@article{SchenkFettkeLenzetal.2012,
  author    = {Schenk, J{\"o}rg A. and Fettke, J{\"o}rg and Lenz, Christine and Albers, Katharina and Mallwitz, Frank and Gajovic-Eichelmann, Nenad and Ehrentreich-F{\"o}rster, Eva and Kusch, Emely and Sellrie, Frank},
  title     = {Secretory leukocyte protease inhibitor (SLPI) might contaminate murine monoclonal antibodies after purification on protein G},
  series = {Journal of biotechnology},
  volume    = {158},
  journal   = {Journal of biotechnology},
  number    = {1-2},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0168-1656},
  doi       = {10.1016/j.jbiotec.2011.12.025},
  pages     = {34 -- 35},
  year      = {2012},
  abstract  = {The large scale production of a monoclonal anti-progesterone antibody in serum free medium followed by affinity chromatography on protein G lead to a contamination of the antibody sample with a protein of about 14 kDa. This protein was identified by mass spectrometry as secretory leukocyte protease inhibitor (SLPI). This SLPI contamination lead to a failure of the fiber-optic based competitive fluorescence assay to detect progesterone in milk. Purification of the monoclonal antibody using protein A columns circumvented this problem.},
  language  = {en}
}