@article{SchenkSellrieBoettgeretal.2007, author = {Schenk, J{\"o}rg A. and Sellrie, Frank and B{\"o}ttger, Volker and Micheel, Burkhard and St{\"o}cklein, Walter F. M.}, title = {Generation and application of a fluorescein-specific single chain antibody}, year = {2007}, abstract = {A recombinant single chain antibody fragment (designated scDE1) of the murine monoclonal anti-fluorescein antibody B13-DE1 was generated using the original hybridoma cells as source for the variable antibody heavy and light chain (VH and VL) genes. After cloning the variable genes into a phage vector a functional antibody fragment was selected by phage display panning. Recombinant antibody could be expressed as phage antibody and as soluble single chain antibody in Escherichia coli. High yield of scDE1 could also be detected in bacterial culture supernatant. The scDE1 showed the same binding specificity as the parental monoclonal antibody, i.e. it bound fluorescein, fluorescein derivatives and a fluorescein peptide mimotope. Surface plasmon resonance revealed a K(D) of 19 nM for the scDE1 compared to 0.7 nM for the monoclonal antibody. The isolated soluble scDE1 could easily be conjugated to horseradish peroxidase which allowed the use of the conjugate as universal indicator for the detection of fluorescein-labelled proteins in different immunoassays. Detection of hCG in urine was performed as a model system using scDE1. In addition to E. coli the scFv genes could also be transferred and expressed in eukaryotic cells. Finally, we generated HEK293 cells expressing the scDE1 at the cell surface.}, language = {en} } @article{LawatscheckAleksaiteSchenketal.2007, author = {Lawatscheck, Robert and Aleksaite, Egle and Schenk, J{\"o}rg A. and Micheel, Burkhard and Jandrig, Burkhard and Holland, Gudrun and Sasnauskas, Kestutius and Gedvilaite, Alma and Ulrich, Rainer G{\"u}nter}, title = {Chimeric polyomavirus-derived virus-like particles : the immunogenicity of an inserted peptide applied without adjuvant to mice depends on its insertion site and its flanking linker sequence}, issn = {0882-8245}, doi = {10.1089/vim.2007.0023}, year = {2007}, abstract = {We inserted the sequence of the carcinoembryonic antigen-derived T cell epitope CAP-1-6D (CEA) into different positions of the hamster polyomavirus major capsid protein VP1. Independently from additional flanking linkers, yeast- expressed VP1 proteins harboring the CEA insertion between VP1 amino acid residues 80 and 89 (site 1) or 288 and 295 (site 4) or simultaneously at both positions assembled to chimeric virus-like particles (VLPs). BALB/c mice immunized with adjuvant-free VLPs developed VP1- and epitope-specific antibodies. The level of the CEA-specific antibody response was determined by the insertion site, the number of inserts, and the flanking linker. The strongest CEA-specific antibody response was observed in mice immunized with VP1 proteins harboring the CEA insert at site 1. Moreover, the CEA- specific antibodies in these mice were still detectable 6 mo after the final booster immunization. Our results indicate that hamster polyomavirus-derived VLPs represent a highly immunogenic carrier for foreign insertions that might be useful for clinical and therapeutic applications.}, language = {en} } @article{PagelFritzschBiedermannetal.2008, author = {Pagel, J{\"o}rn and Fritzsch, Katrin and Biedermann, Robert and Schr{\"o}der-Esselbach, Boris}, title = {Annual plants under cyclic disturbance regime : better understanding through model aggregation}, issn = {1051-0761}, year = {2008}, abstract = {In their application for conservation ecology, 'classical' analytical models and individual-based simulation models (IBMs) both entail their specific strengths and weaknesses, either in providing a detailed and realistic representation of processes or in regard to a comprehensive model analysis. This well-known dilemma may be resolved by the combination of both approaches when tackling certain problems of conservation ecology. Following this idea, we present the complementary use of both an IBM and a matrix population model in a case study on grassland conservation management. First, we develop a spatially explicit IBM to simulate the long-term response of the annual plant Thlaspi perfoliatum (Brassicaceae), claspleaf pennycress, to different management schemes (annual mowing vs. infrequent rototilling) based on field experiments. In order to complement the simulation results by further analyses, we aggregate the IBM to a spatially nonexplicit deterministic matrix population model. Within the periodic environment created by management regimes, population dynamics are described by periodic products of annual transition matrices. Such periodic matrix products provide a very conclusive framework to study the responses of species to different management return intervals. Thus, using tools of matrix model analysis (e.g., loop analysis), we can both identify dormancy within the age-structured seed bank as the pivotal strategy for persistence under cyclic disturbance regimes and reveal crucial thresholds in some less certain parameters. Results of matrix model analyses are therefore successfully tested by comparing their results to the respective IBM simulations. Their implications for an enhanced scientific basis for management decisions are discussed as well as some general benefits and limitations of the use of aggregating modeling approaches in conservation.}, language = {en} } @article{FranzkeBoogersRuanoetal.2007, author = {Franzke, Jochen and Boogers, Marcel and Ruano, Jose M. and Schaap, Linze}, title = {Conclusions : Governance and Democracy at the Local Level - Structures, Processes and Tensions}, isbn = {978-90-352-4176-3}, year = {2007}, language = {en} } @article{FranzkeBoogersRuanoetal.2007, author = {Franzke, Jochen and Boogers, Marcel and Ruano, Jose M. and Schaap, Linze}, title = {Introduction}, isbn = {978-90-352-4176-3}, year = {2007}, language = {en} } @book{FranzkeBoogersRuanoetal.2007, author = {Franzke, Jochen and Boogers, Marcel and Ruano, Jose M. and Schaap, Linze}, title = {Tensions between local governance and local democracy}, series = {Local Governance and Democracy}, volume = {1}, journal = {Local Governance and Democracy}, publisher = {Reed Business}, address = {The Hague}, isbn = {978-90-352-4176-3}, pages = {303 S.}, year = {2007}, language = {en} } @phdthesis{Bulut2010, author = {Bulut, Fatih}, title = {Seismicity and fault kinematics of the North Anatolian fault zone along the 1999 Izmit rupture and its transition to the Marmara seismic gap}, pages = {VII, 98 S.}, year = {2010}, language = {en} } @article{WilkePartzschWelteretal.2007, author = {Wilke, Max and Partzsch, G. M. and Welter, E. and Farges, Francois}, title = {Redox Reaction In Silicate Melts Monitored By Static In-Situ Fe K-edge XANES Up To 1180 C}, year = {2007}, language = {en} } @article{WilkeFargesPartzschetal.2007, author = {Wilke, Max and Farges, Francois and Partzsch, G. M. and Schmidt, C. and Behrens, Harald}, title = {Speciation of Fe in silicate glasses and melts by in-situ XANES spectroscopy}, year = {2007}, language = {en} } @phdthesis{Koseska2007, author = {Koseska, Aneta}, title = {Modeling and control of synthetic gene regulatory networks}, address = {Potsdam}, pages = {viii, 82 S.}, year = {2007}, language = {en} }