@article{BaesslerWeissWienkoopetal.2009, author = {Baessler, Olivia Y. and Weiss, Julia and Wienkoop, Stefanie and Lehmann, Karola and Scheler, Christian and Doelle, Sabine and Schwarz, Dietmar and Franken, Philipp and George, Eckhard and Worm, Margitta and Weckwerth, Wolfram}, title = {Evidence for novel tomato seed allergens : IgE-reactive legumin and vicilin proteins identified by multidimensional protein fractionation-mass spectrometry and in silico epitope modeling}, issn = {1535-3893}, doi = {10.1021/Pr800186d}, year = {2009}, abstract = {Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomato-sensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato fruit protein. For the molecular characterization of tomato seed allergens, a multidimensional protein fractionation strategy and LC-MS/MS was used. Two legumin- and vicilin-proteins were purified and showed strong IgE-reactivity in immunoblots. Individual patient sera exhibited varying IgE-sensitivity against the purified proteins. In silico structural modeling indicates high homology between epitopes of known walnut allergens and the detected IgE-crossreactive tomato proteins.}, language = {en} } @article{CosmeFrankenMewisetal.2014, author = {Cosme, Marco and Franken, Philipp and Mewis, Inga and Baldermann, Susanne and Wurst, Susanne}, title = {Arbuscular mycorrhizal fungi affect glucosinolate and mineral element composition in leaves of Moringa oleifera}, series = {Mycorrhiza}, volume = {24}, journal = {Mycorrhiza}, number = {7}, publisher = {Springer}, address = {New York}, issn = {0940-6360}, doi = {10.1007/s00572-014-0574-7}, pages = {565 -- 570}, year = {2014}, abstract = {Moringa is a mycorrhizal crop cultivated in the tropics and subtropics and appreciated for its nutritive and health-promoting value. As well as improving plant mineral nutrition, arbuscular mycorrhizal fungi (AMF) can affect plant synthesis of compounds bioactive against chronic diseases in humans. Rhizophagus intraradices and Funneliformis mosseae were used in a full factorial experiment to investigate the impact of AMF on the accumulation of glucosinolates, flavonoids, phenolic acids, carotenoids, and mineral elements in moringa leaves. Levels of glucosinolates were enhanced, flavonoids and phenolic acids were not affected, levels of carotenoids (including provitamin A) were species-specifically reduced, and mineral elements were affected differently, with only Cu and Zn being increased by the AMF. This study presents novel results on AMF effects on glucosinolates in leaves and supports conclusions that the impacts of these fungi on microelement concentrations in edible plants are species dependent. The nonspecific positive effects on glucosinolates and the species-specific negative effects on carotenoids encourage research on other AMF species to achieve general benefits on bioactive compounds in moringa.}, language = {en} }