@article{WellertTierschKoetzetal.2011,
  author    = {Wellert, Stefan and Tiersch, Brigitte and Koetz, Joachim and Richardt, Andre and Lapp, Alain and Holderer, Olaf and Gaeb, Juergen and Blum, Marc-Michael and Schulreich, Christoph and Stehle, Ralf and Hellweg, Thomas},
  title     = {The DFPase from Loligo vulgaris in sugar surfactant-based bicontinuous microemulsions structure, dynamics, and enzyme activity},
  series = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  volume    = {40},
  journal   = {European biophysics journal : with biophysics letters ; an international journal of biophysics},
  number    = {6},
  publisher = {Springer},
  address   = {New York},
  issn      = {0175-7571},
  doi       = {10.1007/s00249-011-0689-0},
  pages     = {761 -- 774},
  year      = {2011},
  abstract  = {The enzyme diisopropyl fluorophosphatase (DFPase) from the squid Loligo vulgaris is of great interest because of its ability to catalyze the hydrolysis of highly toxic organophosphates. In this work, the enzyme structure in solution (native state) was studied by use of different scattering methods. The results are compared with those from hydrodynamic model calculations based on the DFPase crystal structure. Bicontinuous microemulsions made of sugar surfactants are discussed as host systems for the DFPase. The microemulsion remains stable in the presence of the enzyme, which is shown by means of scattering experiments. Moreover, activity assays reveal that the DFPase still has high activity in this complex reaction medium. To complement the scattering experiments cryo-SEM was also employed to study the microemulsion structure.},
  language  = {en}
}
@article{LemkePrietzelKoetz2013,
  author    = {Lemke, Karina and Prietzel, Claudia Christina and Koetz, Joachim},
  title     = {Fluorescent gold clusters synthesized in a poly(ethyleneimine) modified reverse microemulsion},
  series = {Journal of colloid and interface science},
  volume    = {394},
  journal   = {Journal of colloid and interface science},
  publisher = {Elsevier},
  address   = {San Diego},
  issn      = {0021-9797},
  doi       = {10.1016/j.jcis.2012.11.057},
  pages     = {141 -- 146},
  year      = {2013},
  abstract  = {This paper is focused on the formation of gold clusters in a tailor-made polyelectrolyte-modified reverse microemulsion using poly(ethyleneimine) (PEI) as a cationic polyelectrolyte. PEI incorporated into a ternary w/o microemulsion consisting of water/heptanol/zwitterionic surfactant 3-(N,N-dimethyl-dodecylammonio)-propanesulfonate (SB) acts as a reducing and stabilizing agent and shows an additional template effect. The nanoparticle synthesis is performed by a simple mixing of two microemulsions, one containing the PEI and the other one containing the gold chloride precursor. UV-vis measurements in the microemulsion show two pronounced absorption maxima, one at 360 nm and the other one at 520 nm, indicating two particle fractions. The absorption maximum at 360 nm in combination to the unique fluorescence properties indicate the formation of gold clusters. After a complete solvent evaporation the redispersed nanoparticles have been characterized by using UV-vis and fluorescence spectroscopy, in combination to dynamic light scattering and transmission electron microscopy (TEM). In addition to the gold nanoparticle fraction (>5 nm) the fluorescent gold cluster fraction (<2 nm) can be redispersed without particle aggregation. By means of asymmetric flow field flow fractionation (AF-FFF) two different cluster fractions with particle diameter (<2 nm) can be identified.},
  language  = {en}
}