@article{SarauliRiedelWettsteinetal.2012, author = {Sarauli, David and Riedel, Marc and Wettstein, Christoph and Hahn, Robert and Stiba, Konstanze and Wollenberger, Ursula and Leimk{\"u}hler, Silke and Schmuki, Patrik and Lisdat, Fred}, title = {Semimetallic TiO2 nanotubes new interfaces for bioelectrochemical enzymatic catalysis}, series = {Journal of materials chemistry}, volume = {22}, journal = {Journal of materials chemistry}, number = {11}, publisher = {Royal Society of Chemistry}, address = {Cambridge}, issn = {0959-9428}, doi = {10.1039/c2jm16427b}, pages = {4615 -- 4618}, year = {2012}, abstract = {Different self-organized TiO2 nanotube structures are shown to represent new interfaces for the achievement of bioelectrochemical enzymatic catalysis involving redox proteins and enzymes without further surface modification or the presence of mediators.}, language = {en} } @article{WettsteinKanoSchaeferetal.2016, author = {Wettstein, Christoph and Kano, Kenji and Schaefer, Daniel and Wollenberger, Ursula and Lisdat, Fred}, title = {Interaction of Flavin-Dependent Fructose Dehydrogenase with Cytochrome c as Basis for the Construction of Biomacromolecular Architectures on Electrodes}, series = {Analytical chemistry}, volume = {88}, journal = {Analytical chemistry}, publisher = {American Chemical Society}, address = {Washington}, issn = {0003-2700}, doi = {10.1021/acs.analchem.6b00815}, pages = {6382 -- 6389}, year = {2016}, abstract = {The creation of electron transfer (ET) chains based on the defined arrangement of enzymes and redox proteins on electrode surfaces represents an interesting approach within the field of bioelectrocatalysis. In this study, we investigated the ET reaction of the flavin-dependent enzyme fructose dehydrogenase (FDH) with the redox protein cytochrome c (cyt c). Two different pH optima were found for the reaction in acidic and neutral solutions. When cyt c was adsorbed on an electrode surface while the enzyme remained in solution, ET proceeded efficiently in media of neutral pH. Interprotein ET was also observed in acidic media; however, it appeared to be less efficient. These findings suggest that two different ET pathways between the enzyme and cyt c may occur. Moreover, cyt c and FDH were immobilized in multiple layers on an electrode surface by means of another biomacromolecule: DNA (double stranded) using the layer -by -layer technique. The biprotein multilayer architecture showed a catalytic response in dependence on the fructose concentration, indicating that the ET reaction between both proteins is feasible even in the immobilized state. The electrode showed a defined response to fructose and a good storage stability. Our results contribute to the better understanding of the ET reaction between FDH and cyt c and provide the basis for the creation of all-biomolecule based fructose sensors the sensitivity of which can be controlled by the layer preparation.}, language = {en} } @article{SchellerWollenbergerWarsinkeetal.2001, author = {Scheller, Frieder W. and Wollenberger, Ursula and Warsinke, Axel and Lisdat, Fred}, title = {Research and development in biosensors}, year = {2001}, language = {en} } @article{FridmanWollenbergerBogdanovskayaetal.2000, author = {Fridman, Vadim and Wollenberger, Ursula and Bogdanovskaya, V. A. and Lisdat, Fred and Ruzgas, T. and Lindgren, A. and Gorton, Lo and Scheller, Frieder W.}, title = {Electrochemical investigation of cellobiose oxidation by cellobiose dehydrogenase in the presence of cytochrome c as mediator}, year = {2000}, language = {en} } @article{GeMeyerSchoeningetal.2000, author = {Ge, Bixia and Meyer, T. and Sch{\"o}ning, M. J. and Wollenberger, Ursula and Lisdat, Fred}, title = {Cytochrome c from chromatium vinosum on gold electrodes}, year = {2000}, language = {en} } @article{ChenWollenbergerLisdatetal.2000, author = {Chen, Jian and Wollenberger, Ursula and Lisdat, Fred and Ge, Bixia and Scheller, Frieder W.}, title = {Superoxide sensor based on hemin modified electrode}, year = {2000}, language = {en} } @article{SchellerJinEhrentreichFoersteretal.1999, author = {Scheller, Frieder W. and Jin, Wen and Ehrentreich-F{\"o}rster, Eva and Ge, Bixia and Lisdat, Fred and B{\"u}ttemeyer, R. and Wollenberger, Ursula}, title = {Cytochrome c based superoxide sensor for in vivo application}, year = {1999}, language = {en} } @article{LeiLisdatWollenbergeretal.1999, author = {Lei, Chenghong and Lisdat, Fred and Wollenberger, Ursula and Scheller, Frieder W.}, title = {Cytochrome c : Clay-modified electrode}, year = {1999}, language = {en} }