@article{WarnckeVogtUlrichetal.2004, author = {Warncke, Max and Vogt, Birgit and Ulrich, Jacqueline and von Laer, Meike Dorothee and Beyer, Winfried and Klump, Hannes and Micheel, Burkhard and Sheriff, Ahmed}, title = {Efficient in vitro transduction of naive murine B cells with lentiviral vectors}, year = {2004}, abstract = {The aim of this study was to determine the impact of lentiviral transduction on primary murine B cells. Studying B cell activities in vivo or using them for tolerance induction requires that the cells remain unaltered in their biological behavior except for expression of the transgene. As we show here, murine B cells can efficiently be transduced by lentiviral, VSV-G-pseudotyped vectors without the necessity of prior activation. Culture with LPS gave enhanced transduction efficiencies but led to the upregulation of CD86 and proliferation of the cells. Transduction of naive B cells by lentiviral vectors was dependent on multiplicity of infection and did not lead to a concomitant activation. Furthermore, the transduced cells could be used for studies in the NOD mouse system without altering the onset of diabetes. We conclude that lentiviral gene transfer into naive B cells is a powerful tool for manipulation of B cells for therapeutic applications.}, language = {en} } @article{VogtWarnckeMicheeletal.2009, author = {Vogt, Birgit and Warncke, Marit and Micheel, Burkhard and Sheriff, Ahmed}, title = {Lentiviral gene transfer of CTLA4 generates B cells with reduced costimulatory properties : brief definite report}, issn = {0891-6934}, doi = {10.1080/08916930902832470}, year = {2009}, abstract = {Peripheral T-cell (TC) tolerance can be induced by tolerogenic antigen-presenting cell (APC). A prerequisite is the reduction or blockade of B7 of APC. Besides dendritic cell, B cells can be used as APC. Here, we show the generation B cells with reduced B7 expression by lentiviral transduction of endoplasmic reticulum (ER)-directed CTLA4. Vectors coding for the human CTL4-Ig were used for the human B-cell line Raji. Transduction efficiency was over 90\% (MOI = 3). For the murine B-cell line A20 and for primary mouse B cells, murine CTLA4 was used. We show that B cells with reduced B7 expression reduce the antigen (Ag) specific TC proliferation in vitro. B cells expressing an ER-directed CTLA4 may reduce Ag-specific immune responses.}, language = {en} } @book{AkyaziBergmannDieterichsetal.2023, author = {Akyazi, Mehmet and Bergmann, Dana and Dieterichs, Michael and G{\"a}nz, Victoria and Hinz, Carsten and Huhn, Nicola and Klitsch, Constantin and Koch, Hayo and L{\"o}ffler, Robert and Meis, Robin and Penning, Isabelle and Rasky, Nicolai and Richter, Christin and Schellen, Ricarda and Schr{\"o}dter, Ayla and Schulz, Oliver and Simon, Veronika and Trojecka, Anetta and Verwohlt, Peter and Vogler, Christin and Vogt, Birgit}, title = {\#Gesellschaftslehre 9/10}, volume = {[Sch{\"u}lerband]}, publisher = {C.C. Buchner}, address = {Bamberg}, isbn = {978-3-661-70053-3}, pages = {464}, year = {2023}, language = {de} } @book{GaenzHinzHuhnetal.2022, author = {G{\"a}nz, Victoria and Hinz, Carsten and Huhn, Nicola and Klitsch, Constantin and L{\"o}ffler, Robert and Meis, Robin and Penning, Isabelle and Richter, Christin and Schellen, Ricarda and Schr{\"o}dter, Ayla and Schulz, Oliver and Simon, Veronika and Trojecka, Anetta and Verwohlt, Peter and Vogler, Christin and Vogt, Birgit}, title = {\#Gesellschaftslehre 7/8}, volume = {[Sch{\"u}lerband]}, publisher = {C.C. Buchner}, address = {Bamberg}, isbn = {978-3-661-70052-6}, pages = {400}, year = {2022}, language = {de} }