@misc{WestendorfBaeErlenkamperetal.2010, author = {Westendorf, Christian and Bae, Albert J. and Erlenkamper, Christoph and Galland, Edouard and Franck, Carl and Bodenschatz, Eberhard and Beta, Carsten}, title = {Live cell flattening}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, number = {835}, issn = {1866-8372}, doi = {10.25932/publishup-42831}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-428311}, pages = {17}, year = {2010}, abstract = {Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis and in vivo actin polymerization, have been investigated using these techniques. Here, we review the well known agar overlayer protocol and the oil overlay method. In addition, we present more elaborate microfluidics-based techniques that provide us with a greater level of control. We demonstrate these techniques on the social amoebae Dictyostelium discoideum, comparing the advantages and disadvantages of each method.}, language = {en} } @misc{Beta2010, author = {Beta, Carsten}, title = {Bistability in the actin cortex}, series = {Postprints der Universit{\"a}t Potsdam Mathematisch-Naturwissenschaftliche Reihe}, volume = {3}, journal = {Postprints der Universit{\"a}t Potsdam Mathematisch-Naturwissenschaftliche Reihe}, number = {12}, doi = {10.25932/publishup-42938}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-429385}, pages = {9}, year = {2010}, abstract = {Multi-color fluorescence imaging experiments of wave forming Dictyostelium cells have revealed that actin waves separate two domains of the cell cortex that differ in their actin structure and phosphoinositide composition. We propose a bistable model of actin dynamics to account for these experimental observation. The model is based on the simplifying assumption that the actin cytoskeleton is composed of two distinct network types, a dendritic and a bundled network. The two structurally different states that were observed in experiments correspond to the stable fixed points in the bistable regime of this model. Each fixed point is dominated by one of the two network types. The experimentally observed actin waves can be considered as trigger waves that propagate transitions between the two stable fixed points.}, language = {en} }