TY  - JOUR
A1  - Vogt, Birgit
A1  - Warncke, Marit
A1  - Micheel, Burkhard
A1  - Sheriff, Ahmed
T1  - Lentiviral gene transfer of CTLA4 generates B cells with reduced costimulatory properties : brief definite report
N2  - Peripheral T-cell (TC) tolerance can be induced by tolerogenic antigen-presenting cell (APC). A prerequisite is the reduction or blockade of B7 of APC. Besides dendritic cell, B cells can be used as APC. Here, we show the generation B cells with reduced B7 expression by lentiviral transduction of endoplasmic reticulum (ER)-directed CTLA4. Vectors coding for the human CTL4-Ig were used for the human B-cell line Raji. Transduction efficiency was over 90% (MOI = 3). For the murine B-cell line A20 and for primary mouse B cells, murine CTLA4 was used. We show that B cells with reduced B7 expression reduce the antigen (Ag) specific TC proliferation in vitro. B cells expressing an ER-directed CTLA4 may reduce Ag-specific immune responses.
Y1  - 2009
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/31981
UR  - http://informahealthcare.com/loi/aut
SN  - 0891-6934
ER  -