@article{KieferKrahlOsthoffetal.2017, author = {Kiefer, Thomas and Krahl, Dorothea and Osthoff, Kathrin and Thuss-Patience, Peter and Bunse, J{\"o}rg and Adam, Ulrich and Jansen, Marc H. and Ott, Rudolf and Pfitzmann, Robert and Pross, Matthias and Kohlmann, Thomas and Daeschlein, Georg and Buhlert, Hermann and V{\"o}ller, Heinz and Hirt, Carsten}, title = {Importance of Pancreatic Enzyme Replacement Therapy after Surgery of Cancer of the Esophagus or the Esophagogastric Junction}, series = {Nutrition and cancer : an international journal}, volume = {70}, journal = {Nutrition and cancer : an international journal}, number = {1}, publisher = {Routledge, Taylor \& Francis Group}, address = {Abingdon}, issn = {0163-5581}, doi = {10.1080/01635581.2017.1374419}, pages = {69 -- 72}, year = {2017}, abstract = {After surgical treatment of cancer of the esophagus or the esophagogastric junction we observed steatorrhea, which is so far seldom reported. We analyzed all patients treated in our rehabilitation clinic between 2011 and 2014 and focused on the impact of surgery on digestion of fat. Reported steatorrhea was anamnestic, no pancreatic function test was made. Here we show the results from 51 patients. Twenty-three (45\%) of the patients reported steatorrhea. Assuming decreased pancreatic function pancreatic enzyme replacement therapy (PERT) was started or modified during the rehabilitation stay (in the following called STEA(+)). These patients were compared with the patients without steatorrhea and without PERT (STEA(-)). Maximum weight loss between surgery and rehabilitation start was 18 kg in STEA(+) patient and 15.3 kg in STEA(-) patients. STEA(+) patients gained more weight under PERT during the rehabilitation phase (3 wk) than STEA(-) patients without PERT (+1.0 kg vs. -0.3 kg, P = 0.032). We report for the first time, that patients after cancer related esophageal surgery show anamnestic signs of exocrine pancreas insufficiency and need PERT to gain body weight.}, language = {en} } @article{KieferClaesNzayisengaetal.2015, author = {Kiefer, Christian S. and Claes, Andrea R. and Nzayisenga, Jean-Claude and Pietra, Stefano and Stanislas, Thomas and Ikeda, Yoshihisa and Grebe, Markus}, title = {Arabidopsis AIP1-2 restricted by WER-mediated patterning modulates planar polarity}, series = {Development}, journal = {Development}, number = {142}, doi = {doi: 10.1242/dev.111013}, pages = {151 -- 161}, year = {2015}, abstract = {The coordination of cell polarity within the plane of the tissue layer (planar polarity) is crucial for the development of diverse multicellular organisms. Small Rac/Rho-family GTPases and the actin cytoskeleton contribute to planar polarity formation at sites of polarity establishment in animals and plants. Yet, upstream pathways coordinating planar polarity differ strikingly between kingdoms. In the root of Arabidopsis thaliana, a concentration gradient of the phytohormone auxin coordinates polar recruitment of Rho-of-plant (ROP) to sites of polar epidermal hair initiation. However, little is known about cytoskeletal components and interactions that contribute to this planar polarity or about their relation to the patterning machinery. Here, we show that ACTIN7 (ACT7) represents a main actin isoform required for planar polarity of root hair positioning, interacting with the negative modulator ACTIN-INTERACTING PROTEIN1-2 (AIP1-2). ACT7, AIP1-2 and their genetic interaction are required for coordinated planar polarity of ROP downstream of ethylene signalling. Strikingly, AIP1-2 displays hair cell file-enriched expression, restricted by WEREWOLF (WER)-dependent patterning and modified by ethylene and auxin action. Hence, our findings reveal AIP1-2, expressed under control of the WER-dependent patterning machinery and the ethylene signalling pathway, as a modulator of actin-mediated planar polarity.}, language = {en} } @article{KieferVoellerNothroffetal.2019, author = {Kiefer, Thomas and V{\"o}ller, Heinz and Nothroff, J{\"o}rg and Schikora, Martin and von Podewils, Sebastian and Sicher, Claudia and Bartels-Reinisch, Birgit and Heyne, Karolin and Haase, Hermann and J{\"u}nger, Michael and Daeschlein, Georg}, title = {Multiresistente Erreger in der onkologischen und kardiologischen Rehabilitation}, series = {Die Rehabilitation : Zeitschrift f{\"u}r Praxis und Forschung in der Rehabilitation}, volume = {58}, journal = {Die Rehabilitation : Zeitschrift f{\"u}r Praxis und Forschung in der Rehabilitation}, number = {2}, publisher = {Thieme}, address = {Stuttgart}, issn = {0034-3536}, doi = {10.1055/a-0638-9226}, pages = {136 -- 142}, year = {2019}, abstract = {In der vorliegenden Studie wurde die Pr{\"a}valenz der Besiedlung mit multiresistenten Keimen an 155 Patienten aus der onkologischen und 157 Patienten aus der kardiologischen Rehabilitation mittels mikrobiologischen Screenings untersucht. Dabei zeigten 4,5\% der onkologischen und 4,5\% der kardiologischen Rehabilitationspatienten eine Besiedlung mit multiresistenten Erregern. Am h{\"a}ufigsten wurden 2-MRGN und ESBL (2,9\%) nachgewiesen. Onkologische Rehapatienten zeigten doppelt so hohe Pr{\"a}valenzraten f{\"u}r 3-MRGN im Vergleich zu kardiologischen (2,6 und 1,3\%). Insgesamt zeigen onkologische und kardiologische Rehabilitationspatienten vergleichsweise niedrige Pr{\"a}valenzraten f{\"u}r multiresistente Krankenhauskeime.}, language = {de} } @misc{KieferTrendelenburgVoellerNothroffetal.2017, author = {Kiefer-Trendelenburg, Thomas Hermann and V{\"o}ller, Heinz and Nothroff, J{\"o}rg and Schikora, Martin and Bartels-Reinisch, Birgit and Heyne, Karolin and Daeschlein, Georg}, title = {Prevalence of patients with multiresistant pathogens (MRP) in rehabilitation clinics}, series = {Oncology Research and Treatment}, volume = {40}, journal = {Oncology Research and Treatment}, publisher = {Karger}, address = {Basel}, issn = {2296-5270}, pages = {198 -- 198}, year = {2017}, language = {en} } @article{StanislasHuserBarbosaetal.2015, author = {Stanislas, Thomas and Huser, Anke and Barbosa, Ines C. R. and Kiefer, Christian S. and Brackmann, Klaus and Pietra, Stefano and Gustavsson, Anna and Zourelidou, Melina and Schwechheimer, Claus and Grebe, Markus}, title = {Arabidopsis D6PK is a lipid domain-dependent mediator of root epidermal planar polarity}, series = {Nature plants}, volume = {1}, journal = {Nature plants}, number = {11}, publisher = {Nature Publ. Group}, address = {London}, issn = {2055-026X}, doi = {10.1038/NPLANTS.2015.162}, pages = {9}, year = {2015}, abstract = {Development of diverse multicellular organisms relies on coordination of single-cell polarities within the plane of the tissue layer (planar polarity). Cell polarity often involves plasma membrane heterogeneity generated by accumulation of specific lipids and proteins into membrane subdomains. Coordinated hair positioning along Arabidopsis root epidermal cells provides a planar polarity model in plants, but knowledge about the functions of proteo-lipid domains in planar polarity signalling remains limited. Here we show that Rho-of-plant (ROP) 2 and 6, phosphatidylinositol-4-phosphate 5-kinase 3 (PIP5K3), DYNAMIN-RELATED PROTEIN (DRP) 1A and DRP2B accumulate in a sterol-enriched, polar membrane domain during root hair initiation. DRP1A, DRP2B, PIP5K3 and sterols are required for planar polarity and the AGCVIII kinase D6 PROTEIN KINASE (D6PK) is a modulator of this process. D6PK undergoes phosphatidylinositol-4,5-bisphosphate- and sterol-dependent basal-to-planar polarity switching into the polar, lipid-enriched domain just before hair formation, unravelling lipid-dependent D6PK localization during late planar polarity signalling.}, language = {en} } @article{KieferClaesNzayisengaetal.2015, author = {Kiefer, Christian S. and Claes, Andrea R. and Nzayisenga, Jean-Claude and Pietra, Stefano and Stanislas, Thomas and Hueser, Anke and Ikeda, Yoshihisa and Grebe, Markus}, title = {Arabidopsis AIP1-2 restricted by WER-mediated patterning modulates planar polarity}, series = {Development : Company of Biologists}, volume = {142}, journal = {Development : Company of Biologists}, number = {1}, publisher = {Company of Biologists Limited}, address = {Cambridge}, issn = {0950-1991}, doi = {10.1242/dev.111013}, pages = {151 -- 161}, year = {2015}, abstract = {The coordination of cell polarity within the plane of the tissue layer (planar polarity) is crucial for the development of diverse multicellular organisms. Small Rac/Rho-family GTPases and the actin cytoskeleton contribute to planar polarity formation at sites of polarity establishment in animals and plants. Yet, upstream pathways coordinating planar polarity differ strikingly between kingdoms. In the root of Arabidopsis thaliana, a concentration gradient of the phytohormone auxin coordinates polar recruitment of Rho-of-plant (ROP) to sites of polar epidermal hair initiation. However, little is known about cytoskeletal components and interactions that contribute to this planar polarity or about their relation to the patterning machinery. Here, we show that ACTIN7 (ACT7) represents a main actin isoform required for planar polarity of root hair positioning, interacting with the negative modulator ACTIN-INTERACTING PROTEIN1-2 (AIP1-2). ACT7, AIP1-2 and their genetic interaction are required for coordinated planar polarity of ROP downstream of ethylene signalling. Strikingly, AIP1-2 displays hair cell file-enriched expression, restricted by WEREWOLF (WER)-dependent patterning and modified by ethylene and auxin action. Hence, our findings reveal AIP1-2, expressed under control of the WER-dependent patterning machinery and the ethylene signalling pathway, as a modulator of actin-mediated planar polarity.}, language = {en} } @article{KieferKrahlHirtetal.2019, author = {Kiefer, Thomas and Krahl, Dorothea and Hirt, Carsten and V{\"o}ller, Heinz and Voelkel, Lorenz and Daeschlein, Georg}, title = {Influence of treatment caused impairments on anxiety and depression in patients with cancer of the Esophagus or the Esophagogastric junction}, series = {Journal of gastrointestinal cancer}, volume = {51}, journal = {Journal of gastrointestinal cancer}, number = {1}, publisher = {Springer}, address = {New York}, issn = {1941-6628}, doi = {10.1007/s12029-018-00193-7}, pages = {30 -- 34}, year = {2019}, abstract = {Purpose After therapy of cancer of the esophagus or the esophagogastric junction, patients often suffer from anxiety and depression. Some risk factors for elevated anxiety and depression are reported, but the influence of steatorrhea, the frequency of which has only recently been reported, has not yet been investigated. Method Using the Hospital Anxiety and Depression Scale (HADS), we analyzed the correlation of anxiety and depression with steatorrhea, appetite, and weight loss in 72 patients with cancer of the esophagus or of the esophagogastric junction, who were treated at our rehabilitation clinic between January 2011 and December 2014. In addition, effectiveness of psychological interviews was analyzed. Results We have evaluable anxiety questionnaires from 51 patients showing a median anxiety value of 5 (range 0-13). As for the depression, results from evaluable questionnaires of 54 patients also showed a median value of 5 (range 0-15). Increased anxiety and depression values (> 7) were observed in 25.4\% and 37.0\% of the patients respectively. Patients who were admitted with steatorrhea for rehabilitation showed a statistically higher anxiety value (median 6.3 vs. 4.7, p < 0.05), reduced appetite, and a weight loss above 15 kg depicting a correlation to anxiety and depression. Psychological conversations helped lowering the depression but had no influence on anxiety. Conclusions Impairments after cancer treatment, such as steatorrhea, appetite loss, and weight loss, should be interpreted as an alarm signal and should necessitate screening for increased anxiety and depression. Psychological therapy can help improving the extent of the depression.}, language = {en} }