@article{RheinwaltBoersMarwanetal.2016, author = {Rheinwalt, Aljoscha and Boers, Niklas and Marwan, Norbert and Kurths, J{\"u}rgen and Hoffmann, Peter and Gerstengarbe, Friedrich-Wilhelm and Werner, Peter}, title = {Non-linear time series analysis of precipitation events using regional climate networks for Germany}, series = {Climate dynamics : observational, theoretical and computational research on the climate system}, volume = {46}, journal = {Climate dynamics : observational, theoretical and computational research on the climate system}, publisher = {Springer}, address = {New York}, issn = {0930-7575}, doi = {10.1007/s00382-015-2632-z}, pages = {1065 -- 1074}, year = {2016}, abstract = {Synchronous occurrences of heavy rainfall events and the study of their relation in time and space are of large socio-economical relevance, for instance for the agricultural and insurance sectors, but also for the general well-being of the population. In this study, the spatial synchronization structure is analyzed as a regional climate network constructed from precipitation event series. The similarity between event series is determined by the number of synchronous occurrences. We propose a novel standardization of this number that results in synchronization scores which are not biased by the number of events in the respective time series. Additionally, we introduce a new version of the network measure directionality that measures the spatial directionality of weighted links by also taking account of the effects of the spatial embedding of the network. This measure provides an estimate of heavy precipitation isochrones by pointing out directions along which rainfall events synchronize. We propose a climatological interpretation of this measure in terms of propagating fronts or event traces and confirm it for Germany by comparing our results to known atmospheric circulation patterns.}, language = {en} } @article{BurattiThomasRoussosetal.2019, author = {Buratti, Bonnie J. and Thomas, P. C. and Roussos, E. and Howett, Carly and Seiss, Martin and Hendrix, A. R. and Helfenstein, Paul and Brown, R. H. and Clark, R. N. and Denk, Tilmann and Filacchione, Gianrico and Hoffmann, Holger and Jones, Geraint H. and Khawaja, N. and Kollmann, Peter and Krupp, Norbert and Lunine, Jonathan and Momary, T. W. and Paranicas, Christopher and Postberg, Frank and Sachse, Manuel and Spahn, Frank and Spencer, John and Srama, Ralf and Albin, T. and Baines, K. H. and Ciarniello, Mauro and Economou, Thanasis and Hsu, Hsiang-Wen and Kempf, Sascha and Krimigis, Stamatios M. and Mitchell, Donald and Moragas-Klostermeyer, Georg and Nicholson, Philip D. and Porco, C. C. and Rosenberg, Heike and Simolka, Jonas and Soderblom, Laurence A.}, title = {Close Cassini flybys of Saturn's ring moons Pan, Daphnis, Atlas, Pandora, and Epimetheus}, series = {Science}, volume = {364}, journal = {Science}, number = {6445}, publisher = {American Assoc. for the Advancement of Science}, address = {Washington}, issn = {0036-8075}, doi = {10.1126/science.aat2349}, pages = {1053}, year = {2019}, abstract = {Saturn's main ring system is associated with a set of small moons that either are embedded within it or interact with the rings to alter their shape and composition. Five close flybys of the moons Pan, Daphnis, Atlas, Pandora, and Epimetheus were performed between December 2016 and April 2017 during the ring-grazing orbits of the Cassini mission. Data on the moons' morphology, structure, particle environment, and composition were returned, along with images in the ultraviolet and thermal infrared. We find that the optical properties of the moons' surfaces are determined by two competing processes: contamination by a red material formed in Saturn's main ring system and accretion of bright icy particles or water vapor from volcanic plumes originating on the moon Enceladus.}, language = {en} } @book{HoltmannHoltmannGoerletal.2004, author = {Holtmann, Dieter and Holtmann, Elisabeth and G{\"o}rl, Tilo and Goltz, Elke and Fischer, Ulrike and Janeczka, Ines and Jacobi, Lena and Otto, Christian and Klauß, Christian and Hoffmann, Juliane and Tinsner, Karen and Patzwald, Claudia and Buchheister, Claudia and Bsdok, Ursula and Christ, Mirja and Elsner, Anne and Hagenm{\"u}ller, Jan-Peter and Kellner, Andreas}, title = {Gewalt und Fremdenfeindlichkeit : Erkl{\"a}rungsfaktoren sowie Handlungsempfehlungen zu den kriminalpr{\"a}ventiven und zivilgesellschaftlichen Potentialen}, publisher = {Landespr{\"a}ventionsrat Potsdam}, address = {Potsdam}, pages = {76 S.}, year = {2004}, language = {de} } @article{VaidyaSchmidtRakowskietal.2021, author = {Vaidya, Shrijana and Schmidt, Marten and Rakowski, Peter and Bonk, Norbert and Verch, Gernot and Augustin, J{\"u}rgen and Sommer, Michael and Hoffmann, Mathias}, title = {A novel robotic chamber system allowing to accurately and precisely determining spatio-temporal CO2 flux dynamics of heterogeneous croplands}, series = {Agricultural and forest meteorology}, volume = {296}, journal = {Agricultural and forest meteorology}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0168-1923}, doi = {10.1016/j.agrformet.2020.108206}, pages = {9}, year = {2021}, abstract = {The precise and accurate assessment of carbon dioxide (CO2) exchange is crucial to identify terrestrial carbon (C) sources and sinks and for evaluating their role within the global C budget. The substantial uncertainty in disentangling the management and soil impact on measured CO2 fluxes are largely ignored especially in cropland. The reasons for this lies in the limitation of the widely used eddy covariance as well as manual and automatic chamber systems, which either account for short-term temporal variability or small-scale spatial heterogeneity, but barely both. To address this issue, we developed a novel robotic chamber system allowing for dozens of spatial measurement repetitions, thus enabling CO2 exchange measurements in a sufficient temporal and high small-scale spatial resolution. The system was tested from 08th July to 09th September 2019 at a heterogeneous field (100 m x 16 m), located within the hummocky ground moraine landscape of northeastern Germany (CarboZALF-D). The field is foreseen for a longer-term block trial manipulation experiment extending over three erosion induced soil types and was covered with spring barley. Measured fluxes of nighttime ecosystem respiration (R-eco) and daytime net ecosystem exchange (NEE) showed distinct temporal patterns influenced by crop phenology, weather conditions and management practices. Similarly, we found clear small-scale spatial differences in cumulated (gap-filled) R-eco, gross primary productivity (GPP) and NEE fluxes affected by the three distinct soil types. Additionally, spatial patterns induced by former management practices and characterized by differences in soil pH and nutrition status (P and K) were also revealed between plots within each of the three soil types, which allowed compensating for prior to the foreseen block trial manipulation experiment. The results underline the great potential of the novel robotic chamber system, which not only detects short-term temporal CO2 flux dynamics but also reflects the impact of small-scale spatial heterogeneity.}, language = {en} } @article{SharkovskaKalkLawrenzetal.2010, author = {Sharkovska, Yuliya and Kalk, Philipp and Lawrenz, Bettina and Godes, Michael and Hoffmann, Linda Sarah and Wellkisch, Kathrin and Geschka, Sandra and Relle, Katharina and Hocher, Berthold and Stasch, Johannes-Peter}, title = {Nitric oxide-independent stimulation of soluble guanylate cyclase reduces organ damage in experimental low- renin and high-renin models}, issn = {0263-6352}, doi = {10.1097/Hjh.0b013e32833b558c}, year = {2010}, abstract = {Objectives The nitric oxide-soluble guanylate cyclase (sGC)-cGMP signal transduction pathway is impaired in different cardiovascular diseases, including pulmonary hypertension, heart failure and arterial hypertension. Riociguat is a novel stimulator of soluble guanylate cyclase (sGC). However, little is known about the effects of sGC stimulators in experimental models of hypertension. We thus investigated the cardio-renal protective effects of riociguat in low- renin and high-renin rat models of hypertension. Methods The vasorelaxant effect of riociguat was tested in vitro on isolated saphenous artery rings of normal and nitrate tolerant rabbits. The cardiovascular in-vivo effects of sGC stimulation were evaluated in hypertensive renin-transgenic rats treated with the nitric oxide-synthase inhibitor N- nitro-L-arginine methyl ester (L-NAME) (high-renin model) and in rats with 5/6 nephrectomy (low-renin model). Results In both animal models, riociguat treatment improved survival and normalized blood pressure. Moreover, in the L-NAME study part, riociguat reduced cardiac target organ damage as indicated by lower plasma ANP, lower relative left ventricular weight and lower cardiac interstitial fibrosis, and reduced renal target organ damage as indicated by lower plasma creatinine and urea, less glomerulosclerosis and less renal interstitial fibrosis. In the 5/6 nephrectomy study part, riociguat reduced cardiac target organ damage as indicated by lower plasma ANP, lower relative left ventricular weight, lower myocyte diameter and lower arterial media/lumen ratio, and reduced renal target organ damage as indicated by improved creatinine clearance and less renal interstitial fibrosis. Conclusion We demonstrate for the first time that the novel sGC stimulator riociguat shows in two independent models of hypertension a potent protection against cardiac and renal target organ damage. J Hypertens 28: 1666-1675 (c) 2010 Wolters Kluwer Health vertical bar Lippincott Williams \& Wilkins.}, language = {en} } @article{AlbertAuffretCosynsetal.2015, author = {Albert, Aurelie and Auffret, Alistair G. and Cosyns, Eric and Cousins, Sara A. O. and Eichberg, Carsten and Eycott, Amy E. and Heinken, Thilo and Hoffmann, Maurice and Jaroszewicz, Bogdan and Malo, Juan E. and Marell, Anders and Mouissie, Maarten and Pakeman, Robin J. and Picard, Melanie and Plue, Jan and Poschlod, Peter and Provoost, Sam and Schulze, Kiowa Alraune and Baltzinger, Christophe}, title = {Seed dispersal by ungulates as an ecological filter: a trait-based meta-analysis}, series = {Oikos}, volume = {124}, journal = {Oikos}, number = {9}, publisher = {Wiley-Blackwell}, address = {Hoboken}, issn = {0030-1299}, doi = {10.1111/oik.02512}, pages = {1109 -- 1120}, year = {2015}, abstract = {Plant communities are often dispersal-limited and zoochory can be an efficient mechanism for plants to colonize new patches of potentially suitable habitat. We predicted that seed dispersal by ungulates acts as an ecological filter - which differentially affects individuals according to their characteristics and shapes species assemblages - and that the filter varies according to the dispersal mechanism (endozoochory, fur-epizoochory and hoof-epizoochory). We conducted two-step individual participant data meta-analyses of 52 studies on plant dispersal by ungulates in fragmented landscapes, comparing eight plant traits and two habitat indicators between dispersed and non-dispersed plants. We found that ungulates dispersed at least 44\% of the available plant species. Moreover, some plant traits and habitat indicators increased the likelihood for plant of being dispersed. Persistent or nitrophilous plant species from open habitats or bearing dry or elongated diaspores were more likely to be dispersed by ungulates, whatever the dispersal mechanism. In addition, endozoochory was more likely for diaspores bearing elongated appendages whereas epizoochory was more likely for diaspores released relatively high in vegetation. Hoof-epizoochory was more likely for light diaspores without hooked appendages. Fur-epizoochory was more likely for diaspores with appendages, particularly elongated or hooked ones. We thus observed a gradient of filtering effect among the three dispersal mechanisms. Endozoochory had an effect of rather weak intensity (impacting six plant characteristics with variations between ungulate-dispersed and non-dispersed plant species mostly below 25\%), whereas hoof-epizoochory had a stronger effect (eight characteristics included five ones with above 75\% variation), and fur-epizoochory an even stronger one (nine characteristics included six ones with above 75\% variation). Our results demonstrate that seed dispersal by ungulates is an ecological filter whose intensity varies according to the dispersal mechanism considered. Ungulates can thus play a key role in plant community dynamics and have implications for plant spatial distribution patterns at multiple scales.}, language = {en} } @article{StrehmelBerdzinskiStrauchetal.2014, author = {Strehmel, Veronika and Berdzinski, Stefan and Strauch, Peter and Hoffmann-Jacobsen, Kerstin and Strehmel, Bernd}, title = {Investigation of molecular solvents and ionic liquids with a dual probe}, series = {Zeitschrift f{\"u}r physikalische Chemie : international journal of research in physical chemistry and chemical physics}, volume = {228}, journal = {Zeitschrift f{\"u}r physikalische Chemie : international journal of research in physical chemistry and chemical physics}, number = {2-3}, publisher = {De Gruyter}, address = {Berlin}, issn = {0942-9352}, doi = {10.1515/zpch-2014-0453}, pages = {155 -- 169}, year = {2014}, abstract = {A dual probe was investigated by UV-Vis, fluorescence, and ESR spectroscopy. It comprises the pyrene chromophore and the paramagnetic 2,2,6,6-tetramethylpiperidinyl-N-oxyl radical that are covalently linked together via an ester bridge. The dual probe was used to investigate molecular solvents of different polarity as well as ionic liquids bearing either imidazolium or pyrrolidinium cations and various anions, such as bis(trifluoromethylsulfonyl)imide, tetrafluoroborate, tris(pentafluoroethyl)trifluorophosphate, or dicyanamide. The dual probe does not show solvatochromism that is typical for some pyrenes. Furthermore, the dual probe is considerable less mobile compared to 2,2,6,6-tetramethylpiperidinyl-N-oxyl (TEMPO) without additional substituent as detected by ESR spectroscopy. This is caused by the bulky pyrenyl substituent bound at the dual probe resulting in a reduced mobility of the dual probe.}, language = {en} } @misc{HoffmannKretschmerLawrenzetal.2015, author = {Hoffmann, Linda Sarah and Kretschmer, Axel and Lawrenz, Bettina and Hocher, Berthold and Stasch, Johannes-Peter}, title = {Chronic activation of heme free Guanylate Cyclase leads to renal protection in Dahl salt-sensitive rats}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch naturwissenschaftliche Reihe}, number = {489}, issn = {1866-8372}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-408088}, pages = {17}, year = {2015}, abstract = {The nitric oxide (NO)/soluble guanylate cyclase (sGC)/cyclic guanosine monophasphate (cGMP)-signalling pathway is impaired under oxidative stress conditions due to oxidation and subsequent loss of the prosthetic sGC heme group as observed in particular in chronic renal failure. Thus, the pool of heme free sGC is increased under pathological conditions. sGC activators such as cinaciguat selectively activate the heme free form of sGC and target the disease associated enzyme. In this study, a therapeutic effect of long-term activation of heme free sGC by the sGC activator cinaciguat was investigated in an experimental model of salt-sensitive hypertension, a condition that is associated with increased oxidative stress, heme loss from sGC and development of chronic renal failure. For that purpose Dahl/ss rats, which develop severe hypertension upon high salt intake, were fed a high salt diet (8\% NaCl) containing either placebo or cinaciguat for 21 weeks. Cinaciguat markedly improved survival and ameliorated the salt-induced increase in blood pressure upon treatment with cinaciguat compared to placebo. Renal function was significantly improved in the cinaciguat group compared to the placebo group as indicated by a significantly improved glomerular filtration rate and reduced urinary protein excretion. This was due to anti-fibrotic and antiinflammatory effects of the cinaciguat treatment. Taken together, this is the first study showing that long-term activation of heme free sGC leads to renal protection in an experimental model of hypertension and chronic kidney disease. These results underline the promising potential of cinaciguat to treat renal diseases by targeting the disease associated heme free form of sGC.}, language = {en} } @misc{SchatzOhlendorfBusseetal.2013, author = {Schatz, Juliane and Ohlendorf, Bernd and Busse, Peter and Pelz, Gerrit and Dolch, Dietrich and Teubner, Jens and Encarnacao, Jorge A. and M{\"u}hle, Ralf-Udo and Fischer, M. and Hoffmann, Bernd and Kwasnitschka, Linda and Balkema-Buschmann, Anne and Mettenleiter, Thomas Christoph and M{\"u}ller, T. and Freuling, Conrad M.}, title = {Twenty years of active bat rabies surveillance in Germany}, series = {Postprints der Universit{\"a}t Potsdam Humanwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam Humanwissenschaftliche Reihe}, number = {533}, issn = {1866-8364}, doi = {10.25932/publishup-41514}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-415140}, pages = {12}, year = {2013}, abstract = {In Germany, active bat rabies surveillance was conducted between 1993 and 2012. A total of 4546 oropharyngeal swab samples from 18 bat species were screened for the presence of EBLV-1- , EBLV-2- and BBLV-specific RNA. Overall, 0 center dot 15\% of oropharyngeal swab samples tested EBLV-1 positive, with the majority originating from Eptesicus serotinus. Interestingly, out of seven RT-PCR-positive oropharyngeal swabs subjected to virus isolation, viable virus was isolated from a single serotine bat (E. serotinus). Additionally, about 1226 blood samples were tested serologically, and varying virus neutralizing antibody titres were found in at least eight different bat species. The detection of viral RNA and seroconversion in repeatedly sampled serotine bats indicates long-term circulation of the virus in a particular bat colony. The limitations of random-based active bat rabies surveillance over passive bat rabies surveillance and its possible application of targeted approaches for future research activities on bat lyssavirus dynamics and maintenance are discussed.}, language = {en} } @article{WeberAbuAyyashAbueladasetal.2009, author = {Weber, Michael H. and Abu-Ayyash, Khalil and Abueladas, Abdel-Rahman and Agnon, Amotz and Alasonati-Taš{\´a}rov{\´a}, Zuzana and Al-Zubi, Hashim and Babeyko, Andrey and Bartov, Yuval and Bauer, Klaus and Becken, Michael and Bedrosian, Paul A. and Ben-Avraham, Zvi and Bock, G{\"u}nter and Bohnhoff, Marco and Bribach, Jens and Dulski, Peter and Ebbing, Joerg and El-Kelani, Radwan J. and Foerster, Andrea and F{\"o}rster, Hans-J{\"u}rgen and Frieslander, Uri and Garfunkel, Zvi and G{\"o}tze, Hans-J{\"u}rgen and Haak, Volker and Haberland, Christian and Hassouneh, Mohammed and Helwig, Stefan L. and Hofstetter, Alfons and Hoffmann-Rothe, Arne and Jaeckel, Karl-Heinz and Janssen, Christoph and Jaser, Darweesh and Kesten, Dagmar and Khatib, Mohammed Ghiath and Kind, Rainer and Koch, Olaf and Koulakov, Ivan and Laske, Maria Gabi and Maercklin, Nils}, title = {Anatomy of the Dead Sea transform from lithospheric to microscopic scale}, issn = {8755-1209}, doi = {10.1029/2008rg000264}, year = {2009}, abstract = {Fault zones are the locations where motion of tectonic plates, often associated with earthquakes, is accommodated. Despite a rapid increase in the understanding of faults in the last decades, our knowledge of their geometry, petrophysical properties, and controlling processes remains incomplete. The central questions addressed here in our study of the Dead Sea Transform (DST) in the Middle East are as follows: (1) What are the structure and kinematics of a large fault zone? (2) What controls its structure and kinematics? (3) How does the DST compare to other plate boundary fault zones? The DST has accommodated a total of 105 km of left-lateral transform motion between the African and Arabian plates since early Miocene (similar to 20 Ma). The DST segment between the Dead Sea and the Red Sea, called the Arava/Araba Fault (AF), is studied here using a multidisciplinary and multiscale approach from the mu m to the plate tectonic scale. We observe that under the DST a narrow, subvertical zone cuts through crust and lithosphere. First, from west to east the crustal thickness increases smoothly from 26 to 39 km, and a subhorizontal lower crustal reflector is detected east of the AF. Second, several faults exist in the upper crust in a 40 km wide zone centered on the AF, but none have kilometer-size zones of decreased seismic velocities or zones of high electrical conductivities in the upper crust expected for large damage zones. Third, the AF is the main branch of the DST system, even though it has accommodated only a part (up to 60 km) of the overall 105 km of sinistral plate motion. Fourth, the AF acts as a barrier to fluids to a depth of 4 km, and the lithology changes abruptly across it. Fifth, in the top few hundred meters of the AF a locally transpressional regime is observed in a 100-300 m wide zone of deformed and displaced material, bordered by subparallel faults forming a positive flower structure. Other segments of the AF have a transtensional character with small pull-aparts along them. The damage zones of the individual faults are only 5-20 m wide at this depth range. Sixth, two areas on the AF show mesoscale to microscale faulting and veining in limestone sequences with faulting depths between 2 and 5 km. Seventh, fluids in the AF are carried downward into the fault zone. Only a minor fraction of fluids is derived from ascending hydrothermal fluids. However, we found that on the kilometer scale the AF does not act as an important fluid conduit. Most of these findings are corroborated using thermomechanical modeling where shear deformation in the upper crust is localized in one or two major faults; at larger depth, shear deformation occurs in a 20-40 km wide zone with a mechanically weak decoupling zone extending subvertically through the entire lithosphere.}, language = {en} } @article{DippongCarlLenzetal.2017, author = {Dippong, Martin and Carl, Peter and Lenz, Christine and Schenk, J{\"o}rg A. and Hoffmann, Katrin and Schwaar, Timm and Schneider, Rudolf J. and Kuhne, Maren}, title = {Hapten-Specific Single-Cell Selection of Hybridoma Clones by Fluorescence-Activated Cell Sorting for the Generation of Monoclonal Antibodies}, series = {Analytical chemistry}, volume = {89}, journal = {Analytical chemistry}, publisher = {American Chemical Society}, address = {Washington}, issn = {0003-2700}, doi = {10.1021/acs.analchem.6b04569}, pages = {4007 -- 4012}, year = {2017}, language = {en} } @book{AlbersAppelBaueretal.2008, author = {Albers, Marion and Appel, Ivo and Bauer, Hartmut and von Bogdandy, Armin and Britz, Gabriele and Bumke, Wolfgang and Fehling, Michael and Gusy, Christoph and Hermes, Georg and Hill, Hermann and Hoffmann-Riem, Wolfgang and Holznagel, Bernd and K{\"o}ck, Wolfgang and Ladeur, Karl-Heinz and Michael, Lothar and Pitschas, Rainer and R{\"o}hl, Hans Christian and Rossen-Stahlfeld, Helge and Sachs, Michael and Sachsofsky, Ute and Schmidt-Aßmann, Eberhard and Schneider, Jens-Peter and Vesting, Thomas}, title = {Grundlagen des Verwaltungsrechts : Bd. II Informationsordnung, Verwaltungsverfahren, Handlungsformen}, volume = {2}, editor = {Hoffmann-Riem, Wolfgang and Schmidt-Aßmann, Eberhard and Voßkuhle, Andreas}, publisher = {Beck}, address = {M{\"u}nchen}, isbn = {978-3-406-54718-8}, pages = {1712 S.}, year = {2008}, language = {de} } @article{HoffmannOsterlohStoneetal.2012, author = {Hoffmann, Anne and Osterloh, Lukas and Stone, Robert and Lampert, Astrid and Ritter, Christoph and Stock, Maria and Tunved, Peter and Hennig, Tabea and B{\"o}ckmann, Christine and Li, Shao-Meng and Eleftheriadis, Kostas and Maturilli, Marion and Orgis, Thomas and Herber, Andreas and Neuber, Roland and Dethloff, Klaus}, title = {Remote sensing and in-situ measurements of tropospheric aerosol, a PAMARCMiP case study}, series = {Atmospheric environment : air pollution ; emissions, transport and dispersion, transformation, deposition effects, micrometeorology, urban atmosphere, global atmosphere}, volume = {52}, journal = {Atmospheric environment : air pollution ; emissions, transport and dispersion, transformation, deposition effects, micrometeorology, urban atmosphere, global atmosphere}, number = {3}, publisher = {Elsevier}, address = {Oxford}, issn = {1352-2310}, doi = {10.1016/j.atmosenv.2011.11.027}, pages = {56 -- 66}, year = {2012}, abstract = {In this work, a closure experiment for tropospheric aerosol is presented. Aerosol size distributions and single scattering albedo from remote sensing data are compared to those measured in-situ. An aerosol pollution event on 4 April 2009 was observed by ground based and airborne lidar and photometer in and around Ny-Alesund, Spitsbergen, as well as by DMPS, nephelometer and particle soot absorption photometer at the nearby Zeppelin Mountain Research Station. The presented measurements were conducted in an area of 40 x 20 km around Ny-Alesund as part of the 2009 Polar Airborne Measurements and Arctic Regional Climate Model Simulation Project (PAMARCMiP). Aerosol mainly in the accumulation mode was found in the lower troposphere, however, enhanced backscattering was observed up to the tropopause altitude. A comparison of meteorological data available at different locations reveals a stable multi-layer-structure of the lower troposphere. It is followed by the retrieval of optical and microphysical aerosol parameters. Extinction values have been derived using two different methods, and it was found that extinction (especially in the UV) derived from Raman lidar data significantly surpasses the extinction derived from photometer AOD profiles. Airborne lidar data shows volume depolarization values to be less than 2.5\% between 500 m and 2.5 km altitude, hence, particles in this range can be assumed to be of spherical shape. In-situ particle number concentrations measured at the Zeppelin Mountain Research Station at 474 m altitude peak at about 0.18 mu m diameter, which was also found for the microphysical inversion calculations performed at 850 m and 1500 m altitude. Number concentrations depend on the assumed extinction values, and slightly decrease with altitude as well as the effective particle diameter. A low imaginary part in the derived refractive index suggests weakly absorbing aerosols, which is confirmed by low black carbon concentrations, measured at the Zeppelin Mountain as well as on board the Polar 5 aircraft.}, language = {en} } @article{KaneHoffmannBaumgaerteletal.2008, author = {Kane, Avinash S. and Hoffmann, Armin S. and Baumg{\"a}rtel, Peter and Seckler, Robert and Reichardt, Gerd and Horsley, David A. and Schuler, Benjamin and Bakajin, Olgica}, title = {Microfluidic mixers for the investigation of rapid protein folding kinetics using synchrotron radiation circular dichroism spectroscopy}, issn = {0003-2700}, year = {2008}, abstract = {We have developed a microfluidic mixer optimized for rapid measurements of protein folding kinetics using synchrotron radiation circular dichroism (SRCD) spectroscopy. The combination of fabrication in fused silica and synchrotron radiation allows measurements at wavelengths below 220 nm, the typical limit of commercial instrumentation. At these wavelengths, the discrimination between the different types of protein secondary structure increases sharply. The device was optimized for rapid mixing at moderate sample consumption by employing a serpentine channel design, resulting in a dead time of less than 200 ;s. Here, we discuss the design and fabrication of the mixer and quantify the mixing efficiency using wide-field and confocal epi-fluorescence microscopy. We demonstrate the performance of the device in SRCD measurements of the folding kinetics of cytochrome c, a small, fast-folding protein. Our results show that the combination of SRCD with microfluidic mixing opens new possibilities for investigating rapid conformational changes in biological macromolecules that have previously been inaccessible.}, language = {en} } @article{HavermannGhirardoSchnitzleretal.2022, author = {Havermann, Felix and Ghirardo, Andrea and Schnitzler, J{\"o}rg-Peter and Nendel, Claas and Hoffmann, Mathias and Kraus, David and Grote, R{\"u}diger}, title = {Modeling intra- and interannual variability of BVOC emissions from maize, oil-seed rape, and ryegrass}, series = {Journal of advances in modeling earth systems}, volume = {14}, journal = {Journal of advances in modeling earth systems}, number = {3}, publisher = {American Geophysical Union}, address = {Washington}, issn = {1942-2466}, doi = {10.1029/2021MS002683}, pages = {22}, year = {2022}, abstract = {Air chemistry is affected by the emission of biogenic volatile organic compounds (BVOCs), which originate from almost all plants in varying qualities and quantities. They also vary widely among different crops, an aspect that has been largely neglected in emission inventories. In particular, bioenergy-related species can emit mixtures of highly reactive compounds that have received little attention so far. For such species, long-term field observations of BVOC exchange from relevant crops covering different phenological phases are scarcely available. Therefore, we measured and modeled the emission of three prominent European bioenergy crops (maize, ryegrass, and oil-seed rape) for full rotations in north-eastern Germany. Using a proton transfer reaction-mass spectrometer combined with automatically moving large canopy chambers, we were able to quantify the characteristic seasonal BVOC flux dynamics of each crop species. The measured BVOC fluxes were used to parameterize and evaluate the BVOC emission module (JJv) of the physiology-oriented LandscapeDNDC model, which was enhanced to cover de novo emissions as well as those from plant storage pools. Parameters are defined for each compound individually. The model is used for simulating total compound-specific reactivity over several years and also to evaluate the importance of these emissions for air chemistry. We can demonstrate substantial differences between the investigated crops with oil-seed rape having 37-fold higher total annual emissions than maize. However, due to a higher chemical reactivity of the emitted blend in maize, potential impacts on atmospheric OH-chemistry are only 6-fold higher.}, language = {en} } @article{HoffmannKaneNettelsetal.2007, author = {Hoffmann, Armin S. and Kane, Avinash S. and Nettels, Daniel and Hertzog, David E. and Baumg{\"a}rtel, Peter and Lengefeld, Jan and Reichardt, Gerd and Horsley, David A. and Seckler, Robert and Bakajin, Olgica and Schuler, Benjamin}, title = {Mapping protein collapse with single molecule fluorescence and kinetic synchrotron radiation circular dichroism spectroscopy}, issn = {0027-8424}, year = {2007}, language = {en} } @article{PeterWenderingSchlickeiseretal.2022, author = {Peter, Lena and Wendering, D{\´e}sir{\´e}e Jacqueline and Schlickeiser, Stephan and Hoffmann, Henrike and Noster, Rebecca and Wagner, Dimitrios Laurin and Zarrinrad, Ghazaleh and M{\"u}nch, Sandra and Picht, Samira and Schulenberg, Sarah and Moradian, Hanieh and Mashreghi, Mir-Farzin and Klein, Oliver and Gossen, Manfred and Roch, Toralf and Babel, Nina and Reinke, Petra and Volk, Hans-Dieter and Amini, Leila and Schmueck-Henneresse, Michael}, title = {Tacrolimus-resistant SARS-CoV-2-specific T cell products to prevent and treat severe COVID-19 in immunosuppressed patients}, series = {Molecular therapy methods and clinical development}, volume = {25}, journal = {Molecular therapy methods and clinical development}, publisher = {Cell Press}, address = {Cambridge}, issn = {2329-0501}, doi = {10.1016/j.omtm.2022.02.012}, pages = {52 -- 73}, year = {2022}, abstract = {Solid organ transplant (SOT) recipients receive therapeutic immunosuppression that compromises their immune response to infections and vaccines. For this reason, SOT patients have a high risk of developing severe coronavirus disease 2019 (COVID-19) and an increased risk of death from severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Moreover, the efficiency of immunotherapies and vaccines is reduced due to the constant immunosuppression in this patient group. Here, we propose adoptive transfer of SARS-CoV-2-specific T cells made resistant to a common immunosuppressant, tacrolimus, for optimized performance in the immunosuppressed patient. Using a ribonucleoprotein approach of CRISPR-Cas9 technology, we have generated tacrolimus-resistant SARS-CoV-2-specific T cell products from convalescent donors and demonstrate their specificity and function through characterizations at the single-cell level, including flow cytometry, single-cell RNA (scRNA) Cellular Indexing of Transcriptomes and Epitopes (CITE), and T cell receptor (TCR) sequencing analyses. Based on the promising results, we aim for clinical validation of this approach in transplant recipients. Additionally, we propose a combinatory approach with tacrolimus, to prevent an overshooting immune response manifested as bystander T cell activation in the setting of severe COVID-19 immunopathology, and tacrolimus-resistant SARS-CoV-2-specific T cell products, allowing for efficient clearance of viral infection. Our strategy has the potential to prevent severe COVID-19 courses in SOT or autoimmunity settings and to prevent immunopathology while providing viral clearance in severe non-transplant COVID-19 cases.}, language = {en} }