@article{MesserschmidtHeilmann2013,
  author    = {Messerschmidt, Katrin and Heilmann, Katja},
  title     = {Toxin-antigen conjugates as selection tools for antibody producing cells},
  series = {Journal of immunological methods},
  volume    = {387},
  journal   = {Journal of immunological methods},
  number    = {1-2},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0022-1759},
  doi       = {10.1016/j.jim.2012.10.010},
  pages     = {167 -- 172},
  year      = {2013},
  abstract  = {The generation of antibodies with designated specificity requires cost-intensive and time-consuming screening procedures. Here we present a new method by which hybridoma cells can be selected based on the specificity of the produced antibody by the use of antigen-toxin-conjugates thus eliminating the need of a screening procedure. Initial experiments were done with methotrexate as low molecular weight toxin and fluorescein as model antigen. Methotrexate and a methotrexate-fluorescein conjugate were characterized regarding their toxicity. Afterwards the effect of the fluorescein-specific antibody B13-DE1 on the toxicity of the methotrexate-fluorescein conjugate was determined. Finally, first results showed that hybridoma cells that produce fluorescein specific antibodies are able to grow in the presence of fluorescein-toxin-conjugates.},
  language  = {en}
}
@article{MichelchenMicheelHanack2021,
  author    = {Michelchen, Sophia and Micheel, Burkhard and Hanack, Katja},
  title     = {In vitro immunization approach to generate specific murine monoclonal IgG antibodies},
  series = {Journal of immunological methods : JIM},
  volume    = {499},
  journal   = {Journal of immunological methods : JIM},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0022-1759},
  doi       = {10.1016/j.jim.2021.113149},
  pages     = {8},
  year      = {2021},
  abstract  = {Generating a monoclonal antibody to date is a time intense process that requires immunization of laboratory animals. The transfer of the humoral immune response into in vitro settings enables a shortening of this process and circumvents the necessity of in vivo immunization. However, to orchestrate the complex interplay of dendritic cells, T and B lymphocytes in vitro is very challenging. We therefore aimed for a simplified approach focusing on the protagonist of antibody production: the B lymphocyte. We activated purified murine B lymphocytes alone in vitro by using combinations of antigen and stimuli. We were able to induce a specific antibody response within ten days of culture against a viral coat protein as model antigen. Antibodies were of both IgM and IgG subclass. The stimulated B lymphocytes were transformed into permanently antibody-producing hybridomas by cell fusion technology. We furthermore used this method to induce a specific antibody response against L. pneumophila in vitro. We thus established a useful and effective in vitro protocol to generate monoclonal antibodies. By overcoming the necessity of in vivo immunization this protocol may be the first step towards a universal strategy to generate antibodies from various species.},
  language  = {en}
}