TY  - JOUR
A1  - Delfan, Maryam
A1  - Juybari, Raheleh Amadeh
A1  - Gorgani-Firuzjaee, Sattar
A1  - Nielsen, Jens Høiriis
A1  - Delfan, Neda
A1  - Laher, Ismail
A1  - Saeidi, Ayoub
A1  - Granacher, Urs
A1  - Zouhal, Hassane
T1  - High-Intensity Interval Training Improves Cardiac Function by miR-206 Dependent HSP60 Induction in Diabetic Rats
T2  - Frontiers in Cardiovascular Medicine
N2  - Objective: A role for microRNAs is implicated in several biological and pathological processes. We investigated the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on molecular markers of diabetic cardiomyopathy in rats.

Methods: Eighteen male Wistar rats (260 ± 10 g; aged 8 weeks) with streptozotocin (STZ)-induced type 1 diabetes mellitus (55 mg/kg, IP) were randomly allocated to three groups: control, MICT, and HIIT. The two different training protocols were performed 5 days each week for 5 weeks. Cardiac performance (end-systolic and end-diastolic dimensions, ejection fraction), the expression of miR-206, HSP60, and markers of apoptosis (cleaved PARP and cytochrome C) were determined at the end of the exercise interventions.

Results: Both exercise interventions (HIIT and MICT) decreased blood glucose levels and improved cardiac performance, with greater changes in the HIIT group (p < 0.001, η2: 0.909). While the expressions of miR-206 and apoptotic markers decreased in both training protocols (p < 0.001, η2: 0.967), HIIT caused greater reductions in apoptotic markers and produced a 20% greater reduction in miR-206 compared with the MICT protocol (p < 0.001). Furthermore, both training protocols enhanced the expression of HSP60 (p < 0.001, η2: 0.976), with a nearly 50% greater increase in the HIIT group compared with MICT.

Conclusions: Our results indicate that both exercise protocols, HIIT and MICT, have the potential to reduce diabetic cardiomyopathy by modifying the expression of miR-206 and its downstream targets of apoptosis. It seems however that HIIT is even more effective than MICT to modulate these molecular markers.
KW  - diabetes
KW  - apoptosis
KW  - miRNAs
KW  - exercise
KW  - cardiomyopathy
Y1  - 2022
UR  - https://publishup.uni-potsdam.de/frontdoor/index/index/docId/56722
SN  - 2297-055X
VL  - 9
SP  - 1
EP  - 11
PB  - Frontiers
CY  - Lausanne, Schweiz
ER  -