@article{RosencrantzVuHoaNguyenParketal.2016, author = {Rosencrantz, Ruben R. and Vu Hoa Nguyen, and Park, Hyunji and Schulte, Christine and B{\"o}ker, Alexander and Schnakenberg, Uwe and Elling, Lothar}, title = {Lectin binding studies on a glycopolymer brush flow-through biosensor by localized surface plasmon resonance}, series = {Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry and Analusis}, volume = {408}, journal = {Analytical and bioanalytical chemistry : a merger of Fresenius' journal of analytical chemistry and Analusis}, publisher = {Springer}, address = {Heidelberg}, issn = {1618-2642}, doi = {10.1007/s00216-016-9667-9}, pages = {5633 -- 5640}, year = {2016}, abstract = {A localized surface plasmon resonance biosensor in a flow-through configuration was applied for investigating kinetics of lectin binding to surface-grafted glycopolymer brushes. Polycarbonate filter membranes with pore sizes of 400 nm were coated with a 114-nm thick gold layer and used as substrate for surface-initiated atom-transfer radical polymerization of a glycomonomer. These grafted from glycopolymer brushes were further modified with two subsequent enzymatic reactions on the surface to yield an immobilized trisaccharide presenting brush. Specific binding of lectins including Clostridium difficile toxin A receptor domain to the glycopolymer brush surface could be investigated in a microfluidic setup with flow-through of the analytes and transmission surface plasmon resonance spectroscopy.}, language = {en} } @article{HasinovicFribergKovachetal.2014, author = {Hasinovic, H. and Friberg, S. E. and Kovach, Ildyko and Koetz, Joachim}, title = {Destabilization of a dual emulsion to form a Janus emulsion}, series = {Colloid and polymer science : official journal of the Kolloid-Gesellschaft}, volume = {292}, journal = {Colloid and polymer science : official journal of the Kolloid-Gesellschaft}, number = {9}, publisher = {Springer}, address = {New York}, issn = {0303-402X}, doi = {10.1007/s00396-014-3263-3}, pages = {2319 -- 2324}, year = {2014}, abstract = {A vegetable oil (VO) was added to an emulsion of silicone oil in water (SO/W) with mixing limited to once turning the test tube upside down. Initially, the VO was dispersed into virtually centimeter-sized drops and the emulsion contained effectively no Janus drops, while after 1 h of agitation at a low level to prevent creaming, drops of 50-100-mu m size of the two oils were observed: in addition to an insignificant number of Janus drops. The topology of the latter showed them to emanate from flocculated individual drops of the two oils, but with no discernible effect by the interfacial tension equilibrium on the drop topology. Continued gentle mixing gave increasing fraction of Janus drops of increased size with a topology gradually approaching the one expected from the interfacial equilibrium at the contact line. The spontaneous formation of Janus drops indicated a reduction of the interfacial free energy in the process and the interfacial energy difference between separate and Janus drops was calculated for an appropriate range of interfacial tensions and for all oil fractions. The calculations enabled a distinction of the decrease due to interfacial area changes from the reduction of interfacial tensions per se, with the latter only a minor fraction.}, language = {en} } @misc{BetaBodenschatz2011, author = {Beta, Carsten and Bodenschatz, Eberhard}, title = {Microfluidic tools for quantitative studies of eukaryotic chemotaxis}, series = {European journal of cell biology}, volume = {90}, journal = {European journal of cell biology}, number = {10}, publisher = {Elsevier}, address = {Jena}, issn = {0171-9335}, doi = {10.1016/j.ejcb.2011.05.006}, pages = {811 -- 816}, year = {2011}, abstract = {Over the past decade, microfluidic techniques have been established as a versatile platform to perform live cell experiments under well-controlled conditions. To investigate the directional responses of cells, stable concentration profiles of chemotactic factors can be generated in microfluidic gradient mixers that provide a high degree of spatial control. However, the times for built-up and switching of gradient profiles are in general too slow to resolve the intracellular protein translocation events of directional sensing of eukaryotes. Here, we review an example of a conventional microfluidic gradient mixer as well as the novel flow photolysis technique that achieves an increased temporal resolution by combining the photo-activation of caged compounds with the advantages of microfluidic chambers.}, language = {en} }