@misc{KortPeterKoopmanschap1983,
  author    = {Kort, C. A. D. de and Peter, Martin G. and Koopmanschap, A. B.},
  title     = {Binding and degradation of juvenile hormone III by haemolymph proteins of the Colorado potato beetle: a re-examination},
  url       = {http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-16777},
  year      = {1983},
  abstract  = {The haemolymph of the adult Colorado potato beetle, Lepinotarsa decemlineata Say, contains a high molecular weight (MW > 200,000) JH-III specific binding protein. The Kd value of the protein for racemic JH-III is 1.3 ± 0.2 × 10-7 M. It has a lower affinity for racemic JH-I and it does not bind JH-III-diol or JH-III-acid. The binding protein does discriminate between the enantiomers of synthetic, racemic JH-III as was determined by stereochemical anaysis of the bound and the free JH-III. Incubation of racemic JH-III with crude haemolymph results in preferential formation of (10S)-JH-III-acid, the unnatural configuration. The JH-esterase present in L. decemlineata haemolymph is not enantioselective. It is concluded that the most important function of the binding protein is that of a specific carrier, protecting the natural hormone against degradation by esterases. The carrier does not protect JH-I as efficiently as the lower homologue.},
  language  = {en}
}