@article{RieckGeigerMunkertetal.2019,
  author    = {Rieck, Christoph Paul Kurt and Geiger, Daniel and Munkert, Jennifer and Messerschmidt, Katrin and Petersen, Jan and Strasser, Juliane and Meitinger, Nadine and Kreis, Wolfgang},
  title     = {Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae},
  series = {Microbiologyopen},
  volume    = {8},
  journal   = {Microbiologyopen},
  number    = {12},
  publisher = {Wiley},
  address   = {Hoboken},
  issn      = {2045-8827},
  doi       = {10.1002/mbo3.925},
  pages     = {11},
  year      = {2019},
  abstract  = {A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ5-3β-hydroxysteroid dehydrogenase gene from Digitalis lanata, (b) a steroid Δ5-isomerase gene from Comamonas testosteronii, (c) a mutated steroid-5β-reductase gene from Arabidopsis thaliana, and (d) a steroid 21-hydroxylase gene from Mus musculus. A second plasmid bearing an ADR/ADX fusion gene from Bos taurus was also constructed. A Saccharomyces cerevisiae strain bearing these two plasmids was generated. This strain, termed "CARD II yeast", was capable of producing 5β-pregnane-3β,21-diol-20-one, a central intermediate in 5β-cardenolide biosynthesis, starting from pregnenolone which was added to the culture medium. Using this approach, five consecutive steps in cardenolide biosynthesis were realized in baker's yeast.},
  language  = {en}
}