@article{HiltGrossartMcGinnisetal.2022, author = {Hilt, Sabine and Grossart, Hans-Peter and McGinnis, Daniel F. and Keppler, Frank}, title = {Potential role of submerged macrophytes for oxic methane production in aquatic ecosystems}, series = {Limnology and oceanography}, journal = {Limnology and oceanography}, publisher = {Wiley}, address = {Hoboken}, issn = {0024-3590}, doi = {10.1002/lno.12095}, pages = {13}, year = {2022}, abstract = {Methane (CH4) from aquatic ecosystems contributes to about half of total global CH4 emissions to the atmosphere. Until recently, aquatic biogenic CH4 production was exclusively attributed to methanogenic archaea living under anoxic or suboxic conditions in sediments, bottom waters, and wetlands. However, evidence for oxic CH4 production (OMP) in freshwater, brackish, and marine habitats is increasing. Possible sources were found to be driven by various planktonic organisms supporting different OMP mechanisms. Surprisingly, submerged macrophytes have been fully ignored in studies on OMP, yet they are key components of littoral zones of ponds, lakes, and coastal systems. High CH4 concentrations in these zones have been attributed to organic substrate production promoting classic methanogenesis in the absence of oxygen. Here, we review existing studies and argue that, similar to terrestrial plants and phytoplankton, macroalgae and submerged macrophytes may directly or indirectly contribute to CH4 formation in oxic waters. We propose several potential direct and indirect mechanisms: (1) direct production of CH4; (2) production of CH4 precursors and facilitation of their bacterial breakdown or chemical conversion; (3) facilitation of classic methanogenesis; and (4) facilitation of CH4 ebullition. As submerged macrophytes occur in many freshwater and marine habitats, they are important in global carbon budgets and can strongly vary in their abundance due to seasonal and boom-bust dynamics. Knowledge on their contribution to OMP is therefore essential to gain a better understanding of spatial and temporal dynamics of CH4 emissions and thus to substantially reduce current uncertainties when estimating global CH4 emissions from aquatic ecosystems.}, language = {en} } @article{TangMcGinnisFrindteetal.2014, author = {Tang, Kam W. and McGinnis, Daniel F. and Frindte, Katharina and Bruchert, Volker and Grossart, Hans-Peter}, title = {Paradox reconsidered: Methane oversaturation in well-oxygenated lake waters}, series = {Limnology and oceanography}, volume = {59}, journal = {Limnology and oceanography}, number = {1}, publisher = {Wiley}, address = {Waco}, issn = {0024-3590}, doi = {10.4319/lo.2014.59.1.0275}, pages = {275 -- 284}, year = {2014}, abstract = {The widely reported paradox of methane oversaturation in oxygenated water challenges the prevailing paradigm that microbial methanogenesis only occurs under anoxic conditions. Using a combination of field sampling, incubation experiments, and modeling, we show that the recurring mid-water methane peak in Lake Stechlin, northeast Germany, was not dependent on methane input from the littoral zone or bottom sediment or on the presence of known micro-anoxic zones. The methane peak repeatedly overlapped with oxygen oversaturation in the seasonal thermocline. Incubation experiments and isotope analysis indicated active methane production, which was likely linked to photosynthesis and/or nitrogen fixation within the oxygenated water, whereas lessening of methane oxidation by light allowed accumulation of methane in the oxygen-rich upper layer. Estimated methane efflux from the surface water was up to 5 mmol m(-2) d(-1). Mid-water methane oversaturation was also observed in nine other lakes that collectively showed a strongly negative gradient of methane concentration within 0-20\% dissolved oxygen (DO) in the bottom water, and a positive gradient within >= 20\% DO in the upper water column. Further investigation into the responsible organisms and biochemical pathways will help improve our understanding of the global methane cycle.}, language = {en} } @article{GuenthelDonisKirillinetal.2019, author = {G{\"u}nthel, Marco and Donis, Daphne and Kirillin, Georgiy and Ionescu, Danny and Bizic, Mina and McGinnis, Daniel F. and Grossart, Hans-Peter and Tang, Kam W.}, title = {Contribution of oxic methane production to surface methane emission in lakes and its global importance}, series = {Nature Communications}, volume = {10}, journal = {Nature Communications}, publisher = {Nature Publishing Group UK}, address = {London}, issn = {2041-1723}, doi = {10.1038/s41467-019-13320-0}, pages = {10}, year = {2019}, abstract = {Recent discovery of oxic methane production in sea and lake waters, as well as wetlands, demands re-thinking of the global methane cycle and re-assessment of the contribution of oxic waters to atmospheric methane emission. Here we analysed system-wide sources and sinks of surface-water methane in a temperate lake. Using a mass balance analysis, we show that internal methane production in well-oxygenated surface water is an important source for surface-water methane during the stratified period. Combining our results and literature reports, oxic methane contribution to emission follows a predictive function of littoral sediment area and surface mixed layer volume. The contribution of oxic methane source(s) is predicted to increase with lake size, accounting for the majority (>50\%) of surface methane emission for lakes with surface areas >1 km(2).}, language = {en} } @article{TangFluryGrossartetal.2017, author = {Tang, Kam W. and Flury, Sabine and Grossart, Hans-Peter and McGinnis, Daniel F.}, title = {The Chaoborus pump: Migrating phantom midge larvae sustain hypolimnetic oxygen deficiency and nutrient internal loading in lakes}, series = {Water research}, volume = {122}, journal = {Water research}, publisher = {Elsevier}, address = {Oxford}, issn = {0043-1354}, doi = {10.1016/j.watres.2017.05.058}, pages = {36 -- 41}, year = {2017}, abstract = {Hypolimnetic oxygen demand in lakes is often assumed to be driven mainly by sediment microbial processes, while the role of Chaoborus larvae, which are prevalent in eutrophic lakes with hypoxic to anoxic bottoms, has been overlooked. We experimentally measured the respiration rates of C flavicans at different temperatures yielding a Q(10) of 1.44-1.71 and a respiratory quotient of 0.84-0.98. Applying the experimental data in a system analytical approach, we showed that migrating Chaoborus larvae can significantly add to the water column and sediment oxygen demand, and contribute to the observed linear relationship between water column respiration and depth. The estimated phosphorus excretion by Chaoborus in sediment is comparable in magnitude to the required phosphorus loading for eutrophication. Migrating Chaoborus larvae thereby essentially trap nutrients between the water column and the sediment, and this continuous internal loading of nutrients would delay lake remediation even when external inputs are stopped. (C) 2017 Elsevier Ltd. All rights reserved.}, language = {en} } @misc{TangMcGinnisIonescuetal.2016, author = {Tang, Kam W. and McGinnis, Daniel F. and Ionescu, Danny and Großart, Hans-Peter}, title = {Methane Production in Oxic Lake Waters Potentially Increases Aquatic Methane Flux to Air}, series = {Physical chemistry, chemical physics : a journal of European Chemical Societies}, volume = {3}, journal = {Physical chemistry, chemical physics : a journal of European Chemical Societies}, publisher = {American Chemical Society}, address = {Washington}, issn = {2328-8930}, doi = {10.1021/acs.estlett.6b00150}, pages = {227 -- 233}, year = {2016}, abstract = {Active methane production in oxygenated lake waters challenges the long-standing paradigm that microbial methane production occurs only under anoxic conditions and forces us to rethink the ecology and environmental dynamics of this powerful greenhouse gas. Methane production in the upper oxic water layers places the methane source closer to the air water interface, where convective mixing and microbubble detrainment can lead to a methane efflux higher than that previously assumed. Microorganisms may produce methane in oxic environments by being equipped with enzymes to counteract the effects of molecular oxygen during methanogenesis or using alternative pathways that do not involve oxygen-sensitive enzymes. As this process appears to be influenced by thermal stratification, water transparency, and primary production, changes in lake ecology due to climate change will alter methane formation in oxic water layers, with far-reaching consequences for methane flux and climate feedback.}, language = {en} } @article{McGinnisFluryTangetal.2017, author = {McGinnis, Daniel F. and Flury, Sabine and Tang, Kam W. and Grossart, Hans-Peter}, title = {Porewater methane transport within the gas vesicles of diurnally migrating Chaoborus spp.}, series = {Scientific reports}, volume = {7}, journal = {Scientific reports}, publisher = {Nature Publ. Group}, address = {London}, issn = {2045-2322}, doi = {10.1038/srep44478}, pages = {7}, year = {2017}, abstract = {Diurnally-migrating Chaoborus spp. reach populations of up to 130,000 individuals m-2 in lakes up to 70 meters deep on all continents except Antarctica. Linked to eutrophication, migrating Chaoborus spp. dwell in the anoxic sediment during daytime and feed in the oxic surface layer at night. Our experiments show that by burrowing into the sediment, Chaoborus spp. utilize the high dissolved gas partial pressure of sediment methane to inflate their tracheal sacs. This mechanism provides a significant energetic advantage that allows the larvae to migrate via passive buoyancy rather than more energy-costly swimming. The Chaoborus spp. larvae, in addition to potentially releasing sediment methane bubbles twice a day by entering and leaving the sediment, also transport porewater methane within their gas vesicles into the water column, resulting in a flux of 0.01-2 mol m-2 yr-1 depending on population density and water depth. Chaoborus spp. emerging annually as flies also result in 0.1-6 mol m-2 yr-1 of carbon export from the system. Finding the tipping point in lake eutrophication enabling this methane-powered migration mechanism is crucial for ultimately reconstructing the geographical expansion of Chaoborus spp., and the corresponding shifts in the lake's biogeochemistry, carbon cycling and food web structure.}, language = {en} }