@misc{ZemellaThoringHoffmeisteretal.2018, author = {Zemella, Anne and Thoring, Lena and Hoffmeister, Christian and Šamal{\´i}kov{\´a}, M{\´a}ria and Ehren, Patricia and W{\"u}stenhagen, Doreen Anja and Kubick, Stefan}, title = {Cell-free protein synthesis as a novel tool for directed glycoengineering of active erythropoietin}, series = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, journal = {Postprints der Universit{\"a}t Potsdam : Mathematisch Naturwissenschaftliche Reihe}, number = {824}, doi = {10.25932/publishup-42701}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-427017}, pages = {14}, year = {2018}, abstract = {As one of the most complex post-translational modification, glycosylation is widely involved in cell adhesion, cell proliferation and immune response. Nevertheless glycoproteins with an identical polypeptide backbone mostly differ in their glycosylation patterns. Due to this heterogeneity, the mapping of different glycosylation patterns to their associated function is nearly impossible. In the last years, glycoengineering tools including cell line engineering, chemoenzymatic remodeling and site-specific glycosylation have attracted increasing interest. The therapeutic hormone erythropoietin (EPO) has been investigated in particular by various groups to establish a production process resulting in a defined glycosylation pattern. However commercially available recombinant human EPO shows batch-to-batch variations in its glycoforms. Therefore we present an alternative method for the synthesis of active glycosylated EPO with an engineered O-glycosylation site by combining eukaryotic cell-free protein synthesis and site-directed incorporation of non-canonical amino acids with subsequent chemoselective modifications.}, language = {en} } @misc{SanderEccardHeim2017, author = {Sander, Martha Maria and Eccard, Jana and Heim, Wieland}, title = {Flight range estimation of migrant Yellow-browed Warblers Phylloscopus inornatus on the East Asian flyway}, series = {Bird Study}, journal = {Bird Study}, url = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus4-412823}, pages = {5}, year = {2017}, abstract = {Fat loads were quantified for 2125 Yellow-browed Warblers Phylloscopus inornatus trapped at a stop-over site in Far East Russia during autumn migration. Flight ranges of 660-820 km were estimated for the fattest individuals, suggesting that they would need to stop for refuelling at least six times to reach their wintering areas in South East Asia.}, language = {en} }