@phdthesis{Zhang2019,
  author    = {Zhang, Shuhao},
  title     = {Synthesis and self-assembly of protein-polymer conjugates for the preparation of biocatalytically active membranes},
  school      = {Universit{\"a}t Potsdam},
  pages     = {VIII, 161},
  year      = {2019},
  abstract  = {This thesis covers the synthesis of conjugates of 2-Deoxy-D-ribose-5-phosphate aldolase (DERA) with suitable polymers and the subsequent immobilization of these conjugates in thin films via two different approaches. 2-Deoxy-D-ribose-5-phosphate aldolase (DERA) is a biocatalyst that is capable of converting acetaldehyde and a second aldehyde as acceptor into enantiomerically pure mono- and diyhydroxyaldehydes, which are important structural motifs in a number of pharmaceutically active compounds. Conjugation and immobilization renders the enzyme applicable for utilization in a continuously run biocatalytic process which avoids the common problem of product inhibition. Within this thesis, conjugates of DERA and poly(N-isopropylacrylamide) (PNIPAm) for immobilization via a self-assembly approach were synthesized and isolated, as well as conjugates with poly(N,N-dimethylacrylamide) (PDMAA) for a simplified and scalable spray-coating approach. For the DERA/PNIPAm-conjugates different synthesis routes were tested, including grafting-from and grafting-to, both being common methods for the conjugation. Furthermore, both lysines and cysteines were addressed for the conjugation in order to find optimum conjugation conditions. It turned out that conjugation via lysine causes severe activity loss as one lysine plays a key role in the catalyzing mechanism. The conjugation via the cysteines by a grafting-to approach using pyridyl disulfide (PDS) end-group functionalized polymers led to high conjugation efficiencies in the presence of polymer solubilizing NaSCN. The resulting conjugates maintained enzymatic activity and also gained high acetaldehyde tolerance which is necessary for their use later on in an industrial relevant process after their immobilization. The resulting DERA/PNIPAm conjugates exhibited enhanced interfacial activity at the air/water interface compared to the single components, which is an important pre-requisite for the immobilization via the self-assembly approach. Conjugates with longer polymer chains formed homogeneous films on silicon wafers and glass slides while the ones with short chains could only form isolated aggregates. On top of that, long chain conjugates showed better activity maintenance upon the immobilization. The crosslinking of conjugates, as well as their fixation on the support materials, are important for the mechanical stability of the films obtained from the self-assembly process. Therefore, in a second step, we introduced the UV-crosslinkable monomer DMMIBA to the PNIPAm polymers to be used for conjugation. The introduction of DMMIBA reduced the lower critical solution temperature (LCST) of the polymer and thus the water solubility at ambient conditions, resulting in lower conjugation efficiencies and in turn slightly poorer acetaldehyde tolerance of the resulting conjugates. Unlike the DERA/PNIPAm, the conjugates from the copolymer P(NIPAM-co-DMMIBA) formed continuous, homogenous films only after the crosslinking step via UV-treatment. For a firm binding of the crosslinked films, a functionalization protocol for the model support material cyclic olefin copolymer (COC) and the final target support, PAN based membranes, was developed that introduces analogue UV-reactive groups to the support surface. The conjugates immobilized on the modified COC films maintained enzymatic activity and showed good mechanical stability after several cycles of activity assessment. Conjugates with longer polymer chains, however, showed a higher degree of crosslinking after the UV-treatment leading to a pronounced loss of activity. A porous PAN membrane onto which the conjugates were immobilized as well, was finally transferred to a dead end filtration membrane module to catalyze the aldol reaction of the industrially relevant mixture of acetaldehyde and hexanal in a continuous mode. Mono aldol product was detectable, but yields were comparably low and the operational stability needs to be further improved Another approach towards immobilization of DERA conjugates that was followed, was to generate the conjugates in situ by simply mixing enzyme and polymer and spray coat the mixture onto the membrane support. Compared to the previous approach, the focus was more put on simplicity and a possible scalability of the immobilization. Conjugates were thus only generated in-situ and not further isolated and characterized. For the conjugation, PDMAA equipped with N-2-thiolactone acrylamide (TlaAm) side chains was used, an amine-reactive comonomer that can react with the lysine residues of DERA, as well as with amino groups introduced to a desired support surface. Furthermore disulfide formation after hydrolysis of the Tla groups causes a crosslinking effect. The synthesized copolymer poly(N,N-Dimethylacrylamide-co-N-2-thiolactone acrylamide) (P(DMAA-co-TlaAm)) thus serves a multiple purpose including protein binding, crosslinking and binding to support materials. The mixture of DERA and polymer could be immobilized on the PAN support by spray-coating under partial maintenance of enzymatic activity. To improve the acetaldehyde tolerance, the polymer in used was further equipped with cysteine reactive PDS end-groups that had been used for the conjugation as described in the first part of the thesis. The generated conjugates indeed showed good acetaldehyde tolerance and were thus used to be coated onto PAN membrane supports. Post treatment with a basic aqueous solution of H2O2 was supposed to further crosslink the spray-coated film hydrolysis and oxidation of the thiolactone groups. However, a washing off of the material was observed. Optimization is thus still necessary.},
  language  = {en}
}
@phdthesis{Zhou2022,
  author    = {Zhou, Shuo},
  title     = {Biological evaluation and sulfation of polymer networks from glycerol glycidyl ether},
  school      = {Universit{\"a}t Potsdam},
  pages     = {96},
  year      = {2022},
  abstract  = {Cardiovascular diseases are the main cause of death worldwide, and their prevalence is expected to rise in the coming years. Polymer-based artificial replacements have been widely used for the treatment of cardiovascular diseases. Coagulation and thrombus formation on the interfaces between the materials and the human physiological environment are key issues leading to the failure of the medical device in clinical implantation. The surface properties of the materials have a strong influence on the protein adsorption and can direct the blood cell adhesion behavior on the interfaces. Furthermore, implant-associated infections will be induced by bacterial adhesion and subsequent biofilm formation at the implantation site. Thus, it is important to improve the hemocompatibility of an implant by altering the surface properties. One of the effective strategies is surface passivation to achieve protein/cell repelling ability to reduce the risk of thrombosis. This thesis consists of synthesis, functionalization, sterilization, and biological evaluation of bulk poly(glycerol glycidyl ether) (polyGGE), which is a highly crosslinked polyether-based polymer synthesized by cationic ring-opening polymerization. PolyGGE is hypothesized to be able to resist plasma protein adsorption and bacterial adhesion due to analogous chemical structure as polyethylene glycol and hyperbranched polyglycerol. Hydroxyl end groups of polyGGE provide possibilities to be functionalized with sulfates to mimic the anti-thrombogenic function of the endothelial glycocalyx. PolyGGE was synthesized by polymerization of the commercially available monomer glycerol glycidyl ether, which was characterized as a mixture of mono-, di- and tri-glycidyl ether. Cationic ring opening-polymerization of this monomer was carried out by ultraviolet (UV) initiation of the photo-initiator diphenyliodonium hexafluorophosphate. With the increased UV curing time, more epoxides in the side chains of the monomers participated in chemical crosslinking, resulting in an increase of Young's modulus, while the value of elongation at break of polyGGE first increased due to the propagation of the polymer chains then decreased with the increase of crosslinking density. Eventually, the chain propagation can be effectively terminated by potassium hydroxide aqueous solution. PolyGGE exhibited different tensile properties in hydrated conditions at body temperature compared to the values in the dry state at room temperature. Both Young's modulus and values of elongation at break were remarkably reduced when tested in water at 37 °C, which was above the glass transition temperature of polyGGE. At physiological conditions, entanglements of the ployGGE networks unfolded and the free volume of networks were replaced by water molecules as softener, which increased the mobility of the polymer chains, resulting in a lower Young's modulus. Protein adsorption analysis was performed on polyGGE films with 30 min UV curing using an enzyme-linked immunosorbent assay. PolyGGE could effectively prevent the adsorption of human plasma fibrinogen, albumin, and fibronectin at the interface of human plasma and polyGGE films. The protein resistance of polyGGE was comparable to the negative controls: the hemocompatible polydimethylsiloxane (PDMS), showing its potential as a coating material for cardiovascular implants. Moreover, antimicrobial tests of bacterial activity using isothermal microcalorimetry and the microscopic image of direct bacteria culturing demonstrated that polyGGE could directly interfere biofilm formation and growth of both Gram-negative and antibiotic-resistant Gram-positive bacteria, indicating the potential application of polyGGE for combating the risk of hospital-acquired infections and preventing drug-resistant superbug spreading. To investigate its cell compatibility, polyGGE films were extracted by different solvents (ethanol, chloroform, acetone) and cell culture medium. Indirect cytotoxicity tests showed extracted polyGGE films still had toxic effects on L929 fibroblast cells. High-performance liquid chromatography/electrospray ionization mass spectrometry revealed the occurrence of organochlorine-containing compounds released during the polymer-cell culture medium interaction. A constant level of those organochlorine-containing compounds was confirmed from GGE monomer by a specific peak of C-Cl stretching in infrared spectra of GGE. This is assumed to be the main reason causing the increased cell membrane permeability and decreased metabolic activity, leading to cell death. Attempts as changing solvents were made to remove toxic substances, however, the release of these small molecules seems to be sluggish. The densely crosslinked polyGGE networks can possibly contribute to the trapping of organochlorine-containing compounds. These results provide valuable information for exploring the potentially toxic substances, leaching from polyGGE networks, and propose a feasible strategy for minimizing the cytotoxicity via reducing their crosslinking density. Sulfamic acid/ N-Methyl-2-pyrrolidone (NMP) were selected as the reagents for the sulfation of polyGGE surfaces. Fourier transform attenuated total reflection infrared spectroscopy (ATR-FT-IR) was used to monitor the functionalization kinetics and the results confirmed the successful sulfate grafting on the surface of polyGGE with the covalent bond -C-O-S-. X-ray photoelectron spectroscopy was used to determine the element composition on the surface and the cross-section of the functionalized polyGGE and sulfation within 15 min guarantees the sulfation only takes place on the surface while not occurring in the bulk of the polymer. The concentration of grafted sulfates increased with the increasing reaction time. The hydrophilicity of the surface of polyGGE was highly increased due to the increase of negatively charged end groups. Three sterilization techniques including autoclaving, gamma irradiation, and ethylene oxide (EtO) sterilization were used for polyGGE sulfates. Results from ATR-FT-IR and Toluidine Blue O quantitative assay demonstrated the total loss of the sulfates after autoclave sterilization, which was also confirmed by the increased water contact angle. Little influence on the concentration of sulfates was found for gamma-irradiated and autoclaving sterilized polyGGE sulfates. To investigate the thermal influence on polyGGE sulfates, one strategy was to use poly(hydroxyethyl acrylate) sulfates (PHEAS) for modeling. The thermogravimetric analysis profile of PHEAS demonstrated that sulfates are not thermally stable independent of the substrate materials and decomposition of sulfates occurs at around 100 °C. Although gamma irradiation also showed little negative effect on the sulfate content, the color change in the polyGGE sulfates indicates chemical or physical change might occur in the polymer. EtO sterilization was validated as the most suitable sterilization technique to maintain the chemical structure of polyGGE sulfates. In conclusion, the conducted work proved that bulk polyGGE can be used as an antifouling coating material and shows its antimicrobial potential. Sulfates functionalization can be effectively realized using sulfamic acid/NMP. EtO sterilization is the most suitable sterilization technique for grafted sulfates. Besides, this thesis also offers a good strategy for the analysis of toxic leachable substances using suitable physicochemical characterization techniques. Future work will focus on minimizing/eliminating the release of toxic substances via reducing the crosslinking density. Another interesting aspect is to study whether grafted sulfates can meet the need for anti-thrombogenicity.},
  language  = {en}
}
@phdthesis{Ziege2022,
  author    = {Ziege, Ricardo},
  title     = {Growth dynamics and mechanical properties of E. coli biofilms},
  doi       = {10.25932/publishup-55986},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-559869},
  school      = {Universit{\"a}t Potsdam},
  pages     = {xi, 123},
  year      = {2022},
  abstract  = {Biofilms are complex living materials that form as bacteria get embedded in a matrix of self-produced protein and polysaccharide fibres. The formation of a network of extracellular biopolymer fibres contributes to the cohesion of the biofilm by promoting cell-cell attachment and by mediating biofilm-substrate interactions. This sessile mode of bacteria growth has been well studied by microbiologists to prevent the detrimental effects of biofilms in medical and industrial settings. Indeed, biofilms are associated with increased antibiotic resistance in bacterial infections, and they can also cause clogging of pipelines or promote bio-corrosion. However, biofilms also gained interest from biophysics due to their ability to form complex morphological patterns during growth. Recently, the emerging field of engineered living materials investigates biofilm mechanical properties at multiple length scales and leverages the tools of synthetic biology to tune the functions of their constitutive biopolymers. This doctoral thesis aims at clarifying how the morphogenesis of Escherichia coli (E. coli) biofilms is influenced by their growth dynamics and mechanical properties. To address this question, I used methods from cell mechanics and materials science. I first studied how biological activity in biofilms gives rise to non-uniform growth patterns. In a second study, I investigated how E. coli biofilm morphogenesis and its mechanical properties adapt to an environmental stimulus, namely the water content of their substrate. Finally, I estimated how the mechanical properties of E. coli biofilms are altered when the bacteria express different extracellular biopolymers. On nutritive hydrogels, micron-sized E. coli cells can build centimetre-large biofilms. During this process, bacterial proliferation and matrix production introduce mechanical stresses in the biofilm, which release through the formation of macroscopic wrinkles and delaminated buckles. To relate these biological and mechanical phenomena, I used time-lapse fluorescence imaging to track cell and matrix surface densities through the early and late stages of E. coli biofilm growth. Colocalization of high cell and matrix densities at the periphery precede the onset of mechanical instabilities at this annular region. Early growth is detected at this outer annulus, which was analysed by adding fluorescent microspheres to the bacterial inoculum. But only when high rates of matrix production are present in the biofilm centre, does overall biofilm spreading initiate along the solid-air interface. By tracking larger fluorescent particles for a long time, I could distinguish several kinematic stages of E. coli biofilm expansion and observed a transition from non-linear to linear velocity profiles, which precedes the emergence of wrinkles at the biofilm periphery. Decomposing particle velocities to their radial and circumferential components revealed a last kinematic stage, where biofilm movement is mostly directed towards the radial delaminated buckles, which verticalize. The resulting compressive strains computed in these regions were observed to substantially deform the underlying agar substrates. The co-localization of higher cell and matrix densities towards an annular region and the succession of several kinematic stages are thus expected to promote the emergence of mechanical instabilities at the biofilm periphery. These experimental findings are predicted to advance future modelling approaches of biofilm morphogenesis. E. coli biofilm morphogenesis is further anticipated to depend on external stimuli from the environment. To clarify how the water could be used to tune biofilm material properties, we quantified E. coli biofilm growth, wrinkling dynamics and rigidity as a function of the water content of the nutritive substrates. Time-lapse microscopy and computational image analysis revealed that substrates with high water content promote biofilm spreading kinetics, while substrates with low water content promote biofilm wrinkling. The wrinkles observed on biofilm cross-sections appeared more bent on substrates with high water content, while they tended to be more vertical on substrates with low water content. Both wet and dry biomass, accumulated over 4 days of culture, were larger in biofilms cultured on substrates with high water content, despite extra porosity within the matrix layer. Finally, the micro-indentation analysis revealed that substrates with low water content supported the formation of stiffer biofilms. This study shows that E. coli biofilms respond to the water content of their substrate, which might be used for tuning their material properties in view of further applications. Biofilm material properties further depend on the composition and structure of the matrix of extracellular proteins and polysaccharides. In particular, E. coli biofilms were suggested to present tissue-like elasticity due to a dense fibre network consisting of amyloid curli and phosphoethanolamine-modified cellulose. To understand the contribution of these components to the emergent mechanical properties of E. coli biofilms, we performed micro-indentation on biofilms grown from bacteria of several strains. Besides showing higher dry masses, larger spreading diameters and slightly reduced water contents, biofilms expressing both main matrix components also presented high rigidities in the range of several hundred kPa, similar to biofilms containing only curli fibres. In contrast, a lack of amyloid curli fibres provides much higher adhesive energies and more viscoelastic fluid-like material behaviour. Therefore, the combination of amyloid curli and phosphoethanolamine-modified cellulose fibres implies the formation of a composite material whereby the amyloid curli fibres provide rigidity to E. coli biofilms, whereas the phosphoethanolamine-modified cellulose rather acts as a glue. These findings motivate further studies involving purified versions of these protein and polysaccharide components to better understand how their interactions benefit biofilm functions. All three studies depict different aspects of biofilm morphogenesis, which are interrelated. The first work reveals the correlation between non-uniform biological activities and the emergence of mechanical instabilities in the biofilm. The second work acknowledges the adaptive nature of E. coli biofilm morphogenesis and its mechanical properties to an environmental stimulus, namely water. Finally, the last study reveals the complementary role of the individual matrix components in the formation of a stable biofilm material, which not only forms complex morphologies but also functions as a protective shield for the bacteria it contains. Our experimental findings on E. coli biofilm morphogenesis and their mechanical properties can have further implications for fundamental and applied biofilm research fields.},
  language  = {en}
}
@book{Ziegel2003,
  author    = {Ziegel, Andrea},
  title     = {Kurz- und langfristige Bestimmungsgr{\"u}nde f{\"u}r Parteiaffinit{\"a}ten in Ostdeutschland},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-10720},
  publisher      = {Universit{\"a}t Potsdam},
  year      = {2003},
  abstract  = {In dieser Arbeit wird den verschiedenen Thesen, die das Wahlverhalten in Ostdeutschland begr{\"u}nden, nachgegangen. Der Einbezug der politischen Kultur scheint als Erweiterung des sozialpsychologischen Ansatzes notwendig, um das W{\"a}hlerverhalten in den neuen Bundesl{\"a}ndern angemessen zu modellieren. F{\"u}r die Analyse der Parteiaffinit{\"a}ten werden die in den ersten beiden Kapiteln ausgearbeiteten Deutungen zu empirisch {\"u}berpr{\"u}fbaren Hypothesen verdichtet und anhand der im empirischen Teil der Arbeit aufgestellten Regressionsmodelle getestet. Inhalt: Die politische Kultur in Ostdeutschland - Besonderheiten politischer Einstellungen: Einstellungen zur Demokratie, Identit{\"a}tsabgrenzung - Problemwahrnehmungen und Kompetenzzuweisungen Ostdeutsches Wahlverhalten - Thesen - Politisierung der Sozialstruktur - Anwendbarkeit des Konzeptes Parteiidentifikation - Parteiidentifikation und Sozialstruktur - Sachthemen und Kandidaten Empirischer Test - Daten und Methode: Kausalmodell - Hypothesen - Ergebnisse f{\"u}r die einzelnen Parteien: CDU, SPD, PDS - Relatives Gewicht lang- und kurzfristiger Faktoren Fazit - Diskussion der Ergebnisse},
  language  = {de}
}
@article{ZieglerPfitznerSchulzetal.2022,
  author    = {Ziegler, Joceline and Pfitzner, Bjarne and Schulz, Heinrich and Saalbach, Axel and Arnrich, Bert},
  title     = {Defending against Reconstruction Attacks through Differentially Private Federated Learning for Classification of Heterogeneous Chest X-ray Data},
  series = {Sensors},
  volume    = {22},
  journal   = {Sensors},
  edition   = {14},
  publisher = {MDPI},
  address   = {Basel, Schweiz},
  issn      = {1424-8220},
  doi       = {10.3390/s22145195},
  pages     = {25},
  year      = {2022},
  abstract  = {Privacy regulations and the physical distribution of heterogeneous data are often primary concerns for the development of deep learning models in a medical context. This paper evaluates the feasibility of differentially private federated learning for chest X-ray classification as a defense against data privacy attacks. To the best of our knowledge, we are the first to directly compare the impact of differentially private training on two different neural network architectures, DenseNet121 and ResNet50. Extending the federated learning environments previously analyzed in terms of privacy, we simulated a heterogeneous and imbalanced federated setting by distributing images from the public CheXpert and Mendeley chest X-ray datasets unevenly among 36 clients. Both non-private baseline models achieved an area under the receiver operating characteristic curve (AUC) of 0.940.94 on the binary classification task of detecting the presence of a medical finding. We demonstrate that both model architectures are vulnerable to privacy violation by applying image reconstruction attacks to local model updates from individual clients. The attack was particularly successful during later training stages. To mitigate the risk of a privacy breach, we integrated R{\´e}nyi differential privacy with a Gaussian noise mechanism into local model training. We evaluate model performance and attack vulnerability for privacy budgets ε∈{1,3,6,10}�∈{1,3,6,10}. The DenseNet121 achieved the best utility-privacy trade-off with an AUC of 0.940.94 for ε=6�=6. Model performance deteriorated slightly for individual clients compared to the non-private baseline. The ResNet50 only reached an AUC of 0.760.76 in the same privacy setting. Its performance was inferior to that of the DenseNet121 for all considered privacy constraints, suggesting that the DenseNet121 architecture is more robust to differentially private training.},
  language  = {en}
}
@article{ZurellKoenigMalchowetal.2022,
  author    = {Zurell, Damaris and K{\"o}nig, Christian and Malchow, Anne-Kathleen and Kapitza, Simon and Bocedi, Greta and Travis, Justin M. J. and Fandos, Guillermo},
  title     = {Spatially explicit models for decision-making in animal conservation and restoration},
  series = {Ecography : pattern and diversity in ecology / Nordic Ecologic Society Oikos},
  journal   = {Ecography : pattern and diversity in ecology / Nordic Ecologic Society Oikos},
  number    = {4},
  publisher = {Wiley-Blackwell},
  address   = {Oxford},
  issn      = {1600-0587},
  doi       = {10.1111/ecog.05787},
  pages     = {1 -- 16},
  year      = {2022},
  abstract  = {Models are useful tools for understanding and predicting ecological patterns and processes. Under ongoing climate and biodiversity change, they can greatly facilitate decision-making in conservation and restoration and help designing adequate management strategies for an uncertain future. Here, we review the use of spatially explicit models for decision support and to identify key gaps in current modelling in conservation and restoration. Of 650 reviewed publications, 217 publications had a clear management application and were included in our quantitative analyses. Overall, modelling studies were biased towards static models (79\%), towards the species and population level (80\%) and towards conservation (rather than restoration) applications (71\%). Correlative niche models were the most widely used model type. Dynamic models as well as the gene-to-individual level and the community-to-ecosystem level were underrepresented, and explicit cost optimisation approaches were only used in 10\% of the studies. We present a new model typology for selecting models for animal conservation and restoration, characterising model types according to organisational levels, biological processes of interest and desired management applications. This typology will help to more closely link models to management goals. Additionally, future efforts need to overcome important challenges related to data integration, model integration and decision-making. We conclude with five key recommendations, suggesting that wider usage of spatially explicit models for decision support can be achieved by 1) developing a toolbox with multiple, easier-to-use methods, 2) improving calibration and validation of dynamic modelling approaches and 3) developing best-practise guidelines for applying these models. Further, more robust decision-making can be achieved by 4) combining multiple modelling approaches to assess uncertainty, and 5) placing models at the core of adaptive management. These efforts must be accompanied by long-term funding for modelling and monitoring, and improved communication between research and practise to ensure optimal conservation and restoration outcomes.},
  language  = {en}
}
@article{ZuehlkeMeilingRoderetal.2021,
  author    = {Z{\"u}hlke, Martin and Meiling, Till Thomas and Roder, Phillip and Riebe, Daniel and Beitz, Toralf and Bald, Ilko and L{\"o}hmannsr{\"o}ben, Hans-Gerd and Janßen, Traute and Erhard, Marcel and Repp, Alexander},
  title     = {Photodynamic inactivation of E. coli bacteria via carbon nanodots},
  series = {ACS omega / American Chemical Society},
  volume    = {6},
  journal   = {ACS omega / American Chemical Society},
  number    = {37},
  publisher = {ACS Publications},
  address   = {Washington, DC},
  issn      = {2470-1343},
  doi       = {10.1021/acsomega.1c01700},
  pages     = {23742 -- 23749},
  year      = {2021},
  abstract  = {The increasing development of antibiotic resistance in bacteria has been a major problem for years, both in human and veterinary medicine. Prophylactic measures, such as the use of vaccines, are of great importance in reducing the use of antibiotics in livestock. These vaccines are mainly produced based on formaldehyde inactivation. However, the latter damages the recognition elements of the bacterial proteins and thus could reduce the immune response in the animal. An alternative inactivation method developed in this work is based on gentle photodynamic inactivation using carbon nanodots (CNDs) at excitation wavelengths λex > 290 nm. The photodynamic inactivation was characterized on the nonvirulent laboratory strain Escherichia coli K12 using synthesized CNDs. For a gentle inactivation, the CNDs must be absorbed into the cytoplasm of the E. coli cell. Thus, the inactivation through photoinduced formation of reactive oxygen species only takes place inside the bacterium, which means that the outer membrane is neither damaged nor altered. The loading of the CNDs into E. coli was examined using fluorescence microscopy. Complete loading of the bacterial cells could be achieved in less than 10 min. These studies revealed a reversible uptake process allowing the recovery and reuse of the CNDs after irradiation and before the administration of the vaccine. The success of photodynamic inactivation was verified by viability assays on agar. In a homemade flow photoreactor, the fastest successful irradiation of the bacteria could be carried out in 34 s. Therefore, the photodynamic inactivation based on CNDs is very effective. The membrane integrity of the bacteria after irradiation was verified by slide agglutination and atomic force microscopy. The method developed for the laboratory strain E. coli K12 could then be successfully applied to the important avian pathogens Bordetella avium and Ornithobacterium rhinotracheale to aid the development of novel vaccines.},
  language  = {en}
}
@techreport{ŠedovaČizmaziovaCook2021,
  type      = {Working Paper},
  author    = {Šedov{\´a}, Barbora and Čizmaziov{\´a}, Lucia and Cook, Athene},
  title     = {A meta-analysis of climate migration literature},
  series = {CEPA Discussion Papers},
  journal   = {CEPA Discussion Papers},
  number    = {29},
  issn      = {2628-653X},
  doi       = {10.25932/publishup-49982},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-499827},
  pages     = {83},
  year      = {2021},
  abstract  = {The large literature that aims to find evidence of climate migration delivers mixed findings. This meta-regression analysis i) summarizes direct links between adverse climatic events and migration, ii) maps patterns of climate migration, and iii) explains the variation in outcomes. Using a set of limited dependent variable models, we meta-analyze thus-far the most comprehensive sample of 3,625 estimates from 116 original studies and produce novel insights on climate migration. We find that extremely high temperatures and drying conditions increase migration. We do not find a significant effect of sudden-onset events. Climate migration is most likely to emerge due to contemporaneous events, to originate in rural areas and to take place in middle-income countries, internally, to cities. The likelihood to become trapped in affected areas is higher for women and in low-income countries, particularly in Africa. We uniquely quantify how pitfalls typical for the broader empirical climate impact literature affect climate migration findings. We also find evidence of different publication biases.},
  language  = {en}
}
@book{OPUS4-721,
  title     = {Interdisciplinary studies on information structure : ISIS ; working papers of the SFB 632. - Vol. 1},
  editor    = {Ishihara, Shinichiro and Schmitz, Michaela and Schwarz, Anne},
  issn      = {1866-4725},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-8237},
  publisher      = {Universit{\"a}t Potsdam},
  year      = {2004},
  abstract  = {Contents: A1: Phonology and syntax of focussing and topicalisation: Gisbert Fanselow: Cyclic Phonology-Syntax-Interaction: Movement to First Position in German Caroline F{\´e}ry and Laura Herbst: German Sentence Accent Revisited Shinichiro Ishihara: Prosody by Phase: Evidence from Focus Intonation-Wh-scope Correspondence in Japanese A2: Quantification and information structure: Cornelia Endriss and Stefan Hinterwimmer: The Influence of Tense in Adverbial Quantification A3: Rhetorical Structure in Spoken Language: Modeling of Global Prosodic Parameters: Ekaterina Jasinskaja, J{\"o}rg Mayer and David Schlangen: Discourse Structure and Information Structure: Interfaces and Prosodic Realization B2: Focussing in African Tchadic languages: Katharina Hartmann and Malte Zimmermann: Focus Strategies in Chadic: The Case of Tangale Revisited D1: Linguistic database for information structure: Annotation and retrieval: Stefanie Dipper, Michael G{\"o}tze, Manfred Stede and Tillmann Wegst: ANNIS: A Linguistic Database for Exploring Information Structure},
  language  = {en}
}
@book{OPUS4-723,
  title     = {Heterogeneity in focus : creating and using linguistic databases},
  editor    = {Dipper, Stefanie and G{\"o}tze, Michael and Stede, Manfred},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  isbn      = {978-3-937786-48-3},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-8244},
  publisher      = {Universit{\"a}t Potsdam},
  pages     = {145},
  year      = {2005},
  abstract  = {The papers in this volume were presented at the workshop Heterogeneity in Linguistic Databases', which took place on July 9, 2004 at the University of Potsdam. The workshop was organized by project D1: Linguistic Database for Information Structure: Annotation and Retrieval', a member project of the SFB 632, a collaborative research center entitled Information Structure: the Linguistic Means for Structuring Utterances, Sentences and Texts'. The workshop brought together both developers and users of linguistic databases from a number of research projects which work on an empirical basis, all of which have to cope with different sorts of heterogeneity: primary linguistic data and annotated information may be heterogeneous, as well as the data structures representing them. The first four papers (by Wagner, Schmidt, L{\"u}deling, and Witt) address aspects of heterogeneous data from the point of view of database developers; the remaining three papers (by Meyer, Smith, and Teich/Fankhauser) focus on data exploitation by the users.},
  language  = {en}
}
@book{OPUS4-837,
  title     = {Handelsmarketing und st{\"a}dtische Handelsentwicklung : untersucht am Beispiel Potsdam},
  editor    = {Balderjahn, Ingo and Mennicken, Claudia},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-9299},
  publisher      = {Universit{\"a}t Potsdam},
  year      = {1996},
  abstract  = {Inhalt: Ulrike Franke, Gabriele Kr{\"a}tzer: Das Leitbild der Stadtentwicklung in Potsdam und die Bedeutung des Handels f{\"u}r die Stadtentwicklung Silvana Wagenitz: Rahmenbedingungen des Handels in und um Potsdam Torben Roffka: Vorhandene Handelsstrukturen und geplante Handelsprojekte in und um Potsdam Gesine Schmeichel: Perspektiven der Potsdamer Stadt- und Handelsentwicklung : Szenarien der Handelsentwicklung in Potsdam Cindy Kramer, Elke Radke: Perspektiven der Potsdamer Stadt- und Handelsentwicklung : Maßnahmen zur Steigerung der Attraktivit{\"a}t des inner- und außerst{\"a}dtischen Handels in Potsdam},
  language  = {de}
}
@book{OPUS4-1001,
  title     = {Image und Akzeptanz der Potsdamer Arkadien - eine empirische Analyse im Kulturmarketing},
  editor    = {Balderjahn, Ingo},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-10893},
  publisher      = {Universit{\"a}t Potsdam},
  year      = {1996},
  abstract  = {Dieser vorliegende Lehr- und Forschungsbericht des Lehrstuhls f{\"u}r Betriebswirtschaftslehre mit dem Schwerpunkt Marketing beschreibt ausgew{\"a}hlte Ergebnisse einer empirischen Studie zum Kulturmarketing. Inhalt: Erhebungssteckbrief Ziel der Studie und methodisches Vorgehen Erhebungsdesign und Stichprobenstruktur - Vorgehen bei der Erhebung - Probleme bei der Durchf{\"u}hrung der Interviews - Stichprobenstruktur - Eine Analyse der Besucherstrukturen der Potsdamer Arkadien und konkurrierender Kulturveranstaltungen Marktanalyse des Kulturangebots in Potsdam - Beschreibung der Wettbewerbssituation des Potsdamer Kulturangebots - Positionierung der Potsdamer Arkadien und anderer Kulturveranstaltungen aus Sicht der Veranstalter Darstellung der Ergebnisse - Bekanntheit der Potsdamer Arkadien - Das Entscheidungs- und Nutzungsverhalten von Besuchern der Potsdamer Arkadien und Besuchern konkurrierender Kulturveranstaltungen - Das lnformationsverhalten von Besuchern der Potsdamer Arkadien - St{\"a}rken und Schw{\"a}chen der Potsdamer Arkadien - Verbesserungsvorschl{\"a}ge - Zufriedenheit mit den Potsdamer Arkadien aus Sicht der Besucher - Zahlungsbereitschaftsanalyse von Besuchern der Potsdamer Arkadien - Erwartungen an Kulturveranstaltungen und Gr{\"u}nde f{\"u}r den Besuch der Potsdamer Arkadien - Einstellungen zu den Potsdamer Arkadien: Wichtigkeit und Bewertung von ausgew{\"a}hlten Merkmalen der Potsdamer Arkadien - Die Stadt Potsdam in der Wahrnehmung ihrer B{\"u}rger: Ermittlung des Wahrnehmungsraumes von Kulturveranstaltungen mittels der Korrespondenzanalyse - Typologisierung der Befragten nach ihren kulturellen Werteorientierungen},
  language  = {de}
}
@book{OPUS4-1092,
  title     = {"Bewegende R{\"a}ume" - Streiflichter multidisziplin{\"a}rer Raumverst{\"a}ndnisse : Festschrift anl{\"a}sslich der Verabschiedung von Frau Prof. Dr. Gabriele Saupe am 26.11.2004},
  editor    = {Ziener, Karen and Carstensen, Ines and Goltz, Elke},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  isbn      = {978-3-937786-80-3},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-11748},
  publisher      = {Universit{\"a}t Potsdam},
  pages     = {206},
  year      = {2005},
  abstract  = {Die vorliegende Festschrift wird Frau Prof. Gabrielle Saupe anl{\"a}sslich ihrer Verabschiedung aus dem Institut f{\"u}r Geographie der Universit{\"a}t Potsdam gewidmet. Bewegende R{\"a}ume - damit sind emotionale, materielle und strukturelle Bewegungen in einem r{\"a}umlichen Kontext gemeint. Die Vieldeutigkeit des Titels impliziert die Vielfalt der wissenschaftlichen Auseinandersetzung mit dem Raum. Ein illustres Autorenkollektiv deutscher Raumwissenschaftler verdeutlicht anhand von verschiedenen zum Teil essayartigen Artikeln sein Verst{\"a}ndnis von Raum. Die interdisziplin{\"a}re Konzeption des Heftes soll das st{\"a}ndige Bem{\"u}hen von Frau Saupe verdeutlichen, verschiedene Perspektiven, Herangehensweisen und Fachdisziplinen zur Bew{\"a}ltigung der Forschungsaufgaben zusammenzuf{\"u}hren. Hierbei sind die wichtigsten Forschungsprojekte von Frau Saupe ebenso vertreten wie einige der von ihr betreuten Qualifikationsarbeiten. Die Beitr{\"a}ge reichen von der Landschafts- bzw. Geo{\"o}kologie bis zum Fußball und der Musik. Sie spannen einen Bogen von der Geographie als Regionalwissenschaft zu modernen stadtsoziologischen Fragestellungen, stellen Ergebnisse neuerer Forschungsprojekte vor und widmen sich der Entwicklung von nahen und fernen R{\"a}umen aus verschiedenen Perspektiven.},
  language  = {de}
}
@book{OPUS4-816,
  title     = {Umweltmanagement bei kleinen und mittelst{\"a}ndischen Unternehmen : ausgew{\"a}hlte Ergebnisse einer empirischen Untersuchung},
  editor    = {Balderjahn, Ingo and Zanger, Cornelia and Gaus, Hansj{\"o}rg},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-9117},
  publisher      = {Universit{\"a}t Potsdam},
  year      = {1997},
  abstract  = {Die vorliegende Untersuchung basiert auf einer Befragung von insgesamt 93 Unternehmen aus den Regionen Potsdam, Berlin und Chemnitz. Die Interviews wurden im Zeitraum Mai/Juni 1996 durchgef{\"u}hrt und dauerten ca. 1 Stunde. Im Mittelpunkt der Befragung standen die Themenbereiche: {\"o}kologische Betroffenheit, Einstellung zum Umweltschutz, Markt- und Wettbewerbsbedingungen des betrieblichen Umweltschutzes, Umfang und Intensit{\"a}t betrieblicher Maßnahmen zum Umweltschutz sowie Verhaltensbarrieren. Als Interviewpartner standen in der Mehrzahl der befragten Unternehmen die Gesch{\"a}ftsf{\"u}hrer oder Umweltbeauftragte bzw. andere Personen in F{\"u}hrungspositionen zur Verf{\"u}gung. Zum {\"u}berwiegenden Teil waren die von uns befragten Unternehmer selbst Eigent{\"u}mer der Unternehmung. Die befragten Unternehmen geh{\"o}ren {\"u}berwiegend dem Konsumg{\"u}tersektor (44\%) an. Ein Drittel der Unternehmen stammt aus dem Investitionsg{\"u}tersektor (33\%). Der Dienstleistungssektor ist mit lediglich 23\% in der Stichprobe vertreten. Insgesamt bildet die Stichprobe ein breites Branchenspektrum ab, wenn auch die Gesch{\"a}ftsbereiche Maschinenbau und Anlagen, bedingt durch ihre starke Pr{\"a}senz im Raum Chemnitz, deutlich {\"u}berrepr{\"a}sentiert sind. Hinsichtlich der Besch{\"a}ftigtenzahlen und Ums{\"a}tze handelt es sich in der Befragung {\"u}berwiegend um kleine und mittelst{\"a}ndische Betriebe, wiewohl auch einige gr{\"o}ßere Unternehmen ber{\"u}cksichtigt wurden.},
  language  = {de}
}
@book{OPUS4-999,
  title     = {Das Image von Potsdam aus der Sicht lokaler Unternehmer},
  editor    = {Balderjahn, Ingo},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-10876},
  publisher      = {Universit{\"a}t Potsdam},
  year      = {1994},
  abstract  = {Dieser erste Lehr- und Forschungsbericht des Lehrstuhls f{\"u}r Betriebswirtschaftslehre mit dem Schwerpunkt Marketing beschreibt, analysiert und interpretiert ausgew{\"a}hlte Ergebnisse einer empirischen Studie zum lmage Potsdams bei lokalen Unternehmern. Inhalt: Die Studie im {\"U}berblick: Erhebungssteckbrief, Zusammenfassung der wichtigsten Ergebnisse Ziel der Studie Erhebungsdesign und Struktur der Stichprobe Das Image Potsdams als Wirtschaftsstandort - Die Standortfaktoren - Die Einsch{\"a}tzung der Standortfaktoren: Das {\"U}berzeugungsprofil - Die Wichtigkeit der Standortfaktoren: Das Anforderungsprofil - Die St{\"a}rken und Schw{\"a}chen Potsdams als Wirtschaftsstandort Typologisierung der lokalen Unternehmer nach ihrem lmage vom Wirtschaftsstandort Potsdam Das lmage Potsdams als Lebensraum f{\"u}r Unternehmer - Das lmageprofil der Stadt Potsdam als Lebensraum - Das 'Innere Bild' von der Stadt Potsdam Die wirtschaftliche Zukunft von Potsdam aus der Sicht der befragten Unternehmer Konsequenzen und Ansatzpunkte f{\"u}r die Gestaltung eines effizienten Regionalen Marketingkonzepts},
  language  = {de}
}
@book{OPUS4-5518,
  title     = {Personalmanagement in der brandenburgischen Landesverwaltung},
  editor    = {Salomon-Hengst, Annette and Kirchner, Steffi and Stumpenhusen, Susanne and Westphal, Volker-Gerd},
  publisher = {Universit{\"a}tsverlags Potsdam},
  address   = {Potsdam},
  isbn      = {978-3-86956-149-3},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-53846},
  publisher      = {Universit{\"a}t Potsdam},
  pages     = {345},
  year      = {2011},
  abstract  = {In der brandenburgischen Landesverwaltung und Justiz sollen in den n{\"a}chsten Jahren noch ca. 8.000 Stellen eingespart werden. Gleichzeitig sind die Landesverwaltung und die Justiz an die demografische Entwicklung und die damit verbundenen Aufgabenver{\"a}nderungen anzupassen. Das ressort{\"u}bergreifende Personalmanagement gewinnt hierdurch an Bedeutung. Um den Prozess zu gestalten, hat die Landesregierung eine Besetzungsrichtlinie erlassen und mit den Gewerkschaften einen Tarifvertrag zum Verwaltungsumbau (TV Umbau) abgeschlossen, die in diesem Band ebenso abgedruckt sind, wie die Durchf{\"u}hrungshinweise des Ministeriums des Innern. Dar{\"u}ber hinaus enth{\"a}lt der Band die aktuell geltende Personalbedarfsplanung bis Ende 2015, die Dr. Folke Schneider erl{\"a}utert. In einem Beitrag von Volker-Gerd Westphal wird eine Gesamtschau der Rahmenbedingungen f{\"u}r das Personalmanagement in der brandenburgischen Landesverwaltung und Justiz vorgenommen.},
  language  = {de}
}
@misc{OPUS4-41639,
  title     = {Dicion{\´a}rio de l{\´i}ngua geral amaz{\^o}nica},
  editor    = {Muller, Jean-Claude and Dietrich, Wolf and Monserrat, Ruth and Barros, C{\^a}ndida and Arenz, Karl-Heinz and Prudente, Gabriel},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  organization    = {Museu Paraense Emilio Goeldi},
  isbn      = {978-85-61377-99-1},
  doi       = {10.25932/publishup-41639},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-416390},
  pages     = {384},
  year      = {2019},
  abstract  = {Permettez {\`a} l'inventeur du manuscrit dont vous avez l'{\´e}dition diplomatique sous les yeux d'exposer en exergue les principales {\´e}tapes qui ont conduit en 2012 {\`a} la d{\´e}termination de la langue et {\`a} l'identification de l'auteur. {\´E}tant inscrit {\`a} la conf{\´e}rence de linguistique missionnaire {\`a} l'universit{\´e} de Br{\`e}me en mars 2012 et conscient que maintes biblioth{\`e}ques et archives renferment des tr{\´e}sors non identifi{\´e}s ou mal catalogu{\´e}s, je suivis le conseil d'un coll{\`e}gue de la biblioth{\`e}que de l'universit{\´e} de Tr{\`e}ves, Hans-Ulrich Seiffert, d'inspecter le manuscrit 1136 / 2048 4° de la biblioth{\`e}que municipale de Tr{\`e}ves. Cette biblioth{\`e}que est riche de plus de 2.500 manuscrits et incunables provenant pour la plupart des saisies r{\´e}volutionnaires fran{\c{c}}aises dans les biblioth{\`e}ques des ci-devant couvents tr{\´e}virois. Parmi ces tr{\´e}sors un manuscrit portugais des missions qui {\´e}tait rentr{\´e} dans les fonds de la biblioth{\`e}que de Tr{\`e}ves d{\`e}s 1799 et avait {\´e}t{\´e} expos{\´e} en 1991 dans le cadre d'une {\´e}vocation des activit{\´e}s missionnaires globales des J{\´e}suites. Michael Embach, alors directeur de la biblioth{\`e}que du s{\´e}minaire {\´e}piscopal de Tr{\`e}ves, l'avait bri{\`e}vement d{\´e}crit dans une notice qui pr{\´e}tendait qu'il {\´e}tait r{\´e}dig{\´e} en espagnol et en portugais et avait peut-{\^e}tre {\´e}t{\´e} compos{\´e} par un auteur « Meirin » dans le cadre de ses pr{\´e}parations pour partir dans les missions… Dans ma copie du catalogue de 1991 se trouve inscrit « la langue reste {\`a} d{\´e}terminer ». J'allais r{\´e}soudre le myst{\`e}re en cette m{\´e}morable journ{\´e}e du jeudi 23 f{\´e}vrier 2012 quand je me r{\´e}solus {\`a} l'autopsier dans la salle d{\´e}sormais historique de la Biblioth{\`e}que municipale de Tr{\`e}ves, dirig{\´e}e depuis peu par le coll{\`e}gue Embach. Il parut {\`a} l'{\´e}vidence que le manuscrit ne contenait aucun mot d'espagnol mais que dans la premi{\`e}re partie, les entr{\´e}es en portugais, strictement num{\´e}rot{\´e}es et arrang{\´e}es dans l'ordre alphab{\´e}tique, {\´e}taient suivies par leurs {\´e}quivalences dans une langue inconnue qui, cependant, avait pour un linguiste une forte odeur de l'Am{\´e}rique amazonienne. La partie post{\´e}rieure du manuscrit pr{\´e}sentait l'arrangement inverse. Les entr{\´e}es dans la langue inconnue {\´e}taient suivies de leurs {\´e}quivalences portugaises. Ce n'est que plus tard que nous avons compris le myst{\´e}rieux ordre des lemmata dans cette partie, bas{\´e}e qu'elle est sur la rime finale des mots. Deux facteurs ont grandement facilit{\´e} l'identification de la langue inconnue : 1. la permission du directeur Embach de pouvoir prendre des photos de travail de tous les folios du manuscrit et 2. l'entr{\´e}e dans le premier catalogue de la Biblioth{\`e}que de Tr{\`e}ves de 1802 : "Codex maxime memorabiblis est, cum nondum grammatica praeter Lusitani Anchieta nota sit, et nullum vocabularium huius linguae existet. Sine dubio scriptum est a quodam Missionario Jesuita". Rentrant de Tr{\`e}ves chez moi, je fis une br{\`e}ve halte dans mon bureau d'alors pour consulter Internet. L'entr{\´e}e "Anchieta" dans l'encyclop{\´e}die {\´e}lectronique Wikipedia m'informait que ce j{\´e}suite portugais avait compos{\´e} une grammaire du Vieux Tupi, publi{\´e}e en 1595. Sous le vocable "Old Tupi" ensuite, j'appris que l'auto-d{\´e}signation pour cette langue est {\~n}eengat{\´u} (la bonne langue, le parler correct). De l{\`a} {\`a} retrouver au folio 25 de mes photos la traduction du portugais linguagem par nhe{\´e}nga ne prit que quelques minutes. D{\`e}s ce premier soir donc, le myst{\`e}re de la langue inconnue {\´e}tait r{\´e}solu! Un projet de recherche international, voire transcontinental allait se mettre peu {\`a} peu en place apr{\`e}s. {\`A} la conf{\´e}rence de Br{\`e}me, d{\´e}but mars 2012, je fis la connaissance du linguiste et romaniste, le professeur Wolf Dietrich de l'universit{\´e} de M{\"u}nster en Westphalie, un des meilleurs connaisseurs des parlers amazoniens. Il rentrait avec une copie de mes photos de travail et confirma bient{\^o}t l'importance du manuscrit de Tr{\`e}ves pour la connaissance de la Lingua Geral, le parler qui s'{\´e}tait d{\´e}velopp{\´e} {\`a} partir du Vieux Tupi moribond. Je communiquai {\´e}galement ma d{\´e}couverte au fr{\`e}re Karl-Heinz Arenz de la congr{\´e}gation des p{\`e}res de Steyl, originaire de l'Eifel allemande et enseignant d'histoire aux universit{\´e}s br{\´e}siliennes de Bel{\´e}m et de Santar{\´e}m dans l'{\´E}tat du Par{\´a}. Arenz est l'auteur d'une {\´e}tude sur le j{\´e}suite luxembourgeois Jean-Philippe Bettendorf, actif au Maranh{\~a}o dans la seconde moiti{\´e} du XVIIe si{\`e}cle. Nous avions collabor{\´e} en 2007 {\`a} une exposition didactique consacr{\´e}e {\`a} ce personnage. Arenz ne tarda pas {\`a} passer l'information sur le manuscrit de Tr{\`e}ves aux deux linguistes br{\´e}siliennes C{\^a}ndida Barros et Ruth Monserrat, qui {\`a} leur tour pr{\´e}sentaient un projet de recherche qui fut accept{\´e} par les autorit{\´e}s br{\´e}siliennes. Les reals de ce subside furent bien investis en effet : l'{\´e}tudiant doctoral Gabriel Prudente r{\´e}alisa une transcription int{\´e}grale du manuscrit de Tr{\`e}ves qui fit appara{\^i}tre des couches dialectales et sociolectales dans le texte, et m{\^e}me quelques mots allemands. La biblioth{\`e}que municipale de Tr{\`e}ves, quant {\`a} elle, contribua « en nature » les images digitales qui sont consultables sur les pages de gauche de cette {\´e}dition {\´e}lectronique. D{\´e}but avril 2014, nous f{\^i}mes tous connaissance personnellement dans le cadre d'un colloque {\`a} l'universit{\´e} de Bel{\´e}m o{\`u} fut d{\´e}cid{\´e}e la pr{\´e}sente {\´e}dition digitale et o{\`u} furent prononc{\´e}es la plupart des contributions scientifiques que vous pouvez lire en guise d'introduction. Restait {\`a} percer l'{\´e}nigme du v{\´e}ritable auteur du manuscrit. C{\^a}ndida Barros avait assez t{\^o}t propos{\´e} les noms de trois J{\´e}suites allemands, actifs dans les ann{\´e}es 1750 dans la r{\´e}gion du fleuve Xingu et chass{\´e}s par les mesures anti-j{\´e}suites du pouvoir portugais en 1756. L'{\´e}tude pal{\´e}ographique des Quattuor vota des trois candidats, aux archives centrales des J{\´e}suites {\`a} Rome, me permit d{\´e}but septembre 2015 d'{\´e}liminer les p{\`e}res Eckart et Kaulen et de ne retenir que Antonius Meisterburg, originaire de Bernkastel sur la Moselle, comme le scribe du manuscrit de Tr{\`e}ves. Qu'il me soit permis d'exprimer ma GRATITUDE {\`a} toutes celles et {\`a} tous ceux qui ont contribu{\´e} {\`a} cette belle aventure de d{\´e}couverte intellectuelle et scientifique et {\`a} rendre au Br{\´e}sil une petite pierre de son histoire.},
  language  = {pt}
}
@inproceedings{OPUS4-1574,
  title     = {Clumping in hot-star winds : proceedings of an international workshop held in Potsdam, Germany, 18. - 22. June 2007},
  editor    = {Hamann, Wolf-Rainer and Feldmeier, Achim and Oskinova, Lida},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  isbn      = {978-3-940793-33-1},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-13981},
  pages     = {254},
  year      = {2007},
  abstract  = {Stellar winds play an important role for the evolution of massive stars and their cosmic environment. Multiple lines of evidence, coming from spectroscopy, polarimetry, variability, stellar ejecta, and hydrodynamic modeling, suggest that stellar winds are non-stationary and inhomogeneous. This is referred to as 'wind clumping'. The urgent need to understand this phenomenon is boosted by its far-reaching implications. Most importantly, all techniques to derive empirical mass-loss rates are more or less corrupted by wind clumping. Consequently, mass-loss rates are extremely uncertain. Within their range of uncertainty, completely different scenarios for the evolution of massive stars are obtained. Settling these questions for Galactic OB, LBV and Wolf-Rayet stars is prerequisite to understanding stellar clusters and galaxies, or predicting the properties of first-generation stars. In order to develop a consistent picture and understanding of clumped stellar winds, an international workshop on 'Clumping in Hot Star Winds' was held in Potsdam, Germany, from 18. - 22. June 2007. About 60 participants, comprising almost all leading experts in the field, gathered for one week of extensive exchange and discussion. The Scientific Organizing Committee (SOC) included John Brown (Glasgow), Joseph Cassinelli (Madison), Paul Crowther (Sheffield), Alex Fullerton (Baltimore), Wolf-Rainer Hamann (Potsdam, chair), Anthony Moffat (Montreal), Stan Owocki (Newark), and Joachim Puls (Munich). These proceedings contain the invited and contributed talks presented at the workshop, and document the extensive discussions.},
  language  = {en}
}