@misc{HornConradGuentherSeipetal.2024, author = {Horn-Conrad, Antje and G{\"u}nther, Oliver and Seip, Juliane and Zimmermann, Matthias and Schuster, Stefanie and Himmler, Lena and Liebig, Ference and Engel, Silke and Scholz, Jana and B{\"a}hnisch, Marianna and Brosius-Gersdorf, Frauke and Agrofylax, Luisa and Limbach, Oliver and Peter, Stefanie and Ramm, Lina Marie and Kampe, Heike and Mikulla, Stefanie}, title = {Portal Transfer 2024}, series = {Portal Transfer: Alumni- und Transfermagazin der Universit{\"a}t Potsdam}, journal = {Portal Transfer: Alumni- und Transfermagazin der Universit{\"a}t Potsdam}, number = {2024}, issn = {2747-6898}, doi = {10.25932/publishup-64698}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-646984}, pages = {82}, year = {2024}, abstract = {Liebe Leserinnen und Leser, die eigene „Blase" verlassen, Perspektiven wechseln, Silo-Mentalit{\"a}t {\"u}berwinden - was der Wissenschaft in ihrem Innern gelingt, ja gelingen muss, um erfolgreich zu sein, stellt sie in ihrer Außenwirkung noch immer vor Herausforderungen. Dabei geh{\"o}rt es doch inzwischen zum Selbstverst{\"a}ndnis moderner Universit{\"a}ten, {\"o}ffentlich zu erkl{\"a}ren, woran in ihren R{\"a}umen geforscht wird, sich in gesellschaftliche Diskurse einzubringen und ihre Erkenntnisse z{\"u}gig in die Praxis zu {\"u}berf{\"u}hren. Die Universit{\"a}t Potsdam hat diese Transferaufgaben neben Lehre und Forschung als dritte S{\"a}ule installiert und ihrem Geb{\"a}ude damit noch mehr Stabilit{\"a}t verliehen. Seit Jahren geh{\"o}rt sie im nationalen Vergleich zu den erfolgreichsten Hochschulen, wenn es darum geht, Start-ups zu f{\"o}rdern und aus der Forschung heraus Unternehmen zu gr{\"u}nden: In diesem Magazin berichten wir von der Potassco Solutions GmbH des Informatikers Torsten Schaub, der mit seinem KI-System Clingo komplexe Optimierungsprobleme in Betrieben l{\"o}st. Oder von der SEQSTANT GmbH, die mit innovativer Diagnostik Erreger von Atemwegserkrankungen in Echtzeit bestimmen kann. Wir zeigen aber auch, wie Forschungsteams mit der Industrie kooperieren, zum Beispiel mit der K-UTEC im th{\"u}ringischen Sondershausen, um mit wissenschaftlichem Knowhow dazu beizutragen, dass dort in Produktionsabf{\"a}llen kein wertvolles Lithium verloren geht. Richtet sich der Technologietransfer vor allem an die Wirtschaft, so hilft der Wissenstransfer der gesamten Gesellschaft. Besonders stark ist die Universit{\"a}t Potsdam hier in der Bildung, denn mit ihren Lehramtsabsolventen schickt sie auch gleich den aktuellen Stand der Unterrichtsforschung in die Schulpraxis. Immer h{\"a}ufiger zieht dabei die Digitalisierung in die Klassenzimmer ein. Wie das gut gelingen kann, ist in diesem Magazin zu lesen. Zudem erkl{\"a}ren wir, was die Sportwissenschaft zur Therapie von Depressionen beitragen kann oder wie die Umweltforschung das Risikomanagement in von Hochwasser bedrohten Regionen verbessern will. Ob in {\"o}ffentlichen Verwaltungen oder politischen Institutionen - {\"u}berall ist wissenschaftliche Expertise gefragt. Wir zeigen das am Beispiel von Frauke Brosius-Gersdorf, die als Juristin die Bundesregierung zur Regulierung des Schwangerschaftsabbruchs ber{\"a}t. Der k{\"u}rzeste Weg des Wissens aus der Universit{\"a}t in die Praxis f{\"u}hrt zweifelsohne {\"u}ber die Alumni, die als Fach- und F{\"u}hrungskr{\"a}fte im Land und dar{\"u}ber hinaus wirksam werden. Dass dieser Weg schon w{\"a}hrend des Studiums beginnen kann, beweisen die vielen studentischen Initiativen, die hier zu Wort kommen. Sie alle scheuen nicht das Rampenlicht: ob bei Science Slams auf den B{\"u}hnen im Land Brandenburg, bei den TEDx-Talks im Hans Otto Theater, beim Kunst-Rundgang in der Potsdamer Waschhaus-Arena oder mit englischsprachigem Schauspiel an der Uni. {\"O}ffentlich in Erscheinung treten, neue Formen finden, um Wissen in die Breite der Bev{\"o}lkerung zu tragen - auch das geh{\"o}rt zum Transfer. Genau wie dieses Magazin.}, language = {de} } @article{WieseEsatbeyogluWinterhalteretal.2015, author = {Wiese, Stefanie and Esatbeyoglu, Tuba and Winterhalter, Peter and Kruse, Hans-Peter and Winkler, Stephanie and Bub, Achim and Kulling, Sabine E.}, title = {Comparative biokinetics and metabolism of pure monomeric, dimeric, and polymeric flavan-3-ols: A randomized cross-over study in humans}, series = {Molecular nutrition \& food research : bioactivity, chemistry, immunology, microbiology, safety, technology}, volume = {59}, journal = {Molecular nutrition \& food research : bioactivity, chemistry, immunology, microbiology, safety, technology}, number = {4}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {1613-4125}, doi = {10.1002/mnfr.201400422}, pages = {610 -- 621}, year = {2015}, abstract = {Scope: Flavan-3-ols are abundant polyphenols in human nutrition and are associated with beneficial health effects. The aim of this study was to comparatively investigate the metabolic fate of (-)-epicatechin, procyanidin B1, and polymeric procyanidins in a randomized cross-over study in humans. Methods and results: Parent compounds, conjugates, and microbial metabolites were determined in plasma, urine, and faeces by HPLC-MS and GC-MS/MS. Glucuronidated, sulfated, and methylated (-)-epicatechin and 5-(3',4'-dihydroxyphenyl)-valerolactone were the dominant metabolites in blood and urine. In addition, minor amounts of procyanidin B1 and 4-hydroxy-5-(3',4'-dihydroxyphenyl) valeric acid and their conjugated metabolites were detected. The formation of 5-(3',4'-dihydroxyphenyl)-valerolactone and 4-hydroxy-5-(3',4'-dihydroxyphenyl) valeric acid varied largely between individuals as well as with the degree of polymerization of flavan-3-ols. Monomer units were not detectable in plasma or urine after procyanidin B1 and polymeric procyanidin intake. No correlation was found between the intake of flavan-3-ols and the occurrence of phenolic acids in blood and urine or the phenolic compound profiles in faeces. Conclusion: In addition to conjugated metabolites derived from the absorption of monomeric flavan-3-ols, 5-(3',4' -dihydroxyphenyl)-valerolactone represents an important in vivo metabolite of (-)-epicatechin and procyanidin B1 produced by the gut microbiota.}, language = {en} } @article{GlaeserBolteMartinetal.2013, author = {Gl{\"a}ser, Stefanie P. and Bolte, Kathrin and Martin, Karin and Busse, Hans-J{\"u}rgen and Grossart, Hans-Peter and K{\"a}mpfer, Peter and Gl{\"a}ser, Jens}, title = {Novosphingobium fuchskuhlense sp nov., isolated from the north-east basin of Lake Grosse Fuchskuhle}, series = {International journal of systematic and evolutionary microbiology}, volume = {63}, journal = {International journal of systematic and evolutionary microbiology}, publisher = {Society for General Microbiology}, address = {Reading}, issn = {1466-5026}, doi = {10.1099/ijs.0.043083-0}, pages = {586 -- 592}, year = {2013}, abstract = {A yellow pigmented, Gram-negative, rod-shaped bacterium designated FNE08-7(T) was isolated from subsurface water of the north-east basin of the bog lake Grosse Fuchskuhle (Brandenburg, Germany). A first analysis of the nearly full-length 16S rRNA gene sequence analysis including environmental 16S rRNA gene sequences derived from freshwater ecosystems showed that strain FNE08-7(T) is the first cultured representative, to our knowledge, of the freshwater tribe Novo-A2. Further analysis indicates highest 16S rRNA gene sequence similarities to the type strains of Novosphingobium stygium (98.0\%) and Novosphingobium taihuense (97.4\%) and between 94.0\% and 96.9\% sequence similarity to other members of the genus Novosphingobium. Reconstruction of phylogenetic trees showed that strain FNE08-7(T) formed a distinct cluster with the type strains of N. stygium and N. taihuense supported by high bootstrap values. DNA DNA hybridization of strain FNE08-7(T) with N. stygium SMCC B0712(T) and N. taihuense DSM 17507(T) revealed low similarity values of 18.4\% (reciprocal: 11.4\%) and 23.1\% (reciprocal: 54.2\%), respectively. The predominant fatty acid of the isolate is C-18:1 omega 7c (56.4\%) and two characteristic 2-hydroxy fatty acids, C-14:0 2-OH (16.5\%) and C-15:0 2-OH (3.3\%) occur. Ubiquinone Q-10 is the major respiratory quinone. The predominant polar lipids are phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylglycerol, sphingoglycolipid, phosphatidylcholine and minor amounts of diphosphatidylglycerol. Spermidine is the predominant polyamine. Characterization by genotypic, chemotaxonomic and phenotypic analysis indicate that strain FNE08-7(T) represents a novel species of the genus Novosphingobium within the Alphaproteobacteria. Therefore, we propose the species Novosphingobium fuchskuhlense sp. nov., with FNE08-7(T) (=DSM 25065(T)=CCM 7978(T)=CCUG 61508(T)) as the type strain.}, language = {en} } @article{SchellenbergReichertHardtetal.2020, author = {Schellenberg, Johannes and Reichert, Jessica and Hardt, Martin and Klingelh{\"o}fer, Ines and Morlock, Gertrud and Schubert, Patrick and Bižić, Mina and Grossart, Hans-Peter and K{\"a}mpfer, Peter and Wilke, Thomas and Glaeser, Stefanie P.}, title = {The bacterial microbiome of the long-term aquarium cultured high-microbial abundance sponge Haliclona cnidata}, series = {Frontiers in Marine Science}, volume = {7}, journal = {Frontiers in Marine Science}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2296-7745}, doi = {10.3389/fmars.2020.00266}, pages = {20}, year = {2020}, abstract = {Marine sponges host highly diverse but specific bacterial communities that provide essential functions for the sponge holobiont, including antimicrobial defense. Here, we characterized the bacterial microbiome of the marine sponge Haliclona cnidata that has been in culture in an artificial marine aquarium system. We tested the hypotheses (1) that the long-term aquarium cultured sponge H. cnidata is tightly associated with a typical sponge bacterial microbiota and (2) that the symbiotic Bacteria sustain bioactivity under harmful environmental conditions to facilitate holobiont survival by preventing pathogen invasion. Microscopic and phylogenetic analyses of the bacterial microbiota revealed that H. cnidata represents a high microbial abundance (HMA) sponge with a temporally stable bacterial community that significantly shifts with changing aquarium conditions. A 4-week incubation experiment was performed in small closed aquarium systems with antibiotic and/or light exclusion treatments to reduce the total bacterial and photosynthetically active sponge-associated microbiota to a treatment-specific resilient community. While the holobiont was severely affected by the experimental treatment (i.e., bleaching of the sponge, reduced bacterial abundance, shifted bacterial community composition), the biological defense and bacterial community interactions (i.e., quorum sensing activity) remained intact. 16S rRNA gene amplicon sequencing revealed a resilient community of 105 bacterial taxa, which remained in the treated sponges. These 105 taxa accounted for a relative abundance of 72-83\% of the bacterial sponge microbiota of non-treated sponge fragments that have been cultured under the same conditions. We conclude that a sponge-specific resilient community stays biologically active under harmful environmental conditions, facilitating the resilience of the holobiont. In H. cnidata, bacteria are located in bacteriocytes, which may have contributed to the observed phenomenon.}, language = {en} } @book{BenderDreiackEngelsetal.2022, author = {Bender, Carsten and Dreiack, Stefanie and Engels, Victoria and Fisseler, Bj{\"o}rn and Gregory, Luisa and Gross, Monika and Kaffenberger, Jens and Kost{\"a}dt, Peter and Meyer zu Bexten, Erdmuthe and Rustemeier, Linda and Schwarz, Thorsten and Tannert, Benjamin and Velasquez, Estefania Cepeda and Weber, Gerhard}, title = {Leitfaden zur Digitalen Barrierefreiheit im Hochschulkontext}, series = {Arbeitspapier / Hochschulforum Digitalisierung (HFD) ; 66}, volume = {5}, journal = {Arbeitspapier / Hochschulforum Digitalisierung (HFD) ; 66}, publisher = {Stifterverband f{\"u}r die Deutsche Wissenschaft}, address = {Berlin}, issn = {2365-7081}, doi = {10.5445/IR/1000153177}, pages = {43}, year = {2022}, language = {de} } @article{vonLoeffelholzLieskeNeuschaeferRubeetal.2017, author = {von Loeffelholz, Christian and Lieske, Stefanie and Neuschaefer-Rube, Frank and Willmes, Diana M. and Raschzok, Nathanael and Sauer, Igor M. and K{\"o}nig, J{\"o}rg and Fromm, Martin F. and Horn, Paul and Chatzigeorgiou, Antonios and Pathe-Neuschaefer-Rube, Andrea and Jordan, Jens and Pfeiffer, Andreas F. H. and Mingrone, Geltrude and Bornstein, Stefan R. and Stroehle, Peter and Harms, Christoph and Wunderlich, F. Thomas and Helfand, Stephen L. and Bernier, Michel and de Cabo, Rafael and Shulman, Gerald I. and Chavakis, Triantafyllos and P{\"u}schel, Gerhard Paul and Birkenfeld, Andreas L.}, title = {The human longevity gene homolog INDY and interleukin-6 interact in hepatic lipid metabolism}, series = {Hepatology}, volume = {66}, journal = {Hepatology}, number = {2}, publisher = {Wiley}, address = {Hoboken}, issn = {0270-9139}, doi = {10.1002/hep.29089}, pages = {616 -- 630}, year = {2017}, abstract = {Reduced expression of the Indy ("I am Not Dead, Yet") gene in lower organisms promotes longevity in a manner akin to caloric restriction. Deletion of the mammalian homolog of Indy (mIndy, Slc13a5) encoding for a plasma membrane-associated citrate transporter expressed highly in the liver, protects mice from high-fat diet-induced and aging-induced obesity and hepatic fat accumulation through a mechanism resembling caloric restriction. We studied a possible role of mIndy in human hepatic fat metabolism. In obese, insulin-resistant patients with nonalcoholic fatty liver disease, hepatic mIndy expression was increased and mIndy expression was also independently associated with hepatic steatosis. In nonhuman primates, a 2-year high-fat, high-sucrose diet increased hepatic mIndy expression. Liver microarray analysis showed that high mIndy expression was associated with pathways involved in hepatic lipid metabolism and immunological processes. Interleukin-6 (IL-6) was identified as a regulator of mIndy by binding to its cognate receptor. Studies in human primary hepatocytes confirmed that IL-6 markedly induced mIndy transcription through the IL-6 receptor and activation of the transcription factor signal transducer and activator of transcription 3, and a putative start site of the human mIndy promoter was determined. Activation of the IL-6-signal transducer and activator of transcription 3 pathway stimulated mIndy expression, enhanced cytoplasmic citrate influx, and augmented hepatic lipogenesis in vivo. In contrast, deletion of mIndy completely prevented the stimulating effect of IL-6 on citrate uptake and reduced hepatic lipogenesis. These data show that mIndy is increased in liver of obese humans and nonhuman primates with NALFD. Moreover, our data identify mIndy as a target gene of IL-6 and determine novel functions of IL-6 through mINDY. Conclusion: Targeting human mINDY may have therapeutic potential in obese patients with nonalcoholic fatty liver disease. German Clinical Trials Register: DRKS00005450.}, language = {en} } @article{WoehlBruhnBadarBertzetal.2012, author = {W{\"o}hl-Bruhn, Stefanie and Badar, Muhammad and Bertz, Andreas and Tiersch, Brigitte and Koetz, Joachim and Menzel, Henning and M{\"u}ller, Peter P. and Bunjes, Heike}, title = {Comparison of in vitro and in vivo protein release from hydrogel systems}, series = {Journal of controlled release}, volume = {162}, journal = {Journal of controlled release}, number = {1}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0168-3659}, doi = {10.1016/j.jconrel.2012.05.049}, pages = {127 -- 133}, year = {2012}, abstract = {Hydrogel systems based on hydroxyethyl starch-polyethylene glycol methacrylate (HES-P(EG)(6)MA) or hydroxyethyl starch methacrylate (HES-MA) were used to assess the protein release behavior. Here, we analyzed the in vitro release of FITC-anti-human antibodies incorporated in either HES-P(EG)(6)MA or HES-MA hydrogel delivery systems in PBS or human serum. In addition, hydrogel disks and microparticles prepared from the two polymers were subcutaneously implanted in BALB/c mice. The in vivo release of FITC-IgG was non-invasively monitored by an in vivo imaging system (IVIS 200) over a time period of up to 3 months. The imaging system allowed to asses individual animals over time, therefore only a small number of animals was required to obtain high quality data. The reduction in fluorescence intensity at the site of administration was compared to in vitro release profiles. These investigations demonstrated a sustained release from HES-MA hydrogel disks compared to rapidly degrading HES-P(EG)(6)MA disks and microparticles. The sustained release from HES-MA disks could be further optimized by using increased polymer concentrations. Human serum as in vitro release medium reflected better the in vivo release from HES-P(EG)(6)MA systems than PBS, suggesting that the presence of organic substances like proteins or lipids may play a significant role for the release kinetics.}, language = {en} } @article{BoehnkeBrusbergKiermeierDrexler2010, author = {B{\"o}hnke, Dietmar and Brusberg-Kiermeier, Stefanie and Drexler, Peter}, title = {Introduction}, series = {Victorian highways, Victorian byways : new approaches to nineteenth century British literature and culture}, journal = {Victorian highways, Victorian byways : new approaches to nineteenth century British literature and culture}, publisher = {Trafo}, address = {Berlin}, isbn = {978-3-89626-939-3}, pages = {7 -- 11}, year = {2010}, language = {en} } @article{HaaseKrostSauteretal.2017, author = {Haase, Tobias and Krost, Annalena and Sauter, Tilman and Kratz, Karl and Peter, Jan and Kamann, Stefanie and Jung, Friedrich and Lendlein, Andreas and Zohlnh{\"o}fer, Dietlind and R{\"u}der, Constantin}, title = {In vivo biocompatibility assessment of poly (ether imide) electrospun scaffolds}, series = {Journal of Tissue Engineering and Regenerative Medicine}, volume = {11}, journal = {Journal of Tissue Engineering and Regenerative Medicine}, number = {4}, publisher = {Wiley}, address = {Hoboken}, issn = {1932-6254}, doi = {10.1002/term.2002}, pages = {1034 -- 1044}, year = {2017}, abstract = {Poly(ether imide) (PEI), which can be chemically functionalized with biologically active ligands, has emerged as a potential biomaterial for medical implants. Electrospun PEI scaffolds have shown advantageous properties, such as enhanced endothelial cell adherence, proliferation and low platelet adhesion in in vitro experiments. In this study, the in vivo behaviour of electrospun PEI scaffolds and PEI films was examined in a murine subcutaneous implantation model. Electrospun PEI scaffolds and films were surgically implanted subcutaneously in the dorsae of mice. The surrounding subcutaneous tissue response was examined via histopathological examination at 7 and 28days after implantation. No serious adverse events were observed for both types of PEI implants. The presence of macrophages or foreign body giant cells in the vicinity of the implants and the formation of a fibrous capsule indicated a normal foreign body reaction towards PEI films and scaffolds. Capsule thickness and inflammatory infiltration cells significantly decreased for PEI scaffolds during days 7-28 while remaining unchanged for PEI films. The infiltration of cells into the implant was observed for PEI scaffolds 7days after implantation and remained stable until 28days of implantation. Additionally some, but not all, PEI scaffold implants induced the formation of functional blood vessels in the vicinity of the implants. Conclusively, this study demonstrates the in vivo biocompatibility of PEI implants, with favourable properties of electrospun PEI scaffolds regarding tissue integration and wound healing.}, language = {en} } @article{WieseGaertnerRaweletal.2009, author = {Wiese, Stefanie and Gaertner, Sonja and Rawel, Harshadrai Manilal and Winterhalter, Peter and Kulling, Sabine E.}, title = {Protein interactions with cyanidin-3-glucoside and its influence on alpha-amylase activity}, issn = {0022-5142}, doi = {10.1002/Jsfa.3407}, year = {2009}, abstract = {BACKGROUND: Recent studies indicate that the bioavailability of anthocyanins is extremely low. One of the possible reasons could be their binding to proteins. Therefore, the binding affinity of cyanidin-3-glucoside (Cy3glc) to HSA and alpha-amylase was investigated by the quenching of protein tryptophan fluorescence. From data obtained, the binding constants and the free Gibbs energy were calculated. The changes in conformation of the proteins tested were studied with circular dichroism and the influence of binding on alpha-amylase activity determined. RESULTS: Cy3glc quenched the tryptophan fluorescence and upon ligand binding a change in protein structure was observed related to the corresponding decrease in the et-amylase activity. The association constants of 25 to 77 x 10(3) L mol(-1) were calculated for different proteins, indicating weak interactions of non-covalent nature. Competitive binding with HSA in the presence of 8-anilino-1-naphthalene sulfonic acid suggest involvement of hydrophobic interactions, in the case of HSA the possible site being subdomain IIA. CONCLUSION: The strongest affinity of Cy3glc for HSA being at pH 7 underlines its potential in transport and distribution of the phenolic compounds in organisms. An influence on salivary amylase activity is possible when drinking berry juices with high anthocyanins content.}, language = {en} } @article{WeinertWieseRaweletal.2012, author = {Weinert, Christoph H. and Wiese, Stefanie and Rawel, Harshadrai Manilal and Esatbeyoglu, Tuba and Winterhalter, Peter and Homann, Thomas and Kulling, Sabine E.}, title = {Methylation of catechins and procyanidins by rat and human Catechol-O-Methyltransferase metabolite profiling and molecular modeling studies}, series = {Drug metabolism and disposition : the biological fate of chemicals}, volume = {40}, journal = {Drug metabolism and disposition : the biological fate of chemicals}, number = {2}, publisher = {American Society for Pharmacology and Experimental Therapeutics}, address = {Bethesda}, issn = {0090-9556}, doi = {10.1124/dmd.111.041871}, pages = {353 -- 359}, year = {2012}, abstract = {Catechins and procyanidins are major polyphenols in plant-derived foods. Despite intensive studies in recent years, neither their biochemical nor their toxicological properties have been clarified sufficiently. This study aimed to compare the methylation of catechins and procyanidins by the enzyme catechol-O-methyltransferase (COMT) in vitro. We conducted incubations with rat liver cytosol and human placental cytosol including S-adenosyl-L-methionine. The set of substrates comprised the catechins (-)-epicatechin (EC) and (+)catechin (CAT), the procyanidin dimers B1, B2, B3, B4, B5, and B7 as well as procyanidin trimer C1. After extraction, metabolites were analyzed by means of liquid chromatography-electrospray ionizationmass spectrometry and liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry. EC and CAT were converted to two monomethylated metabolites each by human and rat COMT, with the 3'-O-methyl derivatives being consistently the main metabolites. Furthermore, the flavanyl units of procyanidins were methylated consecutively, leading to monomethylated and dimethylated dimeric metabolites as well as monomethylated, dimethylated, and trimethylated C1 metabolites. The methylation status of each flavanyl unit was determined by means of mass spectrometric quinone-methide fragmentation patterns. In addition, molecular modeling studies were performed with the aim to predict the preferred site of methylation and to verify the experimental data. In conclusion, our results indicate that the degree and position of methylation depend clearly on the three-dimensional structure of the entire substrate molecule.}, language = {en} } @article{BailisDillahuntMuelleretal.2017, author = {Bailis, Peter and Dillahunt, Tawanna and M{\"u}ller, Stefanie and Baudisch, Patrick}, title = {Research for Practice: Technology for Underserved Communities; Personal Fabrication}, series = {Communications of the ACM / Association for Computing Machinery}, volume = {60}, journal = {Communications of the ACM / Association for Computing Machinery}, publisher = {Association for Computing Machinery}, address = {New York}, issn = {0001-0782}, doi = {10.1145/3080188}, pages = {46 -- 49}, year = {2017}, abstract = {THIS INSTALLMENT OF Research for Practice provides curated reading guides to technology for underserved communities and to new developments in personal fabrication. First, Tawanna Dillahunt describes design considerations and technology for underserved and impoverished communities. Designing for the more than 1.6 billion impoverished individuals worldwide requires special consideration of community needs, constraints, and context. Her selections span protocols for poor-quality communication networks, community-driven content generation, and resource and public service discovery. Second, Stefanie Mueller and Patrick Baudisch provide an overview of recent advances in personal fabrication (for example, 3D printers).}, language = {en} } @book{MientusKlempinNowaketal.2023, author = {Mientus, Lukas and Klempin, Christiane and Nowak, Anna and Wyss, Corinne and Aufschnaiter, Claudia von and Faix, Ann-Christin and te Poel, Kathrin and Wahbe, Nadia and Pieper, Martin and H{\"o}ller, Katharina and Kallenbach, Lea and F{\"o}rster, Magdalena and Redecker, Anke and Dick, Mirjam and Holle, J{\"o}rg and Schneider, Edina and Rehfeldt, Daniel and Brauns, Sarah and Abels, Simone and Ferencik-Lehmkuhl, Daria and Fr{\"a}nkel, Silvia and Frohn, Julia and Liebsch, Ann-Catherine and Pech, Detlef and Schreier, Pascal and Jessen, Moiken and Großmann, Uta and Skintey, Lesya and Voerkel, Paul and Vaz Ferreira, Mergenfel A. and Zimmermann, Jan-Simon and Buddeberg, Magdalena and Henke, Vanessa and Hornberg, Sabine and V{\"o}lschow, Yvette and Warrelmann, Julia-Nadine and Malek, Jennifer and Tinnefeld, Anja and Schmidt, Peggy and Bauer, Tobias and J{\"a}nisch, Christopher and Spitzer, Lisa and Franken, Nadine and Degeling, Maria and Preisfeld, Angelika and Meier, Jana and K{\"u}th, Simon and Scholl, Daniel and Vogelsang, Christoph and Watson, Christina and Weißbach, Anna and Kulgemeyer, Christoph and Oetken, Mandy and Gorski, Sebastian and Kubsch, Marcus and Sorge, Stefan and Wulff, Peter and Fellenz, Carolin D. and Schnell, Susanne and Larisch, Cathleen and Kaiser, Franz and Knott, Christina and Reimer, Stefanie and Stegm{\"u}ller, Nathalie and Boukray{\^a}a Trabelsi, Kathrin and Schißlbauer, Franziska and Lemberger, Lukas and Barth, Ulrike and Wiehl, Angelika and Rogge, Tim and B{\"o}hnke, Anja and Dietz, Dennis and Großmann, Leroy and Wienmeister, Annett and Zoppke, Till and Jiang, Lisa and Gr{\"u}nbauer, Stephanie and Ostersehlt, D{\"o}rte and Peukert, Sophia and Sch{\"a}fer, Christoph and L{\"o}big, Anna and Br{\"o}ll, Leena and Brandt, Birgit and Breuer, Meike and Dausend, Henriette and Krelle, Michael and Andersen, Gesine and Falke, Sascha and Kindermann-G{\"u}zel, Kristin and K{\"o}rner, Katrina and Lottermoser, Lisa-Marie and P{\"u}gner, Kati and Sonnenburg, Nadine and Akarsu, Selim and Rechl, Friederike and Gadinger, Laureen and Heinze, Lena and Wittmann, Eveline and Franke, Manuela and Lachmund, Anne-Marie and B{\"o}ttger, Julia and Hannover, Bettina and Behrendt, Renata and Conty, Valentina and Grundmann, Stephanie and Ghassemi, Novid and Opitz, Ben and Br{\"a}mer, Martin and Gasparjan, David and Sambanis, Michaela and K{\"o}ster, Hilde and L{\"u}cke, Martin and Nordmeier, Volkhard and Schaal, Sonja and Haberbosch, Maximilian and Meissner, Maren and Schaal, Steffen and Br{\"u}chner, Melanie and Riehle, Tamara and Leopold, Bengta Marie and Gerlach, Susanne and Rau-Patschke, Sarah and Skorsetz, Nina and Weber, Nadine and Damk{\"o}hler, Jens and Elsholz, Markus and Trefzger, Thomas and Lewek, Tobias and Borowski, Andreas}, title = {Reflexion in der Lehrkr{\"a}ftebildung}, series = {Potsdamer Beitr{\"a}ge f{\"u}r Lehrkr{\"a}ftebildung und Bildungsforschung}, journal = {Potsdamer Beitr{\"a}ge f{\"u}r Lehrkr{\"a}ftebildung und Bildungsforschung}, number = {4}, editor = {Mientus, Lukas and Klempin, Christiane and Nowak, Anna}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-566-8}, issn = {2626-3556}, doi = {10.25932/publishup-59171}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-591717}, publisher = {Universit{\"a}t Potsdam}, pages = {452}, year = {2023}, abstract = {Reflexion ist eine Schl{\"u}sselkategorie f{\"u}r die professionelle Entwicklung von Lehrkr{\"a}ften, welche als Ausbildungsziel in den Bildungsstandards f{\"u}r die Lehrkr{\"a}ftebildung verankert ist. Eine Verstetigung universit{\"a}r gepr{\"a}gter Forschung und Modellierung in der praxisnahen Anwendung im schulischen Kontext bietet Potentiale nachhaltiger Professionalisierung. Die St{\"a}rkung reflexionsbezogener Kompetenzen durch Empirie und Anwendung scheint eine phasen{\"u}bergreifende Herausforderung der Lehrkr{\"a}ftebildung zu sein, die es zu bew{\"a}ltigen gilt. Ziele des Tagungsbandes Reflexion in der Lehrkr{\"a}ftebildung sind eine theoretische Sch{\"a}rfung des Konzeptes „Reflexive Professionalisierung" und der Austausch {\"u}ber Fragen der Einbettung wirksamer reflexionsbezogener Lerngelegenheiten in die Lehrkr{\"a}ftebildung. Forschende und Lehrende der‚ drei Phasen (Studium, Referendariat sowie Fort- und Weiterbildung) der Lehrkr{\"a}ftebildung stellen Lehrkonzepte und Forschungsprojekte zum Thema Reflexion in der Lehrkr{\"a}ftebildung vor und diskutieren diese. Gemeinsam mit Teilnehmenden aller Phasen und von verschiedenen Standorten der Lehrkr{\"a}ftebildung werden zuk{\"u}nftige Herausforderungen identifiziert und L{\"o}sungsans{\"a}tze herausgearbeitet.}, language = {de} } @book{AckermannAhlgrimmApelojgetal.2018, author = {Ackermann, Peter and Ahlgrimm, Frederik and Apelojg, Benjamin and B{\"o}rnert-Ringleb, Moritz and Borowski, Andreas and Ehlert, Antje and Eichler, Constanze and Frohn, Julia and Gehrmann, Marie-Luise and Gerlach, Erin and Goetz, Ilka and Goral, Johanna and Gronostaj, Anna and Grubert, Jana and G{\"u}lery{\"u}z, Burak and Hacke, Alexander and Heck, Sebastian and Hermanns, Jolanda and Hochmuth, J{\"o}rg and Jennek, Julia and Jostes, Brigitte and Jurczok, Anne and Kleemann, Katrin and Kortenkamp, Ulrich and Krauskopf, Karsten and K{\"u}choll, Denise and Kulawiak, Pawel R. and Lauterbach, Wolfgang and Lazarides, Rebecca and Linka, Tim and L{\"o}weke, Sebastian and Lohse-Bossenz, Hendrik and Maar, Verena and Nowak, Anna and Ratzlaff, Olaf and Reitz-Koncebovski, Karen and Rother, Stefanie and Scherreiks, Lynn and Schroeder, Christoph and Schwalbe, Anja and Schwill, Andreas and Tosch, Frank and Vock, Miriam and Wagner, Luisa and Westphal, Andrea and Wilbert, J{\"u}rgen}, title = {PSI-Potsdam}, series = {Potsdamer Beitr{\"a}ge zur Lehrerbildung und Bildungsforschung}, journal = {Potsdamer Beitr{\"a}ge zur Lehrerbildung und Bildungsforschung}, number = {1}, editor = {Borowski, Andreas and Ehlert, Antje and Prechtl, Helmut}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-442-5}, issn = {2626-3556}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-414542}, publisher = {Universit{\"a}t Potsdam}, pages = {354}, year = {2018}, abstract = {In Brandenburg kommt der Universit{\"a}t Potsdam eine besondere Rolle zu: Sie ist die einzige, an der zuk{\"u}nftige Lehrerinnen und Lehrer die erste Phase ihres Werdegangs - das Lehramtsstudium - absolvieren k{\"o}nnen. Vor diesem Hintergrund wurde bereits kurz nach der Gr{\"u}ndung im Jahr 1991 das „Potsdamer Modell der Lehrerbildung" entwickelt. Dieses Modell strebt fortlaufend eine enge Verzahnung von Theorie und Praxis {\"u}ber das gesamte Studium hinweg an und bindet hierf{\"u}r die schulpraktischen Studienanteile in besonderer Weise ein. Eine erneute St{\"a}rkung erfuhr die Lehrerbildung im Dezember 2014 mit der Gr{\"u}ndung des Zentrums f{\"u}r Lehrerbildung und Bildungsforschung (ZeLB). Aus der koordinierenden Arbeit des Zentrums entstand das fakult{\"a}ts{\"u}bergreifende Projekt „Professionalisierung - Schulpraktische Studien - Inklusion" (PSI-Potsdam) das im Rahmen der Qualit{\"a}tsoffensive Lehrerbildung des Bundesministeriums f{\"u}r Bildung und Forschung erfolgreich gef{\"o}rdert wurde (2015-2018) und dessen Verl{\"a}ngerung (2019-2023) bewilligt ist. Der vorliegende Band vermittelt in den drei großen Kapiteln „Erhebungsinstrumente", „Seminarkonzepte" und „Vernetzungen" einen {\"U}berblick {\"u}ber einige der praxisnahen Forschungszug{\"a}nge, hochschuldidaktischen Ans{\"a}tze und Strategien zur Vernetzung innerhalb der Lehrerbildung, die im Rahmen von PSI-Potsdam entwickelt und umgesetzt wurden. Die Beitr{\"a}ge wurden mit dem Ziel verfasst, Kolleginnen und Kollegen an Universit{\"a}ten und Hochschulen, Akteur_innen des Vorbereitungsdiensts sowie der Fort- und Weiterbildung von Lehrkr{\"a}ften m{\"o}glichst konkrete Einblicke zu gew{\"a}hren. Unter der Herausgeberschaft von Prof. Dr. Andreas Borowski (Fachdidaktik Physik), Prof. Dr. Antje Ehlert (Inklusionsp{\"a}dagogik mit dem F{\"o}rderschwerpunkt Lernen) und Prof. Dr. Helmut Prechtl (Fachdidaktik Biologie) vereinen sich Autor_innen mit breit gestreuter fachdidaktischer und bildungswissenschaftlicher Expertise.}, language = {de} } @book{HermannsBoehmeMeyeringetal.2023, author = {Hermanns, Jolanda and B{\"o}hme, Katrin and Meyering, Meike and Fuchs, Isabelle and Wagner, Simon and Krauskopf, Karsten and Knigge, Michel and Rother, Stefanie and Tosch, Frank and Wendland, Mirko and Wulff, Peter and Mientus, Lukas and Nowak, Anna and Borowski, Andreas and Baer, Ella and Bosch, Jannis and Wilbert, J{\"u}rgen and Br{\"a}sel, Tim and Fenn, Monika and Kortenkamp, Ulrich and Kuzle, Ana and Reitz-Koncebovski, Karen and Burg, Paula and Lampart, Fabian and Leubner, Martin and Freitag-Hild, Britta and Bitmann, Anna and Reinhardt, Susanne and Roos, Jana and Hußner, Isabell and B{\"o}rner, Dustin and Lazarides, Rebecca and Glowinski, Ingrid and Autenrieth, Marijke and Radke, Thea and Ehlert, Antje and Menke, Anne and Haupenthal, Anna and Schramm, Satyam Antonio and Kruse, Julia and K{\"o}rner, Dorothea and Fischer, Jakob Thomas and Kayser, Daniela Niesta}, title = {PSI-Potsdam}, series = {Potsdamer Beitr{\"a}ge f{\"u}r Lehrkr{\"a}ftebildung und Bildungsforschung}, journal = {Potsdamer Beitr{\"a}ge f{\"u}r Lehrkr{\"a}ftebildung und Bildungsforschung}, number = {3}, editor = {Hermanns, Jolanda}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-568-2}, issn = {2626-3556}, doi = {10.25932/publishup-60187}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-601875}, publisher = {Universit{\"a}t Potsdam}, pages = {393}, year = {2023}, abstract = {An der Universit{\"a}t Potsdam wird seit 2015 im Rahmen der „Qualit{\"a}tsoffensive Lehrerbildung" das Projekt „Professionalisierung - Schulpraktische Studien - Inklusion" (PSI-Potsdam) durchgef{\"u}hrt und am Zentrum f{\"u}r Lehrerbildung und Bildungsforschung (ZeLB) koordiniert. Zur ersten Projektf{\"o}rderphase (2015-2018) erschien der Band „PSI-Potsdam - Ergebnisbericht zu den Aktivit{\"a}ten im Rahmen der Qualit{\"a}tsoffensive Lehrerbildung (2015-2018)" zum Auftakt der Reihe „Potsdamer Beitr{\"a}ge zur Lehrerbildung und Bildungsforschung". Der vorliegende Band aus der gleichen Reihe gibt in den Kapiteln „Erhebungen", „Lehrkonzepte" und „Vernetzungen" einen {\"U}berblick {\"u}ber alle Teilprojekte der zweiten Projektf{\"o}rderphase (2019-2023). Wissenschaftler:innen aus verschiedenen Fachdidaktiken, Fachwissenschaften sowie aus den Bildungswissenschaften und der Inklusionsp{\"a}dagogik haben im Rahmen des Projektes kooperiert. Sowohl praxisnahe Forschung als auch die Entwicklung neuer Lehrkonzepte sowie Strategien zur Vernetzung innerhalb der Lehrkr{\"a}ftebildung stehen im Fokus dieses Bandes. Die Praxisphasen, die im Rahmen des „Potsdamer Modells der Lehrerbildung" eine zentrale Rolle spielen, wurden in einer großen Studie {\"u}ber alle Praxisphasen untersucht. Der Band gibt interessante Einblicke in die Ergebnisse der Teilprojekte und Anregungen sowohl f{\"u}r die eigene Forschung als auch f{\"u}r Entwicklungsarbeit wie zum Beispiel die Entwicklung neuer Lehrkonzepte. Herausgegeben wird dieser Band von PD Dr. Jolanda Hermanns (Gesamtkoordinatorin PSI-Potsdam und Chemiedidaktikerin).}, language = {de} } @book{JennekRotherToschetal.2022, author = {Jennek, Julia and Rother, Stefanie and Tosch, Frank and Wendland, Mirko and Kludt, Steffen and Krauskopf, Karsten and Kitschke, Dorothea and Maar, Verena and Knigge, Michel and Gn{\"a}dig, Susanne and Seidel, Astrid and Siehr, Karl-Heinz and Wienecke, Maik and G{\"u}nther, Claudia-Susanne and Reitz-Koncebovski, Karen and Klöpping, Peter M. and K{\"u}choll, Denise and Lazarides, Rebecca and Westphal, Andrea and Scherreiks, Lynn and Kuhr, Linda and Wilbert, J{\"u}rgen and Gronostaj, Anna and Vock, Miriam and Zaruba, Nicole and Ahlgrimm, Frederik and Link, J{\"o}rg-Werner and K{\"o}rner, Dorothea and Barseghyan, Anahit and Glowinski, Ingrid}, title = {Professionalisierung in Praxisphasen}, series = {Potsdamer Beitr{\"a}ge zur Lehrerbildung und Bildungsforschung}, journal = {Potsdamer Beitr{\"a}ge zur Lehrerbildung und Bildungsforschung}, number = {2}, editor = {Jennek, Julia}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, isbn = {978-3-86956-508-8}, issn = {2626-3556}, doi = {10.25932/publishup-50096}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-500964}, publisher = {Universit{\"a}t Potsdam}, pages = {321}, year = {2022}, abstract = {Schulpraktika bilden die zentrale Grundlage der Lehrerbildung in Potsdam. Bereits im Potsdamer Modell der Lehrerbildung (1993) sind sie festgehalten, seit der Integration des Schulpraktikums (Praxissemesters) 2008 absolvieren alle Potsdamer Lehramtsstudierenden f{\"u}nf Pflichtpraktika. W{\"a}hrend die Ziele der Praktika klar beschrieben sind, sind die tats{\"a}chlichen Lernerfolge nicht immer klar - ebenso wenig, wie die Begleitung der Praktika aussehen muss, um die Studierenden bestm{\"o}glich zu unterst{\"u}tzen. Auch die Integration in weitere Lehrveranstaltungen des Studiums ist ein noch offenes Feld, das weiterer Betrachtung verdient. Die unterschiedliche Ausrichtung der Potsdamer Praktika, Perspektivwechsel im Orientierungs-/Integriertem Eingangspraktikum, Selbstreflektion im Praktikum in p{\"a}dagogisch-psychologischen Handlungsfeldern, Unterricht als Profession in den Fachdidaktischen Tagespraktika, Anwendung von Diagnostik im psychodiagnostischen Praktikum und die Synthese all dessen im Schulpraktikum, bieten daf{\"u}r zahlreiche Ansatzpunkte. Schulpraktika sind nicht nur ein zentraler und von Studierenden hoch gesch{\"a}tzter Bestandteil des Studiums, sondern werden auch zunehmend f{\"u}r die Bildungsforschung interessant. Fragen nach der Kompetenzentwicklung, Selbsteinsch{\"a}tzungen und der Entwicklung der Reflexionsf{\"a}higkeit von Studierenden stehen dabei ebenso im Fokus wie die Einsch{\"a}tzung der universit{\"a}ren Begleitung und der Einbindung ins weitere Studium. Der vorliegende Band versammelt Studien von Wissenschaftlerinnen und Wissenschaftler der Universit{\"a}t Potsdam, die die f{\"u}nf Pflichtpraktika im Lehramtsstudium unter unterschiedlichen Blickwinkel beforschen. Besonders hervorzuheben ist, dass die Wissenschaftlerinnen und Wissenschaftler aus unterschiedlichen Disziplinen stammen und somit die Praktika mit verschiedenen Instrumenten und aus unterschiedlichen Blickwinkeln betrachten. Die pr{\"a}sentierten Ergebnisse bilden eine gute Grundlage, um die Praktika in Potsdam und an anderen Standorten weiterzuentwickeln.}, language = {de} } @article{GlaeserBerghoffStratmannetal.2014, author = {Glaeser, Stefanie P. and Berghoff, Bork A. and Stratmann, Verena and Grossart, Hans-Peter and Glaeser, Jens}, title = {Contrasting effects of singlet oxygen and hydrogen peroxide on bacterial community composition in a humic lake}, series = {PLoS one}, volume = {9}, journal = {PLoS one}, number = {3}, publisher = {PLoS}, address = {San Fransisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0092518}, pages = {19}, year = {2014}, abstract = {Light excitation of humic matter generates reactive oxygen species (ROS) in surface waters of aquatic ecosystems. Abundant ROS generated in humic matter rich lakes include singlet oxygen (O-1(2)) and hydrogen peroxide (H2O2). Because these ROS differ in half-life time and toxicity, we compared their effects on microbial activity (C-14-Leucine incorporation) and bacterial community composition (BCC) in surface waters of humic Lake Grosse Fuchskuhle (North-eastern Germany). For this purpose, experiments with water samples collected from the lake were conducted in July 2006, September 2008 and August 2009. Artificially increased O-1(2) and H2O2 concentrations inhibited microbial activity in water samples to a similar extent, but the effect of the respective ROS on BCC varied strongly. BCC analysis by 16S rRNA gene clone libraries and RT-PCR DGGE revealed ROS specific changes in relative abundance and activity of major bacterial groups and composition of dominating phylotypes. These changes were consistent in the three experiments performed in different years. The relative abundance of Polynucleobacter necessarius, Limnohabitans-related phylotypes (Betaproteobacteria), and Novosphingobium acidiphilum (Alphaproteobacteria) increased or was not affected by photo-sensitized O-1(2) exposure, but decreased after H2O2 exposure. The opposite pattern was found for Actinobacteria of the freshwater AcI-B cluster which were highly sensitive to O-1(2) but not to H2O2 exposure. Furthermore, group-specific RT-PCR DGGE analysis revealed that particle-attached P. necessarius and Limnohabitans-related phylotypes exhibit higher resistance to O-1(2) exposure compared to free-living populations. These results imply that O-1(2) acts as a factor in niche separation of closely affiliated Polynucleobacter and Limnohabitans-related phylotypes. Consequently, oxidative stress caused by photochemical ROS generation should be regarded as an environmental variable determining abundance, activity, and phylotype composition of environmentally relevant bacterial groups, in particular in illuminated and humic matter rich waters.}, language = {en} } @article{SchildgenRobinsonSavietal.2016, author = {Schildgen, Taylor F. and Robinson, Ruth A. J. and Savi, Sara and Phillips, William M. and Spencer, Joel Q. G. and Bookhagen, Bodo and Scherler, Dirk and Tofelde, Stefanie and Alonso, Ricardo N. and Kubik, Peter W. and Binnie, Steven A. and Strecker, Manfred}, title = {Landscape response to late Pleistocene climate change in NW Argentina: Sediment flux modulated by basin geometry and connectivity}, series = {Journal of geophysical research : Earth surface}, volume = {121}, journal = {Journal of geophysical research : Earth surface}, publisher = {American Geophysical Union}, address = {Washington}, issn = {2169-9003}, doi = {10.1002/2015JF003607}, pages = {392 -- 414}, year = {2016}, abstract = {Fluvial fill terraces preserve sedimentary archives of landscape responses to climate change, typically over millennial timescales. In the Humahuaca Basin of NW Argentina (Eastern Cordillera, southern Central Andes), our 29 new optically stimulated luminescence ages of late Pleistocene fill terrace sediments demonstrate that the timing of past river aggradation occurred over different intervals on the western and eastern sides of the valley, despite their similar bedrock lithology, mean slopes, and precipitation. In the west, aggradation coincided with periods of increasing precipitation, while in the east, aggradation coincided with decreasing precipitation or more variable conditions. Erosion rates and grain size dependencies in our cosmogenic Be-10 analyses of modern and fill terrace sediments reveal an increased importance of landsliding compared to today on the west side during aggradation, but of similar importance during aggradation on the east side. Differences in the timing of aggradation and the Be-10 data likely result from differences in valley geometry, which causes sediment to be temporarily stored in perched basins on the east side. It appears as if periods of increasing precipitation triggered landslides throughout the region, which induced aggradation in the west, but blockage of the narrow bedrock gorges downstream from the perched basins in the east. As such, basin geometry and fluvial connectivity appear to strongly influence the timing of sediment movement through the system. For larger basins that integrate subbasins with differing geometries or degrees of connectivity (like Humahuaca), sedimentary responses to climate forcing are likely attenuated.}, language = {en} } @misc{LemkeNeumeyerMihretal.2019, author = {Lemke, Stefanie and Neumeyer, Margarethe and Mihr, Anja and Steinbach, Peter and Weber, Johanna and Steinberg, Georg and John, Emanuel}, title = {MenschenRechtsMagazin : Informationen | Meinungen | Analysen}, volume = {23}, number = {2}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, issn = {1434-2820}, doi = {10.25932/publishup-42466}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-424664}, pages = {79}, year = {2019}, abstract = {Aus dem Inhalt: ▪ Human Rights Lawyering - das Stiefkind der Anwaltschaft ▪ Zum Problem der intertextuellen Auslegung von Menschenrechtsvertr{\"a}gen ▪ Bericht {\"u}ber die T{\"a}tigkeit des Menschenrechtsausschusses der Vereinten Nationen im Jahre 2017 - Teil II: Individualbeschwerden}, language = {de} } @article{CuiLoeberAlquezarPlanasetal.2016, author = {Cui, Pin and L{\"o}ber, Ulrike and Alquezar-Planas, David E. and Ishida, Yasuko and Courtiol, Alexandre and Timms, Peter and Johnson, Rebecca N. and Lenz, Dorina and Helgen, Kristofer M. and Roca, Alfred L. and Hartman, Stefanie and Greenwood, Alex D.}, title = {Comprehensive profiling of retroviral integration sites using target enrichment methods from historical koala samples without an assembled reference genome}, series = {PeerJ}, volume = {4}, journal = {PeerJ}, publisher = {PeerJ Inc.}, address = {London}, issn = {2167-8359}, doi = {10.7717/peerj.1847}, pages = {29}, year = {2016}, abstract = {Background. Retroviral integration into the host germline results in permanent viral colonization of vertebrate genomes. The koala retrovirus (KoRV) is currently invading the germline of the koala (Phascolarctos cinereus) and provides a unique opportunity for studying retroviral endogenization. Previous analysis of KoRV integration patterns in modern koalas demonstrate that they share integration sites primarily if they are related, indicating that the process is currently driven by vertical transmission rather than infection. However, due to methodological challenges, KoRV integrations have not been comprehensively characterized. Results. To overcome these challenges, we applied and compared three target enrichment techniques coupled with next generation sequencing (NGS) and a newly customized sequence-clustering based computational pipeline to determine the integration sites for 10 museum Queensland and New South Wales (NSW) koala samples collected between the 1870s and late 1980s. A secondary aim of this study sought to identify common integration sites across modern and historical specimens by comparing our dataset to previously published studies. Several million sequences were processed, and the KoRV integration sites in each koala were characterized. Conclusions. Although the three enrichment methods each exhibited bias in integration site retrieval, a combination of two methods, Primer Extension Capture and hybridization capture is recommended for future studies on historical samples. Moreover, identification of integration sites shows that the proportion of integration sites shared between any two koalas is quite small.}, language = {en} }