@article{LauxBierHoelzel2018, author = {Laux, Eva-Maria and Bier, Frank Fabian and H{\"o}lzel, Ralph}, title = {Dielectrophoretic Stretching of DNA}, series = {DNA Nanotechnology}, journal = {DNA Nanotechnology}, edition = {2}, publisher = {Humana Press Inc.}, address = {New York}, isbn = {978-1-4939-8582-1}, issn = {1064-3745}, doi = {10.1007/978-1-4939-8582-1_14}, pages = {199 -- 208}, year = {2018}, abstract = {The spatial control of DNA and of self-assembled DNA constructs is a prerequisite for the preparation of DNA-based nanostructures and microstructures and a useful tool for studies on single DNA molecules. Here we describe a protocol for the accumulation of dissolved lambda-DNA molecules between planar microelectrodes by the action of inhomogeneous radiofrequency electric fields. The resulting AC electrokinetic forces stretch the DNA molecules and align them parallel to the electric field. The electrode preparation from off-the-shelf electronic components is explained, and a detailed description of the electronic setup is given. The experimental procedure is controlled in real-time by fluorescence microscopy.}, language = {en} } @article{Sugita2012, author = {Sugita, Yuko}, title = {Minimal affect uptake in a pre-climax position of conversational "scary" stories}, series = {Journal of pragmatics : an interdisciplinary journal of language studies}, volume = {44}, journal = {Journal of pragmatics : an interdisciplinary journal of language studies}, number = {10}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0378-2166}, doi = {10.1016/j.pragma.2012.05.012}, pages = {1273 -- 1289}, year = {2012}, abstract = {Analyzing pre-climax positions of everyday affect-laden telling activities, this paper shows that particular facial expressions, such as raised eyebrows with an open mouth or jaw-dropping, are the devices which are preferably deployed by story recipients as a minimal uptake to display affiliation, especially in the case of "scary" stories. During the course of conversational storytelling, it is structurally necessary that recipients warrant the tellers speakership. At the same time, a particular affective display-not only in response to what has been said but also to what comes at the climax-appears to become relevant. Immediately prior to the climax, when the teller employs elaborate multimodal cues, the recipient's display of an "anticipatory affect" is made relevant. A particular type of affect signals the anticipation of what kind of climax is approaching. The present paper explores how story recipients accomplish this two-fold task, namely to display alignment with the speaker's role allocation and listenership on the one hand and affiliation on the other. The study argues that a minimal uptake is called for, requiring only a minimal slot in the flow of storytelling and facial expressions that are most likely to fit this slot.}, language = {en} } @misc{LamprechtMargariaSteffen2009, author = {Lamprecht, Anna-Lena and Margaria, Tiziana and Steffen, Bernhard}, title = {Bio-jETI : a framework for semantics-based service composition}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-45066}, year = {2009}, abstract = {Background: The development of bioinformatics databases, algorithms, and tools throughout the last years has lead to a highly distributedworld of bioinformatics services. Without adequatemanagement and development support, in silico researchers are hardly able to exploit the potential of building complex, specialized analysis processes from these services. The Semantic Web aims at thoroughly equipping individual data and services with machine-processable meta-information, while workflow systems support the construction of service compositions. However, even in this combination, in silico researchers currently would have to deal manually with the service interfaces, the adequacy of the semantic annotations, type incompatibilities, and the consistency of service compositions. Results: In this paper, we demonstrate by means of two examples how Semantic Web technology together with an adequate domain modelling frees in silico researchers from dealing with interfaces, types, and inconsistencies. In Bio-jETI, bioinformatics services can be graphically combined to complex services without worrying about details of their interfaces or about type mismatches of the composition. These issues are taken care of at the semantic level by Bio-jETI's model checking and synthesis features. Whenever possible, they automatically resolve type mismatches in the considered service setting. Otherwise, they graphically indicate impossible/incorrect service combinations. In the latter case, the workflow developermay either modify his service composition using semantically similar services, or ask for help in developing the missing mediator that correctly bridges the detected type gap. Newly developed mediators should then be adequately annotated semantically, and added to the service library for later reuse in similar situations. Conclusion: We show the power of semantic annotations in an adequately modelled and semantically enabled domain setting. Using model checking and synthesis methods, users may orchestrate complex processes from a wealth of heterogeneous services without worrying about interfaces and (type) consistency. The success of this method strongly depends on a careful semantic annotation of the provided services and on its consequent exploitation for analysis, validation, and synthesis. We are convinced that these annotations will become standard, as they will become preconditions for the success and widespread use of (preferred) services in the Semantic Web}, language = {en} } @phdthesis{Zakrevskyy2006, author = {Zakrevskyy, Yuriy}, title = {Liquid crystallinity and alignment of ionic self-assembly complexes}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-10048}, school = {Universit{\"a}t Potsdam}, year = {2006}, abstract = {In this work the first observation of new type of liquid crystals is presented. This is ionic self-assembly (ISA) liquid crystals formed by introduction of oppositely charged ions between different low molecular tectonic units. As practically all conventional liquid crystals consist of rigid core and alkyl chains the attention is focused to the simplest case where oppositely charged ions are placed between a rigid core and alkyl tails. The aim of this work is to investigate and understand liquid crystalline and alignment properties of these materials. It was found that ionic interactions within complexes play the main role. Presence of these interactions restricts transition to isotropic phase. In addition, these interactions hold the system (like network) allowing crystallization into a single domain from aligned LC state. Alignment of these simple ISA complexes was spontaneous on a glass substrate. In order to show potentials for application perylenediimide and azobenzene containing ISA complexes have been investigated for correlations between phase behavior and their alignment properties. The best results of macroscopic alignment of perylenediimide-based ISA complexes have been obtained by zone-casting method. In the aligned films the columns of the complex align perpendicular to the phase-transition front. The obtained anisotropy (DR = 18) is thermally stable. The investigated photosensitive (azobenzene-based) ISA complexes show formation of columnar LC phases. It was demonstrated that photo alignment of such complexes was very effective (DR = 50 has been obtained). It was shown that photo-reorientation in the photosensitive ISA complexes is cooperative process. The size of domains has direct influence on efficiency of the photo-reorientation process. In the case of small domains the photo-alignment is the most effective. Under irradiation with linearly polarized light domains reorient in the plane of the film leading to macroscopic alignment of columns parallel to the light polarization and joining of small domains into big ones. Finally, the additional distinguishable properties of the ISA liquid crystalline complexes should be noted: (I) the complexes do not solve in water but readily solve in organic solvents; (II) the complexes have good film-forming properties when cast or spin-coated from organic solvent; (III) alignment of the complexes depends on their structure and secondary interactions between tectonic units.}, subject = {Fl{\"u}ssigkristall}, language = {en} }