@article{SchefflerHermanussen2023,
  author    = {Scheffler, Christiane and Hermanussen, Michael},
  title     = {What does stunting tell us?},
  series = {Human biology and public health},
  volume    = {2022},
  journal   = {Human biology and public health},
  number    = {3},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  issn      = {2748-9957},
  doi       = {10.52905/hbph2022.3.36},
  pages     = {1 -- 15},
  year      = {2023},
  abstract  = {Stunting is commonly linked with undernutrition. Yet, already after World War I, German pediatricians questioned this link and stated that no association exists between nutrition and height. Recent analyses within different populations of Low- and middle-income countries with high rates of stunted children failed to support the assumption that stunted children have a low BMI and skinfold sickness as signs of severe caloric deficiency. So, stunting is not a synonym of malnutrition. Parental education level has a positive influence on body height in stunted populations, e.g., in India and in Indonesia. Socially disadvantaged children tend to be shorter and lighter than children from affluent families. Humans are social mammals; they regulate growth similar to other social mammals. Also in humans, body height is strongly associated with the position within the social hierarchy, reflecting the personal and group-specific social, economic, political, and emotional environment. These non-nutritional impact factors on growth are summarized by the concept of SEPE (Social-Economic-Political-Emotional) factors. SEPE reflects on prestige, dominance-subordination, social identity, and ego motivation of individuals and social groups.},
  language  = {en}
}
@article{SchefflerNguyenHermanussen2021,
  author    = {Scheffler, Christiane and Nguyen, Thi Hong and Hermanussen, Michael},
  title     = {Vietnamese migrants are as tall as they want to be},
  series = {Human biology and public health},
  journal   = {Human biology and public health},
  number    = {2},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  issn      = {2748-9957},
  doi       = {10.52905/hbph.v2.12},
  year      = {2021},
  abstract  = {Background: Members of the same social group tent to have the same body height. Migrants tend to adjust in height to their host communities. Objectives: Social-Economic-Political-Emotional (SEPE) factors influence growth. We hypothesized that Vietnamese young adult migrants in Germany (1) are taller than their parents, (2) are as tall as their German peers, and (3) are as tall as predicted by height expectation at age 13 years. Sample: The study was conducted in 30 male and 54 female Vietnamese migrants (mean age 26.23 years. SD=4.96) in Germany in 2020. Methods: Information on age, sex, body height, school and education, job, height and ethnicity of best friend, migration history and cultural identification, parental height and education, and recalled information on their personal height expectations at age 13 years were obtained by questionnaire. The data were analyzed by St. Nicolas House Analysis (SNHA) and multiple regression. Results: Vietnamese young adults are taller than their parents (females 3.85cm, males 7.44cm), but do not fully attain height of their German peers. The body height is positively associated with the height of best friend (p < 0.001), the height expectation at age 13 year (p < 0.001), and father's height (p=0.001). Conclusion: Body height of Vietnamese migrants in Germany reflects competitive growth and strategic growth adjustments. The magnitude of this intergenerational trend supports the concept that human growth depends on SEPE factors.},
  language  = {en}
}
@article{KiemelGurkeParaskevopoulouetal.2022,
  author    = {Kiemel, Katrin and Gurke, Marie and Paraskevopoulou, Sofia and Havenstein, Katja and Weithoff, Guntram and Tiedemann, Ralph},
  title     = {Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species},
  series = {Scientific Reports},
  volume    = {12},
  journal   = {Scientific Reports},
  publisher = {Springer Nature},
  address   = {London},
  issn      = {2045-2322},
  doi       = {10.1038/s41598-022-27137-3},
  pages     = {14},
  year      = {2022},
  abstract  = {Genetic divergence and the frequency of hybridization are central for defining species delimitations, especially among cryptic species where morphological differences are merely absent. Rotifers are known for their high cryptic diversity and therefore are ideal model organisms to investigate such patterns. Here, we used the recently resolved Brachionus calyciflorus species complex to investigate whether previously observed between species differences in thermotolerance and gene expression are also reflected in their genomic footprint. We identified a Heat Shock Protein gene (HSP 40 kDa) which exhibits cross species pronounced sequence variation. This gene exhibits species-specific fixed sites, alleles, and sites putatively under positive selection. These sites are located in protein binding regions involved in chaperoning and may therefore reflect adaptive diversification. By comparing three genetic markers (ITS, COI, HSP 40 kDa), we revealed hybridization events between the cryptic species. The low frequency of introgressive haplotypes/alleles suggest a tight, but not fully impermeable boundary between the cryptic species.},
  language  = {en}
}
@article{JonesGonzalezFortesConnelletal.2015,
  author    = {Jones, Eppie R. and Gonz{\´a}lez-Fortes, Gloria M. and Connell, Sarah and Siska, Veronika and Eriksson, Anders and Martiniano, Rui and McLaughlin, Russell L. and Llorente, Marcos Gallego and Cassidy, Lara M. and Gamba, Cristina and Meshveliani, Tengiz and Bar-Yosef, Ofer and Mueller, Werner and Belfer-Cohen, Anna and Matskevich, Zinovi and Jakeli, Nino and Higham, Thomas F. G. and Currat, Mathias and Lordkipanidze, David and Hofreiter, Michael and Manica, Andrea and Pinhasi, Ron and Bradley, Daniel G.},
  title     = {Upper Palaeolithic genomes reveal deep roots of modern Eurasians},
  series = {Nature Communications},
  volume    = {6},
  journal   = {Nature Communications},
  publisher = {Nature Publishing Group},
  address   = {London},
  issn      = {2041-1723},
  doi       = {10.1038/ncomms9912},
  pages     = {8},
  year      = {2015},
  abstract  = {We extend the scope of European palaeogenomics by sequencing the genomes of Late Upper Palaeolithic (13,300 years old, 1.4-fold coverage) and Mesolithic (9,700 years old, 15.4-fold) males from western Georgia in the Caucasus and a Late Upper Palaeolithic (13,700 years old, 9.5-fold) male from Switzerland. While we detect Late Palaeolithic-Mesolithic genomic continuity in both regions, we find that Caucasus hunter-gatherers (CHG) belong to a distinct ancient clade that split from western hunter-gatherers similar to 45 kya, shortly after the expansion of anatomically modern humans into Europe and from the ancestors of Neolithic farmers similar to 25 kya, around the Last Glacial Maximum. CHG genomes significantly contributed to the Yamnaya steppe herders who migrated into Europe similar to 3,000 BC, supporting a formative Caucasus influence on this important Early Bronze age culture. CHG left their imprint on modern populations from the Caucasus and also central and south Asia possibly marking the arrival of Indo-Aryan languages.},
  language  = {en}
}
@article{BormkiKnoescheSchneider2003,
  author    = {Bormki, G. and Kn{\"o}sche, Ralf and Schneider, Ingo},
  title     = {Untersuchungen zur N-Mineralisation und -Remobilisierung im Sediment eines extrem flachen Auensees (Schollener See)},
  series = {Brandenburgische Umwelt-Berichte : BUB ; Schriftenreihe der Mathematisch-Naturwissenschaftlichen Fakult{\"a}t der Universit{\"a}t Potsdam},
  volume    = {13},
  journal   = {Brandenburgische Umwelt-Berichte : BUB ; Schriftenreihe der Mathematisch-Naturwissenschaftlichen Fakult{\"a}t der Universit{\"a}t Potsdam},
  issn      = {1434-2375},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-4081},
  pages     = {114 -- 120},
  year      = {2003},
  language  = {de}
}
@phdthesis{Senger2007,
  author    = {Senger, Toralf},
  title     = {Untersuchungen zur Metallhom{\"o}ostase in Arabidopsis thaliana},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-13234},
  school      = {Universit{\"a}t Potsdam},
  year      = {2007},
  abstract  = {Alle Organismen sind f{\"u}r ihr {\"U}berleben auf Metalle angewiesen. Hierbei gibt es f{\"u}r jedes Metall einen Konzentrationsbereich, der das Optimum zwischen Metallmangel, -bedarf und -toxizit{\"a}t darstellt. Es gilt mittlerweile als erwiesen, dass alle Organismen zur Aufrechterhaltung des Metallgleichgewichts ein komplexes Netzwerk von Proteinen und niedermolekularen Verbindungen entwickelt haben. Die molekularen Komponenten dieses Netzwerks sind nur zu einem Teil bekannt und charakterisiert: In den letzten Jahren wurden einige Proteinfamilien identifiziert, deren Mitglieder Metalle durch Lipidmembranen transportieren. Eine dieser Metalltransporterfamilien ist die Cation Diffusion Facilitator (CDF)-Familie: Alle charakterisierten Mitglieder exportieren Metalle aus dem Zytoplasma - entweder in zellul{\"a}re Kompartimente oder aus der Zelle heraus. Von den zw{\"o}lf Mitgliedern dieser Familie in Arabidopsis thaliana (A. thaliana) - Metall Toleranz Protein (MTP)-1 bis -12 - wurden bisher AtMTP1 und AtMTP3 charakterisiert. In dieser Arbeit wird die Charakterisierung von AtMTP2 beschrieben. Wie die homologen Proteine AtMTP1 und AtMTP3 f{\"u}hrt AtMTP2 zu Zn-Toleranz, wenn es heterolog in Zn-sensitiven Hefemutanten exprimiert wird. Mit AtMTP2 transformierte Hefemutanten zeigten dar{\"u}ber hinaus erh{\"o}hte Co-Toleranz. Expression von chim{\"a}ren AtMTP2/GFP Fusionsproteinen in Hefe, A.thaliana protoplasten und in stabil transformierten A.thalinana Planzenlinien deutet auf Lokalisation of AtMTP2 in Membranen des Endoplasmatischen Retikulums (ER) hin, wenn GFP an den C-Terminus von MTP2 fusioniert wird. Fusion of GFP an den N-Terminus von AtMTP2 f{\"u}hrte zu Lokalisation in der vakuol{\"a}ren Membran, was wahrscheinlichsten auf Fehllokalisierung durch Maskierung eines ER-Retentionsmotivs (XXRR) am N-Terminus von AtMTP2 zur{\"u}ckgeht. Dies legt nahe, dass AtMTP2 die erw{\"a}hnten Metalle in das Endomembransystem der Zelle transportieren kann. Eine gewebespezifische Lokalisierung wurde mit Pflanzen durchgef{\"u}hrt, die das β-Glucuronidase (GUS)-Reporterprotein bzw. chim{\"a}re Fusionsproteine aus EGFP und AtMTP2 unter Kontrolle des nativen pMTP2-Promotors exprimierten. Diese Experimente best{\"a}tigten zum einen, dass der pMTP2-Promotor nur unter Zn-Defizienz aktiv ist. GUS-Aktivit{\"a}t wurde unter diesen Bedingungen in zwei Zonen der Wurzelspitze beobachtet: in den isodiametrischen Zellen der meristematischen Zone und in der beginnenden Wurzelhaarzone. Dar{\"u}ber hinaus konnte gezeigt werden, dass die EGFP-Fusionsproteine unter Kontrolle des nativen pMTP2-Promotors nur in epidermalen Zellen exprimiert werden. F{\"u}r eine homozygote Knockout- Linie, mtp2-S3, konnte bisher kein eindeutiger Ph{\"a}notyp identifiziert werden. Auf Grundlage der bisher durchgef{\"u}hrten Charakterisierung von AtMTP2 erscheinen zwei Modelle der Funktion von AtMTP2 in der Pflanze m{\"o}glich: AtMTP2 k{\"o}nnte essentiell f{\"u}r die Versorgung des ER mit Zn unter Zn-Mangelbedingungen sein. Hierf{\"u}r spricht, dass AtMTP2 in jungen, teilungsaktiven und damit Zn-ben{\"o}tigenden Wurzelzonen exprimiert wird. Die auf die Epidermis beschr{\"a}nkte Lokalisation k{\"o}nnte bei diesem Modell auf die M{\"o}glichkeit der zwischenzellul{\"a}ren Zn-Verteilung innerhalb des ER {\"u}ber Desmotubules hindeuten. Alternativ k{\"o}nnte AtMTP2 eine Funktion bei der Detoxifizierung von Zn unter Zn-Schock Bedingungen haben: Es ist bekannt, dass unter Zn- Mangelbedingungen die Expression der zellul{\"a}ren Zn-Aufnahmesysteme hochreguliert wird. Wenn nun die Zn-Verf{\"u}gbarkeit im Boden z. B durch eine pH-{\"A}nderung innerhalb kurzer Zeit stark ansteigt, besteht die Notwendigkeit der Entgiftung von Zn innerhalb der Zelle, bis der starke Einstrom von Zn ins Zytoplasma durch die Deaktivierung der Zn-Aufnahmesysteme und einer geringeren Expression in der Pflanze gedrosselt ist. Ein {\"a}hnlicher Mechanismus wurde in der B{\"a}ckerhefe S. cerevisae beschrieben, in der dar{\"u}ber hinaus ein Zn-Transporter verst{\"a}rkt exprimiert wird, der Zn durch Transport in die Vakuole entgiften kann. Es ist durchaus m{\"o}glich, dass in Arabidopsis AtMTP2 die Zn-Detoxifizierung unter diesen speziellen Bedingungen durch Zn-Transport in das ER oder die Vakuole vermittelt. Zur Identifikation weiterer Komponenten des Metallhom{\"o}ostasenetzwerks sind verschiedene Ans{\"a}tze denkbar. In dieser Arbeit wurde in Hefe ein heterologer Screen durchgef{\"u}hrt, um Interaktoren f{\"u}r vier Mitglieder der Arabidopsis-CDF-Familie zu identifizieren. Unter den 11 im Hefesystem best{\"a}tigten Kandidaten befindet sich mit AtSPL1 ein AtMTP1-Interaktionskandidat, der m{\"o}glicherweise eine Rolle bei der Cu-,Zn-Hom{\"o}ostase spielt. Als wahrscheinliche AtMTP3-Interaktionskandidaten wurde die c"-Untereinheit der vakuol{\"a}ren H+-ATPase AtVHA identifiziert sowie mit AtNPSN13 ein Protein, das vermutlich eine Rolle bei Fusionen von Vesikeln mit Zielmembranen spielt. Ein anderer Ansatz zur Identifikation neuer Metallhom{\"o}ostasegene ist die vergleichende Elementanalyse von nat{\"u}rlichen oder mutagenisierten Pflanzenpopulationen. Voraussetzung f{\"u}r diesen Ansatz ist die schnelle und genaue Analyse des Elementgehalts von Pflanzen. Eine etablierte Methode zur simultanen Bestimmung von bis zu 65 Elementen in einer Probe ist die Inductively Coupled Plasma Optical Emission Spectrometry (ICP OES). Der limitierende Faktor f{\"u}r einen hohen Probendurchsatz ist die Notwendigkeit, Proben f{\"u}r die Analyse zu verfl{\"u}ssigen. Eine alternative Methode der Probenzuf{\"u}hrung zum Analyseger{\"a}t ist die elektrothermale Verdampfung (ETV) der Probe. Zur weitgehend automatisierten Analyse von Pflanzenmaterial mit minimiertem Arbeitsaufwand wurde eine Methode entwickelt, die auf der Kopplung der ETV mit der ICP OES basiert.},
  language  = {de}
}
@article{EckertHerdenStiftetal.2022,
  author    = {Eckert, Silvia and Herden, Jasmin and Stift, Marc and Durka, Walter and Kleunen, Mark van and Joshi, Jasmin},
  title     = {Traces of genetic but not epigenetic adaptation in the invasive goldenrod Solidago canadensis despite the absence of population structure},
  series = {Frontiers in Ecology and Evolution},
  volume    = {10},
  journal   = {Frontiers in Ecology and Evolution},
  publisher = {Frontiers},
  address   = {Lausanne, Schweiz},
  issn      = {2296-701X},
  doi       = {10.3389/fevo.2022.856453},
  pages     = {1 -- 17},
  year      = {2022},
  abstract  = {Biological invasions may result from multiple introductions, which might compensate for reduced gene pools caused by bottleneck events, but could also dilute adaptive processes. A previous common-garden experiment showed heritable latitudinal clines in fitness-related traits in the invasive goldenrod Solidago canadensis in Central Europe. These latitudinal clines remained stable even in plants chemically treated with zebularine to reduce epigenetic variation. However, despite the heritability of traits investigated, genetic isolation-by-distance was non-significant. Utilizing the same specimens, we applied a molecular analysis of (epi)genetic differentiation with standard and methylation-sensitive (MSAP) AFLPs. We tested whether this variation was spatially structured among populations and whether zebularine had altered epigenetic variation. Additionally, we used genome scans to mine for putative outlier loci susceptible to selection processes in the invaded range. Despite the absence of isolation-by-distance, we found spatial genetic neighborhoods among populations and two AFLP clusters differentiating northern and southern Solidago populations. Genetic and epigenetic diversity were significantly correlated, but not linked to phenotypic variation. Hence, no spatial epigenetic patterns were detected along the latitudinal gradient sampled. Applying genome-scan approaches (BAYESCAN, BAYESCENV, RDA, and LFMM), we found 51 genetic and epigenetic loci putatively responding to selection. One of these genetic loci was significantly more frequent in populations at the northern range. Also, one epigenetic locus was more frequent in populations in the southern range, but this pattern was lost under zebularine treatment. Our results point to some genetic, but not epigenetic adaptation processes along a large-scale latitudinal gradient of S. canadensis in its invasive range.},
  language  = {en}
}
@article{TiedemannIobbiNivolLeimkuehler2022,
  author    = {Tiedemann, Kim and Iobbi-Nivol, Chantal and Leimk{\"u}hler, Silke},
  title     = {The Role of the Nucleotides in the Insertion of the bis-Molybdopterin Guanine Dinucleotide Cofactor into apo-Molybdoenzymes},
  series = {Molecules},
  volume    = {27},
  journal   = {Molecules},
  edition   = {9},
  publisher = {MDPI},
  address   = {Basel, Schweiz},
  issn      = {1420-3049},
  doi       = {10.3390/molecules27092993},
  pages     = {1 -- 15},
  year      = {2022},
  abstract  = {The role of the GMP nucleotides of the bis-molybdopterin guanine dinucleotide (bis-MGD) cofactor of the DMSO reductase family has long been a subject of discussion. The recent characterization of the bis-molybdopterin (bis-Mo-MPT) cofactor present in the E. coli YdhV protein, which differs from bis-MGD solely by the absence of the nucleotides, now enables studying the role of the nucleotides of bis-MGD and bis-MPT cofactors in Moco insertion and the activity of molybdoenzymes in direct comparison. Using the well-known E. coli TMAO reductase TorA as a model enzyme for cofactor insertion, we were able to show that the GMP nucleotides of bis-MGD are crucial for the insertion of the bis-MGD cofactor into apo-TorA.},
  language  = {en}
}
@article{OgunkolaGuiraudieCaprazFeronetal.2023,
  author    = {Ogunkola, Moses Olalekan and Guiraudie-Capraz, Gaelle and F{\´e}ron, Fran{\c{c}}ois and Leimk{\"u}hler, Silke},
  title     = {The Human Mercaptopyruvate Sulfurtransferase TUM1 Is Involved in Moco Biosynthesis, Cytosolic tRNA Thiolation and Cellular Bioenergetics in Human Embryonic Kidney Cells},
  series = {Biomolecules},
  volume    = {13},
  journal   = {Biomolecules},
  edition   = {1},
  publisher = {MDPI},
  address   = {Basel, Schweiz},
  issn      = {2218-273X},
  doi       = {10.3390/biom13010144},
  pages     = {1 -- 23},
  year      = {2023},
  abstract  = {Sulfur is an important element that is incorporated into many biomolecules in humans. The incorporation and transfer of sulfur into biomolecules is, however, facilitated by a series of different sulfurtransferases. Among these sulfurtransferases is the human mercaptopyruvate sulfurtransferase (MPST) also designated as tRNA thiouridine modification protein (TUM1). The role of the human TUM1 protein has been suggested in a wide range of physiological processes in the cell among which are but not limited to involvement in Molybdenum cofactor (Moco) biosynthesis, cytosolic tRNA thiolation and generation of H2S as signaling molecule both in mitochondria and the cytosol. Previous interaction studies showed that TUM1 interacts with the L-cysteine desulfurase NFS1 and the Molybdenum cofactor biosynthesis protein 3 (MOCS3). Here, we show the roles of TUM1 in human cells using CRISPR/Cas9 genetically modified Human Embryonic Kidney cells. Here, we show that TUM1 is involved in the sulfur transfer for Molybdenum cofactor synthesis and tRNA thiomodification by spectrophotometric measurement of the activity of sulfite oxidase and liquid chromatography quantification of the level of sulfur-modified tRNA. Further, we show that TUM1 has a role in hydrogen sulfide production and cellular bioenergetics.},
  language  = {en}
}
@article{MummHermanussen2022,
  author    = {Mumm, Rebekka and Hermanussen, Michael},
  title     = {The dilemma of misclassifying weight in short and in historic population},
  series = {Human biology and public health},
  volume    = {2021},
  journal   = {Human biology and public health},
  number    = {3, Summer School Supplement},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  issn      = {2748-9957},
  doi       = {10.52905/hbph2021.3.28},
  pages     = {7},
  year      = {2022},
  abstract  = {Background: Clinicians often refer anthropometric measures of a child to so-called "growth standards" and "growth references. Over 140 countries have meanwhile adopted WHO growth standards. Objectives: The present study was conducted to thoroughly examine the idea of growth standards as a common yardstick for all populations. Weight depends on height. We became interested in whether also weight-for-height depends on height. First, we studied the age-group effect on weight-for-height. Thereafter, we tested the applicability of weight-for-height references in short and in historic populations. Sample and Methods: We analyzed body height and body weight and weight-for-height of 3795 healthy boys and 3726 healthy girls aged 2 to 5 years measured in East-Germany between 1986 and 1990. We chose contemporary height and weight charts from Germany, the UK, and the WHO growth chart and compared these with three geographically commensurable growth charts from the end of the 19th century. Results: We analyzed body height and body weight and weight-for-height of 3795 healthy boys and 3726 healthy girls aged 2 to 5 years measured in East-Germany between 1986 and 1990. We chose contemporary height and weight charts from Germany, the UK, and the WHO growth chart and compared these with three geographically commensurable growth charts of the end of the 19th century. Conclusion: Weight-for-height depends on age and sex and apart from the nutritional state, reflects body proportion and body built particularly during infancy and early childhood. Populations with a relatively short average height are prone to high values of weight-for-height for arithmetic reasons independent of the nutritional state.},
  language  = {en}
}
@article{SchefflerHermanussen2021,
  author    = {Scheffler, Christiane and Hermanussen, Michael},
  title     = {Stunting does not impair physical fitness in Indonesian school children},
  series = {Human biology and public health},
  journal   = {Human biology and public health},
  number    = {2},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  issn      = {2748-9957},
  doi       = {10.52905/hbph.v2.19},
  year      = {2021},
  abstract  = {Background: Physical fitness is decreased in malnourished children and adults. Poor appearance and muscular flaccidity are among the first signs of malnutrition. Malnutrition is often associated with stunting. Objectives: We test the hypotheses that stunted children of low social strata are physically less fit than children of high social strata. Sample: We investigated 354 school girls and 369 school boys aged 5.83 to 13.83 (mean 9.54) years from three different social strata in Kupang (West-Timor, Indonesia) in 2020. Methods: We measured height, weight, and elbow breadth, calculated standard deviation (SDS) of height and weight according to CDC references, and the Frame index as an indicator of long-term physical fitness, and we tested physical fitness in standing long jump and hand grip strength. Results: Children of low social strata are physically fittest. They jump longer distances, and they have higher values in the Frame index. No association exists between height SDS and physical fitness, neither in respect to standing long jump, nor to hand grip strength. Conclusion: Stunting does not impair physical fitness in Indonesian school children. Our results support the concept that SEPE (social-economic-political-emotional) factors are involved in the regulation of human growth.},
  language  = {en}
}
@misc{WackervonElert2002,
  author    = {Wacker, Alexander and von Elert, Eric},
  title     = {Strong influences of larval diet history on subsequent post-settlement growth in the freshwater mollusc Dreissena polymorpha},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-17627},
  year      = {2002},
  abstract  = {Significant seasonal variation in size at settlement has been observed in newly settled larvae of Dreissena polymorpha in Lake Constance. Diet quality, which varies temporally and spatially in freshwater habitats, has been suggested as a significant factor influencing life history and development of freshwater invertebrates. Accordingly, experiments were conducted with field-collected larvae to test the hypothesis that diet quality can determine planktonic larval growth rates, size at settlement and subsequent post-metamorphic growth rates. Larvae were fed one of two diets or starved. One diet was composed of cyanobacterial cells which are deficient in polyunsaturated fatty acids (PUFAs), and the other was a mixed diet rich in PUFAs. Freshly metamorphosed animals from the starvation treatment had a carbon content per individual 70\% lower than that of larvae fed the mixed diet. This apparent exhaustion of larval internal reserves resulted in a 50\% reduction of the postmetamorphic growth rates. Growth was also reduced in animals previously fed the cyanobacterial diet. Hence, low food quantity or low food quality during the larval stage of D. polymorpha lead to irreversible effects for postmetamorphic animals, and is related to inferior competitive abilities.},
  language  = {en}
}
@article{BornhorstSeyfried2021,
  author    = {Bornhorst, Dorothee and Seyfried, Salim},
  title     = {Strong as a hippo's heart},
  series = {Frontiers in cell and developmental biology},
  volume    = {9},
  journal   = {Frontiers in cell and developmental biology},
  publisher = {Frontiers Media},
  address   = {Lausanne, Schweiz},
  issn      = {2296-634X},
  doi       = {10.3389/fcell.2021.731101},
  pages     = {1 -- 10},
  year      = {2021},
  abstract  = {The heart is comprised of multiple tissues that contribute to its physiological functions. During development, the growth of myocardium and endocardium is coupled and morphogenetic processes within these separate tissue layers are integrated. Here, we discuss the roles of mechanosensitive Hippo signaling in growth and morphogenesis of the zebrafish heart. Hippo signaling is involved in defining numbers of cardiac progenitor cells derived from the secondary heart field, in restricting the growth of the epicardium, and in guiding trabeculation and outflow tract formation. Recent work also shows that myocardial chamber dimensions serve as a blueprint for Hippo signaling-dependent growth of the endocardium. Evidently, Hippo pathway components act at the crossroads of various signaling pathways involved in embryonic zebrafish heart development. Elucidating how biomechanical Hippo signaling guides heart morphogenesis has direct implications for our understanding of cardiac physiology and pathophysiology.},
  language  = {en}
}
@article{LiuZhouFettke2021,
  author    = {Liu, Qingting and Zhou, Yuan and Fettke, J{\"o}rg},
  title     = {Starch granule size and morphology of Arabidopsis thaliana starch-related mutants analyzed during diurnal rhythm and development},
  series = {Molecules : a journal of synthetic chemistry and natural product chemistry / Molecular Diversity Preservation International},
  volume    = {26},
  journal   = {Molecules : a journal of synthetic chemistry and natural product chemistry / Molecular Diversity Preservation International},
  edition   = {19},
  publisher = {MDPI},
  address   = {Basel, Schweiz},
  issn      = {1420-3049},
  doi       = {10.3390/molecules26195859},
  pages     = {1 -- 9},
  year      = {2021},
  abstract  = {Transitory starch plays a central role in the life cycle of plants. Many aspects of this important metabolism remain unknown; however, starch granules provide insight into this persistent metabolic process. Therefore, monitoring alterations in starch granules with high temporal resolution provides one significant avenue to improve understanding. Here, a previously established method that combines LCSM and safranin-O staining for in vivo imaging of transitory starch granules in leaves of Arabidopsis thaliana was employed to demonstrate, for the first time, the alterations in starch granule size and morphology that occur both throughout the day and during leaf aging. Several starch-related mutants were included, which revealed differences among the generated granules. In ptst2 and sex1-8, the starch granules in old leaves were much larger than those in young leaves; however, the typical flattened discoid morphology was maintained. In ss4 and dpe2/phs1/ss4, the morphology of starch granules in young leaves was altered, with a more rounded shape observed. With leaf development, the starch granules became spherical exclusively in dpe2/phs1/ss4. Thus, the presented data provide new insights to contribute to the understanding of starch granule morphogenesis.},
  language  = {en}
}
@article{ZurellKoenigMalchowetal.2022,
  author    = {Zurell, Damaris and K{\"o}nig, Christian and Malchow, Anne-Kathleen and Kapitza, Simon and Bocedi, Greta and Travis, Justin M. J. and Fandos, Guillermo},
  title     = {Spatially explicit models for decision-making in animal conservation and restoration},
  series = {Ecography : pattern and diversity in ecology / Nordic Ecologic Society Oikos},
  journal   = {Ecography : pattern and diversity in ecology / Nordic Ecologic Society Oikos},
  number    = {4},
  publisher = {Wiley-Blackwell},
  address   = {Oxford},
  issn      = {1600-0587},
  doi       = {10.1111/ecog.05787},
  pages     = {1 -- 16},
  year      = {2022},
  abstract  = {Models are useful tools for understanding and predicting ecological patterns and processes. Under ongoing climate and biodiversity change, they can greatly facilitate decision-making in conservation and restoration and help designing adequate management strategies for an uncertain future. Here, we review the use of spatially explicit models for decision support and to identify key gaps in current modelling in conservation and restoration. Of 650 reviewed publications, 217 publications had a clear management application and were included in our quantitative analyses. Overall, modelling studies were biased towards static models (79\%), towards the species and population level (80\%) and towards conservation (rather than restoration) applications (71\%). Correlative niche models were the most widely used model type. Dynamic models as well as the gene-to-individual level and the community-to-ecosystem level were underrepresented, and explicit cost optimisation approaches were only used in 10\% of the studies. We present a new model typology for selecting models for animal conservation and restoration, characterising model types according to organisational levels, biological processes of interest and desired management applications. This typology will help to more closely link models to management goals. Additionally, future efforts need to overcome important challenges related to data integration, model integration and decision-making. We conclude with five key recommendations, suggesting that wider usage of spatially explicit models for decision support can be achieved by 1) developing a toolbox with multiple, easier-to-use methods, 2) improving calibration and validation of dynamic modelling approaches and 3) developing best-practise guidelines for applying these models. Further, more robust decision-making can be achieved by 4) combining multiple modelling approaches to assess uncertainty, and 5) placing models at the core of adaptive management. These efforts must be accompanied by long-term funding for modelling and monitoring, and improved communication between research and practise to ensure optimal conservation and restoration outcomes.},
  language  = {en}
}
@article{AgnePreickStraubeetal.2022,
  author    = {Agne, Stefanie and Preick, Michaela and Straube, Nicolas and Hofreiter, Michael},
  title     = {Simultaneous Barcode Sequencing of Diverse Museum Collection Specimens Using a Mixed RNA Bait Set},
  series = {Frontiers in Ecology and Evolution},
  volume    = {10},
  journal   = {Frontiers in Ecology and Evolution},
  publisher = {Frontiers Media S.A.},
  address   = {Lausanne, Schweiz},
  issn      = {2296-701X},
  doi       = {10.3389/fevo.2022.909846},
  pages     = {5},
  year      = {2022},
  abstract  = {A growing number of publications presenting results from sequencing natural history collection specimens reflect the importance of DNA sequence information from such samples. Ancient DNA extraction and library preparation methods in combination with target gene capture are a way of unlocking archival DNA, including from formalin-fixed wet-collection material. Here we report on an experiment, in which we used an RNA bait set containing baits from a wide taxonomic range of species for DNA hybridisation capture of nuclear and mitochondrial targets for analysing natural history collection specimens. The bait set used consists of 2,492 mitochondrial and 530 nuclear RNA baits and comprises specific barcode loci of diverse animal groups including both invertebrates and vertebrates. The baits allowed to capture DNA sequence information of target barcode loci from 84\% of the 37 samples tested, with nuclear markers being captured more frequently and consensus sequences of these being more complete compared to mitochondrial markers. Samples from dry material had a higher rate of success than wet-collection specimens, although target sequence information could be captured from 50\% of formalin-fixed samples. Our study illustrates how efforts to obtain barcode sequence information from natural history collection specimens may be combined and are a way of implementing barcoding inventories of scientific collection material.},
  language  = {en}
}
@misc{KoechyBrakenhielm2008,
  author    = {K{\"o}chy, Martin and Br{\aa}kenhielm, Sven},
  title     = {Separation of effects of moderate N deposition from natural change in ground vegetation of forests and bogs},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-16621},
  year      = {2008},
  abstract  = {The effect of moderate rates of nitrogen deposition on ground floor vegetation is poorly predicted by uncontrolled surveys or fertilization experiments using high rates of nitrogen (N) addition. We compared the temporal trends of ground floor vegetation in permanent plots with moderate (7-13 kg ha-1 year-1) and lower bulk N deposition (4-6 kg ha-1 year-1) in southern Sweden during 1982-1998. We examined whether trends differed between growth forms (vascular plants and bryophytes) and vegetation types (three types of coniferous forest, deciduous forest, and bog). Trends of site-standardized cover and richness varied among growth forms, vegetation types, and deposition regions. Cover in spruce forests decreased at the same rate with both moderate and low deposition. In pine forests cover decreased faster with moderate deposition and in bogs cover decreased faster with low deposition. Cover of bryophytes in spruce forests increased at the same rate with both moderate and low deposition. In pine forests cover decreased faster with moderate deposition and in bogs and deciduous forests there was a strong non-linear increase with moderate deposition. The trend of number of vascular plants was constant with moderate and decreased with low deposition. We found no trend in the number of bryophyte species. We propose that the decrease of cover and number with low deposition was related to normal ecosystem development (increased shading), suggesting that N deposition maintained or increased the competitiveness of some species in the moderate-deposition region. Deposition had no consistent negative effect on vegetation suggesting that it is less important than normal successional processes.},
  language  = {en}
}
@inproceedings{RossmanithBlaumKeiletal.2006,
  author    = {Rossmanith, Eva and Blaum, Niels and Keil, Manfred and Langerwisch, F. and Meyer, Jork and Popp, Alexander and Schmidt, Michael and Schultz, Christoph and Schwager, Monika and Vogel, Melanie and Wasiolka, Bernd and Jeltsch, Florian},
  title     = {Scaling up local population dynamics to regional scales},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-7320},
  year      = {2006},
  abstract  = {In semi-arid savannas, unsustainable land use can lead to degradation of entire landscapes, e.g. in the form of shrub encroachment. This leads to habitat loss and is assumed to reduce species diversity. In BIOTA phase 1, we investigated the effects of land use on population dynamics on farm scale. In phase 2 we scale up to consider the whole regional landscape consisting of a diverse mosaic of farms with different historic and present land use intensities. This mosaic creates a heterogeneous, dynamic pattern of structural diversity at a large spatial scale. Understanding how the region-wide dynamic land use pattern affects the abundance of animal and plant species requires the integration of processes on large as well as on small spatial scales. In our multidisciplinary approach, we integrate information from remote sensing, genetic and ecological field studies as well as small scale process models in a dynamic region-wide simulation tool. <hr> Interdisziplin{\"a}res Zentrum f{\"u}r Musterdynamik und Angewandte Fernerkundung Workshop vom 9. - 10. Februar 2006.},
  language  = {en}
}
@article{SchloerHirschbergBenAmoretal.2022,
  author    = {Schl{\"o}r, Anja and Hirschberg, Stefan and Ben Amor, Ghada and Meister, Toni Luise and Arora, Prerna and P{\"o}hlmann, Stefan and Hoffmann, Markus and Pf{\"a}nder, Stephanie and Eddin, Omar Kamal and Kamhieh-Milz, Julian and Hanack, Katja},
  title     = {SARS-CoV-2 neutralizing camelid heavy-chain-only antibodies as powerful tools for diagnostic and therapeutic applications},
  series = {Frontiers in Immunology},
  journal   = {Frontiers in Immunology},
  publisher = {Frontiers Media SA},
  address   = {Lausanne, Schweiz},
  issn      = {1664-3224},
  doi       = {10.3389/fimmu.2022.930975},
  pages     = {1 -- 14},
  year      = {2022},
  abstract  = {Introduction: The ongoing COVID-19 pandemic situation caused by SARS-CoV-2 and variants of concern such as B.1.617.2 (Delta) and recently, B.1.1.529 (Omicron) is posing multiple challenges to humanity. The rapid evolution of the virus requires adaptation of diagnostic and therapeutic applications. Objectives: In this study, we describe camelid heavy-chain-only antibodies (hcAb) as useful tools for novel in vitro diagnostic assays and for therapeutic applications due to their neutralizing capacity. Methods: Five antibody candidates were selected out of a na{\"i}ve camelid library by phage display and expressed as full length IgG2 antibodies. The antibodies were characterized by Western blot, enzyme-linked immunosorbent assays, surface plasmon resonance with regard to their specificity to the recombinant SARS-CoV-2 Spike protein and to SARS-CoV-2 virus-like particles. Neutralization assays were performed with authentic SARS-CoV-2 and pseudotyped viruses (wildtype and Omicron). Results: All antibodies efficiently detect recombinant SARS-CoV-2 Spike protein and SARS-CoV-2 virus-like particles in different ELISA setups. The best combination was shown with hcAb B10 as catcher antibody and HRP-conjugated hcAb A7.2 as the detection antibody. Further, four out of five antibodies potently neutralized authentic wildtype SARS-CoV-2 and particles pseudotyped with the SARS-CoV-2 Spike proteins of the wildtype and Omicron variant, sublineage BA.1 at concentrations between 0.1 and 0.35 ng/mL (ND50). Conclusion: Collectively, we report novel camelid hcAbs suitable for diagnostics and potential therapy.},
  language  = {en}
}
@misc{KoechyMathajJeltschetal.2008,
  author    = {K{\"o}chy, Martin and Mathaj, Martin and Jeltsch, Florian and Malkinson, Dan},
  title     = {Resilience of stocking capacity to changing climate in arid to Mediterranean landscapes},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-18720},
  year      = {2008},
  abstract  = {Small livestock is an important resource for rural human populations in dry climates. How strongly will climate change affect the capacity of the rangeland? We used hierarchical modelling to scale quantitatively the growth of shrubs and annual plants, the main food of sheep and goats, to the landscape extent in the eastern Mediterranean region. Without grazing, productivity increased in a sigmoid way with mean annual precipitation. Grazing reduced productivity more strongly the drier the landscape. At a point just under the stocking capacity of the vegetation, productivity declined precipitously with more intense grazing due to a lack of seed production of annuals. We repeated simulations with precipitation patterns projected by two contrasting IPCC scenarios. Compared to results based on historic patterns, productivity and stocking capacity did not differ in most cases. Thus, grazing intensity remains the stronger impact on landscape productivity in this dry region even in the future.},
  language  = {en}
}