@article{HildebrandtGeissler2012, author = {Hildebrandt, Niko and Geissler, Daniel}, title = {Semiconductor quantum dots as FRET acceptors for multiplexed diagnostics and molecular ruler application}, series = {Advances in Experimental Medicine and Biology}, volume = {733}, journal = {Advances in Experimental Medicine and Biology}, editor = {Zahavy, E and Ordentlich, A and Yitzhaki, S and Shafferman, A}, publisher = {Springer}, address = {Dordrecht}, isbn = {978-94-007-2554-6}, issn = {0065-2598}, doi = {10.1007/978-94-007-2555-3_8}, pages = {75 -- 86}, year = {2012}, abstract = {Applications based on Forster resonance energy transfer (FRET) play an important role for the determination of concentrations and distances within nanometer-scale systems in vitro and in vivo in many fields of biotechnology. Semiconductor nanocrystals (Quantum dots - QDs) possess ideal properties for their application as FRET acceptors when the donors have long excited state lifetimes and when direct excitation of QDs can be efficiently suppressed. Therefore, luminescent terbium complexes (LTCs) with excited state lifetimes of more than 2 ms are ideal FRET donor candidates for QD-acceptors. This chapter will give a short overview of theoretical and practical background of FRET, QDs and LTCs, and present some recent applications of LTC-QD FRET pairs for multiplexed ultra-sensitive in vitro diagnostics and nanometer-resolution molecular distance measurements.}, language = {en} } @article{VillatoroZuehlkeRiebeetal.2016, author = {Villatoro, Jos{\´e} Andr{\´e}s and Z{\"u}hlke, Martin and Riebe, Daniel and Beitz, Toralf and Weber, Marcus and Riedel, Jens and L{\"o}hmannsr{\"o}ben, Hans-Gerd}, title = {IR-MALDI ion mobility spectrometry: physical source characterization and application as HPLC detector}, series = {International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry}, volume = {19}, journal = {International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry}, publisher = {Springer}, address = {Heidelberg}, issn = {1435-6163}, doi = {10.1007/s12127-016-0208-1}, pages = {197 -- 207}, year = {2016}, abstract = {Infrared matrix-assisted laser dispersion and ionization (IR-MALDI) in combination with ion mobility (IM) spectrometry enables the direct analysis of biomolecules in aqueous solution. The release of ions directly from an aqueous solution is based on a phase explosion, induced by the absorption of an IR laser pulse, which disperses the liquid as vapor, nano-and micro-droplets. The ionization process is characterized initially by a broad spatial distribution of the ions, which is a result of complex fluid dynamics and desolvation kinetics. These processes have a profound effect on the shape and width of the peaks in the IM spectra. In this work, the transport of ions by the phase explosion-induced shockwave could be studied independently from the transport by the electric field. The shockwave-induced mean velocities of the ions at different time scales were determined through IM spectrometry and shadowgraphy. The results show a deceleration of the ions from 118 m.s(-1) at a distance of 400 mu m from the liquid surface to 7.1 m.s(-1) at a distance of 10 mm, which is caused by a pile-up effect. Furthermore, the desolvation kinetics were investigated and a first-order desolvation constant of 325 +/- 50 s(-1) was obtained. In the second part, the IR-MALDI-IM spectrometer is used as an HPLC detector for the two-dimensional separation of a pesticide mixture.}, language = {en} } @article{KellisEllinoudisIntziegianni2017, author = {Kellis, Eleftherios and Ellinoudis, Athanasios and Intziegianni, Konstantina}, title = {Reliability of sonographic assessment of biceps femoris distal tendon strain during passive stretching}, series = {Ultrasound in Medicine \& Biology}, volume = {43}, journal = {Ultrasound in Medicine \& Biology}, publisher = {Elsevier}, address = {New York}, issn = {0301-5629}, doi = {10.1016/j.ultrasmedbio.2017.04.018}, pages = {1769 -- 1779}, year = {2017}, abstract = {The purpose of this study was to determine the intra-rater, inter-examiner and inter-observer reliability of biceps femoris long head (BFlh) tendon strain using ultrasound imaging. Nineteen patients (age: 20.4 +/- 0.35 y) were tested twice with a 1-wk interval. Each session included passive stretching from three different hip positions. Tests were performed independently by two examiners while BFlh tendon displacement (mm) and strain (\%) were manually extracted from ultrasound video footages by two observers. Intra-rater comparisons revealed an intra-class correlation coefficient (ICC2,1) range of 0.87 to 0.98 and a variability less than 4.74\%. Interexaminer comparisons revealed an ICC2,1 range of 0.83 to 0.99 and less than 4.69\% variability. Inter-observer ICCs ranged from 0.93 to 0.97 with variability less than 4.89\%. Using a well-defined scanning protocol, two experienced examiners attained high levels of intra-rater agreement, with similarly excellent results for inter-rater and inter-observer reliability for BFlh tendon displacement and strain. (E-mail: ekellis@phed-sr.auth.gr) (C) 2017 World Federation for Ultrasound in Medicine \& Biology.}, language = {en} } @article{ZuehlkeZenichowskiRiebeetal.2017, author = {Z{\"u}hlke, Martin and Zenichowski, Karl and Riebe, Daniel and Beitz, Toralf and L{\"o}hmannsr{\"o}ben, Hans-Gerd}, title = {Subambient pressure electrospray ionization ion mobility spectrometry}, series = {International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry}, volume = {20}, journal = {International journal for ion mobility spectrometry : official publication of the International Society for Ion Mobility Spectrometry}, publisher = {Springer}, address = {Heidelberg}, issn = {1435-6163}, doi = {10.1007/s12127-017-0215-x}, pages = {47 -- 56}, year = {2017}, abstract = {The pressure dependence of sheath gas assisted electrospray ionization (ESI) was investigated based on two complementary experimental setups, namely an ESI-ion mobility (IM) spectrometer and an ESI capillary - Faraday plate setup housed in an optically accessible vacuum chamber. The ESI-IM spectrometer is capable of working in the pressure range between 300 and 1000 mbar. Another aim was the assessment of the analytical capabilities of a subambient pressure ESI-IM spectrometer. The pressure dependence of ESI was characterized by imaging the electrospray and recording current-voltage (I-U) curves. Qualitatively different behavior was observed in both setups. While the current rises continuously with the voltage in the capillary-plate setup, a sharp increase of the current was measured in the IM spectrometer above a pressure-dependent threshold voltage. The different character can be attributed to the detection of different species in both experiments. In the capillary-plate experiment, a multitude of charged species are detected while only desolvated ions attribute to the IM spectrometer signal. This finding demonstrates the utility of IM spectrometry for the characterization of ESI, since in contrast to the capillary-plate setup, the release of ions from the electrospray droplets can be observed. The I-U curves change significantly with pressure. An important result is the reduction of the maximum current with decreasing pressure. The connected loss of ionization efficiency can be compensated by a more efficient transfer of ions in the IM spectrometer at increased E/N. Thus, similar limits of detection could be obtained at 500 mbar and 1 bar.}, language = {en} } @article{KloseGuillemoteauSimonetal.2018, author = {Klose, Tim and Guillemoteau, Julien and Simon, Francois-Xavier and Tronicke, Jens}, title = {Toward subsurface magnetic permeability imaging with electromagnetic induction sensors}, series = {Geophysics}, volume = {83}, journal = {Geophysics}, number = {5}, publisher = {Society of Exploration Geophysicists}, address = {Tulsa}, issn = {0016-8033}, doi = {10.1190/GEO2017-0827.1}, pages = {E335 -- E345}, year = {2018}, abstract = {In near-surface geophysics, small portable loop-loop electro-magnetic induction (EMI) sensors using harmonic sources with a constant and rather small frequency are increasingly used to investigate the electrical properties of the subsurface. For such sensors, the influence of electrical conductivity and magnetic permeability on the EMI response is well-understood. Typically, data analysis focuses on reconstructing an electrical conductivity model by inverting the out-of-phase response. However, in a variety of near-surface applications, magnetic permeability (or susceptibility) models derived from the in-phase (IP) response may provide important additional information. In view of developing a fast 3D inversion procedure of the IP response for a dense grid of measurement points, we first analyze the 3D sensitivity functions associated with a homogeneous permeable half-space. Then, we compare synthetic data computed using a linear forward-modeling method based on these sensitivity functions with synthetic data computed using full nonlinear forward-modeling methods. The results indicate the correctness and applicability of our linear forward-modeling approach. Furthermore, we determine the advantages of converting IP data into apparent permeability, which, for example, allows us to extend the applicability of the linear forward-modeling method to high-magnetic environments. Finally, we compute synthetic data with the linear theory for a model consisting of a controlled magnetic target and compare the results with field data collected with a four-configuration loop-loop EMI sensor. With this field-scale experiment, we determine that our linear forward-modeling approach can reproduce measured data with sufficiently small error, and, thus, it represents the basis for developing efficient inversion approaches.}, language = {en} }