TY  - JOUR
A1  - Welke, Robert-William
A1  - Sperber, Hannah Sabeth
A1  - Bergmann, Ronny
A1  - Koikkarah, Amit
A1  - Menke, Laura
A1  - Sieben, Christian
A1  - Krüger, Detlev H.
A1  - Chiantia, Salvatore
A1  - Herrmann, Andreas
A1  - Schwarzer, Roland
T1  - Characterization of hantavirus N protein intracellular dynamics and localization
T2  - Viruses
N2  - Hantaviruses are enveloped viruses that possess a tri-segmented, negative-sense RNA genome. 
The viral S-segment encodes the multifunctional nucleocapsid protein (N), which is involved in genome packaging, intracellular protein transport, immunoregulation, and several other crucial processes during hantavirus infection. 

In this study, we generated fluorescently tagged N protein constructs derived from Puumalavirus (PUUV), the dominant hantavirus species in Central, Northern, and Eastern Europe. 
We comprehensively characterized this protein in the rodent cell line CHO-K1, monitoring the dynamics of N protein complex formation and investigating co-localization with host proteins as well as the viral glycoproteins Gc and Gn. 
We observed formation of large, fibrillar PUUV N protein aggregates, rapidly coalescing from early punctate and spike-like assemblies. 
Moreover, we found significant spatial correlation of N with vimentin, actin, and P-bodies but not with microtubules. N constructs also co-localized with Gn and Gc albeit not as strongly as the glycoproteins associated with each other. 

Finally, we assessed oligomerization of N constructs, observing efficient and concentration-dependent multimerization, with complexes comprising more than 10 individual proteins.
KW  - hantavirus
KW  - N protein
KW  - oligomerization
KW  - actin
KW  - P-bodies
KW  - vimentin
KW  - Number and Brightness
KW  - Puumalavirus
KW  - macromolecular assemblies
Y1  - 2022
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/64191
SN  - 1999-4915
VL  - 14
IS  - 3
PB  - MDPI
CY  - Basel
ER  -