@article{GrauHacklKoepflietal.2018, author = {Grau, Jos{\´e} Horacio and Hackl, Thomas and Koepfli, Klaus-Peter and Hofreiter, Michael}, title = {Improving draft genome contiguity with reference-derived in silico mate-pair libraries}, series = {GigaScience}, volume = {7}, journal = {GigaScience}, number = {5}, publisher = {Oxford University Press}, address = {Oxford}, issn = {2047-217X}, doi = {10.1093/gigascience/giy029}, pages = {1 -- 6}, year = {2018}, abstract = {Background Contiguous genome assemblies are a highly valued biological resource because of the higher number of completely annotated genes and genomic elements that are usable compared to fragmented draft genomes. Nonetheless, contiguity is difficult to obtain if only low coverage data and/or only distantly related reference genome assemblies are available. Findings In order to improve genome contiguity, we have developed Cross-Species Scaffolding—a new pipeline that imports long-range distance information directly into the de novo assembly process by constructing mate-pair libraries in silico. Conclusions We show how genome assembly metrics and gene prediction dramatically improve with our pipeline by assembling two primate genomes solely based on ∼30x coverage of shotgun sequencing data.}, language = {en} } @misc{GrauHacklKoepflietal.2018, author = {Grau, Jos{\´e} Horacio and Hackl, Thomas and Koepfli, Klaus-Peter and Hofreiter, Michael}, title = {Improving draft genome contiguity with reference-derived in silico mate-pair libraries}, series = {GigaScience}, journal = {GigaScience}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-419225}, pages = {6}, year = {2018}, abstract = {Background Contiguous genome assemblies are a highly valued biological resource because of the higher number of completely annotated genes and genomic elements that are usable compared to fragmented draft genomes. Nonetheless, contiguity is difficult to obtain if only low coverage data and/or only distantly related reference genome assemblies are available. Findings In order to improve genome contiguity, we have developed Cross-Species Scaffolding—a new pipeline that imports long-range distance information directly into the de novo assembly process by constructing mate-pair libraries in silico. Conclusions We show how genome assembly metrics and gene prediction dramatically improve with our pipeline by assembling two primate genomes solely based on ∼30x coverage of shotgun sequencing data.}, language = {en} } @article{Hermanns2020, author = {Hermanns, Jolanda}, title = {Scaffolding for chemistry students - which tools are assessed as being more helpful}, series = {Chemistry Teacher International}, journal = {Chemistry Teacher International}, publisher = {De Gruyter}, address = {Berlin}, issn = {2569-3263}, doi = {10.1515/cti-2020-0019}, pages = {10}, year = {2020}, abstract = {In this paper the use of two different scaffolds in a seminar on the topic of heterocycles is discussed. The students first used both scaffolds (stepped supporting tools and a task navigator) on two tasks and could then choose for one other task the scaffold that suited them more. The scaffolds were evaluated in a mixedmethods study by the use of questionnaires and the conducting of a focus group interview. Both scaffolds were assessed as being helpful. However, students who thought they didn't need different sorts of tips, as provided by the task navigator, chose the stepped supporting tools. All students reflected on their use of the scaffolds; their choices for one of both are therefore well-founded. As the reasons for choosing the scaffold are very individual, in future seminars both types of scaffolds will be provided.}, language = {en} } @misc{Hermanns2020, author = {Hermanns, Jolanda}, title = {Scaffolding for chemistry students - which tools are assessed as being more helpful}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch-Naturwissenschaftliche Reihe}, number = {1108}, issn = {1866-8372}, doi = {10.25932/publishup-49100}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-491001}, pages = {12}, year = {2020}, abstract = {In this paper the use of two different scaffolds in a seminar on the topic of heterocycles is discussed. The students first used both scaffolds (stepped supporting tools and a task navigator) on two tasks and could then choose for one other task the scaffold that suited them more. The scaffolds were evaluated in a mixedmethods study by the use of questionnaires and the conducting of a focus group interview. Both scaffolds were assessed as being helpful. However, students who thought they didn't need different sorts of tips, as provided by the task navigator, chose the stepped supporting tools. All students reflected on their use of the scaffolds; their choices for one of both are therefore well-founded. As the reasons for choosing the scaffold are very individual, in future seminars both types of scaffolds will be provided.}, language = {en} }