a loss of CCM1/KRIT1, CCM2, or CCM3 proteins. Here, we demonstrate that zebrafish Krit1 regulates the formation of cardiac valves. Expression of heg1, which encodes a binding partner of Krit1, is positively regulated by blood-flow. In turn, Heg1 stabilizes levels of Krit1 protein, and both Heg1 and Krit1 dampen expression levels of klf2a, a major mechanosensitive gene. Conversely, loss of Krit1 results in increased expression of klf2a and notch1b throughout the endocardium and prevents cardiac valve leaflet formation. Hence, the correct balance of blood-flow-dependent induction and Krit1 protein mediated repression of klf2a and notch1b ultimately shapes cardiac valve leaflet morphology.}, language = {en} } @article{OlmerEngelsUsmanetal.2018, author = {Olmer, Ruth and Engels, Lena and Usman, Abdulai and Menke, Sandra and Malik, Muhammad Nasir Hayat and Pessler, Frank and Goehring, Gudrun and Bornhorst, Dorothee and Bolten, Svenja and Abdelilah-Seyfried, Salim and Scheper, Thomas and Kempf, Henning and Zweigerdt, Robert and Martin, Ulrich}, title = {Differentiation of Human Pluripotent Stem Cells into Functional Endothelial Cells in Scalable Suspension Culture}, series = {Stem Cell Reports}, volume = {10}, journal = {Stem Cell Reports}, number = {5}, publisher = {Springer}, address = {New York}, issn = {2213-6711}, doi = {10.1016/j.stemcr.2018.03.017}, pages = {16}, year = {2018}, abstract = {Endothelial cells (ECs) are involved in a variety of cellular responses. As multifunctional components of vascular structures, endothelial (progenitor) cells have been utilized in cellular therapies and are required as an important cellular component of engineered tissue constructs and in vitro disease models. Although primary ECs from different sources are readily isolated and expanded, cell quantity and quality in terms of functionality and karyotype stability is limited. ECs derived from human induced pluripotent stem cells (hiPSCs) represent an alternative and potentially superior cell source, but traditional culture approaches and 2D differentiation protocols hardly allow for production of large cell numbers. Aiming at the production of ECs, we have developed a robust approach for efficient endothelial differentiation of hiPSCs in scalable suspension culture. The established protocol results in relevant numbers of ECs for regenerative approaches and industrial applications that show in vitro proliferation capacity and a high degree of chromosomal stability.}, language = {en} } @article{TaronLellBarlowetal.2018, author = {Taron, Ulrike H. and Lell, Moritz and Barlow, Axel and Paijmans, Johanna L. A.}, title = {Testing of Alignment Parameters for Ancient Samples}, series = {Genese}, volume = {9}, journal = {Genese}, number = {3}, publisher = {MDPI}, address = {Basel}, issn = {2073-4425}, doi = {10.3390/genes9030157}, pages = {12}, year = {2018}, abstract = {High-throughput sequence data retrieved from ancient or other degraded samples has led to unprecedented insights into the evolutionary history of many species, but the analysis of such sequences also poses specific computational challenges. The most commonly used approach involves mapping sequence reads to a reference genome. However, this process becomes increasingly challenging with an elevated genetic distance between target and reference or with the presence of contaminant sequences with high sequence similarity to the target species. The evaluation and testing of mapping efficiency and stringency are thus paramount for the reliable identification and analysis of ancient sequences. In this paper, we present 'TAPAS', (Testing of Alignment Parameters for Ancient Samples), a computational tool that enables the systematic testing of mapping tools for ancient data by simulating sequence data reflecting the properties of an ancient dataset and performing test runs using the mapping software and parameter settings of interest. We showcase TAPAS by using it to assess and improve mapping strategy for a degraded sample from a banded linsang (Prionodon linsang), for which no closely related reference is currently available. This enables a 1.8-fold increase of the number of mapped reads without sacrificing mapping specificity. The increase of mapped reads effectively reduces the need for additional sequencing, thus making more economical use of time, resources, and sample material.}, language = {en} } @article{MartinCreuzburgMassierWacker2018, author = {Martin-Creuzburg, Dominik and Massier, Tamara and Wacker, Alexander}, title = {Sex-Specific differences in essential lipid requirements of Daphnia magna}, series = {Frontiers in Ecology and Evolution}, volume = {6}, journal = {Frontiers in Ecology and Evolution}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {2296-701X}, doi = {10.3389/fevo.2018.00089}, pages = {14}, year = {2018}, abstract = {Sex-specific differences in nutritional requirements may crucially influence the performances of the sexes, which may have implications for sexual reproduction and thus is of great ecological and evolutionary interest. In the freshwater model species Daphnia magna, essential lipid requirements have been extensively studied. Dietary deficiencies in sterols and polyunsaturated fatty acids (PUFA) have been shown to constrain somatic growth and parthenogenetic reproduction of female Daphnia. In contrast, nutrient requirements of male Daphnia have not been studied yet. Supplementation experiments were conducted to investigate differences in sterol (cholesterol) and PUFA (eicosapentaenoic acid, EPA) requirements between female and male D. magna. Thresholds for sterol-limited juvenile growth were higher in females than in males, suggesting that females are more susceptible to dietary sterol deficiencies than males. Sex-specific differences in maximum somatic growth rates were evident primarily in the presence of dietary EPA; females could not exploit their generally higher growth potential in the absence of dietary PUFA. However, the thresholds for EPA-limited growth did not differ between sexes, suggesting that both sexes have similar dietary EPA requirements during juvenile growth. During a life history experiment, the gain in body dry mass was higher in females than in males, irrespective of food treatment. In both sexes, the gain in body dry mass increased significantly upon EPA supplementation, indicating that both sexes benefited from dietary EPA supply also later in life. However, the positive effects of EPA supplementation were most pronounced for female reproduction-related traits (i.e., clutch sizes, egg dry masses, and total dry mass investment in reproduction). The high maternal investment in reproduction resulted in a depletion of nutrients in female somata. In contrast, the comparatively low paternal investment in reproduction allowed for the accumulation of nutrients in male somata. We conclude that males are generally less susceptible to dietary nutrient deficiencies than females, because they can rely more on internal body stores. Our data suggest that the performances of the sexes are differentially influenced by lipid-mediated food quality, which may have consequences for sexual reproduction and thus the production of resting eggs and the maintenance of Daphnia populations.}, language = {en} } @article{ZurellvonWehrdenRoticsetal.2018, author = {Zurell, Damaris and von Wehrden, Henrik and Rotics, Shay and Kaatz, Michael and Gross, Helge and Schlag, Lena and Sch{\"a}fer, Merlin and Sapir, Nir and Turjeman, Sondra and Wikelski, Martin and Nathan, Ran and Jeltsch, Florian}, title = {Home range size and resource use of breeding and non-breeding white storks along a land use gradient}, series = {Frontiers in Ecology and Evolution}, volume = {6}, journal = {Frontiers in Ecology and Evolution}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {2296-701X}, doi = {10.3389/fevo.2018.00079}, pages = {11}, year = {2018}, abstract = {Biotelemetry is increasingly used to study animal movement at high spatial and temporal resolution and guide conservation and resource management. Yet, limited sample sizes and variation in space and habitat use across regions and life stages may compromise robustness of behavioral analyses and subsequent conservation plans. Here, we assessed variation in (i) home range sizes, (ii) home range selection, and (iii) fine-scale resource selection of white storks across breeding status and regions and test model transferability. Three study areas were chosen within the Central German breeding grounds ranging from agricultural to fluvial and marshland. We monitored GPS-locations of 62 adult white storks equipped with solar-charged GPS/3D-acceleration (ACC) transmitters in 2013-2014. Home range sizes were estimated using minimum convex polygons. Generalized linear mixed models were used to assess home range selection and fine-scale resource selection by relating the home ranges and foraging sites to Corine habitat variables and normalized difference vegetation index in a presence/pseudo-absence design. We found strong variation in home range sizes across breeding stages with significantly larger home ranges in non-breeding compared to breeding white storks, but no variation between regions. Home range selection models had high explanatory power and well predicted overall density of Central German white stork breeding pairs. Also, they showed good transferability across regions and breeding status although variable importance varied considerably. Fine-scale resource selection models showed low explanatory power. Resource preferences differed both across breeding status and across regions, and model transferability was poor. Our results indicate that habitat selection of wild animals may vary considerably within and between populations, and is highly scale dependent. Thereby, home range scale analyses show higher robustness whereas fine-scale resource selection is not easily predictable and not transferable across life stages and regions. Such variation may compromise management decisions when based on data of limited sample size or limited regional coverage. We thus recommend home range scale analyses and sampling designs that cover diverse regional landscapes and ensure robust estimates of habitat suitability to conserve wild animal populations.}, language = {en} } @article{RamageIrrgangMorgensternetal.2018, author = {Ramage, Justine Lucille and Irrgang, Anna Maria and Morgenstern, Anne and Lantuit, Hugues}, title = {Increasing coastal slump activity impacts the release of sediment and organic carbon into the Arctic Ocean}, series = {Biogeosciences}, volume = {15}, journal = {Biogeosciences}, number = {5}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1726-4170}, doi = {10.5194/bg-15-1483-2018}, pages = {1483 -- 1495}, year = {2018}, abstract = {Retrogressive thaw slumps (RTSs) are among the most active thermokarst landforms in the Arctic and deliver a large amount of material to the Arctic Ocean. However, their contribution to the organic carbon (OC) budget is unknown. We provide the first estimate of the contribution of RTSs to the nearshore OC budget of the Yukon Coast, Canada, and describe the evolution of coastal RTSs between 1952 and 2011 in this area. We (1) describe the evolution of RTSs between 1952 and 2011; (2) calculate the volume of eroded material and stocks of OC mobilized through slumping, including soil organic carbon (SOC) and dissolved organic carbon (DOC); and (3) estimate the OC fluxes mobilized through slumping between 1972 and 2011. We identified RTSs using high- resolution satellite imagery from 2011 and geocoded aerial photographs from 1952 and 1972. To estimate the volume of eroded material, we applied spline interpolation on an airborne lidar dataset acquired in July 2013. We inferred the stocks of mobilized SOC and DOC from existing related literature. Our results show a 73\% increase in the number of RTSs and 14\% areal expansion between 1952 and 2011. In the study area, RTSs displaced at least 16.6 x 10(6) m(3) of material, 53\% of which was ice, and mobilized 145.9 x 10(6) kg of OC. Between 1972 and 2011, 49 RTSs displaced 8.6 x 10(3) m(3) yr(-1) of material, adding 0.6\% to the OC flux released by coastal retreat along the Yukon Coast. Our results show that the contribution of RTSs to the nearshore OC budget is non-negligible and should be included when estimating the quantity of OC released from the Arctic coast to the ocean.}, language = {en} } @article{DiBaldassarreKreibichVorogushynetal.2018, author = {Di Baldassarre, Giuliano and Kreibich, Heidi and Vorogushyn, Sergiy and Aerts, Jeroen and Arnbjerg-Nielsen, Karsten and Barendrecht, Marlies and Bates, Paul and Borga, Marco and Botzen, Wouter and Bubeck, Philip and De Marchi, Bruna and Llasat, Carmen Maria and Mazzoleni, Maurizio and Molinari, Daniela and Mondino, Elena and Mard, Johanna and Petrucci, Olga and Scolobig, Anna and Viglione, Alberto and Ward, Philip J.}, title = {Hess Opinions: An interdisciplinary research agenda to explore the unintended consequences of structural flood protection}, series = {Hydrology and earth system sciences : HESS}, volume = {22}, journal = {Hydrology and earth system sciences : HESS}, number = {11}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1027-5606}, doi = {10.5194/hess-22-5629-2018}, pages = {5629 -- 5637}, year = {2018}, abstract = {One common approach to cope with floods is the implementation of structural flood protection measures, such as levees or flood-control reservoirs, which substantially reduce the probability of flooding at the time of implementation. Numerous scholars have problematized this approach. They have shown that increasing the levels of flood protection can attract more settlements and high-value assets in the areas protected by the new measures. Other studies have explored how structural measures can generate a sense of complacency, which can act to reduce preparedness. These paradoxical risk changes have been described as "levee effect", "safe development paradox" or "safety dilemma". In this commentary, we briefly review this phenomenon by critically analysing the intended benefits and unintended effects of structural flood protection, and then we propose an interdisciplinary research agenda to uncover these paradoxical dynamics of risk.}, language = {en} } @article{ZimmermannGrigorievPuretskiyetal.2018, author = {Zimmermann, Marc and Grigoriev, Dmitry and Puretskiy, Nikolay and B{\"o}ker, Alexander}, title = {Characteristics of microcontact printing with polyelectrolyte ink for the precise preparation of patches on silica particles}, series = {RSC Advances}, volume = {8}, journal = {RSC Advances}, number = {69}, publisher = {Royal Society of Chemistry}, address = {Cambridge}, issn = {2046-2069}, doi = {10.1039/c8ra07955b}, pages = {39241 -- 39247}, year = {2018}, abstract = {This publication demonstrates the abilities of a precise and straightforward microcontact printing approach for the preparation of patchy silica particles. In a broad particle size range, it is possible to finely tune the number and parameters of three-dimensional patches like diameter and thickness using only polyethyleneimine ink, poly(dimethoxysilane) as stamp material and a suitable release solvent.}, language = {en} } @article{BouchouleSchemmerHenkel2018, author = {Bouchoule, Isabelle and Schemmer, Max and Henkel, Carsten}, title = {Cooling phonon modes of a Bose condensate with uniform few body losses}, series = {Scipost Physics}, volume = {5}, journal = {Scipost Physics}, number = {5}, publisher = {Scipost foundation}, address = {Amsterdam}, issn = {2542-4653}, doi = {10.21468/SciPostPhys.5.5.043}, pages = {18}, year = {2018}, abstract = {We present a general analysis of the cooling produced by losses on condensates or quasi-condensates. We study how the occupations of the collective phonon modes evolve in time, assuming that the loss process is slow enough so that each mode adiabatically follows the decrease of the mean density. The theory is valid for any loss process whose rate is proportional to the jth power of the density, but otherwise spatially uniform. We cover both homogeneous gases and systems confined in a smooth potential. For a low-dimensional gas, we can take into account the modified equation of state due to the broadening of the cloud width along the tightly confined directions, which occurs for large interactions. We find that at large times, the temperature decreases proportionally to the energy scale mc(2), where m is the mass of the particles and c the sound velocity. We compute the asymptotic ratio of these two quantities for different limiting cases: a homogeneous gas in any dimension and a one-dimensional gas in a harmonic trap.}, language = {en} } @misc{KlugeGronau2018, author = {Kluge, Annette and Gronau, Norbert}, title = {Intentional forgetting in organizations}, series = {Frontiers in psychology}, volume = {9}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2018.00051}, pages = {17}, year = {2018}, abstract = {To cope with the already large, and ever increasing, amount of information stored in organizational memory, "forgetting," as an important human memory process, might be transferred to the organizational context. Especially in intentionally planned change processes (e.g., change management), forgetting is an important precondition to impede the recall of obsolete routines and adapt to new strategic objectives accompanied by new organizational routines. We first comprehensively review the literature on the need for organizational forgetting and particularly on accidental vs. intentional forgetting. We discuss the current state of the art of theory and empirical evidence on forgetting from cognitive psychology in order to infer mechanisms applicable to the organizational context. In this respect, we emphasize retrieval theories and the relevance of retrieval cues important for forgetting. Subsequently, we transfer the empirical evidence that the elimination of retrieval cues leads to faster forgetting to the forgetting of organizational routines, as routines are part of organizational memory. We then propose a classification of cues (context, sensory, business process-related cues) that are relevant in the forgetting of routines, and discuss a meta-cue called the "situational strength" cue, which is relevant if cues of an old and a new routine are present simultaneously. Based on the classification as business process-related cues (information, team, task, object cues), we propose mechanisms to accelerate forgetting by eliminating specific cues based on the empirical and theoretical state of the art. We conclude that in intentional organizational change processes, the elimination of cues to accelerate forgetting should be used in change management practices.}, language = {en} } @article{KretschmerCohenMatthiasetal.2018, author = {Kretschmer, Marlene and Cohen, Judah and Matthias, Vivien and Runge, Jakob and Coumou, Dim}, title = {The different stratospheric influence on cold-extremes in Eurasia and North America}, series = {npj Climate and Atmospheric Science}, volume = {1}, journal = {npj Climate and Atmospheric Science}, publisher = {Nature Publ. Group}, address = {London}, issn = {2397-3722}, doi = {10.1038/s41612-018-0054-4}, pages = {10}, year = {2018}, abstract = {The stratospheric polar vortex can influence the tropospheric circulation and thereby winter weather in the mid-latitudes. Weak vortex states, often associated with sudden stratospheric warmings (SSW), have been shown to increase the risk of cold-spells especially over Eurasia, but its role for North American winters is less clear. Using cluster analysis, we show that there are two dominant patterns of increased polar cap heights in the lower stratosphere. Both patterns represent a weak polar vortex but they are associated with different wave mechanisms and different regional tropospheric impacts. The first pattern is zonally symmetric and associated with absorbed upward-propagating wave activity, leading to a negative phase of the North Atlantic Oscillation (NAO) and cold-air outbreaks over northern Eurasia. This coupling mechanism is well-documented in the literature and is consistent with the downward migration of the northern annular mode (NAM). The second pattern is zonally asymmetric and linked to downward reflected planetary waves over Canada followed by a negative phase of the Western Pacific Oscillation (WPO) and cold-spells in Central Canada and the Great Lakes region. Causal effect network (CEN) analyses confirm the atmospheric pathways associated with this asymmetric pattern. Moreover, our findings suggest the reflective mechanism to be sensitive to the exact region of upward wave-activity fluxes and to be state-dependent on the strength of the vortex. Identifying the causal pathways that operate on weekly to monthly timescales can pave the way for improved sub-seasonal to seasonal forecasting of cold spells in the mid-latitudes.}, language = {en} } @article{OmidbakhshfardFujikuraOlasetal.2018, author = {Omidbakhshfard, Mohammad Amin and Fujikura, Ushio and Olas, Justyna Jadwiga and Xue, Gang-Ping and Balazadeh, Salma and Mueller-Roeber, Bernd}, title = {GROWTH-REGULATING FACTOR 9 negatively regulates arabidopsis leaf growth by controlling ORG3 and restricting cell proliferation in leaf primordia}, series = {PLoS Genetics : a peer-reviewed, open-access journal}, volume = {14}, journal = {PLoS Genetics : a peer-reviewed, open-access journal}, number = {7}, publisher = {PLoS}, address = {San Fransisco}, issn = {1553-7404}, doi = {10.1371/journal.pgen.1007484}, pages = {31}, year = {2018}, abstract = {Leaf growth is a complex process that involves the action of diverse transcription factors (TFs) and their downstream gene regulatory networks. In this study, we focus on the functional characterization of the Arabidopsis thaliana TF GROWTH-REGULATING FACTOR9 (GRF9) and demonstrate that it exerts its negative effect on leaf growth by activating expression of the bZIP TF OBP3-RESPONSIVE GENE 3 (ORG3). While grf9 knockout mutants produce bigger incipient leaf primordia at the shoot apex, rosette leaves and petals than the wild type, the sizes of those organs are reduced in plants overexpressing GRF9 (GRF9ox). Cell measurements demonstrate that changes in leaf size result from alterations in cell numbers rather than cell sizes. Kinematic analysis and 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay revealed that GRF9 restricts cell proliferation in the early developing leaf. Performing in vitro binding site selection, we identified the 6-base motif 5'-CTGACA-3' as the core binding site of GRF9. By global transcriptome profiling, electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) we identified ORG3 as a direct downstream, and positively regulated target of GRF9. Genetic analysis of grf9 org3 and GRF9ox org3 double mutants reveals that both transcription factors act in a regulatory cascade to control the final leaf dimensions by restricting cell number in the developing leaf.}, language = {en} } @article{KubinGuoKrolletal.2018, author = {Kubin, Markus and Guo, Meiyuan and Kroll, Thomas and Loechel, Heike and Kallman, Erik and Baker, Michael L. and Mitzner, Rolf and Gul, Sheraz and Kern, Jan and F{\"o}hlisch, Alexander and Erko, Alexei and Bergmann, Uwe and Yachandra, Vittal and Yano, Junko and Lundberg, Marcus and Wernet, Philippe}, title = {Probing the oxidation state of transition metal complexes}, series = {Chemical science}, volume = {9}, journal = {Chemical science}, number = {33}, publisher = {Royal Society of Chemistry}, address = {Cambridge}, issn = {2041-6520}, doi = {10.1039/c8sc00550h}, pages = {6813 -- 6829}, year = {2018}, abstract = {Transition metals in inorganic systems and metalloproteins can occur in different oxidation states, which makes them ideal redox-active catalysts. To gain a mechanistic understanding of the catalytic reactions, knowledge of the oxidation state of the active metals, ideally in operando, is therefore critical. L-edge X-ray absorption spectroscopy (XAS) is a powerful technique that is frequently used to infer the oxidation state via a distinct blue shift of L-edge absorption energies with increasing oxidation state. A unified description accounting for quantum-chemical notions whereupon oxidation does not occur locally on the metal but on the whole molecule and the basic understanding that L-edge XAS probes the electronic structure locally at the metal has been missing to date. Here we quantify how charge and spin densities change at the metal and throughout the molecule for both redox and core-excitation processes. We explain the origin of the L-edge XAS shift between the high-spin complexes Mn-II(acac)(2) and Mn-III(acac)(3) as representative model systems and use ab initio theory to uncouple effects of oxidation-state changes from geometric effects. The shift reflects an increased electron affinity of Mn-III in the core-excited states compared to the ground state due to a contraction of the Mn 3d shell upon core-excitation with accompanied changes in the classical Coulomb interactions. This new picture quantifies how the metal-centered core hole probes changes in formal oxidation state and encloses and substantiates earlier explanations. The approach is broadly applicable to mechanistic studies of redox-catalytic reactions in molecular systems where charge and spin localization/delocalization determine reaction pathways.}, language = {en} } @article{TanLiuSiemensmeyeretal.2018, author = {Tan, Li and Liu, Bing and Siemensmeyer, Konrad and Glebe, Ulrich and B{\"o}ker, Alexander}, title = {Synthesis of Polystyrene-Coated Superparamagnetic and Ferromagnetic Cobalt Nanoparticles}, series = {Polymers}, volume = {10}, journal = {Polymers}, number = {10}, publisher = {MDPI}, address = {Basel}, issn = {2073-4360}, doi = {10.3390/polym10101053}, pages = {18}, year = {2018}, abstract = {Polystyrene-coated cobalt nanoparticles (NPs) were synthesized through a dual-stage thermolysis of cobalt carbonyl (Co-2(CO)(8)). The amine end-functionalized polystyrene surfactants with varying molecular weight were prepared via atom-transfer radical polymerization technique. By changing the concentration of these polymeric surfactants, Co NPs with different size, size distribution, and magnetic properties were obtained. Transmission electron microscopy characterization showed that the size of Co NPs stabilized with lower molecular weight polystyrene surfactants (M-n = 2300 g/mol) varied from 12-22 nm, while the size of Co NPs coated with polystyrene of middle (M-n = 4500 g/mol) and higher molecular weight (M-n = 10,500 g/mol) showed little change around 20 nm. Magnetic measurements revealed that the small cobalt particles were superparamagnetic, while larger particles were ferromagnetic and self-assembled into 1-D chain structures. Thermogravimetric analysis revealed that the grafting density of polystyrene with lower molecular weight is high. To the best of our knowledge, this is the first study to obtain both superparamagnetic and ferromagnetic Co NPs by changing the molecular weight and concentration of polystyrene through the dual-stage decomposition method.}, language = {en} } @article{OttenKnoxBouldayetal.2018, author = {Otten, Cecile and Knox, Jessica and Boulday, Gwenola and Eymery, Mathias and Haniszewski, Marta and Neuenschwander, Martin and Radetzki, Silke and Vogt, Ingo and Haehn, Kristina and De Luca, Coralie and Cardoso, Cecile and Hamad, Sabri and Igual Gil, Carla and Roy, Peter and Albiges-Rizo, Corinne and Faurobert, Eva and von Kries, Jens P. and Campillos, Monica and Tournier-Lasserve, Elisabeth and Derry, William Brent and Abdelilah-Seyfried, Salim}, title = {Systematic pharmacological screens uncover novel pathways involved in cerebral cavernous malformations}, series = {EMBO molecular medicine}, volume = {10}, journal = {EMBO molecular medicine}, number = {10}, publisher = {Wiley}, address = {Hoboken}, issn = {1757-4676}, doi = {10.15252/emmm.201809155}, pages = {17}, year = {2018}, abstract = {Cerebral cavernous malformations (CCMs) are vascular lesions in the central nervous system causing strokes and seizures which currently can only be treated through neurosurgery. The disease arises through changes in the regulatory networks of endothelial cells that must be comprehensively understood to develop alternative, non-invasive pharmacological therapies. Here, we present the results of several unbiased small-molecule suppression screens in which we applied a total of 5,268 unique substances to CCM mutant worm, zebrafish, mouse, or human endothelial cells. We used a systems biology-based target prediction tool to integrate the results with the whole-transcriptome profile of zebrafish CCM2 mutants, revealing signaling pathways relevant to the disease and potential targets for small-molecule-based therapies. We found indirubin-3-monoxime to alleviate the lesion burden in murine preclinical models of CCM2 and CCM3 and suppress the loss-of-CCM phenotypes in human endothelial cells. Our multi-organism-based approach reveals new components of the CCM regulatory network and foreshadows novel small-molecule-based therapeutic applications for suppressing this devastating disease in patients.}, language = {en} } @article{HlawenkaSiemensmeyerWeschkeetal.2018, author = {Hlawenka, Peter and Siemensmeyer, Konrad and Weschke, Eugen and Varykhalov, Andrei and Sanchez-Barriga, Jaime and Shitsevalova, Natalya Y. and Dukhnenko, A. V. and Filipov, V. B. and Gabani, Slavomir and Flachbart, Karol and Rader, Oliver and Rienks, Emile D. L.}, title = {Samarium hexaboride is a trivial surface conductor}, series = {Nature Communications}, volume = {9}, journal = {Nature Communications}, publisher = {Nature Publ. Group}, address = {London}, issn = {2041-1723}, doi = {10.1038/s41467-018-02908-7}, pages = {7}, year = {2018}, abstract = {SmB6 is predicted to be the first member of the intersection of topological insulators and Kondo insulators, strongly correlated materials in which the Fermi level lies in the gap of a many-body resonance that forms by hybridization between localized and itinerant states. While robust, surface-only conductivity at low temperature and the observation of surface states at the expected high symmetry points appear to confirm this prediction, we find both surface states at the (100) surface to be topologically trivial. We find the (Gamma) over bar state to appear Rashba split and explain the prominent (X) over bar state by a surface shift of the many-body resonance. We propose that the latter mechanism, which applies to several crystal terminations, can explain the unusual surface conductivity. While additional, as yet unobserved topological surface states cannot be excluded, our results show that a firm connection between the two material classes is still outstanding.}, language = {en} } @article{HochreinMitchellSchulzetal.2018, author = {Hochrein, Lena and Mitchell, Leslie A. and Schulz, Karina and Messerschmidt, Katrin and M{\"u}ller-R{\"o}ber, Bernd}, title = {L-SCRaMbLE as a tool for light-controlled Cre-mediated recombination in yeast}, series = {Nature Communications}, volume = {9}, journal = {Nature Communications}, publisher = {Nature Publ. Group}, address = {London}, issn = {2041-1723}, doi = {10.1038/s41467-017-02208-6}, pages = {10}, year = {2018}, abstract = {The synthetic yeast genome constructed by the International Synthetic Yeast Sc2.0 consortium adds thousands of loxPsym recombination sites to all 16 redesigned chromosomes, allowing the shuffling of Sc2.0 chromosome parts by the Cre-loxP recombination system thereby enabling genome evolution experiments. Here, we present L-SCRaMbLE, a lightcontrolled Cre recombinase for use in the yeast Saccharomyces cerevisiae. L-SCRaMbLE allows tight regulation of recombinase activity with up to 179-fold induction upon exposure to red light. The extent of recombination depends on induction time and concentration of the chromophore phycocyanobilin (PCB), which can be easily adjusted. The tool presented here provides improved recombination control over the previously reported estradiol-dependent SCRaMbLE induction system, mediating a larger variety of possible recombination events in SCRaMbLE-ing a reporter plasmid. Thereby, L-SCRaMbLE boosts the potential for further customization and provides a facile application for use in the S. cerevisiae genome reengineering project Sc2.0 or in other recombination-based systems.}, language = {en} } @article{KruegerSchwarzeBaumannetal.2018, author = {Kr{\"u}ger, Stefanie and Schwarze, Michael and Baumann, Otto and G{\"u}nter, Christina and Bruns, Michael and K{\"u}bel, Christian and Szabo, Dorothee Vinga and Meinusch, Rafael and Bermudez, Veronica de Zea and Taubert, Andreas}, title = {Bombyx mori silk/titania/gold hybrid materials for photocatalytic water splitting}, series = {Beilstein journal of nanotechnology}, volume = {9}, journal = {Beilstein journal of nanotechnology}, publisher = {Beilstein-Institut zur F{\"o}rderung der Chemischen Wissenschaften}, address = {Frankfurt, Main}, issn = {2190-4286}, doi = {10.3762/bjnano.9.21}, pages = {187 -- 204}, year = {2018}, abstract = {The synthesis, structure, and photocatalytic water splitting performance of two new titania (TiO2)/gold(Au)/Bombyx mori silk hybrid materials are reported. All materials are monoliths with diameters of up to ca. 4.5 cm. The materials are macroscopically homogeneous and porous with surface areas between 170 and 210 m(2)/g. The diameter of the TiO2 nanoparticles (NPs) - mainly anatase with a minor fraction of brookite - and the Au NPs are on the order of 5 and 7-18 nm, respectively. Addition of poly(ethylene oxide) to the reaction mixture enables pore size tuning, thus providing access to different materials with different photocatalytic activities. Water splitting experiments using a sunlight simulator and a Xe lamp show that the new hybrid materials are effective water splitting catalysts and produce up to 30 mmol of hydrogen per 24 h. Overall the article demonstrates that the combination of a renewable and robust scaffold such as B. mori silk with a photoactive material provides a promising approach to new monolithic photocatalysts that can easily be recycled and show great potential for application in lightweight devices for green fuel production.}, language = {en} } @article{SchneiderGuenterTaubert2018, author = {Schneider, Matthias and G{\"u}nter, Christina and Taubert, Andreas}, title = {Co-deposition of a hydrogel/calcium phosphate hybrid layer on 3D printed poly(lactic acid) scaffolds via dip coating}, series = {Polymers}, volume = {10}, journal = {Polymers}, number = {3}, publisher = {MDPI}, address = {Basel}, issn = {2073-4360}, doi = {10.3390/polym10030275}, pages = {19}, year = {2018}, abstract = {The article describes the surface modification of 3D printed poly(lactic acid) (PLA) scaffolds with calcium phosphate (CP)/gelatin and CP/chitosan hybrid coating layers. The presence of gelatin or chitosan significantly enhances CP co-deposition and adhesion of the mineral layer on the PLA scaffolds. The hydrogel/CP coating layers are fairly thick and the mineral is a mixture of brushite, octacalcium phosphate, and hydroxyapatite. Mineral formation is uniform throughout the printed architectures and all steps (printing, hydrogel deposition, and mineralization) are in principle amenable to automatization. Overall, the process reported here therefore has a high application potential for the controlled synthesis of biomimetic coatings on polymeric biomaterials.}, language = {en} } @article{LopukhinaLaurinavichyuteLopukhinetal.2018, author = {Lopukhina, Anastasiya and Laurinavichyute, Anna and Lopukhin, Konstantin and Dragoy, Olga V.}, title = {The Mental Representation of Polysemy across Word Classes}, series = {Frontiers in psychology}, volume = {9}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2018.00192}, pages = {16}, year = {2018}, abstract = {Experimental studies on polysemy have come to contradictory conclusions on whether words with multiple senses are stored as separate or shared mental representations. The present study examined the semantic relatedness and semantic similarity of literal and non-literal (metonymic and metaphorical) senses of three word classes: nouns, verbs, and adjectives. Two methods were used: a psycholinguistic experiment and a distributional analysis of corpus data. In the experiment, participants were presented with 6-12 short phrases containing a polysemous word in literal, metonymic, or metaphorical senses and were asked to classify them so that phrases with the same perceived sense were grouped together. To investigate the impact of professional background on their decisions, participants were controlled for linguistic vs. non-linguistic education. For nouns and verbs, all participants preferred to group together phrases with literal and metonymic senses, but not any other pairs of senses. For adjectives, two pairs of senses were often grouped together: literal with metonymic, and metonymic with metaphorical. Participants with a linguistic background were more accurate than participants with non-linguistic backgrounds, although both groups shared principal patterns of sense classification. For the distributional analysis of corpus data, we used a semantic vector approach to quantify the similarity of phrases with literal, metonymic, and metaphorical senses in the corpora. We found that phrases with literal and metonymic senses had the highest degree of similarity for the three word classes, and that metonymic and metaphorical senses of adjectives had the highest degree of similarity among all word classes. These findings are in line with the experimental results. Overall, the results suggest that the mental representation of a polysemous word depends on its word class. In nouns and verbs, literal and metonymic senses are stored together, while metaphorical senses are stored separately; in adjectives, metonymic senses significantly overlap with both literal and metaphorical senses.}, language = {en} } @article{NeugartWiesnerReinholdFredeetal.2018, author = {Neugart, Susanne and Wiesner-Reinhold, Melanie and Frede, Katja and Jander, Elisabeth and Homann, Thomas and Rawel, Harshadrai Manilal and Schreiner, Monika and Baldermann, Susanne}, title = {Effect of Solid Biological Waste Compost on the Metabolite Profile of Brassica rapa ssp chinensis}, series = {Frontiers in plant science : FPLS}, volume = {9}, journal = {Frontiers in plant science : FPLS}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {1664-462X}, doi = {10.3389/fpls.2018.00305}, pages = {13}, year = {2018}, abstract = {Large quantities of biological waste are generated at various steps within the food production chain and a great utilization potential for this solid biological waste exists apart from the current main usage for the feedstuff sector. It remains unclear how the usage of biological waste as compost modulates plant metabolites. We investigated the effect of biological waste of the processing of coffee, aronia, and hop added to soil on the plant metabolite profile by means of liquid chromatography in pak choi sprouts. Here we demonstrate that the solid biological waste composts induced specific changes in the metabolite profiles and the changes are depending on the type of the organic residues and its concentration in soil. The targeted analysis of selected plant metabolites, associated with health beneficial properties of the Brassicaceae family, revealed increased concentrations of carotenoids (up to 3.2-fold) and decreased amounts of glucosinolates (up to 4.7-fold) as well as phenolic compounds (up to 1.5-fold).}, language = {en} } @article{OdongoSchlotzBaldermannetal.2018, author = {Odongo, Grace Akinyi and Schlotz, Nina and Baldermann, Susanne and Neugart, Susanne and Huyskens-Keil, Susanne and Ngwene, Benard and Trierweiler, Bernhard and Schreiner, Monika and Lamy, Evelyn}, title = {African Nightshade (Solanum scabrum Mill.)}, series = {Nutrients}, volume = {10}, journal = {Nutrients}, number = {10}, publisher = {MDPI}, address = {Basel}, issn = {2072-6643}, doi = {10.3390/nu10101532}, pages = {20}, year = {2018}, abstract = {Plant cultivation and processing may impact nutrient and phytochemical content of vegetables. The present study aimed at determining the influence of cultivation and processing on the health promoting capacity of African nightshade (Solanum scabrum Mill.) leaves, an indigenous vegetable, rich in nutrients and phytochemicals. Anti-genotoxicity against the human liver carcinogen aflatoxin B1 (AFB(1)) as determined by the comet assay and radical oxygen species (ROS) scavenging capacity of ethanolic and aqueous extracts were investigated in human derived liver (HepG2) cells. ROS scavenging activity was assessed using electron paramagnetic spin resonance and quantification of ARE/Nrf2 mediated gene expression. The cultivation was done under different environmental conditions. The processing included fermentation and cooking; postharvest ultraviolet irradiation (UV-C) treatment was also investigated. Overall, S. scabrum extracts showed strong health promoting potential, the highest potential was observed with the fermented extract, which showed a 60\% reduction of AFB(1) induced DNA damage and a 38\% reduction in FeSO4 induced oxidative stress. The content of total polyphenols, carotenoids and chlorophylls was indeed affected by cultivation and processing. Based on the present in vitro findings consumption of S. scabrum leaves could be further encouraged, preferentially after cooking or fermentation of the plant.}, language = {en} } @article{PathirajaAnghileriBurlandoetal.2018, author = {Pathiraja, Sahani Darschika and Anghileri, Daniela and Burlando, Paolo and Sharma, Ashish and Marshall, Lucy and Moradkhani, Hamid}, title = {Time-varying parameter models for catchments with land use change}, series = {Hydrology and earth system sciences : HESS}, volume = {22}, journal = {Hydrology and earth system sciences : HESS}, number = {5}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1027-5606}, doi = {10.5194/hess-22-2903-2018}, pages = {2903 -- 2919}, year = {2018}, abstract = {Rapid population and economic growth in Southeast Asia has been accompanied by extensive land use change with consequent impacts on catchment hydrology. Modeling methodologies capable of handling changing land use conditions are therefore becoming ever more important and are receiving increasing attention from hydrologists. A recently developed data-assimilation-based framework that allows model parameters to vary through time in response to signals of change in observations is considered for a medium-sized catchment (2880 km(2)) in northern Vietnam experiencing substantial but gradual land cover change. We investigate the efficacy of the method as well as the importance of the chosen model structure in ensuring the success of a time-varying parameter method. The method was used with two lumped daily conceptual models (HBV and HyMOD) that gave good-quality streamflow predictions during pre-change conditions. Although both time-varying parameter models gave improved streamflow predictions under changed conditions compared to the time-invariant parameter model, persistent biases for low flows were apparent in the HyMOD case. It was found that HyMOD was not suited to representing the modified baseflow conditions, resulting in extreme and unrealistic time-varying parameter estimates. This work shows that the chosen model can be critical for ensuring the time-varying parameter framework successfully models streamflow under changing land cover conditions. It can also be used to determine whether land cover changes (and not just meteorological factors) contribute to the observed hydrologic changes in retrospective studies where the lack of a paired control catchment precludes such an assessment.}, language = {en} } @article{KlopschBaldermannVossetal.2018, author = {Klopsch, Rebecca and Baldermann, Susanne and Voss, Alexander and Rohn, Sascha and Schreiner, Monika and Neugart, Susanne}, title = {Bread enriched with legume microgreens and leaves}, series = {Frontiers in chemistry}, volume = {6}, journal = {Frontiers in chemistry}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {2296-2646}, doi = {10.3389/fchem.2018.00322}, pages = {19}, year = {2018}, abstract = {Flavonoids, carotenoids, and chlorophylls were characterized in microgreens and leaves of pea (Pisum sativum) and lupin (Lupinus angustifolius) as these metabolites change during ontogeny. All metabolites were higher in the leaves for both species. Acylated quercetin and kaempferol sophorotrioses were predominant in pea. Genistein and malonylated chrysoeriol were predominant in lupin. Further, the impact of breadmaking on these metabolites using pea and lupin material of two ontogenetic stages as an added ingredient in wheat-based bread was assessed. In "pea microgreen bread" no decrease of quercetin was found with regard to the non-processed plant material. However kaempferol glycosides showed slight decreases induced by the breadmaking process in "pea microgreen bread" and "pea leaf bread." In "lupin microgreen bread" no decrease of genistein compared to the non-processed plant material was found. Chrysoeriol glycosides showed slight decreases induced by the breadmaking process in "lupin microgreen bread" and "lupin leaf bread." In all breads, carotenoids and chlorophylls were depleted however pheophytin formation was caused. Thus, pea and lupin microgreens and leaves are suitable, natural ingredients for enhancing health-promoting secondary plant metabolites in bread and may even be used to tailor bread for specific consumer health needs.}, language = {en} } @article{NennewitzThiedeBookhagen2018, author = {Nennewitz, Markus and Thiede, Rasmus C. and Bookhagen, Bodo}, title = {Fault activity, tectonic segmentation, and deformation pattern of the western Himalaya on Ma timescales inferred from landscape morphology}, series = {Lithosphere}, volume = {10}, journal = {Lithosphere}, number = {5}, publisher = {American Institute of Physics}, address = {Boulder}, issn = {1941-8264}, doi = {10.1130/L681.1}, pages = {632 -- 640}, year = {2018}, abstract = {The location and magnitude of Himalayan tectonic activity has been debated for decades, and several aspects remain unknown. For instance, the spatial distribution of crustal shortening that ultimately sustains Himalayan topography and the activity of major fault zones remain unknown at Ma timescales. In this study, we address the spatial deformation pattern in the data-scarce western Himalaya. We calculated catchment averaged, normalized river-steepness indices of non-glaciated drainage basins with tributary catchment areas between 5 and 200 km(2) (n = 2138). We analyzed the spatial distribution of the relative change of river steepness both along and across strike to gain information about the regional distribution of differential uplift pattern and relate this to the activity of distinctive fault segments. For our study area, we observe a positive correlation of averaged k(sn) values with long-term exhumation rates derived from previously published thermochronologic datasets combined with thermal modeling as well as with millennial timescale denudation rates based on cosmogenic nuclide dating. Our results indicate three tectono-geomorphic segments with distinctive landscape morphology, structural architecture, and fault geometry along the western Himalaya: Garhwal-Sutlej, Chamba, and Kashmir Himalaya (from east to west). Moreover, our data recognize distinctive fault segments showing varying thrust activity along strike of the Main Frontal Thrust, the Main Boundary Thrust, and in the vicinity of the steep topographic transition between the Lesser and Greater Himalaya. In this region, we relate out-of-sequence deformation along major basement thrust ramps, such as the Munsiari Thrust with deformation along a mid-crustal ramp along the basal decollement. We suggest that during the Quaternary, all major fault zones in the Western Himalaya experienced out-of-sequence faulting and have accommodated some portion of crustal shortening.}, language = {en} } @article{JobeLiBookhagenetal.2018, author = {Jobe, Jessica Ann Thompson and Li, Tao and Bookhagen, Bodo and Chen, Jie and Burbank, Douglas W.}, title = {Dating growth strata and basin fill by combining Al-26/Be-10 burial dating and magnetostratigraphy}, series = {Lithosphere}, volume = {10}, journal = {Lithosphere}, number = {6}, publisher = {American Institute of Physics}, address = {Boulder}, issn = {1941-8264}, doi = {10.1130/L727.1}, pages = {806 -- 828}, year = {2018}, abstract = {Cosmogenic burial dating enables dating of coarse-grained, Pliocene-Pleistocene sedimentary units that are typically difficult to date with traditional methods, such as magnetostratigraphy. In the actively deforming western Tarim Basin in NW China, Pliocene-Pleistocene conglomerates were dated at eight sites, integrating Al-26/Be-10 burial dating with previously published magnetostratigraphic sections. These samples were collected from growth strata on the flanks of growing folds and from sedimentary units beneath active faults to place timing constraints on the initiation of deformation of structures within the basin and on shortening rates on active faults. These new basin-fill and growthstrata ages document the late Neogene and Quaternary growth of the Pamir and Tian Shan orogens between >5 and 1 Ma and delineate the eastward propagation of deformation at rates up to 115 km/m.y. and basinward growth of both mountain belts at rates up to 12 km/m.y.}, language = {en} } @article{HeimEccardBairlein2018, author = {Heim, Wieland and Eccard, Jana and Bairlein, Franz}, title = {Migration phenology determines niche use of East Asian buntings (Emberizidae) during stopover}, series = {Current zoology}, volume = {64}, journal = {Current zoology}, number = {6}, publisher = {Oxford Univ. Press}, address = {Oxford}, issn = {1674-5507}, doi = {10.1093/cz/zoy016}, pages = {681 -- 692}, year = {2018}, abstract = {Stopover niche utilization of birds during migration has not gained much attention so far, since the majority of the studies focuses on breeding or wintering areas. However, stopover sites are crucial for migratory birds. They are often used by a multitude of species, which could lead to increased competition. In this work, we investigated niche use of 8 migratory and closely related Emberiza bunting species at a stopover site in Far East Russia, situated on the poorly studied East Asian fly-way. We used bird ringing data to evaluate morphological similarity as well as niche overlap on the trophic, spatial, and temporal dimension. Bill morphology was used as a proxy for their trophic niche. We were able to prove that a majority of the species occupies well-defined stopover niches on at least one of the dimensions. Niche breadth and niche overlap differ between spring and autumn season with higher overlap found during spring. Morphological differences are mostly related to overall size and wing pointedness. The temporal dimension is most important for segregation among the studied species. Furthermore, all species seem to exhibit a rather strict and consistent phenological pattern. Their occurrence at the study site is highly correlated with their geographic origin and the length of their migration route. We assume that buntings are able to use available resources opportunistically during stopover, while trying to follow a precise schedule in order to avoid competition and to ensure individual fitness.}, language = {en} } @article{HalamaGlodnyKonradSchmolkeetal.2018, author = {Halama, Ralf and Glodny, Johannes and Konrad-Schmolke, Matthias and Sudo, Masafumi}, title = {Rb-Sr and in situ Ar-40/Ar-39 dating of exhumation-related shearing and fluid-induced recrystallization in the Sesia zone (Western Alps, Italy)}, series = {Geosphere}, volume = {14}, journal = {Geosphere}, number = {4}, publisher = {American Institute of Physics}, address = {Boulder}, issn = {1553-040X}, doi = {10.1130/GES01521.1}, pages = {1425 -- 1450}, year = {2018}, abstract = {The Sesia zone in the Italian Western Alps is a piece of continental crust that has been subducted to eclogite-facies conditions and records a complex metamorphic history. The exact timing of events and the significance of geochronological information are debated due to the interplay of tectonic, metamorphic, and metasomatic processes. Here we present new geochronological data using Rb-Sr internal mineral isochrons and in situ Ar-40/Ar-39 laser ablation data to provide constraints on the relative importance of fluid-mediated mineral replacement reactions and diffusion for the interpretation of radiogenic isotope signatures, and on the use of these isotopic systems for dating metamorphic and variably deformed rocks. Our study focuses on the shear zone at the contact between two major lithological units of the Sesia zone, the eclogitic micaschists and the gneiss minuti. Metasedimentary rocks of the eclogitic micaschists unit contain phengite with step-like zoning in major element chemistry as evidence for petrologic disequilibrium. Distinct Ar-40/Ar-39 spot ages of relict phengite cores and over-printed rims demonstrate the preservation of individual age domains in the crystals. The eclogitic micaschists also show systematic Sr isotope disequilibria among different phengite populations, so that minimum ages of relict assemblage crystallization can be differentiated from the timing of late increments of deformation. The preservation of these disequilibrium features shows the lack of diffusive re-equilibration and underpins that fluid-assisted dissolution and recrystallization reactions are the main factors controlling the isotope record in these subduction-related metamorphic rocks. Blueschist-facies mylonites record deformation along the major shear zone that separates the eclogitic micaschists from the gneiss minuti. Two Rb-Sr isochrones that comprise several white mica fractions and glaucophane constrain the timing of this deformation and accompanying near-complete blueschist-facies re-equilibration of the Rb-Sr system to 60.1 +/- 0.9 Ma and 60.9 +/- 2.1 Ma, respectively. Overlapping ages in eclogitic micaschists of 60.1 +/- 1.1 (Rb-Sr isochron of sheared matrix assemblage), 58.6 +/- 0.8, and 60.9 +/- 0.4 Ma (white mica Ar-40/Ar-39 inverse isochron ages) support the significance of this age and show that fluid-rock interaction and partial re-equilibration occurred as much as several kilometers away from the shear zone. An earlier equilibration during high-pressure conditions in the eclogitic mica schists is recorded in minimum Rb-Sr ages for relict assemblages (77.2 +/- 0.8 and 72.4 +/- 1.1 Ma) and an Ar-40/Ar-39 inverse isochron age of 75.4 +/- 0.8 Ma for white mica cores, again demonstrating that the two isotope systems provide mutually supporting geochronological information. Local reactivation and recrystallization along the shear zone lasted >15 m.y., as late increments of deformation are recorded in a greenschist-facies mylonite by a Rb-Sr isochron age of 46.5 +/- 0.7 Ma.}, language = {en} } @misc{CheilakouTsopelasAnastasopoulosetal.2018, author = {Cheilakou, E. and Tsopelas, N. and Anastasopoulos, A. and Kourousis, D. and Rychkov, Dmitry and Gerhard, Reimund and Frankenstein, B. and Amditis, A. and Damigos, Y. and Bouklas, C.}, title = {Strain monitoring system for steel and concrete structures}, series = {Procedia Structural Integrity}, volume = {10}, journal = {Procedia Structural Integrity}, publisher = {Elsevier}, address = {Amsterdam}, issn = {2452-3216}, doi = {10.1016/j.prostr.2018.09.005}, pages = {25 -- 32}, year = {2018}, abstract = {The present work is part of a collaborative H2020 European funded research project called SENSKIN, that aims to improve Structural Health Monitoring (SHM) for transport infrastructure through the development of an innovative monitoring and management system for bridges based on a novel, inexpensive, skin-like sensor. The integrated SENSKIN technology will be implemented in the case of steel and concrete bridges, and tested, field-evaluated and benchmarked on actual bridge environment against a conventional health monitoring solution developed by Mistras Group Hellas. The main objective of the present work is to implement the autonomous, fully functional strain monitoring system based on commercially available off-the-shelf components, that will be used to accomplish direct comparison between the performance of the innovative SENSKIN sensors and the conventional strain sensors commonly used for structural monitoring of bridges. For this purpose, the mini Structural Monitoring System (mini SMS) of Physical Acoustics Corporation, a comprehensive data acquisition unit designed specifically for long-term unattended operation in outdoor environments, was selected. For the completion of the conventional system, appropriate foil-type strain sensors were selected, driven by special conditioners manufactured by Mistras Group. A comprehensive description of the strain monitoring system and its peripheral components is provided in this paper. For the evaluation of the integrated system's performance and the effect of various parameters on the long-term behavior of sensors, several test steel pieces instrumented with different strain sensors configurations were prepared and tested in both laboratory and field ambient conditions. Furthermore, loading tests were performed aiming to validate the response of the system in monitoring the strains developed in steel beam elements subject to bending regimes. Representative results obtained from the above experimental tests have been included in this paper as well.}, language = {en} } @article{WengLuedekeZempetal.2018, author = {Weng, Wei and L{\"u}deke, Matthias K. B. and Zemp, Delphine Clara and Lakes, Tobia and Kropp, J{\"u}rgen}, title = {Aerial and surface rivers}, series = {Hydrology and earth system sciences : HESS}, volume = {22}, journal = {Hydrology and earth system sciences : HESS}, number = {1}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1027-5606}, doi = {10.5194/hess-22-911-2018}, pages = {911 -- 927}, year = {2018}, abstract = {The abundant evapotranspiration provided by the Amazon forests is an important component of the hydrological cycle, both regionally and globally. Since the last century, deforestation and expanding agricultural activities have been changing the ecosystem and its provision of moisture to the atmosphere. However, it remains uncertain how the ongoing land use change will influence rainfall, runoff, and water availability as findings from previous studies differ. Using moisture tracking experiments based on observational data, we provide a spatially detailed analysis recognizing potential teleconnection between source and sink regions of atmospheric moisture. We apply land use scenarios in upwind moisture sources and quantify the corresponding rainfall and runoff changes in downwind moisture sinks. We find spatially varying responses of water regimes to land use changes, which may explain the diverse results from previous studies. Parts of the Peruvian Amazon and western Bolivia are identified as the sink areas most sensitive to land use change in the Amazon and we highlight the current water stress by Amazonian land use change on these areas in terms of the water availability. Furthermore, we also identify the influential source areas where land use change may considerably reduce a given target sink's water reception (from our example of the Ucayali River basin outlet, rainfall by 5-12 \% and runoff by 19-50 \% according to scenarios). Sensitive sinks and influential sources are therefore suggested as hotspots for achieving sustainable land-water management.}, language = {en} } @article{PrahlBoettleCostaetal.2018, author = {Prahl, Boris F. and Boettle, Markus and Costa, Lu{\´i}s F{\´i}l{\´i}pe Carvalho da and Kropp, J{\"u}rgen and Rybski, Diego}, title = {Damage and protection cost curves for coastal floods within the 600 largest European cities}, series = {Scientific Data}, volume = {5}, journal = {Scientific Data}, publisher = {Nature Publ. Group}, address = {London}, issn = {2052-4463}, doi = {10.1038/sdata.2018.34}, pages = {18}, year = {2018}, abstract = {The economic assessment of the impacts of storm surges and sea-level rise in coastal cities requires high-level information on the damage and protection costs associated with varying flood heights. We provide a systematically and consistently calculated dataset of macroscale damage and protection cost curves for the 600 largest European coastal cities opening the perspective for a wide range of applications. Offering the first comprehensive dataset to include the costs of dike protection, we provide the underpinning information to run comparative assessments of costs and benefits of coastal adaptation. Aggregate cost curves for coastal flooding at the city-level are commonly regarded as by-products of impact assessments and are generally not published as a standalone dataset. Hence, our work also aims at initiating a more critical discussion on the availability and derivation of cost curves.}, language = {en} } @article{HoferichterLaetschLazaridesetal.2018, author = {Hoferichter, Frances and Laetsch, Alexander and Lazarides, Rebecca and Raufelder, Diana}, title = {The big-fish-little-pond effect on the four facets of academic self-concept}, series = {Frontiers in psychology}, volume = {9}, journal = {Frontiers in psychology}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-1078}, doi = {10.3389/fpsyg.2018.01247}, pages = {11}, year = {2018}, abstract = {The social context plays a decisive role in the formation of the academic self-concept (ASC) and has been widely studied as the big-fish-little-pond-effect (BFLPE). This effect describes that comparable talented students in high-achieving school settings have a lower ASC compared to equally talented students attending low-achieving settings. Past research has focused on students' domain-specific ASC, while little is known about the relation of achievement-related classroom compositions and the various facets of ASC. Additionally, BFLPE-research has been critiqued to build its theoretical frame on social comparison theory, without providing sufficient empirical support. To address this gap, we analyzed how the single student's social, criterial, absolute, and individual ASC relate to class-level achievement of 8th graders. Applying Multilevel Structural Equation Modeling (MLSEM) we found that all facets of ASC were significantly related to average-class achievement, while student's social ASC revealed the strongest associated. The results reveal explicitly that average-class achievement is strongly related to social comparison processes.}, language = {en} } @article{HoangMertensWessigetal.2018, author = {Hoang, Hoa T. and Mertens, Monique and Wessig, Pablo and Sellrie, Frank and Schenk, J{\"o}rg A. and Kumke, Michael Uwe}, title = {Antibody Binding at the Liposome-Water Interface}, series = {ACS Omega}, volume = {3}, journal = {ACS Omega}, number = {12}, publisher = {American Chemical Society}, address = {Washington}, issn = {2470-1343}, doi = {10.1021/acsomega.8b03016}, pages = {18109 -- 18116}, year = {2018}, abstract = {Different signal amplification strategies to improve the detection sensitivity of immunoassays have been applied which utilize enzymatic reactions, nanomaterials, or liposomes. The latter are very attractive materials for signal amplification because liposomes can be loaded with a large amount of signaling molecules, leading to a high sensitivity. In addition, liposomes can be used as a cell-like "bioscaffold" to directly test recognition schemes aiming at cell-related processes. This study demonstrates an easy and fast approach to link the novel hydrophobic optical probe based on [1,3]dioxolo[4,5-f]-[1,3]benzodioxole (DBD dye mm239) with tunable optical properties to hydrophilic recognition elements (e.g., antibodies) using liposomes for signal amplification and as carrier of the hydrophobic dye. The fluorescence properties of mm239 (e.g., long fluorescence lifetime, large Stokes shift, high photostability, and high quantum yield), its high hydrophobicity for efficient anchoring in liposomes, and a maleimide bioreactive group were applied in a unique combination to build a concept for the coupling of antibodies or other protein markers to liposomes (coupling to membranes can be envisaged). The concept further allowed us to avoid multiple dye labeling of the antibody. Here, anti-TAMRA-antibody (DC7-Ab) was attached to the liposomes. In proof-of-concept, steady-state as well as time-resolved fluorescence measurements (e.g., fluorescence depolarization) in combination with single molecule detection (fluorescence correlation spectroscopy, FCS) were used to analyze the binding interaction between DC7-Ab and liposomes as well as the binding of the antigen rhodamine 6G (R6G) to the antibody. Here, the Forster resonance energy transfer (FRET) between mm239 and R6G was monitored. In addition to ensemble FRET data, single-molecule FRET (PIE-FRET) experiments using pulsed interleaved excitation were used to characterize in detail the binding on a single-molecule level to avoid averaging out effects.}, language = {en} } @article{KasyanenkoUnksovBakulevetal.2018, author = {Kasyanenko, Nina and Unksov, Ivan and Bakulev, Vladimir and Santer, Svetlana}, title = {DNA interaction with head-to-tail associates of cationic surfactants prevents formation of compact particles}, series = {Molecules}, volume = {23}, journal = {Molecules}, number = {7}, publisher = {MDPI}, address = {Basel}, issn = {1420-3049}, doi = {10.3390/molecules23071576}, pages = {14}, year = {2018}, abstract = {Cationic azobenzene-containing surfactants are capable of condensing DNA in solution with formation of nanosized particles that can be employed in gene delivery. The ratio of surfactant/DNA concentration and solution ionic strength determines the result of DNA-surfactant interaction: Complexes with a micelle-like surfactant associates on DNA, which induces DNA shrinkage, DNA precipitation or DNA condensation with the emergence of nanosized particles. UV and fluorescence spectroscopy, low gradient viscometry and flow birefringence methods were employed to investigate DNA-surfactant and surfactant-surfactant interaction at different NaCl concentrations, [NaCl]. It was observed that [NaCl] (or the Debye screening radius) determines the surfactant-surfactant interaction in solutions without DNA. Monomers, micelles and non-micellar associates of azobenzene-containing surfactants with head-to-tail orientation of molecules were distinguished due to the features of their absorption spectra. The novel data enabled us to conclude that exactly the type of associates (together with the concentration of components) determines the result of DNA-surfactant interaction. Predomination of head-to-tail associates at 0.01 M < [NaCl] < 0.5 M induces DNA aggregation and in some cases DNA precipitation. High NaCl concentration (higher than 0.8 M) prevents electrostatic attraction of surfactants to DNA phosphates for complex formation. DAPI dye luminescence in solutions with DNA-surfactant complexes shows that surfactant tails overlap the DNA minor groove. The addition of di- and trivalent metal ions before and after the surfactant binding to DNA indicate that the bound surfactant molecules are located on DNA in islets.}, language = {en} } @article{ReichelRheinHofmannetal.2018, author = {Reichel, Martin and Rhein, Cosima and Hofmann, Lena M. and Monti, Juliana and Japtok, Lukasz and Langgartner, Dominik and F{\"u}chsl, Andrea M. and Kleuser, Burkhard and Gulbins, Erich and Hellerbrand, Claus and Reber, Stefan O. and Kornhuber, Johannes}, title = {Chronic Psychosocial Stress in Mice Is Associated With Increased Acid Sphingomyelinase Activity in Liver and Serum and With Hepatic C16:0-Ceramide Accumulation}, series = {Frontiers in Psychiatry}, volume = {9}, journal = {Frontiers in Psychiatry}, publisher = {Frontiers Research Foundation}, address = {Lausanne}, issn = {1664-0640}, doi = {10.3389/fpsyt.2018.00496}, pages = {8}, year = {2018}, abstract = {Chronic psychosocial stress adversely affects human morbidity and is a risk factor for inflammatory disorders, liver diseases, obesity, metabolic syndrome, and major depressive disorder (MDD). In recent studies, we found an association of MDD with an increase of acid sphingomyelinase (ASM) activity. Thus, we asked whether chronic psychosocial stress as a detrimental factor contributing to the emergence of MDD would also affect ASM activity and sphingolipid (SL) metabolism. To induce chronic psychosocial stress in male mice we employed the chronic subordinate colony housing (CSC) paradigm and compared them to non-stressed single housed control (SHC) mice. We determined Asm activity in liver and serum, hepatic SL concentrations as well as hepatic mRNA expression of genes involved in SL metabolism. We found that hepatic Asm activity was increased by 28\% (P = 0.006) and secretory Asm activity by 47\% (P = 0.002) in stressed mice. C16:0-Cer was increased by 40\% (P = 0.008). Gene expression analysis further revealed an increased expression of tumor necrosis factor (TNF)-alpha (P = 0.009) and of several genes involved in SL metabolism (Cers5, P = 0.028; Cers6, P = 0.045; Gba, P = 0.049; Gba2, P = 0.030; Ormdl2, P = 0.034; Smpdl3B; P = 0.013). Our data thus provides first evidence that chronic psychosocial stress, at least in mice, induces alterations in SL metabolism, which in turn might be involved in mediating the adverse health effects of chronic psychosocial stress and peripheral changes occurring in mood disorders.}, language = {en} } @article{FarhanRudolphNoecheletal.2018, author = {Farhan, Muhammad and Rudolph, Tobias and N{\"o}chel, Ulrich and Kratz, Karl and Lendlein, Andreas}, title = {Extractable Free Polymer Chains Enhance Actuation Performance of Crystallizable Poly(epsilon-caprolactone) Networks and Enable Self-Healing}, series = {Polymers}, volume = {10}, journal = {Polymers}, number = {3}, publisher = {MDPI}, address = {Basel}, issn = {2073-4360}, doi = {10.3390/polym10030255}, pages = {15}, year = {2018}, abstract = {Crosslinking of thermoplastics is a versatile method to create crystallizable polymer networks, which are of high interest for shape-memory actuators. Here, crosslinked poly(epsilon-caprolactone) thermosets (cPCLs) were prepared from linear starting material, whereby the amount of extractable polymer was varied. Fractions of 5-60 wt \% of non-crosslinked polymer chains, which freely interpenetrate the crosslinked network, were achieved leading to differences in the resulting phase of the bulk material. This can be described as "sponge-like" with open or closed compartments depending on the amount of interpenetrating polymer. The crosslinking density and the average network chain length remained in a similar range for all network structures, while the theoretical accessible volume for reptation of the free polymer content is affected. This feature could influence or introduce new functions into the material created by thermomechanical treatment. The effect of interpenetrating PCL in cPCLs on the reversible actuation was analyzed by cyclic, uniaxial tensile tests. Here, high reversible strains of up to Delta epsilon = 24\% showed the enhanced actuation performance of networks with a non-crosslinked PCL content of 30 wt \% resulting from the crystal formation in the phase of the non-crosslinked PCL and co-crystallization with network structures. Additional functionalities are reprogrammability and self-healing capabilities for networks with high contents of extractable polymer enabling reusability and providing durable actuator materials.}, language = {en} } @article{WillnerLevermannZhaoetal.2018, author = {Willner, Sven N. and Levermann, Anders and Zhao, Fang and Frieler, Katja}, title = {Adaptation required to preserve future high-end river flood risk at present levels}, series = {Science Advances}, volume = {4}, journal = {Science Advances}, number = {1}, publisher = {American Assoc. for the Advancement of Science}, address = {Washington}, issn = {2375-2548}, doi = {10.1126/sciadv.aao1914}, pages = {8}, year = {2018}, abstract = {Earth's surface temperature will continue to rise for another 20 to 30 years even with the strongest carbon emission reduction currently considered. The associated changes in rainfall patterns can result in an increased flood risk worldwide. We compute the required increase in flood protection to keep high-end fluvial flood risk at present levels. The analysis is carried out worldwide for subnational administrative units. Most of the United States, Central Europe, and Northeast and West Africa, as well as large parts of India and Indonesia, require the strongest adaptation effort. More than half of the United States needs to at least double their protection within the next two decades. Thus, the need for adaptation to increased river flood is a global problem affecting industrialized regions as much as developing countries.}, language = {en} } @article{ChaykovskaHeunischvonEinemetal.2018, author = {Chaykovska, Lyubov and Heunisch, Fabian and von Einem, Gina and Hocher, Carl-Friedrich and Tsuprykov, Oleg and Pavkovic, Mira and Sandner, Peter and Kretschmer, Axel and Chu, Chang and Elitok, Saban and Stasch, Johannes-Peter and Hocher, Berthold}, title = {Urinary cGMP predicts major adverse renal events in patients with mild renal impairment and/or diabetes mellitus before exposure to contrast medium}, series = {PLoS one}, volume = {13}, journal = {PLoS one}, number = {4}, publisher = {PLoS}, address = {San Fransisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0195828}, pages = {13}, year = {2018}, abstract = {Background The use of iodine-based contrast agents entails the risk of contrast induced nephropathy (CIN). Radiocontrast agents elicit the third most common cause of nephropathy among hospitalized patients, accounting for 11-12\% of cases. CIN is connected with clinically significant consequences, including increased morbidity, prolonged hospitalization, increased risk of complications, potential need for dialysis, and increased mortality rate. The number of in hospital examinations using iodine-based contrast media has been significantly increasing over the last decade. In order to protect patients from possible complications of such examinations, new biomarkers are needed that are able to predict a risk of contrast-induced nephropathy. Urinary and plasma cyclic guanosine monophosphate (cGMP) concentrations are influenced by renal function. Urinary cGMP is primarily of renal cellular origin. Therefore, we assessed if urinary cGMP concentration may predict major adverse renal events (MARE) after contrast media exposure during coronary angiography. Methods Urine samples were prospectively collected from non-randomized consecutive patients with either diabetes or preexisting impaired kidney function receiving intra-arterial contrast medium (CM) for emergent or elective coronary angiography at the Charite Campus Mitte, University Hospital Berlin. Urinary cGMP concentration in spot urine was analyzed 24 hours after CM exposure. Patients were followed up over 90 days for occurrence of death, initiation of dialysis, doubling of plasma creatinine concentration or MARE. Results In total, 289 consecutive patients were included into the study. Urine cGMP/creatinine ratio 24 hours before CM exposure expressed as mean +/- SD was predictive for the need of dialysis (no dialysis: 89.77 +/- 92.85 mu M/mM, n = 277; need for dialysis: 140.3 +/- 82.90 mu M/mM, n = 12, p = 0.008), death (no death during follow-up: 90.60 +/- 92.50 mu M/mM, n = 280; death during follow-up: 169.88 +/- 81.52 mu M/mM, n = 9; p = 0.002), and the composite endpoint MARE (no MARE: 86.02 +/- 93.17 mu M/mM, n = 271; MARE: 146.64 +/- 74.68 mu M/mM, n = 18, p<0.001) during the follow-up of 90 days after contrast media application. cGMP/creatinine ratio stayed significantly increased at values exceeding 120 pM/mM in patients who developed MARE, required dialysis or died. Conclusions Urinary cGMP/creatinine ratio >= 120 mu M/mM before CM exposure is a promising biomarker for the need of dialysis and all-cause mortality 90 days after CM exposure in patients with preexisting renal impairment or diabetes.}, language = {en} }