@article{NukarinenNaegelePedrottietal.2016, author = {Nukarinen, Ella and N{\"a}gele, Thomas and Pedrotti, Lorenzo and Wurzinger, Bernhard and Mair, Andrea and Landgraf, Ramona and B{\"o}rnke, Frederik and Hanson, Johannes and Teige, Markus and Baena-Gonzalez, Elena and Dr{\"o}ge-Laser, Wolfgang and Weckwerth, Wolfram}, title = {Quantitative phosphoproteomics reveals the role of the AMPK plant ortholog SnRK1 as a metabolic master regulator under energy deprivation}, series = {Scientific reports}, volume = {6}, journal = {Scientific reports}, publisher = {Nature Publ. Group}, address = {London}, issn = {2045-2322}, doi = {10.1038/srep31697}, pages = {10248 -- 10252}, year = {2016}, abstract = {Since years, research on SnRK1, the major cellular energy sensor in plants, has tried to define its role in energy signalling. However, these attempts were notoriously hampered by the lethality of a complete knockout of SnRK1. Therefore, we generated an inducible amiRNA:: SnRK1 alpha 2 in a snrk1 alpha 1 knock out background (snrk1 alpha 1/alpha 2) to abolish SnRK1 activity to understand major systemic functions of SnRK1 signalling under energy deprivation triggered by extended night treatment. We analysed the in vivo phosphoproteome, proteome and metabolome and found that activation of SnRK1 is essential for repression of high energy demanding cell processes such as protein synthesis. The most abundant effect was the constitutively high phosphorylation of ribosomal protein S6 (RPS6) in the snrk1 alpha 1/alpha 2 mutant. RPS6 is a major target of TOR signalling and its phosphorylation correlates with translation. Further evidence for an antagonistic SnRK1 and TOR crosstalk comparable to the animal system was demonstrated by the in vivo interaction of SnRK1 alpha 1 and RAPTOR1B in the cytosol and by phosphorylation of RAPTOR1B by SnRK1 alpha 1 in kinase assays. Moreover, changed levels of phosphorylation states of several chloroplastic proteins in the snrk1 alpha 1/alpha 2 mutant indicated an unexpected link to regulation of photosynthesis, the main energy source in plants.}, language = {en} } @article{PlatzWeckmannPeketal.2022, author = {Platz, Anna and Weckmann, Ute and Pek, Josef and Kovacikova, Svetlana and Klanica, Radek and Mair, Johannes and Aleid, Basel}, title = {3D imaging of the subsurface electrical resistivity structure in West Bohemia/Upper Palatinate covering mofettes and quaternary volcanic structures by using magnetotellurics}, series = {Tectonophysics : international journal of geotectonics and the geology and physics of the interior of the earth}, volume = {833}, journal = {Tectonophysics : international journal of geotectonics and the geology and physics of the interior of the earth}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0040-1951}, doi = {10.1016/j.tecto.2022.229353}, pages = {20}, year = {2022}, abstract = {The region of West Bohemia and Upper Palatinate belongs to the West Bohemian Massif. The study area is situated at the junction of three different Variscan tectonic units and hosts the ENE-WSW trending Ohre Rift as well as many different fault systems. The entire region is characterized by ongoing magmatic processes in the intra-continental lithospheric mantle expressed by a series of phenomena, including e.g. the occurrence of repeated earthquake swarms and massive degassing of mantle derived CO2 in form of mineral springs and mofettes. Ongoing active tectonics is mainly manifested by Cenozoic volcanism represented by different Quaternary volcanic structures. All these phenomena make the Ohre Rift a unique target area for European intra-continental geo-scientific research. With magnetotelluric (MT) measurements we image the subsurface distribution of the electrical resistivity and map possible fluid pathways. Two-dimensional (2D) inversion results by Munoz et al. (2018) reveal a conductive channel in the vicinity of the earthquake swarm region that extends from the lower crust to the surface forming a pathway for fluids into the region of the mofettes. A second conductive channel is present in the south of their model; however, their 2D inversions allow ambiguous interpretations of this feature. Therefore, we conducted a large 3D MT field experiment extending the study area towards the south. The 3D inversion result matches well with the known geology imaging different fluid/magma reservoirs at crust-mantle depth and mapping possible fluid pathways from the reservoirs to the surface feeding known mofettes and spas. A comparison of 3D and 2D inversion results suggests that the 2D inversion results are considerably characterized by 3D and off-profile structures. In this context, the new results advocate for the swarm earthquakes being located in the resistive host rock surrounding the conductive channels; a finding in line with observations e.g. at the San Andreas Fault, California.}, language = {en} }