@article{TaubeGanzertGrossartetal.2017, author = {Taube, Robert and Ganzert, Lars and Grossart, Hans-Peter and Gleixner, Gerd and Premke, Katrin}, title = {Organic matter quality structures benthic fatty acid patterns and the abundance of fungi and bacteria in temperate lakes}, series = {The science of the total environment : an international journal for scientific research into the environment and its relationship with man}, volume = {610}, journal = {The science of the total environment : an international journal for scientific research into the environment and its relationship with man}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0048-9697}, doi = {10.1016/j.scitotenv.2017.07.256}, pages = {469 -- 481}, year = {2017}, abstract = {Benthic microbial communities (BMCs) play important roles in the carbon cycle of lakes, and benthic littoral zones in particular have been previously highlighted as biogeochemical hotspots. Dissolved organic matter (DOM) presents the major carbon pool in lakes, and although the effect of DOM composition on the pelagic microbial community composition is widely accepted, little is known about its effect on BMCs, particularly aquatic fungi. Therefore, we investigated the composition of benthic littoral microbial communities in twenty highly diverse lakes in northeast Germany. DOM quality was analyzed via size exclusion chromatography (SEC), fluorescence parallel factor analyses (PRAFACs) and UV-Vis spectroscopy. We determined the BMC composition and biomass using phospholipid-derived fatty acids (PLFA) and extended the interpretation to the analysis of fungi by applying a Bayesian mixed model. We present evidence that the quality of DOM structures the BMCs, which are dominated by heterotrophic bacteria and show low fungal biomass. The fungal biomass increases when the DOM pool is processed by microorganisms of allochthonous origin, whereas the opposite is true for bacteria.}, language = {en} } @article{VencesLyraKuenemanetal.2016, author = {Vences, Miguel and Lyra, Mariana L. and Kueneman, Jordan G. and Bletz, Molly C. and Archer, Holly M. and Canitz, Julia and Handreck, Svenja and Randrianiaina, Roger-Daniel and Struck, Ulrich and Bhuju, Sabin and Jarek, Michael and Geffers, Robert and McKenzie, Valerie J. and Tebbe, Christoph C. and Haddad, CLio F. B. and Glos, Julian}, title = {Gut bacterial communities across tadpole ecomorphs in two diverse tropical anuran faunas}, series = {The science of nature}, volume = {103}, journal = {The science of nature}, publisher = {Springer}, address = {Heidelberg}, issn = {0028-1042}, doi = {10.1007/s00114-016-1348-1}, pages = {68 -- 73}, year = {2016}, abstract = {Animal-associated microbial communities can play major roles in the physiology, development, ecology, and evolution of their hosts, but the study of their diversity has yet focused on a limited number of host species. In this study, we used high-throughput sequencing of partial sequences of the bacterial 16S rRNA gene to assess the diversity of the gut-inhabiting bacterial communities of 212 specimens of tropical anuran amphibians from Brazil and Madagascar. The core gut-associated bacterial communities among tadpoles from two different continents strongly overlapped, with eight highly represented operational taxonomic units (OTUs) in common. In contrast, the core communities of adults and tadpoles from Brazil were less similar with only one shared OTU. This suggests a community turnover at metamorphosis. Bacterial diversity was higher in tadpoles compared to adults. Distinct differences in composition and diversity occurred among gut bacterial communities of conspecific tadpoles from different water bodies and after experimental fasting for 8 days, demonstrating the influence of both environmental factors and food on the community structure. Communities from syntopic tadpoles clustered by host species both in Madagascar and Brazil, and the Malagasy tadpoles also had species-specific isotope signatures. We recommend future studies to analyze the turnover of anuran gut bacterial communities at metamorphosis, compare the tadpole core communities with those of other aquatic organisms, and assess the possible function of the gut microbiota as a reservoir for protective bacteria on the amphibian skin.}, language = {en} }