@article{RadosavljevicLantuitKnoblauchetal.2022, author = {Radosavljevic, Boris and Lantuit, Hugues and Knoblauch, Christian and Couture, Nicole and Herzschuh, Ulrike and Fritz, Michael}, title = {Arctic nearshore sediment dynamics - an example from Herschel Island - Qikiqtaruk, Canada}, series = {Journal of marine science and engineering}, volume = {10}, journal = {Journal of marine science and engineering}, number = {11}, publisher = {MDPI}, address = {Basel}, issn = {2077-1312}, doi = {10.3390/jmse10111589}, pages = {18}, year = {2022}, abstract = {Increasing arctic coastal erosion rates imply a greater release of sediments and organic matter into the coastal zone. With 213 sediment samples taken around Herschel Island-Qikiqtaruk, Canadian Beaufort Sea, we aimed to gain new insights on sediment dynamics and geochemical properties of a shallow arctic nearshore zone. Spatial characteristics of nearshore sediment texture (moderately to poorly sorted silt) are dictated by hydrodynamic processes, but ice-related processes also play a role. We determined organic matter (OM) distribution and inferred the origin and quality of organic carbon by C/N ratios and stable carbon isotopes delta C-13. The carbon content was higher offshore and in sheltered areas (mean: 1.0 wt.\%., S.D.: 0.9) and the C/N ratios also showed a similar spatial pattern (mean: 11.1, S.D.: 3.1), while the delta C-13 (mean: -26.4 parts per thousand VPDB, S.D.: 0.4) distribution was more complex. We compared the geochemical parameters of our study with terrestrial and marine samples from other studies using a bootstrap approach. Sediments of the current study contained 6.5 times and 1.8 times less total organic carbon than undisturbed and disturbed terrestrial sediments, respectively. Therefore, degradation of OM and separation of carbon pools take place on land and continue in the nearshore zone, where OM is leached, mineralized, or transported beyond the study area.}, language = {en} } @article{BalintMartonSchatzetal.2018, author = {Balint, Miklos and Marton, Orsolya and Schatz, Marlene and D{\"u}ring, Rolf-Alexander and Grossart, Hans-Peter}, title = {Proper experimental design requires randomization/balancing of molecular ecology experiments}, series = {Ecology and evolution}, volume = {8}, journal = {Ecology and evolution}, number = {3}, publisher = {Wiley}, address = {Hoboken}, issn = {2045-7758}, doi = {10.1002/ece3.3687}, pages = {1786 -- 1793}, year = {2018}, abstract = {Properly designed (randomized and/or balanced) experiments are standard in ecological research. Molecular methods are increasingly used in ecology, but studies generally do not report the detailed design of sample processing in the laboratory. This may strongly influence the interpretability of results if the laboratory procedures do not account for the confounding effects of unexpected laboratory events. We demonstrate this with a simple experiment where unexpected differences in laboratory processing of samples would have biased results if randomization in DNA extraction and PCR steps do not provide safeguards. We emphasize the need for proper experimental design and reporting of the laboratory phase of molecular ecology research to ensure the reliability and interpretability of results.}, language = {en} } @article{FranckeBaroniBrosinskyetal.2018, author = {Francke, Till and Baroni, Gabriele and Brosinsky, Arlena and Foerster, Saskia and Lopez-Tarazon, Jos{\´e} Andr{\´e}s and Sommerer, Erik and Bronstert, Axel}, title = {What Did Really Improve Our Mesoscale Hydrological Model?}, series = {Water resources research}, volume = {54}, journal = {Water resources research}, number = {11}, publisher = {American Geophysical Union}, address = {Washington}, issn = {0043-1397}, doi = {10.1029/2018WR022813}, pages = {8594 -- 8612}, year = {2018}, abstract = {Modelers can improve a model by addressing the causes for the model errors (data errors and structural errors). This leads to implementing model enhancements (MEs), for example, meteorological data based on more monitoring stations, improved calibration data, and/or modifications in process formulations. However, deciding on which MEs to implement remains a matter of expert knowledge. After implementing multiple MEs, any improvement in model performance is not easily attributed, especially when considering different objectives or aspects of this improvement (e.g., better dynamics vs. reduced bias). We present an approach for comparing the effect of multiple MEs based on real observations and considering multiple objectives (MMEMO). A stepwise selection approach and structured plots help to address the multidimensionality of the problem. Tailored analyses allow a differentiated view on the effect of MEs and their interactions. MMEMO is applied to a case study employing the mesoscale hydro-sedimentological model WASA-SED for the Mediterranean-mountainous Isabena catchment, northeast Spain. The investigated seven MEs show diverse effects: some MEs (e.g., rainfall data) cause improvements for most objectives, while other MEs (e.g., land use data) only affect a few objectives or even decrease model performance. Interaction of MEs was observed for roughly half of the MEs, confirming the need to address them in the analysis. Calibration and increasing the temporal resolution showed by far stronger impact than any of the other MEs. The proposed framework can be adopted in other studies to analyze the effect of MEs and, thus, facilitate the identification and implementation of the most promising MEs for comparable cases.}, language = {en} } @article{KocSimovichSchoenemannetal.2019, author = {Koc, Julian and Simovich, Tomer and Sch{\"o}nemann, Eric and Chilkoti, Ashutosh and Gardner, Harrison and Swain, Geoffrey W. and Hunsucker, Kelli and Laschewsky, Andr{\´e} and Rosenhahn, Axel}, title = {Sediment challenge to promising ultra-low fouling hydrophilic surfaces in the marine environment}, series = {Biofouling : the journal of bioadhesion and biofilm research}, volume = {35}, journal = {Biofouling : the journal of bioadhesion and biofilm research}, number = {4}, publisher = {Taylor \& Francis}, address = {London}, issn = {0892-7014}, doi = {10.1080/08927014.2019.1611790}, pages = {454 -- 462}, year = {2019}, abstract = {Hydrophilic coatings exhibit ultra-low fouling properties in numerous laboratory experiments. In stark contrast, the antifouling effect of such coatings in vitro failed when performing field tests in the marine environment. The fouling release performance of nonionic and zwitterionic hydrophilic polymers was substantially reduced compared to the controlled laboratory environment. Microscopy and spectroscopy revealed that a large proportion of the accumulated material in field tests contains inorganic compounds and diatomaceous soil. Diatoms adhered to the accumulated material on the coating, but not to the pristine polymer. Simulating field tests in the laboratory using sediment samples collected from the test sites showed that incorporated sand and diatomaceous earth impairs the fouling release characteristics of the coatings. When exposed to marine sediment from multiple locations, particulate matter accumulated on these coatings and served as attachment points for diatom adhesion and enhanced fouling. Future developments of hydrophilic coatings should consider accumulated sediment and its potential impact on the antifouling performance.}, language = {en} } @misc{WurzbacherFuchsAttermeyeretal.2017, author = {Wurzbacher, Christian and Fuchs, Andrea and Attermeyer, Katrin and Frindte, Katharina and Grossart, Hans-Peter and Hupfer, Michael and Casper, Peter and Monaghan, Michael T.}, title = {Shifts among Eukaryota, Bacteria, and Archaea define the vertical organization of a lake sediment}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {1111}, issn = {1866-8372}, doi = {10.25932/publishup-43196}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-431965}, pages = {18}, year = {2017}, abstract = {Background Lake sediments harbor diverse microbial communities that cycle carbon and nutrients while being constantly colonized and potentially buried by organic matter sinking from the water column. The interaction of activity and burial remained largely unexplored in aquatic sediments. We aimed to relate taxonomic composition to sediment biogeochemical parameters, test whether community turnover with depth resulted from taxonomic replacement or from richness effects, and to provide a basic model for the vertical community structure in sediments. Methods We analyzed four replicate sediment cores taken from 30-m depth in oligo-mesotrophic Lake Stechlin in northern Germany. Each 30-cm core spanned ca. 170 years of sediment accumulation according to 137Cs dating and was sectioned into layers 1-4 cm thick. We examined a full suite of biogeochemical parameters and used DNA metabarcoding to examine community composition of microbial Archaea, Bacteria, and Eukaryota. Results Community β-diversity indicated nearly complete turnover within the uppermost 30 cm. We observed a pronounced shift from Eukaryota- and Bacteria-dominated upper layers (<5 cm) to Bacteria-dominated intermediate layers (5-14 cm) and to deep layers (>14 cm) dominated by enigmatic Archaea that typically occur in deep-sea sediments. Taxonomic replacement was the prevalent mechanism in structuring the community composition and was linked to parameters indicative of microbial activity (e.g., CO2 and CH4 concentration, bacterial protein production). Richness loss played a lesser role but was linked to conservative parameters (e.g., C, N, P) indicative of past conditions. Conclusions By including all three domains, we were able to directly link the exponential decay of eukaryotes with the active sediment microbial community. The dominance of Archaea in deeper layers confirms earlier findings from marine systems and establishes freshwater sediments as a potential low-energy environment, similar to deep sea sediments. We propose a general model of sediment structure and function based on microbial characteristics and burial processes. An upper "replacement horizon" is dominated by rapid taxonomic turnover with depth, high microbial activity, and biotic interactions. A lower "depauperate horizon" is characterized by low taxonomic richness, more stable "low-energy" conditions, and a dominance of enigmatic Archaea.}, language = {en} } @article{DommainAndamaMcDonoughetal.2020, author = {Dommain, Ren{\´e} and Andama, Morgan and McDonough, Molly M. and Prado, Natalia A. and Goldhammer, Tobias and Potts, Richard and Maldonado, Jes{\´u}s E. and Nkurunungi, John Bosco and Campana, Michael G.}, title = {The Challenges of Reconstructing Tropical Biodiversity With Sedimentary Ancient DNA}, series = {Frontiers in Ecology and Evolution}, volume = {8}, journal = {Frontiers in Ecology and Evolution}, publisher = {Frontiers Media}, address = {Lausanne}, issn = {2296-701X}, doi = {10.3389/fevo.2020.00218}, pages = {26}, year = {2020}, abstract = {Sedimentary ancient DNA has been proposed as a key methodology for reconstructing biodiversity over time. Yet, despite the concentration of Earth's biodiversity in the tropics, this method has rarely been applied in this region. Moreover, the taphonomy of sedimentary DNA, especially in tropical environments, is poorly understood. This study elucidates challenges and opportunities of sedimentary ancient DNA approaches for reconstructing tropical biodiversity. We present shotgun-sequenced metagenomic profiles and DNA degradation patterns from multiple sediment cores from Mubwindi Swamp, located in Bwindi Impenetrable Forest (Uganda), one of the most diverse forests in Africa. We describe the taxonomic composition of the sediments covering the past 2200 years and compare the sedimentary DNA data with a comprehensive set of environmental and sedimentological parameters to unravel the conditions of DNA degradation. Consistent with the preservation of authentic ancient DNA in tropical swamp sediments, DNA concentration and mean fragment length declined exponentially with age and depth, while terminal deamination increased with age. DNA preservation patterns cannot be explained by any environmental parameter alone, but age seems to be the primary driver of DNA degradation in the swamp. Besides degradation, the presence of living microbial communities in the sediment also affects DNA quantity. Critically, 92.3\% of our metagenomic data of a total 81.8 million unique, merged reads cannot be taxonomically identified due to the absence of genomic references in public databases. Of the remaining 7.7\%, most of the data (93.0\%) derive from Bacteria and Archaea, whereas only 0-5.8\% are from Metazoa and 0-6.9\% from Viridiplantae, in part due to unbalanced taxa representation in the reference data. The plant DNA record at ordinal level agrees well with local pollen data but resolves less diversity. Our animal DNA record reveals the presence of 41 native taxa (16 orders) including Afrotheria, Carnivora, and Ruminantia at Bwindi during the past 2200 years. Overall, we observe no decline in taxonomic richness with increasing age suggesting that several-thousand-year-old information on past biodiversity can be retrieved from tropical sediments. However, comprehensive genomic surveys of tropical biota need prioritization for sedimentary DNA to be a viable methodology for future tropical biodiversity studies.}, language = {en} } @misc{DommainAndamaMcDonoughetal.2020, author = {Dommain, Ren{\´e} and Andama, Morgan and McDonough, Molly M. and Prado, Natalia A. and Goldhammer, Tobias and Potts, Richard and Maldonado, Jes{\´u}s E. and Nkurunungi, John Bosco and Campana, Michael G.}, title = {The Challenges of Reconstructing Tropical Biodiversity With Sedimentary Ancient DNA}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {970}, issn = {1866-8372}, doi = {10.25932/publishup-47430}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-474305}, pages = {28}, year = {2020}, abstract = {Sedimentary ancient DNA has been proposed as a key methodology for reconstructing biodiversity over time. Yet, despite the concentration of Earth's biodiversity in the tropics, this method has rarely been applied in this region. Moreover, the taphonomy of sedimentary DNA, especially in tropical environments, is poorly understood. This study elucidates challenges and opportunities of sedimentary ancient DNA approaches for reconstructing tropical biodiversity. We present shotgun-sequenced metagenomic profiles and DNA degradation patterns from multiple sediment cores from Mubwindi Swamp, located in Bwindi Impenetrable Forest (Uganda), one of the most diverse forests in Africa. We describe the taxonomic composition of the sediments covering the past 2200 years and compare the sedimentary DNA data with a comprehensive set of environmental and sedimentological parameters to unravel the conditions of DNA degradation. Consistent with the preservation of authentic ancient DNA in tropical swamp sediments, DNA concentration and mean fragment length declined exponentially with age and depth, while terminal deamination increased with age. DNA preservation patterns cannot be explained by any environmental parameter alone, but age seems to be the primary driver of DNA degradation in the swamp. Besides degradation, the presence of living microbial communities in the sediment also affects DNA quantity. Critically, 92.3\% of our metagenomic data of a total 81.8 million unique, merged reads cannot be taxonomically identified due to the absence of genomic references in public databases. Of the remaining 7.7\%, most of the data (93.0\%) derive from Bacteria and Archaea, whereas only 0-5.8\% are from Metazoa and 0-6.9\% from Viridiplantae, in part due to unbalanced taxa representation in the reference data. The plant DNA record at ordinal level agrees well with local pollen data but resolves less diversity. Our animal DNA record reveals the presence of 41 native taxa (16 orders) including Afrotheria, Carnivora, and Ruminantia at Bwindi during the past 2200 years. Overall, we observe no decline in taxonomic richness with increasing age suggesting that several-thousand-year-old information on past biodiversity can be retrieved from tropical sediments. However, comprehensive genomic surveys of tropical biota need prioritization for sedimentary DNA to be a viable methodology for future tropical biodiversity studies.}, language = {en} } @article{WurzbacherWarthmannBourneetal.2016, author = {Wurzbacher, Christian and Warthmann, Norman and Bourne, Elizabeth Charlotte and Attermeyer, Katrin and Allgaier, Martin and Powell, Jeff R. and Detering, Harald and Mbedi, Susan and Großart, Hans-Peter and Monaghan, Michael T.}, title = {High habitat-specificity in fungal communities in oligo-mesotrophic, temperate Lake Stechlin (North-East Germany)}, series = {MycoKeys}, volume = {41}, journal = {MycoKeys}, publisher = {Pensoft Publ.}, address = {Sofia}, issn = {1314-4057}, doi = {10.3897/mycokeys.16.9646}, pages = {17 -- 44}, year = {2016}, abstract = {Freshwater fungi are a poorly studied ecological group that includes a high taxonomic diversity. Most studies on aquatic fungal diversity have focused on single habitats, thus the linkage between habitat heterogeneity and fungal diversity remains largely unexplored. We took 216 samples from 54 locations representing eight different habitats in the meso-oligotrophic, temperate Lake Stechlin in North-East Germany. These included the pelagic and littoral water column, sediments, and biotic substrates. We performed high throughput sequencing using the Roche 454 platform, employing a universal eukaryotic marker region within the large ribosomal subunit (LSU) to compare fungal diversity, community structure, and species turnover among habitats. Our analysis recovered 1027 fungal OTUs (97\% sequence similarity). Richness estimates were highest in the sediment, biofilms, and benthic samples (189-231 OTUs), intermediate in water samples (42-85 OTUs), and lowest in plankton samples (8 OTUs). NMDS grouped the eight studied habitats into six clusters, indicating that community composition was strongly influenced by turnover among habitats. Fungal communities exhibited changes at the phylum and order levels along three different substrate categories from littoral to pelagic habitats. The large majority of OTUs (> 75\%) could not be classified below the order level due to the lack of aquatic fungal entries in public sequence databases. Our study provides a first estimate of lake-wide fungal diversity and highlights the important contribution of habitat heterogeneity to overall diversity and community composition. Habitat diversity should be considered in any sampling strategy aiming to assess the fungal diversity of a water body.}, language = {en} } @misc{BalintMartonSchatzetal.2018, author = {B{\´a}lint, Mikl{\´o}s and M{\´a}rton, Orsolya and Schatz, Marlene and D{\"u}ring, Rolf-Alexander and Grossart, Hans-Peter}, title = {Proper experimental design requires randomization/balancing of molecular ecology experiments}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {616}, issn = {1866-8372}, doi = {10.25932/publishup-42387}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-423878}, pages = {8}, year = {2018}, abstract = {Properly designed (randomized and/or balanced) experiments are standard in ecological research. Molecular methods are increasingly used in ecology, but studies generally do not report the detailed design of sample processing in the laboratory. This may strongly influence the interpretability of results if the laboratory procedures do not account for the confounding effects of unexpected laboratory events. We demonstrate this with a simple experiment where unexpected differences in laboratory processing of samples would have biased results if randomization in DNA extraction and PCR steps do not provide safeguards. We emphasize the need for proper experimental design and reporting of the laboratory phase of molecular ecology research to ensure the reliability and interpretability of results.}, language = {en} } @misc{EgholmAndersenFaurschouKnudsenetal.2015, author = {Egholm, David L. and Andersen, Jane Lund and Faurschou Knudsen, Mads and Jansen, John D. and Nielsen, S. B.}, title = {The periglacial engine of mountain erosion}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {552}, issn = {1866-8372}, doi = {10.25932/publishup-40971}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-409718}, pages = {20}, year = {2015}, abstract = {There is growing recognition of strong periglacial control on bedrock erosion in mountain landscapes, including the shaping of low-relief surfaces at high elevations (summit flats). But, as yet, the hypothesis that frost action was crucial to the assumed Late Cenozoic rise in erosion rates remains compelling and untested. Here we present a landscape evolution model incorporating two key periglacial processes - regolith production via frost cracking and sediment transport via frost creep - which together are harnessed to variations in temperature and the evolving thickness of sediment cover. Our computational experiments time-integrate the contribution of frost action to shaping mountain topography over million-year timescales, with the primary and highly reproducible outcome being the development of flattish or gently convex summit flats. A simple scaling of temperature to marine delta O-18 records spanning the past 14 Myr indicates that the highest summit flats in mid-to high-latitude mountains may have formed via frost action prior to the Quaternary. We suggest that deep cooling in the Quaternary accelerated mechanical weathering globally by significantly expanding the area subject to frost. Further, the inclusion of subglacial erosion alongside periglacial processes in our computational experiments points to alpine glaciers increasing the long-term efficiency of frost-driven erosion by steepening hillslopes.}, language = {en} } @misc{MarcHovius2015, author = {Marc, Odin and Hovius, Niels}, title = {Amalgamation in landslide maps}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {485}, issn = {1866-8372}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-408075}, pages = {11}, year = {2015}, abstract = {Inventories of individually delineated landslides are a key to understanding landslide physics and mitigating their impact. They permit assessment of area-frequency distributions and landslide volumes, and testing of statistical correlations between landslides and physical parameters such as topographic gradient or seismic strong motion. Amalgamation, i.e. the mapping of several adjacent landslides as a single polygon, can lead to potentially severe distortion of the statistics of these inventories. This problem can be especially severe in data sets produced by automated mapping. We present five inventories of earthquake-induced landslides mapped with different materials and techniques and affected by varying degrees of amalgamation. Errors on the total landslide volume and power-law exponent of the area-frequency distribution, resulting from amalgamation, may be up to 200 and 50\%, respectively. We present an algorithm based on image and digital elevation model (DEM) analysis, for automatic identification of amalgamated polygons. On a set of about 2000 polygons larger than 1000 m2, tracing landslides triggered by the 1994 Northridge earthquake, the algorithm performs well, with only 2.7-3.6\% incorrectly amalgamated landslides missed and 3.9-4.8\% correct polygons incorrectly identified as amalgams. This algorithm can be used broadly to check landslide inventories and allow faster correction by automating the identification of amalgamation.}, language = {en} } @phdthesis{Francke2009, author = {Francke, Till}, title = {Measurement and modelling of water and sediment fluxes in meso-scale dryland catchments}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-31525}, school = {Universit{\"a}t Potsdam}, year = {2009}, abstract = {Water shortage is a serious threat for many societies worldwide. In drylands, water management measures like the construction of reservoirs are affected by eroded sediments transported in the rivers. Thus, the capability of assessing water and sediment fluxes at the river basin scale is of vital importance to support management decisions and policy making. This subject was addressed by the DFG-funded SESAM-project (Sediment Export from large Semi-Arid catchments: Measurements and Modelling). As a part of this project, this thesis focuses on (1) the development and implementation of an erosion module for a meso-scale catchment model, (2) the development of upscaling and generalization methods for the parameterization of such model, (3) the execution of measurements to obtain data required for the modelling and (4) the application of the model to different study areas and its evaluation. The research was carried out in two meso-scale dryland catchments in NE-Spain: Ribera Salada (200 km²) and Is{\´a}bena (450 km²). Adressing objective 1, WASA-SED, a spatially semi-distributed model for water and sediment transport at the meso-scale was developed. The model simulates runoff and erosion processes at the hillslope scale, transport processes of suspended and bedload fluxes in the river reaches, and retention and remobilisation processes of sediments in reservoirs. This thesis introduces the model concept, presents current model applications and discusses its capabilities and limitations. Modelling at larger scales faces the dilemma of describing relevant processes while maintaining a manageable demand for input data and computation time. WASA-SED addresses this challenge by employing an innovative catena-based upscaling approach: the landscape is represented by characteristic toposequences. For deriving these toposequences with regard to multiple attributes (eg. topography, soils, vegetation) the LUMP-algorithm (Landscape Unit Mapping Program) was developed and related to objective 2. It incorporates an algorithm to retrieve representative catenas and their attributes, based on a Digital Elevation Model and supplemental spatial data. These catenas are classified to provide the discretization for the WASA-SED model. For objective 3, water and sediment fluxes were monitored at the catchment outlet of the Is{\´a}bena and some of its sub-catchments. For sediment yield estimation, the intermittent measurements of suspended sediment concentration (SSC) had to be interpolated. This thesis presents a comparison of traditional sediment rating curves (SRCs), generalized linear models (GLMs) and non-parametric regression using Random Forests (RF) and Quantile Regression Forests (QRF). The observed SSCs are highly variable and range over six orders of magnitude. For these data, traditional SRCs performed poorly, as did GLMs, despite including other relevant process variables (e.g. rainfall intensities, discharge characteristics). RF and QRF proved to be very robust and performed favourably for reproducing sediment dynamics. QRF additionally excels in providing estimates on the accuracy of the predictions. Subsequent analysis showed that most of the sediment was exported during intense storms of late summer. Later floods yielded successively less sediment. Comparing sediment generation to yield at the outlet suggested considerable storage effects within the river channel. Addressing objective 4, the WASA-SED model was parameterized for the two study areas in NE Spain and applied with different foci. For Ribera Salada, the uncalibrated model yielded reasonable results for runoff and sediment. It provided quantitative measures of the change in runoff and sediment yield for different land-uses. Additional land management scenarios were presented and compared to impacts caused by climate change projections. In contrast, the application for the Is{\´a}bena focussed on exploring the full potential of the model's predictive capabilities. The calibrated model achieved an acceptable performance for the validation period in terms of water and sediment fluxes. The inadequate representation of the lower sub-catchments inflicted considerable reductions on model performance, while results for the headwater catchments showed good agreement despite stark contrasts in sediment yield. In summary, the application of WASA-SED to three catchments proved the model framework to be a practicable multi-scale approach. It successfully links the hillslope to the catchment scale and integrates the three components hillslope, river and reservoir in one model. Thus, it provides a feasible approach for tackling issues of water and sediment yield at the meso-scale. The crucial role of processes like transmission losses and sediment storage in the river has been identified. Further advances can be expected when the representation of connectivity of water and sediment fluxes (intra-hillslope, hillslope-river, intra-river) is refined and input data improves.}, language = {en} }