@article{KramerSchadtNiedballaPilgrimetal.2013, author = {Kramer-Schadt, Stephanie and Niedballa, J{\"u}rgen and Pilgrim, John D. and Schr{\"o}der-Esselbach, Boris and Lindenborn, Jana and Reinfelder, Vanessa and Stillfried, Milena and Heckmann, Ilja and Scharf, Anne K. and Augeri, Dave M. and Cheyne, Susan M. and Hearn, Andrew J. and Ross, Joanna and Macdonald, David W. and Mathai, John and Eaton, James and Marshall, Andrew J. and Semiadi, Gono and Rustam, Rustam and Bernard, Henry and Alfred, Raymond and Samejima, Hiromitsu and Duckworth, J. W. and Breitenmoser-Wuersten, Christine and Belant, Jerrold L. and Hofer, Heribert and Wilting, Andreas}, title = {The importance of correcting for sampling bias in MaxEnt species distribution models}, series = {Diversity \& distributions : a journal of biological invasions and biodiversity}, volume = {19}, journal = {Diversity \& distributions : a journal of biological invasions and biodiversity}, number = {11}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {1366-9516}, doi = {10.1111/ddi.12096}, pages = {1366 -- 1379}, year = {2013}, abstract = {AimAdvancement in ecological methods predicting species distributions is a crucial precondition for deriving sound management actions. Maximum entropy (MaxEnt) models are a popular tool to predict species distributions, as they are considered able to cope well with sparse, irregularly sampled data and minor location errors. Although a fundamental assumption of MaxEnt is that the entire area of interest has been systematically sampled, in practice, MaxEnt models are usually built from occurrence records that are spatially biased towards better-surveyed areas. Two common, yet not compared, strategies to cope with uneven sampling effort are spatial filtering of occurrence data and background manipulation using environmental data with the same spatial bias as occurrence data. We tested these strategies using simulated data and a recently collated dataset on Malay civet Viverra tangalunga in Borneo. LocationBorneo, Southeast Asia. MethodsWe collated 504 occurrence records of Malay civets from Borneo of which 291 records were from 2001 to 2011 and used them in the MaxEnt analysis (baseline scenario) together with 25 environmental input variables. We simulated datasets for two virtual species (similar to a range-restricted highland and a lowland species) using the same number of records for model building. As occurrence records were biased towards north-eastern Borneo, we investigated the efficacy of spatial filtering versus background manipulation to reduce overprediction or underprediction in specific areas. ResultsSpatial filtering minimized omission errors (false negatives) and commission errors (false positives). We recommend that when sample size is insufficient to allow spatial filtering, manipulation of the background dataset is preferable to not correcting for sampling bias, although predictions were comparatively weak and commission errors increased. Main ConclusionsWe conclude that a substantial improvement in the quality of model predictions can be achieved if uneven sampling effort is taken into account, thereby improving the efficacy of species conservation planning.}, language = {en} } @article{NeumannKielbRustametal.2017, author = {Neumann, Bettina and Kielb, Patrycja and Rustam, Lina and Fischer, Anna and Weidinger, Inez M. and Wollenberger, Ulla}, title = {Bioelectrocatalytic Reduction of Hydrogen Peroxide by Microperoxidase-11 Immobilized on Mesoporous Antimony-Doped Tin Oxide}, series = {ChemElectrChem}, volume = {4}, journal = {ChemElectrChem}, number = {4}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {2196-0216}, doi = {10.1002/celc.201600776}, pages = {913 -- 919}, year = {2017}, abstract = {The heme-undecapeptide microperoxidase-11 (MP-11) was immobilized on mesoporous antimony-doped tin oxide (ATO) thin-film electrodes modified with the positively charged binding promotor polydiallyldimethylammonium chloride. Surface concentrations of MP-11 of 1.5 nmol cm(-2) were sufficiently high to enable spectroelectrochemical analyses. UV/Vis spectroscopy and resonance Raman spectroscopy revealed that immobilized MP-11 adopts a six-coordinated low-spin conformation, as in solution in the presence of a polycation. Cathodic reduction of hydrogen peroxide at potentials close to +500mV versus Ag/AgCl indicates that the reaction proceeds via a Compound I-type like intermediate, analogous to natural peroxidases, and confirms mesoporous ATO as a suitable host material for adsorbing the heme-peptide in its native state. A hydrogen peroxide sensor is proposed by using the bioelectrocatalytic properties of the MP-11-modified ATO.}, language = {en} } @article{JetzschmannYarmanRustametal.2018, author = {Jetzschmann, Katharina J. and Yarman, Aysu and Rustam, L. and Kielb, P. and Urlacher, V. B. and Fischer, A. and Weidinger, I. M. and Wollenberger, Ulla and Scheller, Frieder W.}, title = {Molecular LEGO by domain-imprinting of cytochrome P450 BM3}, series = {Colloids and surfaces : an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin ; B, Biointerfaces}, volume = {164}, journal = {Colloids and surfaces : an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin ; B, Biointerfaces}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0927-7765}, doi = {10.1016/j.colsurfb.2018.01.047}, pages = {240 -- 246}, year = {2018}, abstract = {Hypothesis: Electrosynthesis of the MIP nano-film after binding of the separated domains or holocytochrome BM3 via an engineered anchor should result in domain-specific cavities in the polymer layer. Experiments: Both the two domains and the holo P450 BM3 have been bound prior polymer deposition via a N-terminal engineered his6-anchor to the electrode surface. Each step of MIP preparation was characterized by cyclic voltammetry of the redox-marker ferricyanide. Rebinding after template removal was evaluated by quantifying the suppression of the diffusive permeability of the signal for ferricyanide and by the NADH-dependent reduction of cytochrome c by the reductase domain (BMR). Findings: The working hypothesis is verified by the discrimination of the two domains by the respective MIPs: The holoenzyme P450 BM3 was ca. 5.5 times more effectively recognized by the film imprinted with the oxidase domain (BMO) as compared to the BMR-MIP or the non-imprinted polymer (NIP). Obviously, a cavity is formed during the imprinting process around the hiss-tag-anchored BMR which cannot accommodate the broader BMO or the P450 BM3. The affinity of the MIP towards P450 BM3 is comparable with that to the monomer in solution. The hiss-tagged P450 BM3 binds (30 percent) stronger which shows the additive effect of the interaction with the MIP and the binding to the electrode.}, language = {en} } @article{SchroederWarloHerrmannetal.1999, author = {Schr{\"o}der, Rolf and Warlo, Irene and Herrmann, Harald and VanDerVen, Peter F. M and Klasen, Christian and Bl{\"u}mke, Ingmar and Mundegar, Rustam R. and F{\"u}rst, Dieter Oswald and G{\"o}bel, Hans H. and Magin, Thomas}, title = {Immunogold EM reveals a close association of plectin and the desmin cytoskeleton in human skeletal muscle}, year = {1999}, language = {en} }