@article{Jobst2007, author = {Jobst, Anne}, title = {"Briefe wie gemahlt"}, volume = {VIII}, number = {15}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {1617-5239}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-41654}, pages = {6 -- 9}, year = {2007}, abstract = {Am 19. Dezember 1831 wurde der verdienstvolle Mikrobiologe Christian Gottfried Ehrenberg zum korrespondierenden Mitglied des Institut de France gew{\"a}hlt. Alexander von Humboldt, der Ehrenberg als Wissenschaftler hoch sch{\"a}tzte, hat sich energisch f{\"u}r diese Wahl eingesetzt. Ein Zeugnis seines Engagements ist ein Brief an den Mediziner Antoine Baron Portal (1742-1832), den er um seine Stimme bei der bevorstehenden Wahl bat. Die Geschichte der Wahl Ehrenbergs in Verbindung mit diesem Brief und die Gr{\"u}nde f{\"u}r Humboldts Interesse daran sollen hier kurz skizziert werden.}, language = {de} } @article{Holl2012, author = {Holl, Frank}, title = {"Die zweitgr{\"o}ßte Beleidigung des Menschen sei die Sklaverei ..."}, volume = {XIII}, number = {25}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {1617-5239}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-62413}, pages = {46 -- 62}, year = {2012}, abstract = {Daniel Kehlmann gibt vor, mit seinem Roman Die Vermessung der Welt „verschwiegene oder {\"u}bersehene Wahrheiten sichtbar" zu machen. Dadurch, dass er die Leser bewusst im Zweifel l{\"a}sst, was historisch belegt und was erfunden ist, entstehen Missverst{\"a}ndnisse. Der Beitrag analysiert die wichtigsten Charakteristika des Kehlmann'schen und des historischen Alexander von Humboldt und weist nach, dass diese nicht {\"u}bereinstimmen. Der Aufsatz fragt nach Kehlmanns Rolle, der in der {\"O}ffentlichkeit gerne die Pose des Gelehrten einnimmt, seine Erfindungen jedoch nicht offenlegt und sie als angebliches Humboldt-Zitat sogar in einem wissenschaftlichen Text publiziert. Der Beitrag kommt zu dem Schluss, dass alle, die etwas f{\"u}r ihre Allgemeinbildung tun m{\"o}chten, bei Die Vermessung der Welt an der falschen Adresse sind.}, language = {de} } @article{GamezGuadixWachsWright2020, author = {Gamez-Guadix, Manuel and Wachs, Sebastian and Wright, Michelle F.}, title = {"Haters back off!" psychometric properties of the coping with cyberhate questionnaire and relationship with well-being in Spanish adolescents}, series = {Psicothema}, volume = {32}, journal = {Psicothema}, number = {4}, publisher = {Colegio oficial de psicologos de asturias}, address = {Oviedo}, issn = {0214-9915}, doi = {10.7334/psicothema2020.219}, pages = {567 -- 574}, year = {2020}, abstract = {Background: Cyberhate is a growing form of online aggression against a person or a group based on race, ethnicity, nationality, sexual orientation, gender, religion, or disability. The present study aims to examine psychometric properties of the Coping with Cyberhate Questionnaire, the prevalence of coping strategies in Spanish adolescents, differences in coping strategies based in sex, age, and victim status, and the association between coping with cyberhate and adolescents' mental well-being. Method: The sample consisted of 1,005 adolescents between 12 and 18 years old (Mage = 14.28 years, SD = 1.63; 51.9\% girls) who completed self-report measures on coping strategies, victimization status, and mental well-being. Results: The results of confirmatory factor analyses showed a structure for the Coping with Cyberhate Questionnaire composed of six factors, namely Distal advice, Assertiveness, Helplessness/Selfblame, Close support, Technical coping, and Retaliation. It demonstrated acceptable internal consistency. The three most frequently endorsed coping strategies were Technical coping, Close support, and Assertiveness. In addition, lower Helplessness/Self-blame, and higher Close-support, Assertiveness, and Distal advice were significantly related to adolescents' better mental well-being. Conclusion: Prevention programs that educate adolescents about how to deal with cyberhate are needed.}, language = {en} } @article{Capra2022, author = {Capra, Elena Sofia}, title = {"Orfeo out of Care"}, series = {thersites 15}, volume = {2022}, journal = {thersites 15}, number = {15}, editor = {Amb{\"u}hl, Annemarie and Carl{\`a}-Uhink, Filippo and Rollinger, Christian and Walde, Christine}, issn = {2364-7612}, doi = {10.34679/thersites.vol15.209}, pages = {52 -- 89}, year = {2022}, abstract = {The paper focuses on an example of multiple-step reception: the contribution of the classical story of Orpheus and Eurydice and the mediaeval lay Sir Orfeo to Tolkien's work. In the first part, I compare the lay with Virgilian and Ovidian versions of Orpheus' myth. This comparison shows the anonymous author's deep knowledge of the ancient texts and complex way of rewriting them through stealing and hybridization. The lay was highly esteemed by Tolkien, who translated it and took inspiration from it while describing the Elven kingdom in The Hobbit and building the storyline of Beren and L{\´u}thien in The Silmarillion. Through this key tale, Orpheus/Orfeo's romance has a deep influence also on Aragorn and Arwen's story in The Lord of the Rings. The most important element that Tolkien takes from the Sir Orfeo figuration of the ancient story is undoubtedly the insertion of political theme: the link established between the recovery of the main character's beloved and the return to royal responsability. The second part of the paper is, thus, dedicated to the reception of Sir Orfeo and the classical myth in Tolkien. It shows how in his work the different steps of the tradition of Orpheus' story are co-present, creating an inextricable substrate of inspiration that nourishes his imagination.}, language = {en} } @article{MassonBambergStrickeretal.2019, author = {Masson, Torsten and Bamberg, Sebastian and Stricker, Michael and Heidenreich, Anna}, title = {"We can help ourselves": does community resilience buffer against the negative impact of flooding on mental health?}, series = {Natural hazards and earth system sciences}, volume = {19}, journal = {Natural hazards and earth system sciences}, number = {11}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {1561-8633}, doi = {10.5194/nhess-19-2371-2019}, pages = {2371 -- 2384}, year = {2019}, abstract = {Empirical evidence of the relationship between social support and post-disaster mental health provides support for a general beneficial effect of social support (main-effect model; Wheaton, 1985). From a theoretical perspective, a buffering effect of social support on the negative relationship between disaster-related stress and mental health also seems plausible (stress-buffering model; Wheaton, 1985). Previous studies, however, (a) have paid less attention to the buffering effect of social support and (b) have mainly relied on interpersonal support (but not collective-level support such as community resilience) when investigating this issue. This previous work might have underestimated the effect of support on post-disaster mental health. Building on a sample of residents in Germany recently affected by flooding (N = 118), we show that community resilience to flooding (but not general interpersonal social support) buffered against the negative effects of flooding on post-disaster mental health. The results support the stress-buffering model and call for a more detailed look at the relationship between support and resilience and post-disaster adjustment, including collective-level variables.}, language = {en} } @incollection{WetzelReedSchenck2023, author = {Wetzel, Johanna M. and Reed, Kate and Schenck, Marcia C.}, title = {"Writing with my professors"}, series = {Writing Together: Kollaboratives Schreiben mit Personen aus dem Feld}, volume = {45}, booktitle = {Writing Together: Kollaboratives Schreiben mit Personen aus dem Feld}, publisher = {Transcript Verlag}, address = {Bielefeld}, isbn = {978-3-8394-6399-4}, doi = {10.14361/9783839463994-002}, pages = {31 -- 53}, year = {2023}, abstract = {Kollaboratives Forschen quer zu hegemonialen Wissensordnungen gilt als wichtiger Baustein dekolonialer Wissenspraxis. Gemeinsame Schreibprozesse von Wissenschaftler*innen und ihren nicht-wissenschaftlichen Forschungspartner*innen sind allerdings selten und eine methodologische und forschungspraktische Reflexion fehlt. Die Beitr{\"a}ger*innen widmen sich diesen L{\"u}cken, indem sie erfolgreiche, aber auch gescheiterte Projekte kollaborativer Textproduktion zwischen Universit{\"a}t und Feld vorstellen und auf ihr Potenzial als transformative und dekoloniale Wissenspraxis befragen. So entsteht eine praktische Orientierungshilfe, die gleichzeitig die interdisziplin{\"a}re Diskussion anregt.}, language = {en} } @misc{SalzerNoebauer2021, author = {Salzer, Leonhard and N{\"o}bauer, Anna}, title = {(Auf) Humboldts Spuren}, series = {HiN : Alexander von Humboldt im Netz}, volume = {XXII}, journal = {HiN : Alexander von Humboldt im Netz}, number = {43}, editor = {Ette, Ottmar and Knobloch, Eberhard}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {2568-3543}, doi = {10.25932/publishup-53327}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-533271}, pages = {65 -- 82}, year = {2021}, abstract = {Vor seiner Besteigung des Antisana in Ecuador verbrachte Alexander von Humboldt mit seinem Expeditionsteam die Nacht vom 15. auf den 16. M{\"a}rz 1802 in einer Hacienda am Fuße des Vulkangipfels, deren letztes bauliches Zeugnis eine steinerne H{\"u}tte darstellt. Bauforscherische Untersuchungen eines internationalen Forscherteams konnten die mehrschichtige Bau- und Reparaturgeschichte dieses Baudenkmals ermitteln und {\"u}ber eine Auswertung von Reiseberichten mehrerer Andenforscher die Nutzungsgeschichte des einzelnen Geb{\"a}udes und des gesamten Anwesens kl{\"a}ren. Schließlich ergaben sich daraus neue Erkenntnisse zu Humboldts Aufenthalt am Antisana.}, language = {de} } @article{Pezzini2022, author = {Pezzini, Giuseppe}, title = {(Classical) Narratives of Decline in Tolkien: Renewal, Accommodation, Focalisation}, series = {thersites 15}, volume = {2022}, journal = {thersites 15}, number = {15}, editor = {Amb{\"u}hl, Annemarie and Carl{\`a}-Uhink, Filippo and Rollinger, Christian and Walde, Christine}, issn = {2364-7612}, doi = {10.34679/thersites.vol15.213}, pages = {25 -- 51}, year = {2022}, abstract = {The paper investigates Tolkien's narratives of decline through the lens of their classical ancestry. Narratives of decline are widespread in ancient culture, in both philosophical and literary discourses. They normally posit a gradual degradation (moral and ontological) from an idealized Golden Age, which went hand-in-hand with increasing detachment of gods from mortal affairs. Narratives of decline are also at the core of Tolkien's mythology, constituting yet another underresearched aspect of classical influence on Tolkien. Such Classical narratives reverberate e.g. in Tolkien's division of Arda's history into ages, from an idealized First Age filled with Joy and Light to a Third Age, described as "Twilight Age (…) the first of the broken and changed world" (Letters 131). More generally, these narratives are related to Tolkien's notorious perception of history as a "long defeat" (Letters 195) and to that "heart-racking sense of the vanished past" which pervades Tolkien's works - the emotion which, in his words, moved him "supremely" and which he found "small difficulty in evoking" (Letters 91). The paper analyses the reception of narratives of decline in Tolkien's legendarium, pointing out similarities but also contrasts and differences, with the aim to discuss some key patterns of (classical) reception in Tolkien's theory and practice ('renewal', 'accommodation', 'focalization').}, language = {en} } @article{FrohnLiebschPech2023, author = {Frohn, Julia and Liebsch, Ann-Catherine and Pech, Detlef}, title = {(Meta-)Reflexion f{\"u}r Inklusion}, series = {Reflexion in der Lehrkr{\"a}ftebildung: Empirisch - Phasen{\"u}bergreifend - Interdisziplin{\"a}r (Potsdamer Beitr{\"a}ge zur Lehrerbildung und Bildungsforschung ; 4)}, journal = {Reflexion in der Lehrkr{\"a}ftebildung: Empirisch - Phasen{\"u}bergreifend - Interdisziplin{\"a}r (Potsdamer Beitr{\"a}ge zur Lehrerbildung und Bildungsforschung ; 4)}, number = {4}, editor = {Mientus, Lukas and Klempin, Christiane and Nowak, Anna}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-566-8}, issn = {2626-3556}, doi = {10.25932/publishup-62910}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-629103}, pages = {139 -- 146}, year = {2023}, abstract = {Im Sinne einer „Meta-Reflexivit{\"a}t" zielt dieser Beitrag darauf ab, den strukturtheoretischen und kompetenzorientierten Professionalisierungsansatz im Konstrukt der adaptiven Lehrkompetenz zusammenzuf{\"u}hren, was vor allem f{\"u}r inklusionsorientierte Ans{\"a}tze vielversprechend erscheint: Anhand der Konstruktfacetten adaptiver diagnostischer, didaktischer sowie Sach- und Klassenf{\"u}hrungskompetenz werden m{\"o}gliche Herangehensweisen f{\"u}r eine inklusionsorientierte Lehrkr{\"a}ftebildung formuliert, die sowohl konkrete Kompetenzbereiche benennen als auch die Reflexion entsprechender Spannungsverh{\"a}ltnisse im strukturtheoretischen Sinne voraussetzen. So soll der Beitrag einen knappen theoretischen Aufriss zur Zusammenf{\"u}hrung der unterschiedlichen Professionalisierungsans{\"a}tze unter der Pr{\"a}misse (mehr) Reflexion f{\"u}r (mehr) Inklusion leisten.}, language = {de} } @article{Haenel2023, author = {H{\"a}nel, Hilkje C.}, title = {(Moralisch) guter Sex}, series = {Zeitschrift f{\"u}r Praktische Philosophie}, volume = {9}, journal = {Zeitschrift f{\"u}r Praktische Philosophie}, number = {2}, publisher = {Universit{\"a}t Salzburg}, address = {Salzburg}, issn = {2409-9961}, doi = {10.22613/zfpp/9.2.2}, pages = {49 -- 78}, year = {2023}, abstract = {In einem k{\"u}rzlich erschienenen Artikel argumentiert Almut v. Wedelstaedt {\"u}berzeugend, warum Zustimmung zwar „die Bedingung f{\"u}r die Legitimation von Sex" ist (2020, 127), dass die moralische G{\"u}te von Sex aber nur dann einzusch{\"a}tzen ist, wenn wir darauf achten, ob die Beteiligten der Handlung sich auf Augenh{\"o}he begegnen. Die Idee ist: Es gibt legitime sexuelle Handlungen, die moralisch gut sind, und es gibt legitime sexuelle Handlungen, die moralisch besser sind. Hier m{\"o}chte ich die Idee des besseren Sexes genauer ausloten. W{\"a}hrend v. Wedelstaedt von moralisch gelungenem Sex spricht und somit auf der Ebene der moralischen Bewertung von Sex bleibt, m{\"o}chte ich die Frage danach stellen, was Sex qualitativ gut macht. Tats{\"a}chlich wird in der Zustimmungsdebatte meist davon ausgegangen, dass diese zwei Fragen wenig gemeinsam haben; ob eine sexuelle Handlung legitim ist, hat zun{\"a}chst nichts damit zu tun, ob diese auch gut ist. Ich werde drei Argumente liefern, warum wir legitimen Sex und qualitativ guten Sex zusammen betrachten sollten - und es wird sich zeigen, dass die gegenw{\"a}rtige philosophische und rechtstheoretische Debatte Zustimmung verk{\"u}rzt diskutiert und daher alleingenommen wenig hilfreich ist, stattdessen ben{\"o}tigt die Zustimmungsdebatte auch eine Untersuchung von qualitativ gutem Sex.}, language = {de} } @article{ZimmermannDeVeaughGeissToennisetal.2020, author = {Zimmermann, Malte and De Veaugh-Geiss, Joseph P. and T{\"o}nnis, Swantje and Onea, Edgar}, title = {(Non-)exhaustivity in focus partitioning across languages}, series = {Approaches to Hungarian}, volume = {16}, journal = {Approaches to Hungarian}, publisher = {John Benjamins}, address = {Amsterdam}, pages = {24}, year = {2020}, abstract = {We present novel experimental evidence on the availability and the status of exhaustivity inferences with focus partitioning in German, English, and Hungarian. Results suggest that German and English focus-background clefts and Hungarian focus share important properties, ({\´E}. Kiss 1998, 1999; Szabolcsi 1994; Percus 1997; Onea \& Beaver 2009). Those constructions are anaphoric devices triggering an existence presupposition. EXH-inferences are not obligatory in such constructions in English, German, or Hungarian, against some previous literature (Percus 1997; B{\"u}ring \& Križ 2013; {\´E}. Kiss 1998), but in line with pragmatic analyses of EXH-inferences in clefts (Horn 1981, 2016; Pollard \& Yasavul 2016). The cross-linguistic differences in the distribution of EXH-inferences are attributed to properties of the Hungarian number marking system.}, language = {en} } @article{SchulzeBettBivouretal.2020, author = {Schulze, Patricia S. C. and Bett, Alexander J. and Bivour, Martin and Caprioglio, Pietro and Gerspacher, Fabian M. and Kabakl{\i}, {\"O}zde Ş. and Richter, Armin and Stolterfoht, Martin and Zhang, Qinxin and Neher, Dieter and Hermle, Martin and Hillebrecht, Harald and Glunz, Stefan W. and Goldschmidt, Jan Christoph}, title = {25.1\% high-efficiency monolithic perovskite silicon tandem solar cell with a high bandgap perovskite absorber}, series = {Solar RRL}, volume = {4}, journal = {Solar RRL}, number = {7}, publisher = {John Wiley \& Sons, Inc.}, address = {New Jersey}, pages = {10}, year = {2020}, abstract = {Monolithic perovskite silicon tandem solar cells can overcome the theoretical efficiency limit of silicon solar cells. This requires an optimum bandgap, high quantum efficiency, and high stability of the perovskite. Herein, a silicon heterojunction bottom cell is combined with a perovskite top cell, with an optimum bandgap of 1.68 eV in planar p-i-n tandem configuration. A methylammonium-free FA(0.75)Cs(0.25)Pb(I0.8Br0.2)(3) perovskite with high Cs content is investigated for improved stability. A 10\% molarity increase to 1.1 m of the perovskite precursor solution results in approximate to 75 nm thicker absorber layers and 0.7 mA cm(-2) higher short-circuit current density. With the optimized absorber, tandem devices reach a high fill factor of 80\% and up to 25.1\% certified efficiency. The unencapsulated tandem device shows an efficiency improvement of 2.3\% (absolute) over 5 months, showing the robustness of the absorber against degradation. Moreover, a photoluminescence quantum yield analysis reveals that with adapted charge transport materials and surface passivation, along with improved antireflection measures, the high bandgap perovskite absorber has the potential for 30\% tandem efficiency in the near future.}, language = {en} } @article{BoldrighiniFrigioMaponietal.2020, author = {Boldrighini, Carlo and Frigio, Sandro and Maponi, Pierluigi and Pellegrinotti, Alessandro and Sinai, Yakov G.}, title = {3-D incompressible Navier-Stokes equations: Complex blow-up and related real flows}, series = {Lectures in pure and applied mathematics}, journal = {Lectures in pure and applied mathematics}, number = {6}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-485-2}, issn = {2199-4951}, doi = {10.25932/publishup-47220}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-472201}, pages = {185 -- 194}, year = {2020}, language = {en} } @article{FranzSudoKhomenko2022, author = {Franz, Gerhard and Sudo, Masafumi and Khomenko, Vladimir}, title = {40Ar/39Ar dating of a hydrothermal pegmatitic buddingtonite-muscovite assemblage from Volyn, Ukraine}, series = {European journal of mineralogy : EJM : an international journal on mineralogy, petrology, geochemistry, and related sciences}, volume = {34}, journal = {European journal of mineralogy : EJM : an international journal on mineralogy, petrology, geochemistry, and related sciences}, number = {1}, publisher = {Copernicus}, address = {G{\"o}ttingen}, issn = {0935-1221}, doi = {10.5194/ejm-34-7-2022}, pages = {7 -- 18}, year = {2022}, abstract = {We determined Ar-40/Ar-39 ages of buddingtonite, occurring together with muscovite, with the laser-ablation method. This is the first attempt to date the NH4-feldspar buddingtonite, which is typical for sedimentary-diagenetic environments of sediments, rich in organic matter, or in hydrothermal environments, associated with volcanic geyser systems. The sample is a hydrothermal breccia, coming from the Paleoproterozoic pegmatite field of the Korosten Plutonic Complex, Volyn, Ukraine. A detailed characterization by optical methods, electron microprobe analyses, backscattered electron imaging, and IR analyses showed that the buddingtonite consists of euhedral-appearing platy crystals of tens of micrometers wide, 100 or more micrometers in length, which consist of fine-grained fibers of <= 1 mu m thickness. The crystals are sector and growth zoned in terms of K-NH4-H3O content. The content of K allows for an age determination with the Ar-40/Ar-39 method, as well as in the accompanying muscovite, intimately intergrown with the buddingtonite. The determinations on muscovite yielded an age of 1491 +/- 9 Ma, interpreted as the hydrothermal event forming the breccia. However, buddingtonite apparent ages yielded a range of 563 +/- 14 Ma down to 383 +/- 12 Ma, which are interpreted as reset ages due to Ar loss of the fibrous buddingtonite crystals during later heating. We conclude that buddingtonite is suited for Ar-40/Ar-39 age determinations as a supplementary method, together with other methods and minerals; however, it requires a detailed mineralogical characterization, and the ages will likely represent minimum ages.}, language = {en} } @article{KrmičekTimmermanZiemannetal.2020, author = {Krm{\´i}ček, Luk{\´a}š and Timmerman, Martin Jan and Ziemann, Martin Andreas and Sudo, Masafumi and Ulrych, Jaromir}, title = {40Ar/39Ar step-heating dating of phlogopite and kaersutite megacrysts from the Železn{\´a} hůrka (Eisenb{\"u}hl) Pleistocene scoria cone, Czech Republic}, series = {Geologica Carpathica}, volume = {71}, journal = {Geologica Carpathica}, number = {4}, publisher = {Veda}, address = {Bratislava}, issn = {1335-0552}, doi = {10.31577/GeolCarp.71.4.6}, pages = {382 -- 387}, year = {2020}, abstract = {(40)A/Ar-39 step-heating of mica and amphibole megacrysts from hauyne-bearing olivine melilitite scoria/tephra from the Zelezna hurka yielded a 435 +/- 108 ka isotope correlation age for phlogopite and a more imprecise 1.55 Ma total gas age of the kaersutite megacryst. The amphibole megacrysts may constitute the first, and the younger phlogopite megacrysts the later phase of mafic, hydrous melilitic magma crystallization. It cannot be ruled out that the amphibole megacrysts are petrogenetically unrelated to tephra and phlogopite megacrysts and were derived from mantle xenoliths or disaggregated older, deep crustal pegmatites. This is in line both with the rarity of amphibole at Zelezna hurka and with the observed signs of magmatic resorption at the edges of amphibole crystals.}, language = {en} } @article{ChristakoudiTsilidisMulleretal.2020, author = {Christakoudi, Sofa and Tsilidis, Konstantinos K. and Muller, David C. and Freisling, Heinz and Weiderpass, Elisabete and Overvad, Kim and S{\"o}derberg, Stefan and H{\"a}ggstr{\"o}m, Christel and Pischon, Tobias and Dahm, Christina C. and Zhang, Jie and Tj{\o}nneland, Anne and Schulze, Matthias Bernd}, title = {A Body Shape Index (ABSI) achieves better mortality risk stratification than alternative indices of abdominal obesity: results from a large European cohort}, series = {Scientific Reports}, volume = {10}, journal = {Scientific Reports}, number = {1}, publisher = {Springer Nature}, address = {Berlin}, pages = {15}, year = {2020}, abstract = {Abdominal and general adiposity are independently associated with mortality, but there is no consensus on how best to assess abdominal adiposity. We compared the ability of alternative waist indices to complement body mass index (BMI) when assessing all-cause mortality. We used data from 352,985 participants in the European Prospective Investigation into Cancer and Nutrition (EPIC) and Cox proportional hazards models adjusted for other risk factors. During a mean follow-up of 16.1 years, 38,178 participants died. Combining in one model BMI and a strongly correlated waist index altered the association patterns with mortality, to a predominantly negative association for BMI and a stronger positive association for the waist index, while combining BMI with the uncorrelated A Body Shape Index (ABSI) preserved the association patterns. Sex-specific cohort-wide quartiles of waist indices correlated with BMI could not separate high-risk from low-risk individuals within underweight (BMI<18.5 kg/m(2)) or obese (BMI30 kg/m(2)) categories, while the highest quartile of ABSI separated 18-39\% of the individuals within each BMI category, which had 22-55\% higher risk of death. In conclusion, only a waist index independent of BMI by design, such as ABSI, complements BMI and enables efficient risk stratification, which could facilitate personalisation of screening, treatment and monitoring.}, language = {en} } @article{HaueisStechKubick2022, author = {Haueis, Lisa and Stech, Marlitt and Kubick, Stefan}, title = {A Cell-free Expression Pipeline for the Generation and Functional Characterization of Nanobodies}, series = {Frontiers in Bioengineering and Biotechnology}, volume = {10}, journal = {Frontiers in Bioengineering and Biotechnology}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2296-4185}, doi = {10.3389/fbioe.2022.896763}, pages = {11}, year = {2022}, abstract = {Cell-free systems are well-established platforms for the rapid synthesis, screening, engineering and modification of all kinds of recombinant proteins ranging from membrane proteins to soluble proteins, enzymes and even toxins. Also within the antibody field the cell-free technology has gained considerable attention with respect to the clinical research pipeline including antibody discovery and production. Besides the classical full-length monoclonal antibodies (mAbs), so-called "nanobodies" (Nbs) have come into focus. A Nb is the smallest naturally-derived functional antibody fragment known and represents the variable domain (VHH, similar to 15 kDa) of a camelid heavy-chain-only antibody (HCAb). Based on their nanoscale and their special structure, Nbs display striking advantages concerning their production, but also their characteristics as binders, such as high stability, diversity, improved tissue penetration and reaching of cavity-like epitopes. The classical way to produce Nbs depends on the use of living cells as production host. Though cell-based production is well-established, it is still time-consuming, laborious and hardly amenable for high-throughput applications. Here, we present for the first time to our knowledge the synthesis of functional Nbs in a standardized mammalian cell-free system based on Chinese hamster ovary (CHO) cell lysates. Cell-free reactions were shown to be time-efficient and easy-to-handle allowing for the "on demand" synthesis of Nbs. Taken together, we complement available methods and demonstrate a promising new system for Nb selection and validation.}, language = {en} } @article{KrebsRakotoarinoroStechetal.2022, author = {Krebs, Simon K. and Rakotoarinoro, Nathanael and Stech, Marlitt and Zemella, Anne and Kubick, Stefan}, title = {A CHO-based cell-free dual fluorescence reporter system for the straightforward assessment of amber suppression and scFv functionality}, series = {Frontiers in Bioengineering and Biotechnology}, volume = {10}, journal = {Frontiers in Bioengineering and Biotechnology}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2296-4185}, doi = {10.3389/fbioe.2022.873906}, pages = {15}, year = {2022}, abstract = {Incorporation of noncanonical amino acids (ncAAs) with bioorthogonal reactive groups by amber suppression allows the generation of synthetic proteins with desired novel properties. Such modified molecules are in high demand for basic research and therapeutic applications such as cancer treatment and in vivo imaging. The positioning of the ncAA-responsive codon within the protein's coding sequence is critical in order to maintain protein function, achieve high yields of ncAA-containing protein, and allow effective conjugation. Cell-free ncAA incorporation is of particular interest due to the open nature of cell-free systems and their concurrent ease of manipulation. In this study, we report a straightforward workflow to inquire ncAA positions in regard to incorporation efficiency and protein functionality in a Chinese hamster ovary (CHO) cell-free system. As a model, the well-established orthogonal translation components Escherichia coli tyrosyl-tRNA synthetase (TyrRS) and tRNATyr(CUA) were used to site-specifically incorporate the ncAA p-azido-l-phenylalanine (AzF) in response to UAG codons. A total of seven ncAA sites within an anti-epidermal growth factor receptor (EGFR) single-chain variable fragment (scFv) N-terminally fused to the red fluorescent protein mRFP1 and C-terminally fused to the green fluorescent protein sfGFP were investigated for ncAA incorporation efficiency and impact on antigen binding. The characterized cell-free dual fluorescence reporter system allows screening for ncAA incorporation sites with high incorporation efficiency that maintain protein activity. It is parallelizable, scalable, and easy to operate. We propose that the established CHO-based cell-free dual fluorescence reporter system can be of particular interest for the development of antibody-drug conjugates (ADCs).}, language = {en} } @article{BaumgartBoosBraunsmann2023, author = {Baumgart, Lene and Boos, Pauline and Braunsmann, Katharina}, title = {A circulatory loop}, series = {Social inclusion}, volume = {11}, journal = {Social inclusion}, number = {4}, publisher = {Cogitatio Press}, address = {Lisbon}, issn = {2183-2803}, doi = {10.17645/si.v11i4.7056}, pages = {160 -- 171}, year = {2023}, abstract = {In the digitalization debate, gender biases in digital technologies play a significant role because of their potential for social exclusion and inequality. It is therefore remarkable that organizations as drivers of digitalization and as places for social integration have been widely overlooked so far. Simultaneously, gender biases and digitalization have structurally immanent connections to organizations. Therefore, a look at the reciprocal relationship between organizations, digitalization, and gender is needed. The article provides answers to the question of whether and how organizations (re)produce, reinforce, or diminish gender-specific inequalities during their digital transformations. On the one hand, gender inequalities emerge when organizations use post-bureaucratic concepts through digitalization. On the other hand, gender inequalities are reproduced when organizations either program or implement digital technologies and fail to establish control structures that prevent gender biases. This article shows that digitalization can act as a catalyst for inequality-producing mechanisms, but also has the potential to mitigate inequalities. We argue that organizations must be considered when discussing the potential of exclusion through digitalization.}, language = {en} } @article{MbebiBreitlerBordeauxetal.2022, author = {Mbebi, Alain J. and Breitler, Jean-Christophe and Bordeaux, M'elanie and Sulpice, Ronan and McHale, Marcus and Tong, Hao and Toniutti, Lucile and Castillo, Jonny Alonso and Bertrand, Benoit and Nikoloski, Zoran}, title = {A comparative analysis of genomic and phenomic predictions of growth-related traits in 3-way coffee hybrids}, series = {G3: Genes, genomes, genetics}, volume = {12}, journal = {G3: Genes, genomes, genetics}, number = {9}, publisher = {Genetics Soc. of America}, address = {Pittsburgh, PA}, issn = {2160-1836}, doi = {10.1093/g3journal/jkac170}, pages = {11}, year = {2022}, abstract = {Genomic prediction has revolutionized crop breeding despite remaining issues of transferability of models to unseen environmental conditions and environments. Usage of endophenotypes rather than genomic markers leads to the possibility of building phenomic prediction models that can account, in part, for this challenge. Here, we compare and contrast genomic prediction and phenomic prediction models for 3 growth-related traits, namely, leaf count, tree height, and trunk diameter, from 2 coffee 3-way hybrid populations exposed to a series of treatment-inducing environmental conditions. The models are based on 7 different statistical methods built with genomic markers and ChlF data used as predictors. This comparative analysis demonstrates that the best-performing phenomic prediction models show higher predictability than the best genomic prediction models for the considered traits and environments in the vast majority of comparisons within 3-way hybrid populations. In addition, we show that phenomic prediction models are transferrable between conditions but to a lower extent between populations and we conclude that chlorophyll a fluorescence data can serve as alternative predictors in statistical models of coffee hybrid performance. Future directions will explore their combination with other endophenotypes to further improve the prediction of growth-related traits for crops.}, language = {en} }