@article{KuhneDippongFlemigetal.2014, author = {Kuhne, Maren and Dippong, Martin and Flemig, Sabine and Hoffmann, Katrin and Petsch, Kristin and Schenk, J{\"o}rg A. and Kunte, Hans-J{\"o}rg and Schneider, Rudolf J.}, title = {Comparative characterization of mAb producing hapten-specific hybridoma cells by flow cytometric analysis and ELISA}, series = {Journal of immunological methods}, volume = {413}, journal = {Journal of immunological methods}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0022-1759}, doi = {10.1016/j.jim.2014.07.004}, pages = {45 -- 56}, year = {2014}, abstract = {A novel method that optimizes the screening for antibody-secreting hapten-specific hybridoma cells by using flow cytometry is described. Cell clones specific for five different haptens were analyzed. We selectively double stained and analyzed fixed hybridoma cells with fluorophore-labeled haptens to demonstrate the target-selectivity, and with a fluorophore-labeled anti-mouse IgG antibody to characterize the level of surface expression of membrane-bound IgGs. ELISA measurements with the supernatants of the individual hybridoma clones revealed that antibodies from those cells, which showed the highest fluorescence intensities in the flow cytometric analysis, also displayed the highest affinities for the target antigens. The fluorescence intensity of antibody-producing cells corresponded well with the produced antibodies' affinities toward their respective antigens. Immunohistochemical staining verified the successful double labeling of the cells. Our method makes it possible to perform a high-throughput screening for hybridoma cells, which have both an adequate IgG production rate and a high target affinity. (C) 2014 Elsevier B.V. All rights reserved.}, language = {en} } @article{DippongCarlLenzetal.2017, author = {Dippong, Martin and Carl, Peter and Lenz, Christine and Schenk, J{\"o}rg A. and Hoffmann, Katrin and Schwaar, Timm and Schneider, Rudolf J. and Kuhne, Maren}, title = {Hapten-Specific Single-Cell Selection of Hybridoma Clones by Fluorescence-Activated Cell Sorting for the Generation of Monoclonal Antibodies}, series = {Analytical chemistry}, volume = {89}, journal = {Analytical chemistry}, publisher = {American Chemical Society}, address = {Washington}, issn = {0003-2700}, doi = {10.1021/acs.analchem.6b04569}, pages = {4007 -- 4012}, year = {2017}, language = {en} } @article{TanskiBergstedtBevingtonetal.2019, author = {Tanski, George and Bergstedt, Helena and Bevington, Alexandre and Bonnaventure, Philip and Bouchard, Frederic and Coch, Caroline and Dumais, Simon and Evgrafova, Alevtina and Frauenfeld, Oliver W. and Frederick, Jennifer and Fritz, Michael and Frolov, Denis and Harder, Silvie and Hartmeyer, Ingo and Heslop, Joanne and Hoegstroem, Elin and Johansson, Margareta and Kraev, Gleb and Kuznetsova, Elena and Lenz, Josefine and Lupachev, Alexey and Magnin, Florence and Martens, Jannik and Maslakov, Alexey and Morgenstern, Anne and Nieuwendam, Alexandre and Oliva, Marc and Radosavljevi, Boris and Ramage, Justine Lucille and Schneider, Andrea and Stanilovskaya, Julia and Strauss, Jens and Trochim, Erin and Vecellio, Daniel J. and Weber, Samuel and Lantuit, Hugues}, title = {The Permafrost Young Researchers Network (PYRN) is getting older}, series = {Polar record}, volume = {55}, journal = {Polar record}, number = {4}, publisher = {Cambridge Univ. Press}, address = {New York}, issn = {0032-2474}, doi = {10.1017/S0032247418000645}, pages = {216 -- 219}, year = {2019}, abstract = {A lasting legacy of the International Polar Year (IPY) 2007-2008 was the promotion of the Permafrost Young Researchers Network (PYRN), initially an IPY outreach and education activity by the International Permafrost Association (IPA). With the momentum of IPY, PYRN developed into a thriving network that still connects young permafrost scientists, engineers, and researchers from other disciplines. This research note summarises (1) PYRN's development since 2005 and the IPY's role, (2) the first 2015 PYRN census and survey results, and (3) PYRN's future plans to improve international and interdisciplinary exchange between young researchers. The review concludes that PYRN is an established network within the polar research community that has continually developed since 2005. PYRN's successful activities were largely fostered by IPY. With >200 of the 1200 registered members active and engaged, PYRN is capitalising on the availability of social media tools and rising to meet environmental challenges while maintaining its role as a successful network honouring the legacy of IPY.}, language = {en} } @article{KupferBauervanRoesteletal.2022, author = {Kupfer, Thomas and Bauer, Evan B. and van Roestel, Jan and Bellm, Eric C. and Bildsten, Lars and Fuller, Jim and Prince, Thomas A. and Heber, Ulrich and Geier, Stephan and Green, Matthew J. and Kulkarni, Shrinivas R. and Bloemen, Steven and Laher, Russ R. and Rusholme, Ben and Schneider, David}, title = {Discovery of a Double-detonation Thermonuclear Supernova Progenitor}, series = {The astrophysical journal : an international review of spectroscopy and astronomical physics ; Part 2, Letters}, volume = {925}, journal = {The astrophysical journal : an international review of spectroscopy and astronomical physics ; Part 2, Letters}, number = {2}, publisher = {IOP Publ. Ltd.}, address = {Bristol}, issn = {2041-8205}, doi = {10.3847/2041-8213/ac48f1}, pages = {10}, year = {2022}, abstract = {We present the discovery of a new double-detonation progenitor system consisting of a hot subdwarf B (sdB) binary with a white dwarf companion with a P (orb) = 76.34179(2) minutes orbital period. Spectroscopic observations are consistent with an sdB star during helium core burning residing on the extreme horizontal branch. Chimera light curves are dominated by ellipsoidal deformation of the sdB star and a weak eclipse of the companion white dwarf. Combining spectroscopic and light curve fits, we find a low-mass sdB star, M (sdB) = 0.383 +/- 0.028 M (circle dot) with a massive white dwarf companion, M (WD) = 0.725 +/- 0.026 M (circle dot). From the eclipses we find a blackbody temperature for the white dwarf of 26,800 K resulting in a cooling age of approximate to 25 Myr whereas our MESA model predicts an sdB age of approximate to 170 Myr. We conclude that the sdB formed first through stable mass transfer followed by a common envelope which led to the formation of the white dwarf companion approximate to 25 Myr ago. Using the MESA stellar evolutionary code we find that the sdB star will start mass transfer in approximate to 6 Myr and in approximate to 60 Myr the white dwarf will reach a total mass of 0.92 M (circle dot) with a thick helium layer of 0.17 M (circle dot). This will lead to a detonation that will likely destroy the white dwarf in a peculiar thermonuclear supernova. PTF1 J2238+7430 is only the second confirmed candidate for a double-detonation thermonuclear supernova. Using both systems we estimate that at least approximate to 1\% of white dwarf thermonuclear supernovae originate from sdB+WD binaries with thick helium layers, consistent with the small number of observed peculiar thermonuclear explosions.}, language = {en} } @article{SchulzeMakuchWagnerKounavesetal.2018, author = {Schulze-Makuch, Dirk and Wagner, Dirk and Kounaves, Samuel P. and Mangelsdorf, Kai and Devine, Kevin G. and de Vera, Jean-Pierre and Schmitt-Kopplin, Philippe and Grossart, Hans-Peter and Parro, Victor and Kaupenjohann, Martin and Galy, Albert and Schneider, Beate and Airo, Alessandro and Froesler, Jan and Davila, Alfonso F. and Arens, Felix L. and Caceres, Luis and Cornejo, Francisco Solis and Carrizo, Daniel and Dartnell, Lewis and DiRuggiero, Jocelyne and Flury, Markus and Ganzert, Lars and Gessner, Mark O. and Grathwohl, Peter and Guan, Lisa and Heinz, Jacob and Hess, Matthias and Keppler, Frank and Maus, Deborah and McKay, Christopher P. and Meckenstock, Rainer U. and Montgomery, Wren and Oberlin, Elizabeth A. and Probst, Alexander J. and Saenz, Johan S. and Sattler, Tobias and Schirmack, Janosch and Sephton, Mark A. and Schloter, Michael and Uhl, Jenny and Valenzuela, Bernardita and Vestergaard, Gisle and Woermer, Lars and Zamorano, Pedro}, title = {Transitory microbial habitat in the hyperarid Atacama Desert}, series = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {115}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, number = {11}, publisher = {National Acad. of Sciences}, address = {Washington}, issn = {0027-8424}, doi = {10.1073/pnas.1714341115}, pages = {2670 -- 2675}, year = {2018}, language = {en} } @book{FladSchneiderSchulze2007, author = {Flad, H.-J. and Schneider, R. and Schulze, Bert-Wolfgang}, title = {Asymptotic Regularity of Solutions of Hartree-Fock Equations with Coulomb Potential}, series = {Preprint / Universit{\"a}t Potsdam, Institut f{\"u}r Mathematik, Arbeitsgruppe Partiell}, journal = {Preprint / Universit{\"a}t Potsdam, Institut f{\"u}r Mathematik, Arbeitsgruppe Partiell}, publisher = {Univ.}, address = {Potsdam}, issn = {1437-739X}, pages = {26 S.}, year = {2007}, language = {en} }