@misc{AichertStaigerSchulteMaeteretal.2010,
  author    = {Aichert, Ingrid and Staiger, Anja and Schulte-M{\"a}ter, Anne and Becker-Redding, Ulrike and Stahn, Corinna and Peschke, Claudia and Heide, Judith and Ott, Susan and Herrmann, Heike and V{\"o}lsch, Juliane and Mayer, J{\"o}rg and Rohnke, Lucie and Frank, Ulrike and Stadie, Nicole and Jentsch, Nadine and Blech, Anke and Kurtenbach, Stephanie and Thieke, Johanna and Schr{\"o}der, Astrid and Stahn, Corinna and H{\"o}rnig, Robin and Burchert, Frank and De Bleser, Ria and Heister, Julian and Bartels, Luise and W{\"u}rzner, Kay-Michael and B{\"o}hme, Romy and Burmester, Juliane and Krajewski, Melanie and Nager, Wido and Jungeh{\"u}lsing, Gerhard Jan and Wartenburger, Isabell and J{\"o}bges, Michael and Schwilling, Eleonore and Lidzba, Karen and Winkler, Susanne and Konietzko, Andreas and Kr{\"a}geloh-Mann, Ingeborg and Rilling, Eva and Wilken, Rainer and Wismann, Kathrin and Glandorf, Birte and Hoffmann, Hannah and Hinnenkamp, Christiane and Rohlmann, Insa and Ludewigt, Jacqueline and Bittner, Christian and Orlov, Tatjana and Claus, Katrin and Ehemann, Christine and Winnecken, Andreas and Hummel, Katja and Breitenstein, Sarah},
  title     = {Spektrum Patholinguistik = Schwerpunktthema: Von der Programmierung zur Artikulation : Sprechapraxie bei Kindern und Erwachsenen},
  number    = {3},
  editor    = {Wahl, Michael and Stahn, Corinna and Hanne, Sandra and Fritzsche, Tom},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  organization    = {Verband f{\"u}r Patholinguistik e. V. (vpl)},
  isbn      = {978-3-86956-079-3},
  issn      = {1869-3822},
  doi       = {10.25932/publishup-4578},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-45470},
  year      = {2010},
  abstract  = {Das 3. Herbsttreffen Patholinguistik fand am 21. November 2009 an der Universit{\"a}t Potsdam statt. Der vorliegende Tagungsband enth{\"a}lt die drei Hauptvortr{\"a}ge zum Schwerpunktthema „Von der Programmierung zu Artikulation: Sprechapraxie bei Kindern und Erwachsenen". Dar{\"u}ber hinaus enth{\"a}lt der Band die Beitr{\"a}ge aus dem Spektrum Patholinguistik, sowie die Abstracts der Posterpr{\"a}sentationen.},
  language  = {de}
}
@phdthesis{Breitenstein2012,
  author    = {Breitenstein, Michael},
  title     = {Ortsaufgel{\"o}ster Aufbau von DNA-Nanostrukturen auf Glasoberfl{\"a}chen},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-61857},
  school      = {Universit{\"a}t Potsdam},
  year      = {2012},
  abstract  = {Im Fokus dieser Arbeit stand der Aufbau einer auf DNA basierenden Nanostruktur. Der universelle Vier-Buchstaben-Code der DNA erm{\"o}glicht es, Bindungen auf molekularer Ebene zu adressieren. Die chemischen und physikalischen Eigenschaften der DNA pr{\"a}destinieren dieses Makromolek{\"u}l f{\"u}r den Einsatz und die Verwendung als Konstruktionselement zum Aufbau von Nanostrukturen. Das Ziel dieser Arbeit war das Aufspannen eines DNA-Stranges zwischen zwei Fixpunkten. Hierf{\"u}r war es notwendig, eine Methode zu entwickeln, welche es erm{\"o}glicht, Funktionsmolek{\"u}le als Ankerelemente ortsaufgel{\"o}st auf eine Oberfl{\"a}che zu deponieren. Das Deponieren dieser Molek{\"u}le sollte dabei im unteren Mikrometermaßstab erfolgen, um den Abmaßen der DNA und der angestrebten Nanostruktur gerecht zu werden. Das eigens f{\"u}r diese Aufgabe entwickelte Verfahren zum ortsaufgel{\"o}sten Deponieren von Funktionsmolek{\"u}len nutzt das Bindungspaar Biotin-Neutravidin. Mit Hilfe eines Rasterkraftmikroskops (AFM) wurde eine zu einem „Stift" umfunktionierte Rasterkraftmikroskopspitze so mit der zu deponierenden „Tinte" beladen, dass das Absetzen von Neutravidin im unteren Mikrometermaßstab m{\"o}glich war. Dieses Neutravidinmolek{\"u}l {\"u}bernahm die Funktion als Bindeglied zwischen der biotinylierten Glasoberfl{\"a}che und dem eigentlichen Adressmolek{\"u}l. Das somit generierte Neutravidin-Feld konnte dann mit einem biotinylierten Adressmolek{\"u}l durch Inkubation funktionalisiert werden. Namensgebend f{\"u}r dieses Verfahren war die M{\"o}glichkeit, Neutravidin mehrmals zu deponieren und zu adressieren. Somit ließ sich sequenziell ein Mehrkomponenten-Feld aufbauen. Die Einschr{\"a}nkung, mit einem AFM nur eine Substanz deponieren zu k{\"o}nnen, wurde so umgangen. Ferner mußten Ankerelemente geschaffen werden, um die DNA an definierten Punkten immobilisieren zu k{\"o}nnen. Die Bearbeitung der DNA erfolgte mit molekularbiologischen Methoden und zielte darauf ab, einen DNA-Strang zu generieren, welcher an seinen beiden Enden komplement{\"a}re Adressequenzen enth{\"a}lt, um gezielt mit den oberfl{\"a}chenst{\"a}ndigen Ankerelementen binden zu k{\"o}nnen. Entsprechend der Geometrie der mit dem AFM erzeugten Fixpunkte und den oligonukleotidvermittelten Adressen kommt es zur Ausbildung einer definierten DNA-Struktur. Mit Hilfe von fluoreszenzmikroskopischen Methoden wurde die aufgebaute DNA-Nanostruktur nachgewiesen. Der Nachweis der nanoskaligen Interaktion von DNA-bindenden Molek{\"u}len mit der generierten DNA-Struktur wurde durch die Bindung von PNA (peptide nucleic acid) an den DNA-Doppelstrang erbracht. Diese PNA-Bindung stellt ihrerseits ein funktionales Strukturelement im Nanometermaßstab dar und wird als Nanostrukturbaustein verstanden.},
  language  = {de}
}
@misc{SchroederGrimmSchulzetal.2017,
  author    = {Schr{\"o}der, Astrid and Grimm, Angela and Schulz, Petra and Heide, Judith and Frank, Ulrike and Wahl, Michael and Lampe, Leonie and Fieder, Nora and Krajenbrink, Trudy and Nickels, Lyndsey and Bykova, Ksenia and Wilzek, Alexa and van den Engl-Hoek, Lenie and Huckabee, Maggie-Lee and Balzer, Julia and Ebert, Susanne and Kaps, Hella and Matteschk, Maria and Tzsch{\"o}ckel, Katharina Andrea and Dressel, Katharina and Kr{\"o}ger, Bernd J. and Diwoky, Laura Cassandra and Breitenstein, Sarah and Bruno, Giulia and Lassotta, Romy and Adani, Flavia and Ferchland, Lisa and Baatz, Charlotte and Netzebandt, Jonka and Heyde, Cornelia J. and Cleland, Joanne and Scobbie, James M. and Roxburgh, Zoe and Schmidt, Jessica},
  title     = {Spektrum Patholinguistik Band 10. Schwerpunktthema: Panorama Patholinguistik: Sprachwissenschaft trifft Sprachtherapie},
  number    = {10},
  editor    = {Adelt, Anne and Yetim, {\"O}zlem and Otto, Constanze and Fritzsche, Tom},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  organization    = {Verband f{\"u}r Patholinguistik e. V. (vpl)},
  isbn      = {978-3-86956-404-3},
  issn      = {1866-9085},
  doi       = {10.25932/publishup-39701},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-397019},
  pages     = {vii, 225 S.},
  year      = {2017},
  abstract  = {Das 10. Herbsttreffen Patholinguistik mit dem Schwerpunktthema »Panorama Patholinguistik: Sprachwissenschaft trifft Sprachtherapie« fand am 19.11.2016 in Potsdam statt. Das Herbsttreffen wird seit 2007 j{\"a}hrlich vom Verband f{\"u}r Patholinguistik e.V. (vpl) durchgef{\"u}hrt. Der vorliegende Tagungsband beinhaltet die vier Hauptvortr{\"a}ge zum Schwerpunktthema sowie Beitr{\"a}ge zu den Kurzvortr{\"a}gen »Patholinguistik im Fokus« und der Posterpr{\"a}sentationen zu weiteren Themen aus der sprachtherapeutischen Forschung und Praxis.},
  language  = {de}
}
@article{BreitensteinNielsenHoelzeletal.2011,
  author    = {Breitenstein, Michael and Nielsen, Peter E. and H{\"o}lzel, Ralph and Bier, Frank Fabian},
  title     = {DNA-nanostructure-assembly by sequential spotting},
  series = {Journal of nanobiotechnology},
  volume    = {9},
  journal   = {Journal of nanobiotechnology},
  number    = {11},
  publisher = {BioMed Central},
  address   = {London},
  issn      = {1477-3155},
  doi       = {10.1186/1477-3155-9-54},
  pages     = {10},
  year      = {2011},
  abstract  = {Background: The ability to create nanostructures with biomolecules is one of the key elements in nanobiotechnology. One of the problems is the expensive and mostly custom made equipment which is needed for their development. We intended to reduce material costs and aimed at miniaturization of the necessary tools that are essential for nanofabrication. Thus we combined the capabilities of molecular ink lithography with DNA-self-assembling capabilities to arrange DNA in an independent array which allows addressing molecules in nanoscale dimensions. Results: For the construction of DNA based nanostructures a method is presented that allows an arrangement of DNA strands in such a way that they can form a grid that only depends on the spotted pattern of the anchor molecules. An atomic force microscope (AFM) has been used for molecular ink lithography to generate small spots. The sequential spotting process allows the immobilization of several different functional biomolecules with a single AFM-tip. This grid which delivers specific addresses for the prepared DNA-strand serves as a two-dimensional anchor to arrange the sequence according to the pattern. Once the DNA-nanoarray has been formed, it can be functionalized by PNA (peptide nucleic acid) to incorporate advanced structures. Conclusions: The production of DNA-nanoarrays is a promising task for nanobiotechnology. The described method allows convenient and low cost preparation of nanoarrays. PNA can be used for complex functionalization purposes as well as a structural element.},
  language  = {en}
}
@misc{BreitensteinNielsenHoelzeletal.2011,
  author    = {Breitenstein, Michael and Nielsen, Peter E. and H{\"o}lzel, Ralph and Bier, Frank Fabian},
  title     = {DNA-nanostructure-assembly by sequential spotting},
  series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  journal   = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  number    = {1027},
  issn      = {1866-8372},
  doi       = {10.25932/publishup-43110},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-431108},
  pages     = {12},
  year      = {2011},
  abstract  = {Background: The ability to create nanostructures with biomolecules is one of the key elements in nanobiotechnology. One of the problems is the expensive and mostly custom made equipment which is needed for their development. We intended to reduce material costs and aimed at miniaturization of the necessary tools that are essential for nanofabrication. Thus we combined the capabilities of molecular ink lithography with DNA-self-assembling capabilities to arrange DNA in an independent array which allows addressing molecules in nanoscale dimensions. Results: For the construction of DNA based nanostructures a method is presented that allows an arrangement of DNA strands in such a way that they can form a grid that only depends on the spotted pattern of the anchor molecules. An atomic force microscope (AFM) has been used for molecular ink lithography to generate small spots. The sequential spotting process allows the immobilization of several different functional biomolecules with a single AFM-tip. This grid which delivers specific addresses for the prepared DNA-strand serves as a two-dimensional anchor to arrange the sequence according to the pattern. Once the DNA-nanoarray has been formed, it can be functionalized by PNA (peptide nucleic acid) to incorporate advanced structures. Conclusions: The production of DNA-nanoarrays is a promising task for nanobiotechnology. The described method allows convenient and low cost preparation of nanoarrays. PNA can be used for complex functionalization purposes as well as a structural element.},
  language  = {en}
}
@misc{BreitensteinHoelzelBier2010,
  author    = {Breitenstein, Michael and H{\"o}lzel, Ralph and Bier, Frank Fabian},
  title     = {Immobilization of different biomolecules by atomic force microscopy},
  series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe},
  journal   = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe},
  number    = {872},
  issn      = {1866-8372},
  doi       = {10.25932/publishup-43507},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-435075},
  pages     = {9},
  year      = {2010},
  abstract  = {Background Micrometer resolution placement and immobilization of probe molecules is an important step in the preparation of biochips and a wide range of lab-on-chip systems. Most known methods for such a deposition of several different substances are costly and only suitable for a limited number of probes. In this article we present a flexible procedure for simultaneous spatially controlled immobilization of functional biomolecules by molecular ink lithography. Results For the bottom-up fabrication of surface bound nanostructures a universal method is presented that allows the immobilization of different types of biomolecules with micrometer resolution. A supporting surface is biotinylated and streptavidin molecules are deposited with an AFM (atomic force microscope) tip at distinct positions. Subsequent incubation with a biotinylated molecule species leads to binding only at these positions. After washing streptavidin is deposited a second time with the same AFM tip and then a second biotinylated molecule species is coupled by incubation. This procedure can be repeated several times. Here we show how to immobilize different types of biomolecules in an arbitrary arrangement whereas most common methods can deposit only one type of molecules. The presented method works on transparent as well as on opaque substrates. The spatial resolution is better than 400 nm and is limited only by the AFM's positional accuracy after repeated z-cycles since all steps are performed in situ without moving the supporting surface. The principle is demonstrated by hybridization to different immobilized DNA oligomers and was validated by fluorescence microscopy. Conclusions The immobilization of different types of biomolecules in high-density microarrays is a challenging task for biotechnology. The method presented here not only allows for the deposition of DNA at submicrometer resolution but also for proteins and other molecules of biological relevance that can be coupled to biotin.},
  language  = {en}
}
@misc{AktasSuccowGieletal.2015,
  author    = {Aktas, Maren and Succow, Juliane and Giel, Barbara and Dressel, Katharina and Lange, Inga and Hanne, Sandra and Burchert, Frank and Vasishth, Shravan and Schwytay, Jeannine and Breitenstein, Sarah and Fleischhauer, Elisabeth and Baumann, Jeannine and Preisinger, Irmhild and Siegm{\"u}ller, Julia and Kuschmann, Anja and Ebert, Susanne and Lowit, Anja and Rath, Elisa and Heide, Judith and Lorenz, Antje and Wartenburger, Isabell and Hippeli, Carolin and Rausch, Monika and W{\"u}rzner, Kay-Michael and Schroeder, Sascha and Czapka, Sophia and Klassert, Annegret and Reuters, Sabine and Frank, Ulrike and Frank, Katrin and Zimmermann, Heinrich and Peiffers, Sabine and Thonicke, Mady},
  title     = {Spektrum Patholinguistik = Schwerpunktthema: Besonders behandeln? : Sprachtherapie im Rahmen prim{\"a}rer St{\"o}rungsbilder},
  number    = {8},
  editor    = {Adelt, Anne and Otto, Constanze and Fritzsche, Tom and Magister, Caroline},
  publisher = {Universit{\"a}tsverlag Potsdam},
  address   = {Potsdam},
  organization    = {Verband f{\"u}r Patholinguistik e. V. (vpl)},
  isbn      = {978-3-86956-335-0},
  issn      = {1869-3822},
  doi       = {10.25932/publishup-7714},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-77147},
  pages     = {vii, 247},
  year      = {2015},
  abstract  = {Das 8. Herbsttreffen Patholinguistik mit dem Schwerpunktthema "Besonders behandeln? Sprachtherapie im Rahmen prim{\"a}rer St{\"o}rungsbilder" fand am 15.11.2014 in Potsdam statt. Das Herbsttreffen wird seit 2007 j{\"a}hrlich vom Verband f{\"u}r Patholinguistik e.V. (vpl) durchgef{\"u}hrt. Der vorliegende Tagungsband beinhaltet die vier Hauptvortr{\"a}ge zum Schwerpunktthema, die vier Kurzvortr{\"a}ge aus dem Spektrum Patholinguisitk sowie die Beitr{\"a}ge der Posterpr{\"a}sentationen zu weiteren Themen aus der sprachtherapeutischen Forschung und Praxis.},
  language  = {de}
}