@article{SchulzeAppelhansTierschetal.2014, author = {Schulze, Nicole and Appelhans, D. and Tiersch, Brigitte and Koetz, Joachim}, title = {Morphological transformation of vesicles into tubular structures by adding polyampholytes or dendritic glycopolymers}, series = {Colloids and surfaces : an international journal devoted to the principles and applications of colloid and interface science ; A, Physicochemical and engineering aspects}, volume = {457}, journal = {Colloids and surfaces : an international journal devoted to the principles and applications of colloid and interface science ; A, Physicochemical and engineering aspects}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0927-7757}, doi = {10.1016/j.colsurfa.2014.06.007}, pages = {326 -- 332}, year = {2014}, abstract = {For the first time tubulating properties of spherical dendritic glycopolymers and linear alternating polyampholytes against non-uniform negatively charged giant vesicles are proven by light microscopy and cryo-scanning electron microscopy study. Real time observation of the morphological transformation from giant vesicles to tubular structures, simulating morphogenesis in living cells, is given by using the cationic and H-bond active dendritic glycopolymer accompanied by reducing the size of the giant vesicles and the evidence of vesicle-vesicle interaction which was only postulated in a previous study. Similar morphogenesis of non-uniform giant vesicles into tubular network structure can be observed by using a polyampholyte in the stretched conformation at pH 9. Pearl necklace and tubular network structure formation are also observed by applying anionic vesicles of significant smaller dimensions with average size dimensions of 35 nm, after adding the polyampholyte at pH 9. However, the fitting accuracy between the functional groups along the backbone chain of the polyampholyte on one side and the vesicle surface on the other side is of high importance for the transformation process by using polyampholytes. The resulting tubular and network structures offer new fields of application as microfluidic transport channels or template phases for the shape controlled formation of nanoparticles. (C) 2014 Elsevier B.V. All rights reserved.}, language = {en} } @article{SchulzeTierschZenkeetal.2013, author = {Schulze, Nicole and Tiersch, B. and Zenke, I. and Koetz, Joachim}, title = {Polyampholyte-tuned lyotrop lamellar liquid crystalline systems}, series = {COLLOID AND POLYMER SCIENCE}, volume = {291}, journal = {COLLOID AND POLYMER SCIENCE}, number = {11}, publisher = {SPRINGER}, address = {NEW YORK}, issn = {0303-402X}, doi = {10.1007/s00396-013-2999-5}, pages = {2551 -- 2559}, year = {2013}, abstract = {The influence of a polyampholyte, i.e., poly(N,N\’-diallyl-N,N\’-dimethyl-altmaleamic carboxylate) (PalH), on the lamellar liquid crystalline (LC) system sodium dodecyl sulfate (SDS)/decanol/water was investigated by means of microdifferential scanning calorimetry, small-angle X-ray diffraction (SAXS), and cryo-scanning electron microscopy. After incorporating PalH into the lamellar liquid crystalline system, SAXS measurements show that three different LC phases exist: i.e., a swelling, slightly swelling, and non-swelling one. At pH 4, the positively charged polymer with an extended conformation can directly adsorb at the anionic head groups of the surfactant and more compact vesicles are formed at room temperature. At pH 9, the electrostatic interactions between the polyampholyte (in a more coiled conformation) and the sulfate head groups of the SDS are leveled off and incompact vesicles are formed at room temperature. That means in presence of the polyampholyte the morphology of the LC phase, i.e., the supramolecular vesicle structure, can be tuned by varying the pH and/or the temperature.}, language = {en} } @article{KanzleiterJaehnertSchulzeetal.2015, author = {Kanzleiter, Timo and Jaehnert, Markus and Schulze, Gunnar and Selbig, Joachim and Hallahan, Nicole and Schwenk, Robert Wolfgang and Sch{\"u}rmann, Annette}, title = {Exercise training alters DNA methylation patterns in genes related to muscle growth and differentiation in mice}, series = {American journal of physiology : Endocrinology and metabolism}, volume = {308}, journal = {American journal of physiology : Endocrinology and metabolism}, number = {10}, publisher = {American Chemical Society}, address = {Bethesda}, issn = {0193-1849}, doi = {10.1152/ajpendo.00289.2014}, pages = {E912 -- E920}, year = {2015}, abstract = {The adaptive response of skeletal muscle to exercise training is tightly controlled and therefore requires transcriptional regulation. DNA methylation is an epigenetic mechanism known to modulate gene expression, but its contribution to exercise-induced adaptations in skeletal muscle is not well studied. Here, we describe a genome-wide analysis of DNA methylation in muscle of trained mice (n = 3). Compared with sedentary controls, 2,762 genes exhibited differentially methylated CpGs (P < 0.05, meth diff >5\%, coverage > 10) in their putative promoter regions. Alignment with gene expression data (n = 6) revealed 200 genes with a negative correlation between methylation and expression changes in response to exercise training. The majority of these genes were related to muscle growth and differentiation, and a minor fraction involved in metabolic regulation. Among the candidates were genes that regulate the expression of myogenic regulatory factors (Plexin A2) as well as genes that participate in muscle hypertrophy (Igfbp4) and motor neuron innervation (Dok7). Interestingly, a transcription factor binding site enrichment study discovered significantly enriched occurrence of CpG methylation in the binding sites of the myogenic regulatory factors MyoD and myogenin. These findings suggest that DNA methylation is involved in the regulation of muscle adaptation to regular exercise training.}, language = {en} } @phdthesis{Schulze2017, author = {Schulze, Nicole}, title = {Neue Templatphasen zur anisotropen Goldnanopartikelherstellung durch den Einsatz strukturbildender Polymere}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-409515}, school = {Universit{\"a}t Potsdam}, pages = {VI, 117, xv}, year = {2017}, abstract = {Ziel der vorliegenden Arbeit war die Synthese und Charakterisierung von anisotropen Goldnanopartikeln in einer geeigneten Polyelektrolyt-modifizierten Templatphase. Der Mittelpunkt bildet dabei die Auswahl einer geeigneten Templatphase, zur Synthese von einheitlichen und reproduzierbaren anisotropen Goldnanopartikeln mit den daraus resultierenden besonderen Eigenschaften. Bei der Synthese der anisotropen Goldnanopartikeln lag der Fokus in der Verwendung von Vesikeln als Templatphase, wobei hier der Einfluss unterschiedlicher strukturbildender Polymere (stark alternierende Maleamid-Copolymere PalH, PalPh, PalPhCarb und PalPhBisCarb mit verschiedener Konformation) und Tenside (SDS, AOT - anionische Tenside) bei verschiedenen Synthese- und Abtrennungsbedingungen untersucht werden sollte. Im ersten Teil der Arbeit konnte gezeigt werden, dass PalPhBisCarb bei einem pH-Wert von 9 die Bedingungen eines R{\"o}hrenbildners f{\"u}r eine morphologische Transformation von einer vesikul{\"a}ren Phase in eine r{\"o}hrenf{\"o}rmige Netzwerkstruktur erf{\"u}llt und somit als Templatphase zur formgesteuerten Bildung von Nanopartikeln genutzt werden kann. Im zweiten Teil der Arbeit wurde dargelegt, dass die Templatphase PalPhBisCarb (pH-Wert von 9, Konzentration von 0,01 wt.\%) mit AOT als Tensid und PL90G als Phospholipid (im Verh{\"a}ltnis 1:1) die effektivste Wahl einer Templatphase f{\"u}r die Bildung von anisotropen Strukturen in einem einstufigen Prozess darstellt. Bei einer konstanten Synthesetemperatur von 45 °C wurden die besten Ergebnisse bei einer Goldchloridkonzentration von 2 mM, einem Gold-Templat-Verh{\"a}ltnis von 3:1 und einer Synthesezeit von 30 Minuten erzielt. Ausbeute an anisotropen Strukturen lag bei 52 \% (Anteil an dreieckigen Nanopl{\"a}ttchen von 19 \%). Durch Erh{\"o}hung der Synthesetemperatur konnte die Ausbeute auf 56 \% (29 \%) erh{\"o}ht werden. Im dritten Teil konnte durch zeitabh{\"a}ngige Untersuchungen gezeigt werden, dass bei Vorhandensein von PalPhBisCarb die Bildung der energetisch nicht bevorzugten Pl{\"a}ttchen-Strukturen bei Raumtemperatur initiiert wird und bei 45 °C ein Optimum annimmt. Kintetische Untersuchungen haben gezeigt, dass die Bildung dreieckiger Nanopl{\"a}ttchen bei schrittweiser Zugabe der Goldchlorid-Pr{\"a}kursorl{\"o}sung zur PalPhBisCarb enthaltenden Templatphase durch die Dosierrate der vesikul{\"a}ren Templatphase gesteuert werden kann. In umgekehrter Weise findet bei Zugabe der Templatphase zur Goldchlorid-Pr{\"a}kursorl{\"o}sung bei 45 °C ein {\"a}hnlicher, kinetisch gesteuerter Prozess der Bildung von Nanodreiecken statt mit einer maximalen Ausbeute dreieckigen Nanopl{\"a}ttchen von 29 \%. Im letzten Kapitel erfolgten erste Versuche zur Abtrennung dreieckiger Nanopl{\"a}ttchen von den {\"u}brigen Geometrien der gemischten Nanopartikell{\"o}sung mittels tensidinduzierter Verarmungsf{\"a}llung. Bei Verwendung von AOT mit einer Konzentration von 0,015 M wurde eine Ausbeute an Nanopl{\"a}ttchen von 99 \%, wovon 72 \% dreieckiger Geometrien hatten, erreicht.}, language = {de} } @article{SchulzePrietzelKoetz2016, author = {Schulze, Nicole and Prietzel, Claudia Christina and Koetz, Joachim}, title = {Polyampholyte-mediated synthesis of anisotropic gold nanoplatelets}, series = {Colloid and polymer science : official journal of the Kolloid-Gesellschaft}, volume = {294}, journal = {Colloid and polymer science : official journal of the Kolloid-Gesellschaft}, publisher = {Springer}, address = {New York}, issn = {0303-402X}, doi = {10.1007/s00396-016-3890-y}, pages = {1297 -- 1304}, year = {2016}, abstract = {This paper focused on the synthesis of triangular nanoplatelets in the presence of a tubular network structure. The tubular network structure is formed by adding a strongly alternating polyampholyte, i.e., PalPhBisCarb, to a mixed vesicle system with a negatively charged bilayer containing phosphatidylcholin and AOT. Using the tubular network as a reducing agent in a one-step procedure, triangular and hexagonal nanoplatelets are formed. One can show that the nanoplatelet yield is enhanced by increasing the temperature and decreasing the reaction time. The platelet edge length can be decreased by heating the system up to 100 A degrees C. Due to specific interactions between PalPhBisCarb and the AOT/phospholipid bilayer, stacking and welding effects lead to the formation of ordered platelet structures. The reaction pathway to flat gold nanotriangles is discussed with regard to the twin plane growth model of gold nanoplates.}, language = {en} } @article{AgaBarfknechtHallahanGottmannetal.2020, author = {Aga-Barfknecht, Heja and Hallahan, Nicole and Gottmann, Pascal and J{\"a}hnert, Markus and Osburg, Sophie and Schulze, Gunnar and Kamitz, Anne and Arends, Danny and Brockmann, Gudrun and Schallschmidt, Tanja and Lebek, Sandra and Chadt, Alexandra and Al-Hasani, Hadi and Joost, Hans-Georg and Sch{\"u}rmann, Annette and Vogel, Heike}, title = {Identification of novel potential type 2 diabetes genes mediating beta-cell loss and hyperglycemia using positional cloning}, series = {Frontiers in genetics}, volume = {11}, journal = {Frontiers in genetics}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {1664-8021}, doi = {10.3389/fgene.2020.567191}, pages = {11}, year = {2020}, abstract = {Type 2 diabetes (T2D) is a complex metabolic disease regulated by an interaction of genetic predisposition and environmental factors. To understand the genetic contribution in the development of diabetes, mice varying in their disease susceptibility were crossed with the obese and diabetes-prone New Zealand obese (NZO) mouse. Subsequent whole-genome sequence scans revealed one major quantitative trait loci (QTL),Nidd/DBAon chromosome 4, linked to elevated blood glucose and reduced plasma insulin and low levels of pancreatic insulin. Phenotypical characterization of congenic mice carrying 13.6 Mbp of the critical fragment of DBA mice displayed severe hyperglycemia and impaired glucose clearance at week 10, decreased glucose response in week 13, and loss of beta-cells and pancreatic insulin in week 16. To identify the responsible gene variant(s), further congenic mice were generated and phenotyped, which resulted in a fragment of 3.3 Mbp that was sufficient to induce hyperglycemia. By combining transcriptome analysis and haplotype mapping, the number of putative responsible variant(s) was narrowed from initial 284 to 18 genes, including gene models and non-coding RNAs. Consideration of haplotype blocks reduced the number of candidate genes to four (Kti12,Osbpl9,Ttc39a, andCalr4) as potential T2D candidates as they display a differential expression in pancreatic islets and/or sequence variation. In conclusion, the integration of comparative analysis of multiple inbred populations such as haplotype mapping, transcriptomics, and sequence data substantially improved the mapping resolution of the diabetes QTLNidd/DBA. Future studies are necessary to understand the exact role of the different candidates in beta-cell function and their contribution in maintaining glycemic control.}, language = {en} } @article{KramerBouriaudFeindtetal.2022, author = {Kramer, Koen and Bouriaud, Laura and Feindt, Peter H. and van Wassenaer, Lan and Glanemann, Nicole and Hanewinkel, Marc and van der Heide, Martijn and Hengeveld, Geerten M. and Hoogstra, Marjanke and Ingram, Verina and Levermann, Anders and Lindner, Marcus and M{\´a}ty{\´a}s, Csaba and Mohren, Frits and Muys, Bart and Nabuurs, Gert-Jan and Palahi, Marc and Polman, Nico and Reyer, Christopher P. O. and Schulze, Ernst-Detlef and Seidl, Rupert and de Vries, Wim and Werners, Saskia E. and Winkel, Georg and Yousefpour, Rasoul}, title = {Perspective Roadmap to develop a stress test for forest ecosystem services supply}, series = {One Earth}, volume = {5}, journal = {One Earth}, number = {1}, publisher = {Elsevier}, address = {Amsterdam}, issn = {2590-3330}, doi = {10.1016/j.oneear.2021.12.009}, pages = {25 -- 34}, year = {2022}, abstract = {Forests play a key role in a bio-based economy by providing renewable materials, mitigating climate change, and accommodating biodiversity. However, forests experience massive increases in stresses in their ecological and socioeconomic environments, threatening forest ecosystem services supply. Alleviating those stresses is hampered by conflicting and disconnected governance arrangements, competing interests and claims, and rapid changes in technology and social demands. Identifying which stresses threaten forest ecosystem services supply and which factors hamper their alleviation requires stakeholders' perceptions. Stakeholder-oriented stress tests for the supply of forest ecosystem services are therefore necessary but are not yet available. This perspective presents a roadmap to develop a stress test tailored to multiple stakeholders' needs and demands across spatial scales. We provide the Cascade and Resilience Rosetta, with accompanying performance- and resilience indicators, as tools to facilitate development of the stress test. The application of the stress test will facilitate the transition toward a bio-based economy in which healthy and diverse forests provide sustainable and resilient ecosystem services.}, language = {en} } @misc{SchulzeKoetz2016, author = {Schulze, Nicole and Koetz, Joachim}, title = {Kinetically Controlled Growth of Gold Nanotriangles in a Vesicular Template Phase by Adding a Strongly Alternating Polyampholyte}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-98380}, pages = {22}, year = {2016}, abstract = {This paper is focused on the temperature dependent synthesis of gold nanotriangles in a vesicular template phase, containing phosphatidylcholin and AOT, by adding the strongly alternating polyampholyte PalPhBisCarb. UV-vis absorption spectra in combination with TEM micrographs show that flat gold nanoplatelets are formed predominantly in presence of the polyampholyte at 45 °C. The formation of triangular and hexagonal nanoplatelets can be directly influenced by the kinetic approach, i.e., by varying the polyampholyte dosage rate at 45 °C. Corresponding zeta potential measurements indicate that a temperature dependent adsorption of the polyampholyte on the {111} faces will induce the symmetry breaking effect, which is responsible for the kinetically controlled hindered vertical and preferred lateral growth of the nanoplatelets.}, language = {en} } @article{SchulzeKoetz2017, author = {Schulze, Nicole and Koetz, Joachim}, title = {Kinetically controlled growth of gold nanotriangles in a vesicular template phase by adding a strongly alternating polyampholyte}, series = {Journal of dispersion science and technology}, volume = {38}, journal = {Journal of dispersion science and technology}, number = {8}, publisher = {Taylor \& Francis}, address = {Philadelphia}, issn = {0193-2691}, doi = {10.1080/01932691.2016.1220318}, pages = {1073 -- 1078}, year = {2017}, abstract = {This paper is focused on the temperature-dependent synthesis of gold nanotriangles in a vesicular template phase, containing phosphatidylcholine and AOT, by adding the strongly alternating polyampholyte PalPhBisCarb. UV-vis absorption spectra in combination with TEM micrographs show that flat gold nanoplatelets are formed predominantly in the presence of the polyampholyte at 45°C. The formation of triangular and hexagonal nanoplatelets can be directly influenced by the kinetic approach, i.e., by varying the polyampholyte dosage rate at 45°C. Corresponding zeta potential measurements indicate that a temperature-dependent adsorption of the polyampholyte on the {111} faces will induce the symmetry breaking effect, which is responsible for the kinetically controlled hindered vertical and preferred lateral growth of the nanoplatelets.}, language = {en} } @article{KrokeSchmidtAminietal.2022, author = {Kroke, Anja and Schmidt, Annemarie and Amini, Anna M. and Kalotai, Nicole and Lehmann, Andreas and Haardt, Julia and Bauer, J{\"u}rgen M. and Bischoff-Ferrari, Heike A. and Boeing, Heiner and Egert, Sarah and Ellinger, Sabine and K{\"u}hn, Tilman and Louis, Sandrine and Lorkowski, Stefan and Nimptsch, Katharina and Remer, Thomas and Schulze, Matthias B. and Siener, Roswitha and Stangl, Gabriele and Volkert, Dorothee and Zittermann, Armin and Buyken, Anette E. and Watzl, Bernhard and Schwingshackl, Lukas}, title = {Dietary protein intake and health-related outcomes: a methodological protocol for the evidence evaluation and the outline of an evidence to decision framework underlying the evidence-based guideline of the German Nutrition Society}, series = {European journal of nutrition}, volume = {61}, journal = {European journal of nutrition}, number = {4}, publisher = {Springer Nature}, address = {Heidelberg}, organization = {German Nutr Soc}, issn = {1436-6207}, doi = {10.1007/s00394-021-02789-5}, pages = {2091 -- 2101}, year = {2022}, abstract = {Purpose: The present work aimed to delineate (i) a revised protocol according to recent methodological developments in evidence generation, to (ii) describe its interpretation, the assessment of the overall certainty of evidence and to (iii) outline an Evidence to Decision framework for deriving an evidence-based guideline on quantitative and qualitative aspects of dietary protein intake. Methods A methodological protocol to systematically investigate the association between dietary protein intake and several health outcomes and for deriving dietary protein intake recommendations for the primary prevention of various non-communicable diseases in the general adult population was developed. Results The developed methodological protocol relies on umbrella reviews including systematic reviews with or without meta-analyses. Systematic literature searches in three databases will be performed for each health-related outcome. The methodological quality of all selected systematic reviews will be evaluated using a modified version of AMSTAR 2, and the outcome-specific certainty of evidence for systematic reviews with or without meta-analysis will be assessed with NutriGrade. The general outline of the Evidence to Decision framework foresees that recommendations in the derived guideline will be given based on the overall certainty of evidence as well as on additional criteria such as sustainability. Conclusion The methodological protocol permits a systematic evaluation of published systematic reviews on dietary protein intake and its association with selected health-related outcomes. An Evidence to Decision framework will be the basis for the overall conclusions and the resulting recommendations for dietary protein intake.}, language = {en} }