@article{GerhardtEckeWalzetal.2014,
  author    = {Gerhardt, Matthias and Ecke, Mary and Walz, Michael and Stengl, Andreas and Beta, Carsten and Gerisch, G{\"u}nther},
  title     = {Actin and PIP3 waves in giant cells reveal the inherent length scale of an excited state},
  series = {Journal of cell science},
  volume    = {127},
  journal   = {Journal of cell science},
  number    = {20},
  publisher = {Company of Biologists Limited},
  address   = {Cambridge},
  issn      = {0021-9533},
  doi       = {10.1242/jcs.156000},
  pages     = {4507 -- 4517},
  year      = {2014},
  abstract  = {The membrane and actin cortex of a motile cell can autonomously differentiate into two states, one typical of the front, the other of the tail. On the substrate-attached surface of Dictyostelium discoideum cells, dynamic patterns of front-like and tail-like states are generated that are well suited to monitor transitions between these states. To image large-scale pattern dynamics independently of boundary effects, we produced giant cells by electric-pulse-induced cell fusion. In these cells, actin waves are coupled to the front and back of phosphatidylinositol (3,4,5)-trisphosphate (PIP3)-rich bands that have a finite width. These composite waves propagate across the plasma membrane of the giant cells with undiminished velocity. After any disturbance, the bands of PIP3 return to their intrinsic width. Upon collision, the waves locally annihilate each other and change direction; at the cell border they are either extinguished or reflected. Accordingly, expanding areas of progressing PIP3 synthesis become unstable beyond a critical radius, their center switching from a front-like to a tail-like state. Our data suggest that PIP3 patterns in normal-sized cells are segments of the self-organizing patterns that evolve in giant cells.},
  language  = {en}
}