@article{JueppnerMubeenLeisseetal.2017, author = {J{\"u}ppner, Jessica and Mubeen, Umarah and Leisse, Andrea and Caldana, Camila and Brust, Henrike and Steup, Martin and Herrmann, Marion and Steinhauser, Dirk and Giavalisco, Patrick}, title = {Dynamics of lipids and metabolites during the cell cycle of Chlamydomonas reinhardtii}, series = {The plant journal}, volume = {92}, journal = {The plant journal}, publisher = {Wiley}, address = {Hoboken}, issn = {0960-7412}, doi = {10.1111/tpj.13642}, pages = {331 -- 343}, year = {2017}, abstract = {Metabolites and lipids are the final products of enzymatic processes, distinguishing the different cellular functions and activities of single cells or whole tissues. Understanding these cellular functions within a well-established model system requires a systemic collection of molecular and physiological information. In the current report, the green alga Chlamydomonas reinhardtii was selected to establish a comprehensive workflow for the detailed multi-omics analysis of a synchronously growing cell culture system. After implementation and benchmarking of the synchronous cell culture, a two-phase extraction method was adopted for the analysis of proteins, lipids, metabolites and starch from a single sample aliquot of as little as 10-15million Chlamydomonas cells. In a proof of concept study, primary metabolites and lipids were sampled throughout the diurnal cell cycle. The results of these time-resolved measurements showed that single compounds were not only coordinated with each other in different pathways, but that these complex metabolic signatures have the potential to be used as biomarkers of various cellular processes. Taken together, the developed workflow, including the synchronized growth of the photoautotrophic cell culture, in combination with comprehensive extraction methods and detailed metabolic phenotyping has the potential for use in in-depth analysis of complex cellular processes, providing essential information for the understanding of complex biological systems.}, language = {en} } @misc{TracyHeideWahletal.2009, author = {Tracy, Rosemarie and Heide, Judith and Wahl, Michael and Triarchi-Herrmann, Vassilia and Grimm, Angela and Wotschack, Christiane and Kulik, Sylvia and Frank, Ulrike and Klassert, Annegret and Gagarina, Natalʹja Vladimirovna and Kauschke, Christina and Eicher, Iris and Tsakmaki, Barbara and Akkaya, Zeynep and Castillo, Esmeralda and Groba, Agnes and H{\"o}hle, Barbara and Miertsch, Barbara and Hubert, Anja and Sauerland, Uli and Schr{\"o}der, Caroline and Stadie, Nicole and Wittler, Marion and Berendes, Karin and Gottal, Stephanie and Grabherr, Britta and Zaps, Jennifer and Ptok, Martin and Hanne, Sandra and Sekerina, Irina A. and Vasishth, Shravan and Burchert, Frank and De Bleser, Ria and Kleissendorf, Barbara and Jaecks, Petra and Stenneken, Prisca and Fischer, Ivette and Moedebeck, Petra}, title = {Spektrum Patholinguistik = Schwerpunktthema: Ein Kopf - Zwei Sprachen : Mehrsprachigkeit in Forschung und Therapie}, number = {2}, editor = {Heide, Judith and Hanne, Sandra and Brandt-Kobele, Oda-Christina and Fritzsche, Tom}, publisher = {Universit{\"a}tsverlag Potsdam}, address = {Potsdam}, organization = {Verband f{\"u}r Patholinguistik e. V. (vpl)}, isbn = {978-3-940793-89-8}, issn = {1869-3822}, doi = {10.25932/publishup-3086}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-30451}, year = {2009}, abstract = {"Spektrum Patholinguistik" (Band 2) ist der Tagungsband zum 2. Herbsttreffen Patholinguistik, das der Verband f{\"u}r Patholinguistik (vpl) e.V. am 22.11.2008 an der Universit{\"a}t Potsdam veranstaltet hat. Zum Schwerpunktthema "Ein Kopf - Zwei Sprachen: Mehrsprachigkeit in Forschung und Therapie" sind die drei Hauptvortr{\"a}ge und vier Abstracts von Posterpr{\"a}sentationen ver{\"o}ffentlicht. Desweiteren enth{\"a}lt der Tagungsband freie Beitr{\"a}ge, u.a. zu Satzverarbeitung und Agrammatismus, Lesestrategien und LRS, Prosodie-Entwicklung, kindlichen Aphasien, Dysphagie-Therapie sowie zu kognitiven Defiziten bei {\"a}lteren Menschen.}, language = {de} }