@article{LachmannMaberlySpijkerman2016,
  author    = {Lachmann, Sabrina C. and Maberly, Stephen C. and Spijkerman, Elly},
  title     = {ECOPHYSIOLOGY MATTERS: LINKING INORGANIC CARBON ACQUISITION TO ECOLOGICAL PREFERENCE IN FOUR SPECIES OF MICROALGAE (CHLOROPHYCEAE)},
  series = {Journal of phycology},
  volume    = {52},
  journal   = {Journal of phycology},
  publisher = {Wiley-Blackwell},
  address   = {Hoboken},
  issn      = {0022-3646},
  doi       = {10.1111/jpy.12462},
  pages     = {1051 -- 1063},
  year      = {2016},
  abstract  = {The effect of CO2 supply is likely to play an important role in algal ecology. Since inorganic carbon (C-i) acquisition strategies are very diverse among microalgae and C-i availability varies greatly within and among habitats, we hypothesized that C-i acquisition depends on the pH of their preferred natural environment (adaptation) and that the efficiency of C-i uptake is affected by CO2 availability (acclimation). To test this, four species of green algae originating from different habitats were studied. The pH-drift and C-i uptake kinetic experiments were used to characterize C-i acquisition strategies and their ability to acclimate to high and low CO2 conditions and high and low pH was evaluated. Results from pH drift experiments revealed that the acidophile and acidotolerant Chlamydomonas species were mainly restricted to CO2, whereas the two neutrophiles were efficient bicarbonate users. CO2 compensation points in low CO2-acclimated cultures ranged between 0.6 and 1.4 mu M CO2 and acclimation to different culture pH and CO2 conditions suggested that CO2 concentrating mechanisms were present in most species. High CO2 acclimated cultures adapted rapidly to low CO2 condition during pH-drifts. C-i uptake kinetics at different pH values showed that the affinity for C-i was largely influenced by external pH, being highest under conditions where CO2 dominated the C-i pool. In conclusion, C-i acquisition was highly variable among four species of green algae and linked to growth pH preference, suggesting that there is a connection between C-i acquisition and ecological distribution.},
  language  = {en}
}
@article{SpijkermanStojkovicHollandetal.2016,
  author    = {Spijkerman, Elly and Stojkovic, Slobodanka and Holland, Daryl and Lachmann, Sabrina C. and Beardall, John},
  title     = {Nutrient induced fluorescence transients (NIFTs) provide a rapid measure of P and C (co-)limitation in a green alga},
  series = {European journal of phycology},
  volume    = {51},
  journal   = {European journal of phycology},
  publisher = {Hindawi},
  address   = {Abingdon},
  issn      = {0967-0262},
  doi       = {10.1080/09670262.2015.1095355},
  pages     = {47 -- 58},
  year      = {2016},
  abstract  = {Nutrient Induced Fluorescence Transients (NIFTs) have been shown to be a possible way of testing for the limiting nutrient in algal populations. In this study we tested the hypothesis that NIFTs can be used to detect a (co-)limitation for inorganic phosphorus (Pi) and CO2 in the green alga Chlamydomonas acidophila and that the magnitude of the NIFTs can be related to cellular P:C ratios. We show a co-limitation response for Pi and CO2 via traditional nutrient enrichment experiments in natural phytoplankton populations dominated by C. acidophila. We measured NIFT responses after a Pi- or a CO2-spike in C. acidophila batch cultures at various stages of Pi and inorganic C limitation. Significant NIFTs were observed in response to spikes in both nutrients. The NIFT response to a Pi-spike showed a strong negative correlation with cellular P:C ratio that was pronounced below 3 mmol P: mol C (equivalent to 0.2 pg P cell(-1)). Both cellular P and C content influenced the extent of the Pi-NIFT response. The NIFT response to a CO2-spike correlated to low CO2 culturing conditions and also had a negative correlation with cellular P content. A secondary response within the Pi-NIFT response was related to the CO2 concentration and potentially reflected co-limitation. In conclusion, NIFTs provided a quick and reliable method to detect the growth-limiting nutrient in an extremophile green alga, under Pi-, CO2- and Pi/CO2 (co-)limited growth conditions.},
  language  = {en}
}