@article{ReinickeReesEspeeletal.2017,
  author    = {Reinicke, Stefan and Rees, Huw C. and Espeel, Pieter and Vanparijs, Nane and Bisterfeld, Carolin and Dick, Markus and Rosencrantz, Ruben R. and Brezesinski, Gerald and de Geest, Bruno G. and Du Prez, Filip E. and Pietruszka, J{\"o}rg and B{\"o}ker, Alexander},
  title     = {Immobilization of 2-Deoxy-D-ribose-5-phosphate Aldolase in Polymeric Thin Films via the Langmuir-Schaefer Technique},
  series = {ACS applied materials \& interfaces},
  volume    = {9},
  journal   = {ACS applied materials \& interfaces},
  publisher = {American Chemical Society},
  address   = {Washington},
  issn      = {1944-8244},
  doi       = {10.1021/acsami.6b13632},
  pages     = {8317 -- 8326},
  year      = {2017},
  abstract  = {A synthetic protocol for the fabrication of ultrathin polymeric films containing the enzyme 2-deoxy-D-ribose-5-phosphate aldolase from Escherichia coli (DERA(EC)) is presented. Ultrathin enzymatically active films are useful for applications in which only small quantities of active material are needed and at the same time quick response and contact times without diffusion limitation are wanted. We show how DERA as an exemplary enzyme can be immobilized in a thin polymer layer at the air-water interface and transferred to a suitable support by the Langmuir-Schaefer technique under full conservation of enzymatic activity. The polymer in use is a poly(N-isopropylacrylamide-co-N-2-thiolactone acrylamide) (P(NIPAAm-co-TlaAm)) statistical copolymer in which the thiolactone units serve a multitude of purposes including hydrophobization of the polymer, covalent binding of the enzyme and the support and finally cross-linking of the polymer matrix. The application of this type of polymer keeps the whole approach simple as additional cocomponents such as cross-linkers are avoided.},
  language  = {en}
}
@misc{WernerMuenzbergHassetal.2016,
  author    = {Werner, Peter and M{\"u}nzberg, Marvin and Hass, Roland and Reich, Oliver},
  title     = {Process analytical approaches for the coil-to-globule transition of poly(N-isopropylacrylamide) in a concentrated aqueous suspension},
  series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  journal   = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe},
  number    = {944},
  issn      = {1866-8372},
  doi       = {10.25932/publishup-43116},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-431162},
  pages     = {807 -- 819},
  year      = {2016},
  abstract  = {The coil-to-globule transition of poly(N-isopropylacrylamide) (PNIPAM) microgel particles suspended in water has been investigated in situ as a function of heating and cooling rate with four optical process analytical technologies (PAT), sensitive to structural changes of the polymer. Photon Density Wave (PDW) spectroscopy, Focused Beam Reflectance Measurements (FBRM), turbidity measurements, and Particle Vision Microscope (PVM) measurements are found to be powerful tools for the monitoring of the temperature-dependent transition of such thermo-responsive polymers. These in-line technologies allow for monitoring of either the reduced scattering coefficient and the absorption coefficient, the chord length distribution, the reflected intensities, or the relative backscatter index via in-process imaging, respectively. Varying heating and cooling rates result in rate-dependent lower critical solution temperatures (LCST), with different impact of cooling and heating. Particularly, the data obtained by PDW spectroscopy can be used to estimate the thermodynamic transition temperature of PNIPAM for infinitesimal heating or cooling rates. In addition, an inverse hysteresis and a reversible building of micrometer-sized agglomerates are observed for the PNIPAM transition process.},
  language  = {en}
}
@phdthesis{Tan2011,
  author    = {Tan, Irene},
  title     = {Towards greener stationary phases : thermoresponsive and carbonaceous chromatographic supports},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-53130},
  school      = {Universit{\"a}t Potsdam},
  year      = {2011},
  abstract  = {Polymers which are sensitive towards external physical, chemical and electrical stimuli are termed as 'intelligent materials' and are widely used in medical and engineering applications. Presently, polymers which can undergo a physical change when heat is applied at a certain temperature (cloud point) in water are well-studied for this property in areas of separation chemistry, gene and drug delivery and as surface modifiers. One example of such a polymer is the poly (N-isopropylacrylamide) PNIPAAM, where it is dissolved well in water below 32 oC, while by increasing the temperature further leads to its precipitation. In this work, an alternative polymer poly (2-(2-methoxy ethoxy)ethyl methacrylate-co- oligo(ethylene glycol) methacrylate) (P(MEO2MA-co-OEGMA)) is studied due to its biocompatibility and the ability to vary its cloud points in water. When a layer of temperature responsive polymer was attached to a single continuous porous piece of silica-based material known as a monolith, the thermoresponsive characteristic was transferred to the column surfaces. The hybrid material was demonstrated to act as a simple temperature 'switch' in the separation of a mixture of five steroids under water. Different analytes were observed to be separated under varying column temperatures. Furthermore, more complex biochemical compounds such as proteins were also tested for separation. The importance of this work is attributed to separation processes utilizing environmentally friendly conditions, since harsh chemical environments conventionally used to resolve biocompounds could cause their biological activities to be rendered inactive.},
  language  = {en}
}